scholarly journals Salt-tolerance mechanism of Staphylococcus aureus

1985 ◽  
Vol 97 (11-12) ◽  
pp. 937-946
Author(s):  
Eiko NAGAMACHI ◽  
Ken-ichi TOMOCHIKA ◽  
Yoshikazu HIRAI ◽  
Takashi MARUYAMA ◽  
Akinobu OKABE ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Salt Tolerance ◽  
Tolerance Mechanism

Growth and enterotoxin B synthesis by Staphylococcus aureus S6 in associative growth with Pseudomonas aeruginosa

1973 ◽  
Vol 19 (10) ◽  
pp. 1197-1201 ◽  
Author(s):  
D. L. Collins-Thompson ◽  
B. Aris ◽  
A. Hurst
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Sodium Chloride ◽  
Salt Tolerance ◽  
Second Growth ◽  
Growth System ◽  
Pure Cultures ◽  
Enterotoxin B ◽  
Membrane Type ◽  
And Staphylococcus Aureus

The interaction of Pseudomonas aeruginosa and Staphylococcus aureus S6 was studied in two systems. In the first system, the two organisms were grown together in a single flask. Growth of P. aeruginosa was unaffected, but growth of S. aureus was modified. After 24 h, 99.9% of the staphylococci population lost their salt tolerance when plated on media containing 7.5% sodium chloride, and enterotoxin B synthesis by S. aureus was diminished. In the second growth system, pure cultures of P. aeruginosa and S. aureus were grown in membrane-type spinner flasks. The growth and salt tolerance of S. aureus was again affected, but to a lesser degree. Cultures of S. aureus from these experiments recovered their salt tolerance in 6 h when transferred to fresh medium.Nutrient deficiency, lack of oxygen, or pigment production by the pseudomonads did not contribute significantly to loss of salt tolerance or inhibition of enterotoxin B synthesis, but a staphylolytic enzyme(s) isolated from P. aeruginosa was shown to be responsible for the loss of these properties.

Repair of salt tolerance and recovery of lost D-alanine and magnesium following sublethal heating of Staphylococcus aureus are independent events

1981 ◽  
Vol 27 (6) ◽  
pp. 627-632 ◽  
Author(s):  
A. Hurst ◽  
A. Hughes
Keyword(s):  
Staphylococcus Aureus ◽  
Salt Tolerance ◽  
Phosphate Buffer ◽  
Synthetic Medium ◽  
Potassium Phosphate ◽  
Complex Media ◽  
Rich Medium ◽  
Potassium Phosphate Buffer ◽  
Heat Damage ◽  
Independent Events

Sublethal heating of Staphylococcus aureus S6 in potassium phosphate buffer caused loss of salt tolerance, D-alanine, and magnesium. During incubation in rich complex media all three of the damaged sites were repaired. Repair occurred more slowly but went to completion in a dilute synthetic medium (DSM), free of D-ala. DSM plus penicillin or D-cycloserine allowed repair of salt tolerance but recovery of normal levels of D-ala or Mg was prevented. When DSM-repaired cells were cultured into fresh rich medium they grew rapidly after a short lag. Cells which had acquired their salt tolerance in DSM plus cycloserine and were D-ala and Mg deficient grew slowly and had a lag of 3 h. We suggest that heat damage has two separate primary targets in S. aureus cells: the membrane, which is manifested by loss of salt tolerance, and a second site, possibly teichoic acids, manifested by loss of D-ala and Mg.

scholarly journals A Computational Systems Biology Study for Understanding Salt Tolerance Mechanism in Rice

PLoS ONE ◽  
2013 ◽  
Vol 8 (6) ◽  
pp. e64929 ◽  
Author(s):  
Juexin Wang ◽  
Liang Chen ◽  
Yan Wang ◽  
Jingfen Zhang ◽  
Yanchun Liang ◽  
...  
Keyword(s):  
Systems Biology ◽  
Salt Tolerance ◽  
Computational Systems Biology ◽  
Tolerance Mechanism ◽  
Computational Systems

Choline-Mediated Lipid Reprogramming as a Dominant Salt Tolerance Mechanism in Grass Species Lacking Glycine Betaine

2020 ◽  
Author(s):  
Kun Zhang ◽  
Weiting Lyu ◽  
Yanli Gao ◽  
Xiaxiang Zhang ◽  
Yan Sun ◽  
...  
Keyword(s):  
Salt Stress ◽  
Salt Tolerance ◽  
Glycine Betaine ◽  
Metabolic Pathways ◽  
Poa Pratensis ◽  
Choline Chloride ◽  
Photochemical Efficiency ◽  
Kentucky Bluegrass ◽  
Grass Species ◽  
Tolerance Mechanism

Abstract Choline, as a precursor of glycine betaine (GB) and phospholipids, is known to play roles in plant tolerance to salt stress, but the downstream metabolic pathways regulated by choline conferring salt tolerance are still unclear for non-GB-accumulating species. The objectives were to examine how choline affects salt tolerance in a non-GB-accumulating grass species and to determine major metabolic pathways of choline regulating salt tolerance involving GB or lipid metabolism. Kentucky bluegrass (Poa pratensis) plants were subjected to salt stress (100 mM NaCl) with or without foliar application of choline chloride (1 mM) in a growth chamber. Choline or GB alone and the combined application increased leaf photochemical efficiency, relative water content and osmotic adjustment and reduced leaf electrolyte leakage. Choline application had no effects on the endogenous GB content and GB synthesis genes did not show responses to choline under nonstress and salt stress conditions. GB was not detected in Kentucky bluegrass leaves. Lipidomic analysis revealed an increase in the content of monogalactosyl diacylglycerol, phosphatidylcholine and phosphatidylethanolamine and a decrease in the phosphatidic acid content by choline application in plants exposed to salt stress. Choline-mediated lipid reprogramming could function as a dominant salt tolerance mechanism in non-GB-accumulating grass species.

scholarly journals Newly Identified Wild Rice Accessions Conferring High Salt Tolerance Might Use a Tissue Tolerance Mechanism in Leaf

2018 ◽  
Vol 9 ◽  
Author(s):  
Manas R. Prusty ◽  
Sung-Ryul Kim ◽  
Ricky Vinarao ◽  
Frederickson Entila ◽  
James Egdane ◽  
...  
Keyword(s):  
Salt Tolerance ◽  
Wild Rice ◽  
High Salt ◽  
Tolerance Mechanism ◽  
High Salt Tolerance

scholarly journals QUORUM QUENCHING POTENTIALS OF PROBIOTIC ENTEROCOCCUS DURANS LAB38 AGAINST METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS

2017 ◽  
Vol 10 (4) ◽  
pp. 445 ◽  
Author(s):  
Sivakumar Natesan ◽  
Seenivasan Boopathi ◽  
Gopal Selvakumar
Keyword(s):  
Staphylococcus Aureus ◽  
Salt Tolerance ◽  
Bile Salt ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Quorum Quenching ◽  
Artemia Salina ◽  
Methicillin Resistant ◽  
Enterococcus Durans ◽  
Azulene Derivative ◽  
Tn5 Mutant

Objective: he focus of this study was to explore the nuance strategy to combat the virulence factors of the pathogens by probiotic Enterococcus durans LAB38Methods: Probiotic attributes was determined by bile salt tolerance (0.5%) and Artemia gnotobiotic assay. Quorum sensing (QS) inhibitory activity of the supernatant and ethyl acetate (EA) extract of LAB38 was evaluated by using the indicator strains, includes Chromobacterium violaceum CV026 (mini-Tn5 mutant of ATCC 31532), methicillin resistant Staphylococcus aureus (MRSA) and Pseudomonas aeruginosa (PA). Reporter strains, Vibrio harveyi BB170 (luxN mutant), BB886 (luxP mutant) and Escherichia coli pSB401 (pACYC184- derived) were used for bioluminescence-based target specificity analysis. GC-MS analysis of EA extract was performed using standard protocol. Results: LAB38 has shown bile salt tolerance and positive probiotic effect towards Artemia salina. In addition, 100 µg/ml EA extract has significantly reduced the violacein production (37 ± 1.4%) in CV026, biofilm formation in MRSA (94 ± 0.9 %) and PA (22 ± 0.08%). Further, 200 µg/ml of EA extract has shown inhibition against both autoinducer-1 and autoinducer-2 mediated QS system. Bioluminescence inhibition is directly proportional to the time of exposure. GC-MS result revealed that bromine, sulphur containing molecule and azulene derivative were found in the EA extract.Conclusion This is the first report on probiotic Enterococcus durans for quorum quenching activity. Hence, the bacterium could be used for future therapeutics application. Keywords: Autoinducer, Biofilm, Methicillin resistant Staphylococcus aureus, Quorum quenching, Probiotics, CV026.

scholarly journals A Golgi-Localized Sodium/Hydrogen Exchanger Positively Regulates Salt Tolerance by Maintaining Higher K+/Na+ Ratio in Soybean

2021 ◽  
Vol 12 ◽  
Author(s):  
Tianjie Sun ◽  
Nan Ma ◽  
Caiqing Wang ◽  
Huifen Fan ◽  
Mengxuan Wang ◽  
...  
Keyword(s):  
Salt Stress ◽  
Salt Tolerance ◽  
Soil Salinization ◽  
Tolerance Mechanism ◽  
Salt Treatment ◽  
Soybean Seedlings ◽  
Yield And Quality ◽  
Sodium Hydrogen ◽  
Sodium Hydrogen Exchanger ◽  
Soybean Plants

Salt stress caused by soil salinization, is one of the main factors that reduce soybean yield and quality. A large number of genes have been found to be involved in the regulation of salt tolerance. In this study, we characterized a soybean sodium/hydrogen exchanger gene GmNHX5 and revealed its functional mechanism involved in the salt tolerance process in soybean. GmNHX5 responded to salt stress at the transcription level in the salt stress-tolerant soybean plants, but not significantly changed in the salt-sensitive ones. GmNHX5 was located in the Golgi apparatus, and distributed in new leaves and vascular, and was induced by salt treatment. Overexpression of GmNHX5 improved the salt tolerance of hairy roots induced by soybean cotyledons, while the opposite was observed when GmNHX5 was knockout by CRISPR/Cas9. Soybean seedlings overexpressing GmNHX5 also showed an increased expression of GmSOS1, GmSKOR, and GmHKT1, higher K+/Na+ ratio, and higher viability when exposed to salt stress. Our findings provide an effective candidate gene for the cultivation of salt-tolerant germplasm resources and new clues for further understanding of the salt-tolerance mechanism in plants.

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