scholarly journals Screening and partial purification of photoprotective pigment scytonemin from cyanobacterial crusts dwelling on the historical monuments in and around Varanasi, India

2017 ◽  
Vol 8 (1) ◽  
Author(s):  
Jainendra Pathak ◽  
Arun S. Sonker ◽  
R. Richa ◽  
R. Rajneesh ◽  
Vinod K. Kannaujiya ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Solar Radiation ◽  
Spectroscopic Data ◽  
High Performance ◽  
Hplc Analysis ◽  
Partial Purification ◽  
Cyanobacterial Species ◽  
Historical Monuments ◽  
Vis Spectroscopy ◽  
Almost All

In the present investigation, biological crusts from the surface of eight historical monuments of Varanasi, India, were examined for the presence of scytonemin (a cyanobacterial photoprotective pigment) containing cyanobacterial species. Lyngbya sp. and Scytonema sp. were the dominant cyanobacteria present in all crust samples. The absorption spectroscopic data of chlorophyll, carotenoids and scytonemin showed that scytonemin was more abundant than the carotene and chlorophyll in all the crusts. Identification of these compounds was done using UV-Vis spectroscopy and High Performance Liquid Chromatography (HPLC) analysis. HPLC analysis revealed the presence of scytonemin in seven out of eight samples and peaks of scytonemin with retention time ranging from 1.4-1.9 min with corresponding absorbance maxima at 386, 300 and 252±2 nm. As per our knowledge this is the first report of its kind from monuments of Varanasi. From this study, it can be concluded that synthesis of photoprotective compounds like scytonemin and its derivatives counteract the damaging effects of solar radiation which enable cyanobacteria to colonize and inhabit almost all kinds of habitats, including extreme lithic habitats, such as rocks and walls of monuments which face prolonged high intensity solar radiation.

HPLC Analysis of Oxytetracycline Residues in Honey

1986 ◽  
Vol 49 (5) ◽  
pp. 383-388 ◽  
Author(s):  
PETER SPORNS ◽  
SUET KWAN ◽  
LAWRENCE A. ROTH
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Aqueous Solutions ◽  
High Performance ◽  
Hplc Analysis ◽  
Extraction Procedure ◽  
Hplc Method ◽  
Limits Of Detection ◽  
Ph Values ◽  
Double Extraction

Oxytetracycline (OTC), also known commercially as Terramycin, was determined to be more stable in honey than in buffered aqueous solutions at similar pH values and temperatures. A rapid high performance liquid chromatography (HPLC) method was developed to detect and quantitate OTC using a 1:1 dilution (wt/wt) of honey samples in water. Using 355 nm as the wavelength of detection, amounts as low as 0.5 μg/ml could be detected in the above solution. The limits of detection were lowered considerably by a double extraction procedure.

A study of the decomposition behaviour of 12-tungstophosphate heteropolyacid in solution

2003 ◽  
Vol 81 (10) ◽  
pp. 1044-1050 ◽  
Author(s):  
Zhirong Zhu ◽  
Ruan Tain ◽  
Colin Rhodes
Keyword(s):  
Aqueous Solution ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Potentiometric Titration ◽  
Inductively Coupled Plasma ◽  
High Performance ◽  
Atomic Emission ◽  
Inductively Coupled ◽  
Vis Spectroscopy ◽  
Decomposition Behaviour

In this paper, the decomposition of H3PW12O40 in aqueous solution or in mixed solutions of water–ethanol or water–acetone is investigated by potentiometric titration and 31P NMR. Identification of the products from H3PW12O40 decomposition over a pH range of 1–12 was achieved using preparation high performance liquid chromatography (Pre-HPLC) combined with IR, UV–vis spectroscopy, and inductively coupled plasma atomic emission spectroscopy (ICP). It is found that H3PW12O40 in aqueous solution decomposes in a stepwise fashion with increasing pH, with the following solution compositions: [PW12O40]3– (at pH ~ 1) [Formula: see text] [PW12O40]3– + [P2W21O71]6– + [PW11O39]7– (at pH 2.2) [Formula: see text] [PW12O40]3– + [P2W21O71]6– + [PW11O39]7– + [P2W18O62]6– + [P2W19O67]10– (at pH 3.5) [Formula: see text] [P2W21O71]6– + [PW11O39]7– + [P2W18O62]6– (at pH 5.4) [Formula: see text] [PW9O34]9– (at pH 7.3) [Formula: see text] PO43– + WO42– (pH > 8.3). In the first stages at pH < 8, H3PW12O40 decomposes partially with removal of W=O units. In the second stage at pH > 8, tungstophosphoric completely decomposes to PO43–. In contrast, the decomposition of H3PW12O40 is reduced, or the stability of the [PW12O40]3– anion is enhanced, in ethanol–water or acetone solution at pH < 8. Key words: 12-tungstophosphate heteropolyacid, decomposition behaviour, potentiometric titration, 31P NMR, preparation high performance liquid chromatography.

Impurities from polypropylene microcentrifuge tubes as a potential source of interference in simultaneous analysis of multiple lipid-soluble antioxidants by HPLC with electrochemical detection

2004 ◽  
Vol 42 (4) ◽  
Author(s):  
Hsiu-Chuan Yen ◽  
Yi-Tzu Hsu
Keyword(s):  
High Performance Liquid Chromatography ◽  
Electrochemical Detection ◽  
High Performance ◽  
Hplc Analysis ◽  
Coenzyme Q ◽  
Retinyl Palmitate ◽  
Plasma Samples ◽  
Potential Source ◽  
Effect Of Impurities ◽  
Β Carotene

AbstractHigh-performance liquid chromatography (HPLC) with electrochemical detection was used to analyze lipidsoluble antioxidants and micronutrients in plasma. Small amounts of plasma samples are often extracted in polypropylene (PP) microcentrifuge tubes before HPLC analysis due to its convenience. We therefore investigated the effect of impurities released from different PP tubes during extraction on the electrochemical detection of retinol, lutein, α-tocopherol, γ-tocopherol, retinyl palmitate, β-carotene and total coenzyme Q

ANTHOCYANIN, AGLYCONE AND AGLYCONE-SUGAR CONTENT OF FRUITS OF TEMPERATE NORTH AMERICAN SPECIES OF Gaylussacia (ERICACEAE)

1988 ◽  
Vol 68 (1) ◽  
pp. 247-253 ◽  
Author(s):  
J. R. BALLINGTON ◽  
W. E. BALLINGER ◽  
E. P. MANESS
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
North American ◽  
High Performance ◽  
Hplc Analysis ◽  
Sugar Content ◽  
North American Species ◽  
The Other ◽  
Phylogenetic Implications

HPLC analysis of the true huckleberry species Gaylussacia baccata, G. dumosa, G. frondosa, G. mosieri, and G. ursina identified the 3-monoarabinosides, 3-monogalactosides, and 3-monoglucosides of cyanidin, delphinidin, malvidin, peonidin, and petunidin. Gaylussacia brachycera contained all anthocyanins, except peonidin-3-arabinoside. Gaylussacia brachycera differed from other species in percent delphinidin-3-arabinoside. It was higher than the other species in percent of the aglycone delphinidin and lower in cyanidin, and also higher in percent of the sugar arabinose. There were no detectable differences among the other species for anthocyanins, aglycones, or aglycone-sugars. The phylogenetic implications of the similarities among species of Gaylussacia and Vaccinium in anthocyanins, aglycones, and aglycone-sugars of the fruit were discussed.Key words: High-performance liquid chromatography, huckleberries, blueberries, chemotaxonomy, taxonomy, biosystematics

Effects of Baicalein on Prostanoid Generation from the Lung and Contractile Responses of the Trachea in Guinea Pig

1997 ◽  
Vol 25 (01) ◽  
pp. 37-50 ◽  
Author(s):  
Koji Miyamoto ◽  
Takeshi Katsuragi ◽  
Parhat Abdu ◽  
Tatsuo Furukawa
Keyword(s):  
High Performance Liquid Chromatography ◽  
Smooth Muscle ◽  
Liquid Chromatography ◽  
Guinea Pig ◽  
High Performance ◽  
Hplc Analysis ◽  
Antigen Challenge ◽  
Cyclooxygenase Inhibitor ◽  
Prostaglandin F ◽  
Stimulation Of

Effects of baicalein on release of slow reacting substance of anaphylaxis (SRS-A) or leukotriene (L T) from the sensitized guinea pig lung after antigen challenge and tonus of guinea pig tracheal muscles were studied. Baicalein inhibited release of SRS-A from sensitized guinea pig lung after antigen challenge. High-performance liquid chromatography (HPLC) analysis revealed that released SRS-A consisted of LTC 4 and D 4. Baicalein also reduced release of LTC 4 and D 4 from the sensitized lung after antigen challenge. Baicalein relaxed the isolated guinea pig tracheal smooth muscle contracted by LTD 4, carbachol or histamine. However, this compound produced a contraction when the tracheal muscle was contracted by prostaglandin F 2α ( PGF 2α). This contraction by baicalein was abolished by pretreatment with indomethacin, a cyclooxygenase inhibitor. Baicalein elicited a relaxation in the normal non-sensitized tracheal preparation but a contraction in the tissue isolated from actively sensitized guinea pig in 4 among 7 cases. Baicalein also produced a contraction in the trachea pretreated with phorbol dibutyrate and contracted by carbachol, which was eliminated after treatment with indomethacin. The results suggest that baicalein exerts action via, at least, two different mechanisms, the inhibition of releasing SRS-A (LTs) and direct relaxing effects on the trachea. Besides, baicalein seems to produce contraction under certain conditions, which may involve stimulation of the cyclooxygenase pathway.

Development of control material for hemoglobin analysis

2012 ◽  
Vol 50 (2) ◽  
Author(s):  
Sakorn Pornprasert ◽  
Kunyakan Kongthai ◽  
Jarurin Waneesorn ◽  
Kanokwan Jaiping ◽  
Kallayanee Treesuwan
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Hplc Analysis ◽  
Freeze Drying ◽  
Sucrose Solution ◽  
Control Material ◽  
Normal Individuals ◽  
Thalassemia Trait

AbstractThere is no certified control material for hemoglobin analysis which has the hemoglobin (Hb)AWashed and dialysed erythrocytes of normal individuals and patients with β-thalassemia trait, HbE trait, β-thalassemia/HbE disease, homozygous HbE were lysed in 5% sucrose solution. The lyophilized hemoglobin control materials were prepared by using a freeze-drying (lyophilization) method. The high performance liquid chromatography (HPLC) analysis of lyophilized hemoglobin was performed after storing at –20°C for 1, 15 and 30 days and for 3 months.The chromatograms of lyophilized hemoglobin control materials showed similar patterns and similar levels of HbA, HbAThe lyophilized hemoglobin could be developed and used as control materials for hemoglobin analysis.

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