Cerium Oxide Nanoparticles are Nontoxic for Mouse Embryogenesis In Vitro and In Vivo

Cerium oxide nanoparticles (nanoceria) are considered as one of the most promising nanomaterials for biomedical applications. Complex analysis of cytotoxicity, including the assessment of effects on the reproductive system, is required for development of new biomedical materials. In this study, we investigated the effects of ultra-small citrate-stabilized cerium oxide nanoparticles on the development of mouse embryos in vitro and embryogenesis process in vivo. We have shown that nanoceria in a wide range of concentrations do not exert a toxic effect on the development of 2-cell embryos and embryogenesis.

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Cerium Oxide Nanoparticles: A Brief Review of Their Synthesis Methods and Biomedical Applications

Antioxidants ◽

10.3390/antiox7080097 ◽

2018 ◽

Vol 7 (8) ◽

pp. 97 ◽

Cited By ~ 94

Author(s):

Atul Dhall ◽

William Self

Keyword(s):

Cerium Oxide ◽

Biomedical Applications ◽

Antioxidant Properties ◽

Cerium Oxide Nanoparticles ◽

Oxide Nanoparticles ◽

Synthesis Methods ◽

Ros Scavenging ◽

Corona Formation

Cerium oxide nanoparticles (CeNPs) exhibit antioxidant properties both in vitro and in vivo. This is due to the self-regeneration of their surface, which is based on redox-cycling between 3+ and 4+ states for cerium, in response to their immediate environment. Additionally, oxygen vacancies in the lattice structure allow for alternating between CeO2 and CeO2−x during redox reactions. Research to identify and characterize the biomedical applications of CeNPs has been heavily focused on investigating their use in treating diseases that are characterized by higher levels of reactive oxygen species (ROS). Although the bio-mimetic activities of CeNPs have been extensively studied in vitro, in vivo interactions and associated protein corona formation are not well understood. This review describes: (1) the methods of synthesis for CeNPs, including the recent green synthesis methods that offer enhanced biocompatibility and a need for establishing a reference CeNP material for consistency across studies; (2) their enzyme-mimetic activities, with a focus on their antioxidant activities; and, (3) recent experimental evidence that demonstrates their ROS scavenging abilities and their potential use in personalized medicine.

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In Vitro and In Vivo Antioxidant Activity of the New Magnetic-Cerium Oxide Nanoconjugates

Nanomaterials ◽

10.3390/nano9111565 ◽

2019 ◽

Vol 9 (11) ◽

pp. 1565 ◽

Cited By ~ 7

Author(s):

Turin-Moleavin ◽

Fifere ◽

Lungoci ◽

Rosca ◽

Coroaba ◽

...

Keyword(s):

Oxidative Stress ◽

Antioxidant Activity ◽

Iron Oxide ◽

Cerium Oxide ◽

Chemical Activation ◽

Radical Scavenging ◽

Cerium Oxide Nanoparticles ◽

Oxide Nanoparticles

Background. Cerium oxide nanoparticles present the mimetic activity of superoxide dismutase, being able to inactivate the excess of reactive oxygen species (ROS) correlated with a large number of pathologies, such as stents restenosis and the occurrence of genetic mutations that can cause cancer. This study presents the synthesis and biological characterisation of nanoconjugates based on nanoparticles of iron oxide interconnected with cerium oxide conjugates. Methods. The synthesis of magnetite-nanoceria nanoconjugates has been done in several stages, where the key to the process is the coating of nanoparticles with polyethyleneimine and its chemical activation-reticulation with glutaraldehyde. The nanoconjugates are characterised by several techniques, and the antioxidant activity was evaluated in vitro and in vivo. Results. Iron oxide nanoparticles interconnected with cerium oxide nanoparticles were obtained, having an average diameter of 8 nm. Nanoconjugates prove to possess superparamagnetic properties and the saturation magnetisation varies with the addition of diamagnetic components in the system, remaining within the limits of biomedical applications. In vitro free-radical scavenging properties of nanoceria are improved after the coating of nanoparticles with polyethylenimine and conjugation with magnetite nanoparticles. In vivo studies reveal increased antioxidant activity in all organs and fluids collected from mice, which demonstrates the ability of the nanoconjugates to reduce oxidative stress. Conclusion. Nanoconjugates possess magnetic properties, being able to scavenge free radicals, reducing the oxidative stress. The combination of the two properties mentioned above makes them excellent candidates for theranostic applications.

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Detrimental effects of cerium oxide nanoparticles on testis, sperm parameters quality, and in vitro fertilization in mice: An experimental study

International Journal of Reproductive BioMedicine ◽

10.18502/ijrm.v19i9.9712 ◽

2021 ◽

pp. 801-810

Author(s):

Elnaz Hosseinalipour ◽

Mojtaba Karimipour ◽

Abbas Ahmadi

Keyword(s):

In Vitro Fertilization ◽

Cerium Oxide ◽

Reproductive System ◽

Male Reproductive System ◽

Sperm Parameters ◽

Sperm Chromatin ◽

Cerium Oxide Nanoparticles ◽

Oxide Nanoparticles ◽

Vitro Fertilization

Background: Cerium oxide nanoparticles (CeO2 NPs) as an important nanomaterial have a wide range of applications in many fields and human beings’ exposure to this nanomaterial is unavoidable. The effects of CeO2 NPs on the male reproductive system are controversial. Objective: To determine the effects of the administration of CeO2 NPs on the testis tissue, sperm parameters, and in vitro fertilization (IVF) in mice. Materials and Methods: Twenty-four male mice were divided into three groups (n = 8/each): one control and two experimental groups receiving CeO2 NPs at doses of 50 and 100 mg/kg body weight, respectively, for 35 days. At the end of the experiment, the diameter of seminiferous tubules (SNTs), epithelial height of SNTs, spermiogenesis index in testes, sperm parameters (count, motility, viability, and morphology), sperm chromatin condensation, DNA integrity, and IVF assays were analyzed. Results: Histological results showed that the tubular diameter, the epithelial height of the SNTs, and the spermiogenesis index were significantly decreased in the experimental groups receiving CeO2 NPs. All sperm parameters in the experimental groups were significantly reduced and, additionally, the percentages of immature sperms and sperms with DNA damage were significantly increased in groups treated with CeO2 NPs compared to the control. Furthermore, the rates of IVF and in vitro embryo development were decreased. Conclusion: Collectively, the current study showed that oral administration of CeO2 NPs in mice had detrimental effects on the male reproductive system through inducing testicular tissue alterations, decreasing sperm parameters quality, and also diminishing the IVF rate and in vitro embryonic development. Key words: Cerium oxide, Testis, Sperm, Fertilization, Mice.

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Cerium Oxide Nanoparticles Improve Outcome after In Vitro and In Vivo Mild Traumatic Brain Injury

Journal of Neurotrauma ◽

10.1089/neu.2016.4644 ◽

2020 ◽

Vol 37 (12) ◽

pp. 1452-1462 ◽

Cited By ~ 14

Author(s):

Zachary S. Bailey ◽

Eric Nilson ◽

John A. Bates ◽

Adewole Oyalowo ◽

Kevin S. Hockey ◽

...

Keyword(s):

Traumatic Brain Injury ◽

Brain Injury ◽

Cerium Oxide ◽

Mild Traumatic Brain Injury ◽

Cerium Oxide Nanoparticles ◽

Oxide Nanoparticles ◽

Improve Outcome

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Radioprotective effects of ultra-small citrate-stabilized cerium oxide nanoparticles in vitro and in vivo

RSC Advances ◽

10.1039/c6ra18566e ◽

2016 ◽

Vol 6 (108) ◽

pp. 106141-106149 ◽

Cited By ~ 22

Author(s):

A. L. Popov ◽

S. I. Zaichkina ◽

N. R. Popova ◽

O. M. Rozanova ◽

S. P. Romanchenko ◽

...

Keyword(s):

Cerium Oxide ◽

Cerium Oxide Nanoparticles ◽

Oxide Nanoparticles ◽

Action Mechanisms ◽

Radioprotective Action

Different radioprotective action mechanisms of CeO2 nanoparticles in vitro and in vivo are demonstrated and discussed.

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Cerium oxide nanoparticles improve liver regeneration after acetaminophen-induced liver injury and partial hepatectomy in rats

Journal of Nanobiotechnology ◽

10.1186/s12951-019-0544-5 ◽

2019 ◽

Vol 17 (1) ◽

Cited By ~ 7

Author(s):

Bernat Córdoba-Jover ◽

Altamira Arce-Cerezo ◽

Jordi Ribera ◽

Montse Pauta ◽

Denise Oró ◽

...

Keyword(s):

Liver Injury ◽

Liver Regeneration ◽

Cerium Oxide ◽

Partial Hepatectomy ◽

Experimental Models ◽

Cerium Oxide Nanoparticles ◽

Hepatic Regeneration ◽

Oxide Nanoparticles ◽

Acetyl Cysteine

Abstract Background and aims Cerium oxide nanoparticles are effective scavengers of reactive oxygen species and have been proposed as a treatment for oxidative stress-related diseases. Consequently, we aimed to investigate the effect of these nanoparticles on hepatic regeneration after liver injury by partial hepatectomy and acetaminophen overdose. Methods All the in vitro experiments were performed in HepG2 cells. For the acetaminophen and partial hepatectomy experimental models, male Wistar rats were divided into three groups: (1) nanoparticles group, which received 0.1 mg/kg cerium nanoparticles i.v. twice a week for 2 weeks before 1 g/kg acetaminophen treatment, (2) N-acetyl-cysteine group, which received 300 mg/kg of N-acetyl-cysteine i.p. 1 h after APAP treatment and (3) partial hepatectomy group, which received the same nanoparticles treatment before partial hepatectomy. Each group was matched with vehicle-controlled rats. Results In the partial hepatectomy model, rats treated with cerium oxide nanoparticles showed a significant increase in liver regeneration, compared with control rats. In the acetaminophen experimental model, nanoparticles and N-acetyl-cysteine treatments decreased early liver damage in hepatic tissue. However, only the effect of cerium oxide nanoparticles was associated with a significant increment in hepatocellular proliferation. This treatment also reduced stress markers and increased cell cycle progression in hepatocytes and the activation of the transcription factor NF-κB in vitro and in vivo. Conclusions Our results demonstrate that the nanomaterial cerium oxide, besides their known antioxidant capacities, can enhance hepatocellular proliferation in experimental models of liver regeneration and drug-induced hepatotoxicity.

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Synthesis and biomedical applications of Cerium oxide nanoparticles – A Review

Biotechnology Reports ◽

10.1016/j.btre.2017.11.008 ◽

2018 ◽

Vol 17 ◽

pp. 1-5 ◽

Cited By ~ 89

Author(s):

S. Rajeshkumar ◽

Poonam Naik

Keyword(s):

Cerium Oxide ◽

Biomedical Applications ◽

Cerium Oxide Nanoparticles ◽

Oxide Nanoparticles

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A Review on Application of Novel Solid Nanostructures in Drug Delivery

Journal of Nano Research ◽

10.4028/www.scientific.net/jnanor.53.22 ◽

2018 ◽

Vol 53 ◽

pp. 22-36 ◽

Cited By ~ 4

Author(s):

Habibollah Faraji ◽

Reza Nedaeinia ◽

Esmaeil Nourmohammadi ◽

Bizan Malaekeh-Nikouei ◽

Hamid Reza Sadeghnia ◽

...

Keyword(s):

Drug Delivery ◽

Drug Therapy ◽

Biomedical Applications ◽

Imaging Biomarkers ◽

Cellular Functions ◽

Scientific Innovation ◽

Wide Range ◽

Targeted Drug

Nanotechnology as a multidisciplinary and scientific innovation plays an important role in numerous biomedical applications, such as molecular imaging, biomarkers and biosensors and also drug delivery. A wide range of studies have been conducted on using of nanoparticles for early diagnosis and targeted drug therapy of various diseases. In fact, the small size, customized surface, upgraded solubility, or multi-functionality of nanoparticles enabled them to interact with complex cellular functions in new ways which opened many doors and created new biomedical applications. These studies demonstrated that nanotechnology vehicles can formulate biological products effectively, and this nano-formulated products with a potent ability against different diseases, were represented to have better biocompatibility, bioaccessibility and efficacy, under in vitro and in vivo conditions.

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