scholarly journals DNA barcoding and nutritional analysis as a tool for promoting the market of inland fish species

2021 ◽  
Vol 10 (3) ◽  
Author(s):  
Iolanda Venuti ◽  
Marina Ceruso ◽  
Giuseppe Palma ◽  
Giorgio Smaldone ◽  
Tiziana Pepe
Keyword(s):  
Fatty Acids ◽  
Dna Barcoding ◽  
Fish Species ◽  
Cyprinus Carpio ◽  
Micropterus Salmoides ◽  
Central Italy ◽  
Anguilla Anguilla ◽  
Coi Gene ◽  
Local Market ◽  
Market Demand

The increasing world market demand for seafood requires an expansion of product categories available to consumers. Inland fish are usually considered having unmarked taste and are less appreciated by consumers; thus, they have low commercial value. Therefore, the marketing of the lake’s fresh and processed fish is limited to the local market and consumers are currently uninformed and mistrustful about these species. In this study, six different fish species were caught in the Fondi lake (Lazio, central Italy): Anguilla anguilla, Tinca tinca, Carassius gibelio, Cyprinus carpio, Micropterus salmoides, Chelon ramada. All the samples were subjected to nutritional and DNA barcoding analysis. Moisture, protein, fat, carbohydrates, ash, and sodium content were measured. As regards the fatty acids profile, the most abundant were MUFAs with the highest value in Anguilla anguilla (45.97%). Oleic acid (C18: 1 n9 cis) was particularly high in Cyprinus carpio (55.46%). The fraction of polyunsaturated fatty acids (PUFA) revealed a higher DHA content (C22: 6 n3) in Anguilla anguilla than the other species (>12 %) while Chelon ramada presented both higher EPA content (C 20: 5 n3) and total fraction of omega 3 PUFAs. Concerning molecular analysis, a 655 bp fragment of cytochrome C oxidase subunit I (COI) gene was successfully used for the identification at the species level using both BOLD and BLAST public databases. The present study gives the basis for improving the knowledge and promoting inland fish’ market and traceability along the supply chain.

scholarly journals DNA Barcoding: A Study of Guppy Fish (Poecilia reticulata) in East Java, Indonesia

2019 ◽  
Vol 11 (2) ◽  
pp. 272-278
Author(s):  
Sucipto Hariyanto ◽  
Hasan Adro’i ◽  
Mahrus Ali ◽  
Bambang Irawan
Keyword(s):  
Dna Barcoding ◽  
Fish Species ◽  
Dna Sequences ◽  
Poecilia Reticulata ◽  
Phylogenetic Reconstruction ◽  
Coi Gene ◽  
West Java ◽  
Advance Research ◽  
Phylogeny And Evolution ◽  
Guppy Fish

Poecilia reticulata is a freshwater fish from the northeastern part of South America and spread widely to various countries in Asia and other continents. However, research about P. reticulate is limited even though it is a well-known fish species in Indonesia. The purpose of study was to identify the fish species of P. reticulata through DNA barcoding using the COI gene to determine the phylogenetic relationships among fish populations in East Java, Indonesia. In a present study, there were eight samples of P. reticulata from four different freshwater locations in East Java. Extraction, amplification, and sequencing of DNA samples were conducted to obtain the genetic data and construct a phylogenetic tree based on DNA sequences. The COI gene is the most popular markers to study genetic populations and phylogeography among the animal kingdom. Our phylogenetic reconstruction showed a clear that there were two groups of P. reticulata. The first group was obtain through species from East Java, Sukabumi, West Java (KU692776.1), Dominican Republic, Pandeglang, Banten and Myanmar. The second group was P. reticulata from southern Africa, Brazil, and Sukabumi, West Java (KU692775.1). The result of this study indicate that the guppy fish in East Java identic with P. reticulata from West Java (KU692776.1), which a widely used in classification based on evolutionary relationships. The findings of this study have important implication for the development of advance research about adaptation, phylogeny, and evolution of fish, especially of guppy fish.

scholarly journals Fatty Acid Composition of the Muscle Lipids of Five Fish Species in Işıklı and Karacaören Dam Lake, Turkey

2014 ◽  
Vol 2014 ◽  
pp. 1-5 ◽  
Author(s):  
Ozcan Baris Citil ◽  
Leyla Kalyoncu ◽  
Oguzhan Kahraman
Keyword(s):  
Fatty Acids ◽  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Acid Composition ◽  
Fish Species ◽  
Cyprinus Carpio ◽  
Carassius Carassius ◽  
Tinca Tinca ◽  
Scardinius Erythrophthalmus ◽  
Muscle Lipids

Total fatty acid composition of muscle lipids in some fish species (Cyprinus carpio(Işıklı Dam Lake),Tinca tinca(Işıklı Dam Lake),Scardinius erythrophthalmus(Işıklı Dam Lake),Cyprinus carpio(Karacaören Dam Lake), andCarassius carassius(Karacaören Dam Lake)) was determined by gas chromatography. Polyunsaturated fatty acids (PUFAs) ofCyprinus carpio(Işıklı Dam Lake) were found higher than PUFA of other species. Palmitic acid was the highest saturated fatty acid (SFA) inTinca tinca(24.64%). Oleic acid was the highest monounsaturated fatty acid (MUFAs) inCyprinus carpio(Işıklı Dam Lake) (19.25%). The most abundant polyunsaturated fatty acid inScardinius erythrophthalmuswas docosahexaenoic acid (DHA) (17.94%). Totalω3 fatty acid composition was higher than the totalω6 fatty acids ofCyprinus carpioin both dam lakes.ω3/ω6 rates inCyprinus carpio(Işıklı Dam Lake),Tinca tinca, Scardinius erythrophthalmus, Cyprinus carpio(Karacaören), andCarassius carassiuswere 2.12, 1.19, 2.15, 2.87, and 2.82, respectively.

scholarly journals Use of DNA barcode in the identification of catfishes (Siluriformes: Ariidae) from Malaysia

2017 ◽  
Vol 18 (4) ◽  
pp. 1358-1366
Author(s):  
MOHD LUTFI ABDULLAH ◽  
SITI AZIZAH MOHD NOR ◽  
DARLINA MD. NAIM
Keyword(s):  
Dna Barcoding ◽  
Fish Species ◽  
Incomplete Lineage Sorting ◽  
Molecular Taxonomy ◽  
Dna Barcode ◽  
Coi Gene ◽  
Lineage Sorting ◽  
Taxonomic Studies ◽  
Mitochondrial Cytochrome Oxidase ◽  
Blast Result

Abdullah ML, Nor SAM, Naim DMd. 2017. Use of DNA barcode in the identification of catfishes (Siluriformes: Ariidae) from Malaysia. Biodiversitas 18: 1358-1366. The genus Ariidae contains many valuable fish species threatened by overfishing, but knowledge on distribution and threats is still limited due to taxonomic ambiguities. The aim of this study was to apply DNA barcoding techniques to establish a resource of DNA for identification of Ariidae species in Malaysia. A 621 bp of mitochondrial cytochrome oxidase subunit I (COI) gene was utilized to resolve phylogenetic relationships and molecular taxonomy of eight presumed Malaysian Ariid species. We found the monophyly of most species was well established with a mean Kimura-2 parameter (K2P) interspecies distance of 9.6% except for two species, Arius venosus, and Nemapteryx caelata that have very low interspecies genetic distance. The BLAST result shows only two species matched the presumably eight identified fish species. Such discrepancies could arise as a result of misidentifications or errors in GenBank database input, hybridization or incomplete lineage sorting. We suggest the use of DNA barcoding is integrated into the workflow during taxonomic studies as it could significantly increase knowledge about species distributions.

Significance of Detritus or Detritus-Associated Invertebrates to Fish Production in a New Impoundment

1975 ◽  
Vol 32 (10) ◽  
pp. 1799-1804 ◽  
Author(s):  
Donald S. Cherry ◽  
Rufus K. Guthrie
Keyword(s):  
Relative Abundance ◽  
Fish Species ◽  
Cyprinus Carpio ◽  
Micropterus Salmoides ◽  
Stomach Contents ◽  
Frequency Of Occurrence ◽  
Fish Production ◽  
Animal Food ◽  
Food Items ◽  
Carp Cyprinus Carpio

During early stages of succession in a new impoundment, fish species that could assimilate the readily available food resources, i.e. carp (Cyprinus carpio) and flat bullhead (Ictalurus platycephalus) were initially favored in numbers and weight. Detrital feeders or omnivores represented by carp and the flat bullhead comprised a majority of the numbers (56%) and weight (65%) of the 25 species captured during a 2-yr period. Numbers of the major fish groups captured decreased from 1971 to 1972, while the weight per species increased.On a volumetric basis, detritus and detritus-associated invertebrates (coleopterans and dipterans), were the major food items consumed by carp and catfish. Relative abundance of animal food items, in terms of frequency of occurrence and percentage of stomach contents, was greater at warmer water temperatures. In comparison to other species, catfish contained the greatest diversity of food items during warmer months and consumed larger quantities of detritus in the winter. Fish groups that constituted a smaller biomass, such as centrarchids, were generally insectivorous or fed upon cladocerans and copepods, while adult basses (Micropterus salmoides and M. coosae) and chain pickerel (Esox niger) were piscivorous.

scholarly journals DNA BARCODING OF FRESHWATER FISH SPECIES OF MANNA RIVER (BENGKULU) AND SEMANKA RIVER (LAMPUNG)

2013 ◽  
Vol 19 (1) ◽  
pp. 9 ◽  
Author(s):  
Arif Wibowo ◽  
Achmad Farajalah ◽  
Husnah Husnah
Keyword(s):  
Freshwater Fish ◽  
Dna Barcoding ◽  
Fish Species ◽  
Single Species ◽  
Interspecific Variation ◽  
Coi Gene ◽  
Reciprocal Monophyly ◽  
Freshwater Fish Species ◽  
Interspecific Comparisons ◽  
Two Parameters

The identification of fish species is challenging. DNA barcoding provides new perspective in ecology and systematics of fishes. In this study, we assess intraspecific and interspecific genetic divergence among several freshwater fish species from Manna River of Bengkulu Province and Semanka River of Lampung Province, using data from mitochondrial cytochrome c oxidase subunit I (COI) gene. The results show that a total of six freshwater fish species identified in this study exhibited high levels of interspecific variation based on 51 analyzed sequences. Almost all species corresponded to a single species, cohesive array of barcode sequences distinct from other species. Based on Kimura Two Parameters (K2P) within species, mean pairwise sequence distances comparisons range from 0.3 to 6.3%, while interspecific comparisons were much higher range between 1.7 to 29.4%, except species Tor macrolepis and Tor putitora showed high levels of genetic similarities and no reciprocal monophyly. Using this method will obviously allow the identification eggs, larvae, fillets and fins of freshwater fish.

scholarly journals DNA Barcoding for Selected Mangrove-Based Estuary Fishes from Way Kambas National Park, Lampung Province, Indonesia

2021 ◽  
Vol 11 (2) ◽  
pp. 151-160
Author(s):  
Yanti Ariyanti ◽  
◽  
Ika Rini ◽  
Indah Oktaviani ◽  
Sovia Leksikowati ◽  
...  
Keyword(s):  
Dna Barcoding ◽  
Fish Species ◽  
National Park ◽  
Conservation Status ◽  
Genetic Distances ◽  
Coi Gene ◽  
Species Discovery ◽  
Mitochondrial Coi ◽  
Identification Of Species ◽  
Way Kambas National Park

Over the past decade, DNA barcoding has provided new insight into fish ecology and biosystematics and led to new species' discovery. DNA barcoding is a method for the recognition and identification of species using short, standardised DNA fragments. The correct taxonomic identification of species is critical for the assessment and monitoring of biodiversity. This study applied DNA barcoding techniques to identify selected fish species from a mangrove-based estuary in Way Kambas National Park, Lampung Province, Indonesia. The gene encoding cytochrome c oxidase subunit I (COI) was amplified and bi-directionally sequenced from 22 specimens. The resulting 680 base pairs (bp) sequence was used to identify species, obtain phylogenetic information, and analyse genetic distances. A neighbour-joining tree was constructed based on the mitochondrial COI gene using the Kimura two-parameter model. This study also exhibits conservation status for those identified species. Our findings will facilitate future studies of fish species diversity in mangrove estuary-based ecosystems and provide preliminary data in policymaking in conservation areas such as National Park.

scholarly journals Comparative study of the fatty acid composition of light/dark mixture musculature of five freshwater fish from the region of Dezful, Iran

2021 ◽  
Vol 4 (1) ◽  
pp. 57
Author(s):  
Seyed Mehrshad Gaffari ◽  
Zahra Khoshnood
Keyword(s):  
Fatty Acids ◽  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Freshwater Fish ◽  
Acid Composition ◽  
Fish Species ◽  
Cyprinus Carpio ◽  
Omega 3 ◽  
Fish Samples ◽  
Squalius Cephalus

Fatty acid composition of fish is one of the key factors for the evaluation of fish nutritional value and it could be different based on fish species, habitat, nutrition and etc. The aim of the present study was to evaluate the fatty acid composition of five commercially important freshwater fish species taken from fisheries sites at Dez River, Iran. The fish samples included Heckel’s Orontes barbell, Luciobarbus pectoralis (Heckel, 1843), Chub, Squalius cephalus (Linnaeus, 1758), Common carp, Cyprinus carpio (Linnaeus, 1758), Brond-snout, Chondrostoma regium (Heckel, 1843) and Longspine scraper, Capoeta trutta (Heckel, 1843). The determination of fatty acid contents of the fish fillets were conducted by means of Gas chromatography. Results showed that Luciobarbus pectoralis fillets had the highest amount of fatty acids (43.49%). Cyprinus carpio had the highest amount of saturated fat (SAF) (29.43%) whereas the Luciobarbus pectoralis fillets had the highest MUFA (Monounsaturated fatty acid) (18.51%). The Squalius cephalus fillets possessed the highest amount of PUFA (polyunsaturated fatty acid) (1.87%). The Chondrostoma regium fillets revealed the highest n-6 (1.1%) while the Squalius cephalus fillets had the highest omega 3 (1.87%). The results of the present study showed that these freshwater fish are good sources of n-3 fatty acids, especially EPA and DHA, and could be considered as a valuable nutritional source.

scholarly journals The Analysis of Morphological and Genetic Characteristics of Yellowstripe Scad from Muara Baru Modern Fish Market in North Jakarta

2020 ◽  
Vol 12 (2) ◽  
pp. 308
Author(s):  
Rahmat Sawalman ◽  
Hawis Madduppa
Keyword(s):  
Dna Barcoding ◽  
Molecular Analysis ◽  
Fish Species ◽  
Morphological Analysis ◽  
Coi Gene ◽  
Cytochrome Oxidase Subunit ◽  
Mitochondrial Cytochrome Oxidase Subunit ◽  
Fish Market ◽  
Commercially Important ◽  
Mitochondrial Cytochrome Oxidase

HighlightIdentification and morphological analysis of Selaroides leptolepis fish.Molecular analysis using the DNA Barcoding method with the COI gene (mitochondrial cytochrome oxidase subunit I).The number of base pairs in S. leptolepis from the sequencing results was 675bp.The morphological and molecular similarities of Selaroides leptolepis fishAbstractYellowstripe scad is one of the commercially important fish from the Carangidae family, which is marketed at Muara Baru Modern Fish Market, North Jakarta. In- formation regarding the presence of Selaroides leptolepis fish in the waters allows for effective conservation, and management of marine resources. A morphological identification of fish species is still considered inaccurate, so the molecular anal- ysis is necessary. This study aims to identify commercially important fish species deriving from Muara Baru Modern Fish Market, North Jakarta, employing mor- phological, and molecular analysis. A total of 30 specimens were collected from the field. The morphological analysis utilized a visual identification method, and morphometric measurement, while molecular analysis with DNA barcoding em- ployed the mitochondrial cytochrome oxidase subunit I (COI) gene. The results of the morphological analysis indicated that the fish species were yellowstripe scad (S. leptolepis). It also followed the results of molecular analysis of DNA barcoding that the fish was a S. leptolepis species. Therefore, the combination of morphological and genetic analysis has succeeded in identifying the fish species of S. leptolepis.

Efficacy of DNA barcoding for the identification of larval fish species in the Upper and Middle Ing River, Thailand

Gene Reports ◽  
2021 ◽  
Vol 23 ◽  
pp. 101057
Author(s):  
Dutrudi Panprommin ◽  
Kanyanat Soontornprasit ◽  
Siriluck Tuncharoen ◽  
Niti Iamchuen
Keyword(s):  
Dna Barcoding ◽  
Fish Species ◽  
Larval Fish
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