An usual approach to treatment of a case of multidrug resistance Pseudomonas aeruginosa peritonitis: parenteral and intraperitoneal aminoglycosides and parenteral colistin

Infections caused by <em>Pseudomonas aeruginosa </em>are becoming more common and increasingly more difficult to treat due to the continued development of drug resistance. While sensitivity to colistin (polymyxin E) is well known, it is frequently avoided due to concerns of nephrotoxicity. Reported here is a case of a multi-drug resistance pseudomonal typhlitis, bacteremia and pleural cavity infection that required significant intensive care, and serial abdominal washouts. Intra-peritoneal tobramycin in combination with broad-spectrum intravenous antibiotics including colistin were used. Several instillations of tobramycin into the abdominal cavity along with concomitant IV administration of colistin, ceftazidime and tobramycin and<em> per os</em> colistin, tobramycin and nystatin resulted in the clearance of the pseudomonal infection without any evidence of toxicity from the treatment. Intra-abdominal tobramycin with parenteral colistin therapy can be used in complicated clinical settings with appropriate nephroprotection.

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Establishment and multi drug resistance evolution of ST235 Pseudomonas aeruginosa strains in the intensive care unit of a Colombian hospital

Research in Microbiology ◽

10.1016/j.resmic.2014.10.011 ◽

2014 ◽

Vol 165 (10) ◽

pp. 852-856 ◽

Cited By ~ 6

Author(s):

Elena Martinez ◽

Javier Escobar Pérez ◽

Francisco Buelvas ◽

Catalina Tovar ◽

Natasha Vanegas ◽

...

Keyword(s):

Intensive Care Unit ◽

Pseudomonas Aeruginosa ◽

Drug Resistance ◽

Intensive Care ◽

Multi Drug Resistance ◽

Resistance Evolution

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Central line associated blood stream infectious cause by multi drug resistance coagulaso-negative Staphylococci in newborns from neonatial intensive care units in Poland

BMC Proceedings ◽

10.1186/1753-6561-5-s6-p205 ◽

2011 ◽

Vol 5 (Suppl 6) ◽

pp. P205

Author(s):

M Brzychczy-Wloch ◽

J Wojkowska-Mach ◽

M Borszewska-Kornacka ◽

M Sulik ◽

E Gulczynska ◽

...

Keyword(s):

Drug Resistance ◽

Intensive Care ◽

Intensive Care Units ◽

Blood Stream ◽

Central Line ◽

Multi Drug Resistance

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An α-Lipoic acid derivative, and anti-ROS agent, prevents the acquisition of multi-drug resistance in clinical isolates of Pseudomonas aeruginosa

Journal of Infection and Chemotherapy ◽

10.1016/j.jiac.2018.10.003 ◽

2019 ◽

Vol 25 (1) ◽

pp. 28-33 ◽

Cited By ~ 1

Author(s):

Sachiko Hayakawa ◽

Masato Kawamura ◽

Takumi Sato ◽

Taizou Hirano ◽

Toshiaki Kikuchi ◽

...

Keyword(s):

Pseudomonas Aeruginosa ◽

Drug Resistance ◽

Acid Derivative ◽

Lipoic Acid ◽

Clinical Isolates ◽

Multi Drug Resistance

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Berberine Improves Chemo-Sensitivity to Cisplatin by Enhancing Cell Apoptosis and Repressing PI3K/AKT/mTOR Signaling Pathway in Gastric Cancer

Frontiers in Pharmacology ◽

10.3389/fphar.2020.616251 ◽

2020 ◽

Vol 11 ◽

Author(s):

Yingying Kou ◽

Bending Tong ◽

Weiqing Wu ◽

Xiangqing Liao ◽

Min Zhao

Keyword(s):

Gastric Cancer ◽

Drug Resistance ◽

Multidrug Resistance ◽

Cell Viability ◽

Cancer Cells ◽

Cell Apoptosis ◽

Mtor Signaling ◽

Multi Drug Resistance ◽

Gastric Cancer Cells

Gastric cancer is one of the most common malignancies ranks as the second leading cause of cancer-related mortality in the world. Cisplatin (DDP) is commonly used for gastric cancer treatment, whereas recurrence and metastasis are common because of intrinsic and acquired DDP-resistance. The aim of this study is to examine the effects of berberine on the DDP-resistance in gastric cancer and explore the underling mechanisms. In this study, we established the DDP-resistant gastric cancer cells, where the IC50 values of DDP in the BGC-823/DDP and SGC-7901/DDP were significantly higher than that in the corresponding parental cells. Berberine could concentration-dependently inhibited the cell viability of BGC-823 and SGC-7901 cells; while the inhibitory effects of berberine on the cell viability were largely attenuated in the DDP-resistant cells. Berberine pre-treatment significantly sensitized BGC-823/DDP and SGC-7901/DDP cells to DDP. Furthermore, berberine treatment concentration-dependently down-regulated the multidrug resistance-associated protein 1 and multi-drug resistance-1 protein levels in the BGC-823/DDP and SGC7901/DDP cells. Interestingly, the cell apoptosis of BGC-823/DDP and SGC-7901/DDP cells was significantly enhanced by co-treatment with berberine and DDP. The results from animals also showed that berberine treatment sensitized SGC-7901/DDP cells to DDP in vivo. Mechanistically, berberine significantly suppressed the PI3K/AKT/mTOR in the BGC-823/DDP and SGC-7901/DDP cells treated with DDP. In conclusion, we observed that berberine sensitizes gastric cancer cells to DDP. Further mechanistic findings suggested that berberine-mediated DDP-sensitivity may be associated with reduced expression of drug transporters (multi-drug resistance-1 and multidrug resistance-associated protein 1), enhanced apoptosis and repressed PI3K/AKT/mTOR signaling.

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The roles of four multi-drug resistance proteins in hepatocellular carcinoma multidrug resistance

Journal of Huazhong University of Science and Technology [Medical Sciences] ◽

10.1007/s11596-007-0217-8 ◽

2007 ◽

Vol 27 (2) ◽

pp. 173-175 ◽

Cited By ~ 22

Author(s):

Gaopeng Li ◽

Xiaoping Chen ◽

Qi Wang ◽

Zongquan Xu ◽

Wanguang Zhang ◽

...

Keyword(s):

Hepatocellular Carcinoma ◽

Drug Resistance ◽

Multidrug Resistance ◽

Multi Drug Resistance ◽

Resistance Proteins

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The bactericidal activity of Magnetic water on Multi Drug Resistance [ MDR] Pseudomonas aeruginosa Resistance Colistin from clinical and environmental sources and effect on Biofilm

10.24897/acn.64.68.31 ◽

2017 ◽

Keyword(s):

Pseudomonas Aeruginosa ◽

Drug Resistance ◽

Bactericidal Activity ◽

Multi Drug Resistance ◽

Magnetic Water

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Multidrug resistance and high prevalence of class 1 integrons in Escherichia coli isolated from waters and vegetables in Nsukka and Enugu, Nigeria

10.1101/2020.01.06.895748 ◽

2020 ◽

Author(s):

Chinyere B. Chigor ◽

Ini-Abasi I. Ibangha ◽

Nkechinyere O. Nweze ◽

Chizoba A. Ozochi ◽

Valentino C. Onuora ◽

...

Keyword(s):

Escherichia Coli ◽

Drug Resistance ◽

Multidrug Resistance ◽

Treatment Plant ◽

Treated Wastewater ◽

Multi Drug Resistance ◽

E Coli ◽

Class 1 Integrons ◽

High Prevalence ◽

Class 1

AbstractIn spite of treated wastewater presenting itself as an attractive alternative to scarce quality water in the developing countries, the associated contamination of fresh produce by irrigation waters leading to outbreak of foodborne illnesses is on the rise. Horizontal transfer of integrons play important role in the spread and maintenance of antimicrobial resistance among strains of Escherichia coli. This study assessed the effluents from the University of Nigeria, Nsukka Wastewater Treatment Plant (UNN-WWTP) as well as vegetables irrigated with the effluent, and vegetables sold in selected markets from Nsukka and Enugu cities for the presence of E. coli and determined the prevalence integrons in multidrug-resistant isolates. Isolation of E. coli was done using eosin methylene blue agar and isolates subjected to Gram staining for identification of presumptive colonies. Confirmation of E. coli was achieved by polymerase chain reaction (PCR) technique, targeting beta-glucuronidase (uidA). Resistance to antibiotics was determined using the Bauer-Kirby disk diffusion assay and the Clinical and Laboratory Standard Institute criteria. Integrons were detected by multiplex PCR using primers specific for class 1 and 2 integrons. A total of 178 E. coli isolates were obtained from WWTP effluent (41), and vegetables from greenhouse (46), farms (55) and market (36). Multi-drug resistance was detected in all the isolates, ranging from five-drug resistance in a single isolate to 16-drug resistance patterns in two different isolates. Of the total isolates, class 1 integrons were abundantly detected in 175 (98.3%) and class 2 in 5 (2.8%). All the class 2 integrons were found in isolates that were positive for class 1. The high detection of E. coli in the studied effluent and vegetables pose potential public health hazards heightened by observed multidrug resistance in all the isolates and the high prevalence of class 1 integron. It is concluded that the vegetable samples are significant reservoirs for potentially pathogenic E. coli. Therefore, vegetable irrigation farming with unsafe water should be discontinued, while appropriate improvement strategies to ensure compliance should be facilitated without further delay.

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Plasmodium falciparum Multidrug Resistance Proteins (pfMRPs)

Frontiers in Pharmacology ◽

10.3389/fphar.2021.759422 ◽

2021 ◽

Vol 12 ◽

Author(s):

José Pedro Gil ◽

Cláudia Fançony

Keyword(s):

Drug Resistance ◽

Plasmodium Falciparum ◽

Multidrug Resistance ◽

Multi Drug Resistance ◽

Multidrug Resistance Proteins ◽

Resistance Proteins ◽

Diverse Groups ◽

Endogenous Substances

The capacity of the lethal Plasmodium falciparum parasite to develop resistance against anti-malarial drugs represents a central challenge in the global control and elimination of malaria. Historically, the action of drug transporters is known to play a pivotal role in the capacity of the parasite to evade drug action. MRPs (Multidrug Resistance Protein) are known in many phylogenetically diverse groups to be related to drug resistance by being able to handle a large range of substrates, including important endogenous substances as glutathione and its conjugates. P. falciparum MRPs are associated with in vivo and in vitro altered drug response, and might be important factors for the development of multi-drug resistance phenotypes, a latent possibility in the present, and future, combination therapy environment. Information on P. falciparum MRPs is scattered in the literature, with no specialized review available. We herein address this issue by reviewing the present state of knowledge.

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Identification of floR Variants Associated With a Novel Tn4371-Like Integrative and Conjugative Element in Clinical Pseudomonas aeruginosa Isolates

Frontiers in Cellular and Infection Microbiology ◽

10.3389/fcimb.2021.685068 ◽

2021 ◽

Vol 11 ◽

Author(s):

Changrui Qian ◽

Hongmao Liu ◽

Jiawei Cao ◽

Yongan Ji ◽

Wei Lu ◽

...

Keyword(s):

Pseudomonas Aeruginosa ◽

Drug Resistance ◽

Respiratory Diseases ◽

Essential Role ◽

Multi Drug Resistance ◽

Clinical Samples ◽

Integrative And Conjugative Elements ◽

Intestinal Infections ◽

Clinical Pathogens ◽

Integrative And Conjugative Element

Florfenicol is widely used to control respiratory diseases and intestinal infections in food animals. However, there are increasing reports about florfenicol resistance of various clinical pathogens. floR is a key resistance gene that mediates resistance to florfenicol and could spread among different bacteria. Here, we investigated the prevalence of floR in 430 Pseudomonas aeruginosa isolates from human clinical samples and identified three types of floR genes (designated floR, floR-T1 and floR-T2) in these isolates, with floR-T1 the most prevalent (5.3%, 23/430). FloR-T2 was a novel floR variant identified in this study, and exhibited less identity with other FloR proteins than FloRv. Moreover, floR-T1 and floR-T2 identified in P. aeruginosa strain TL1285 were functionally active and located on multi-drug resistance region of a novel incomplete Tn4371-like integrative and conjugative elements (ICE) in the chromosome. The expression of the two floR variants could be induced by florfenicol or chloramphenicol. These results indicated that the two floR variants played an essential role in the host’s resistance to amphenicol and the spreading of these floR variants might be related with the Tn4371 family ICE.

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