scholarly journals Gas chromatography-mass spectrometry chemical profile investigation and biological activities of ethylacetate fraction of Baobab (Adansonia digitata L.) pulp used in the treatment of urinary tract infections

2021 ◽  
Vol 5 (1) ◽  
Author(s):  
Kehinde O. Fagbemi ◽  
Daniel A. Aina ◽  
Roger M. Coopoosamy ◽  
Olufunmiso O. Olajuyigbe
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Urinary Tract ◽  
Urinary Tract Infections ◽  
Biological Activities ◽  
Gas Chromatography Mass Spectrometry ◽  
Chemical Profile ◽  
Adansonia Digitata ◽  
Chromatography Mass Spectrometry ◽  
Tract Infections

scholarly journals Gas chromatography-mass spectrometry chemical profile investigation and biological activities of ethylacetate fraction of Baobab (Adansonia digitata L.) pulp used in the treatment of urinary tract infections

2022 ◽  
Vol 6 (1) ◽  
Author(s):  
Kehinde O. Fagbemi ◽  
Daniel A. Aina ◽  
Roger M. Coopoosamy ◽  
Olufunmiso O. Olajuyigbe
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Urinary Tract ◽  
Bioactive Compounds ◽  
Biological Activities ◽  
Gas Chromatography Mass Spectrometry ◽  
Fruit Pulp ◽  
Adansonia Digitata ◽  
Chromatography Mass Spectrometry ◽  
Ms Analysis

Background: As a result of high percentage of women infected with urinary tract infection (UTI) annually, many rural dwellers use Adansonia digitata fruit pulp as herbal cure. Adansonia digitata L. (Malvaceae) is one of the great trees idolised in Africa because of its therapeutic properties.Aim: This study aimed at identifying chemical compounds in the ethylacetate fraction of the baobab fruit pulp and indicated their biological activities to justify its use for the folkloric treatment of UTI.Methods: The crude extract from Baobab fruit pulp was partitioned and ethylacetate extract was used for assay. Gas chromatography-mass spectrometry (GC–MS) analysis was performed to identify the bioactive compounds in the ethylacetate fraction, antioxidant activity was evaluated using 2, 2-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP) assays whilst the total phenolic and flavonoid contents were determined by already adopted protocols. The antibacterial properties were tested against some urinary tract pathogens using macro broth dilution method.Results: A total of 36 bioactive compounds were identified by GC–MS analysis, most of them have been reported as antibacterial, antioxidant, antidiabetic, antiasthma, antifungal, anti-inflammatory and anti-cancer agents. The extract exhibited highest activity against Pseudomonas aeruginosa American Type Culture Collection (ATCC) 19582 (1.22 mg/mL) whilst Escherichia coli ATCC 8739 (˃ 8.6 mg/mL) displayed high level of resistance amongst the five bacterial isolates used in this study.Conclusion: These findings indicated that the extract contained bioactive compounds of therapeutic importance with significant antioxidant and antibacterial potentials and justify the folkloric use of this fruit in the treatment of UTIs by many African dwellers.

Identification of Bioactive Phytocomponents of Hydroalcoholic Extract of Enhalus acoroides by Gas Chromatography- Mass Spectrometry Analysis

2021 ◽  
pp. 6511-6515
Author(s):  
S. Navaith Ahmed ◽  
P. Kalaivani ◽  
P. Amudha ◽  
B. Usharani
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Biological Activities ◽  
Marine Species ◽  
Mass Spectrometry Analysis ◽  
Gas Chromatography Mass Spectrometry ◽  
Hydroalcoholic Extract ◽  
Spectrometry Analysis ◽  
Enhalus Acoroides ◽  
Chromatography Mass Spectrometry

Sea grass are good source of potent drug which has medicinal properties and able to cure human disease. One such marine plant is the Enhalus acoroides which belong to hydrocharitaeceae family. This marine species are widely distributed in the tropics of Indian and western Pacific Ocean and the species were collected from Ramanathapuram district for further process. This study reveals about the bioactive components present in Enhalus acoroides and identified its biological activity by Gas chromatography Mass spectrometry analysis using hydroalcoholic extract. The compounds present in Enhalus acoroides are Benzoic acid, 2- methyl 7- oxa bicyclol heptanes, 1, 3 Nonadiene, silane, ethoxytriethyl. Biological activities of the compounds present in the sample include antioxidant, hypocholesterolemic, antihypertensive, anti-inflammatory, anti-microbial, antiviral and anti-hepatotoxic effect.

scholarly journals GAS CHROMATOGRAPHY–MASS SPECTROMETRY ANALYSIS OF METHANOL EXTRACT OF ZANTHOXYLUM RHETSA DC. FRUITS

2018 ◽  
Vol 11 (12) ◽  
pp. 247
Author(s):  
Kuladip Gurav ◽  
Varsha Jadhav (rathod)
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Bioactive Compounds ◽  
Biological Activities ◽  
Acid Methyl Ester ◽  
Mass Spectrometry Analysis ◽  
Gas Chromatography Mass Spectrometry ◽  
Hexadecanoic Acid ◽  
Spectrometry Analysis ◽  
Chromatography Mass Spectrometry

Objective: The aim was to investigate important bioactive compounds, biological activities, and medicinal importance of Zanthoxylum rhetsa fruits.Methods: The present work was carried out by gas chromatography–mass spectrometry (GC-MS) method for the identification of different compounds.Result: The methanolic extract of fruits showed 32 chemical compounds which are identified through GC-MS analysis. Among them, some of the compound names and percentage values are as follows: 2-propanone, 1,3-dihydroxy (48.9%), 4H-pyran-4-one,2,3-dihydro-3,5-dihydroxy-6-methyl (33.7%), 2-furancarboxaldehyde, 5-[hydroxymethyl] (50.2%), 1-Heptatriacontanol (34.4%), 9,12-octadecadienoic acid (zz)- (48.6%), cholestan- 3-ol,2-methylene, [3β,5α] (75.0%), 4H-pyran-4-one, 2,3-dihydro-3,5-dihydroxy-6-methyl (90.8), 2-furancarboxaldehyde, 5-[hydroxymethyl] (92.0%), hydroquinone (64.9%), n-hexadecanoic acid (37.5%), octadecanoic acid (28.2%), 9,12,15, octadecatrienoic acid, 2-[(trimethylsiyl]oxy]-1- [(trimethylsiyl]oxy]methyl] ethyl ester, [zzz] (22.6%), 9-hexadecanoic acid (10.3%), digitoxin (18.8%), 8,11,14-eicosatrienoic acid methyl ester, [zzz] (25.5%), and oleic acid (16.5%). Most of the identified compounds in the crude methanolic extracts exhibit some bioactivities, namely anticancer, anti-inflammatory, antimicrobial, hepatoprotective, antioxidant, hypocholesterolemic, nematicide, pesticide, anti-androgenic flavor, hemolytic, 5-alpha-reductase inhibitor, insectifuge, antiarthritic, anti-coronary, cardiovascular, anti-breast cancer, aromatic, and insectifuge. On the basis of the above investigation, the fruits can be recommended as a treasure of bioactive compounds and it plays a promising role in herbal medicine.Conclusion: The present study reveals that fruits of Z. rhetsa contain various bioactive compounds. Digitoxin is recorded in the ripened fruit of Z. rhetsa and it shows the anticancerous and cardiac arrest properties. Hence, in future, this plant will play a promising role in curing cancer.

scholarly journals Initial study of three different pathogenic microorganisms by gas chromatography-mass spectrometry

F1000Research ◽  
2018 ◽  
Vol 6 ◽  
pp. 1415 ◽  
Author(s):  
Najmeh Karami ◽  
Fateme Mirzajani ◽  
Hassan Rezadoost ◽  
Abdollah Karimi ◽  
Fatemeh Fallah ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Respiratory Tract ◽  
Volatile Compounds ◽  
Solid Phase ◽  
Late Phase ◽  
Accurate Method ◽  
Gas Chromatography Mass Spectrometry ◽  
Chromatography Mass Spectrometry ◽  
Tract Infections

Background: Diagnoses  of  respiratory  tract  infections  usually happen  in  the  late  phase  of  the  disease  and  usually  result  in  reduction  of  the  pathogen  load after broad-spectrum  antibiotic  therapy,  but  not  in eradication of the pathogen.  The  development  of a  non-invasive,  fast,  and  accurate  method  to  detect  pathogens  has  always  been  of  interest  to  researchers  and  clinicians  alike.  Previous studies have shown that bacteria produce organic gases.  The  current  study  aimed  to  identify  the  volatile  organic  compounds  (VOCs)  produced  by three  respiratory  tract  pathogens,  including  Staphylococcus  aureus,  Escherichia  coli  and  Candida  albicans. Methods: The  VOCs  produced  were identified by gas chromatography–mass spectrometry (GC-MS), with  prior  collection  of  microbial  volatile  compounds  using  solid  phase  microextraction  (SPME)  fiber.  The volatile compounds were collected by obtaining bacterial headspace samples. Results: Results  showed  that  these  three  organisms  have  various  VOCs,  which  were  analyzed  under  different  conditions.  By ignoring common VOCs, some species-specific VOCs could be detected.  The most important VOC of E. coli was indole, also some important VOCs produced by S. aureus  were 2,3-pentandione,  cis-dihydro-α-terpinyl  acetate,  1-decyne,  1,3-heptadiene,  2,5-dimethyl  pyrazine,  ethyl  butanoate  and  cyclohexene,4-ethenyl. Furthermore,  most  of the identified  compounds  by  C.  albicans are  alcohols. Conclusions: The  detection  of  VOCs  produced  by  infectious  agents  maybe  the  key  to  make   a  rapid  and  precise  diagnosis  of  infection,  but  more  comprehensive  studies  must  be  conducted  in this  regard.

scholarly journals ANALYSIS OF BIOLOGICAL ACTIVITY AND GAS CHROMATOGRAPHY-MASS SPECTROMETRY STUDY OF CONVENTIONAL EXTRACTION OF VITEX NEGUNDO LINN. LEAVES

2019 ◽  
Vol 12 (1) ◽  
pp. 289 ◽  
Author(s):  
Samreen Fatema ◽  
Milind Ubale ◽  
Mazahar Farooqui ◽  
Pathan Mohd Arif
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Biological Activity ◽  
Leaf Extract ◽  
Antioxidant Activities ◽  
Biological Activities ◽  
Gas Chromatography Mass Spectrometry ◽  
Negative Results ◽  
Chromatography Mass Spectrometry ◽  
Mass Spectrometry Study

Objective: The main objective of the present work is to carry out the biological activity, gas chromatography-mass spectrometry (GC-MS) studies for the possible compounds present in Vitex negundo.Methods: The aqueous extract of V. negundo Linn. was screened for biological activities such as antimicrobial, antituberculosis (TB), antimalarial, and antioxidant activities. The GC-MS analysis was carried out.Results: The result shows that leaf extract is effective against Escherichia Coli and Bacillus subtitus while negative results for anti-TB and anti-malarial activity. The antioxidant activity of the leave extract is excellent.Conclusion: The compounds present in the leaf extract of V. negundo are responsible for possessing the biological activity.

scholarly journals Gas chromatography-mass spectrometry analysis of phytocomponents of sargassum polycystum

2019 ◽  
Vol 10 (4) ◽  
pp. 2705-2710
Author(s):  
Nurul Najwa Zamimi ◽  
Noorhazayti Ab. Halim ◽  
Deny Susanti Darnis ◽  
Widya Lestari ◽  
Md Muziman Syah Md Mustafa
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Biological Activities ◽  
Brown Seaweed ◽  
Mass Spectrometry Analysis ◽  
Gas Chromatography Mass Spectrometry ◽  
Sargassum Polycystum ◽  
Spectrometry Analysis ◽  
Chromatography Mass Spectrometry ◽  
Ms Analysis

Sargassum polycystum is a brown seaweed, which has been reported to contain various phytoconstituents especially antimicrobial properties. However, the study on phytochemical components and biological activities of S. polycystum are yet fully understood. Therefore, the objectives of this study are to evaluate the best extraction solvents for S. polycystum and to determine the percentage of phytocomponents obtained in the n-hexane, dichloromethane (DCM) and methanol extracts of S. polycystum via Gas Chromatography-Mass Spectrometry (GC-MS) analysis. S. polycystum was collected from the coastal area of Sabah, Malaysia. After collection, it was rinsed, dried and extracted with n-hexane, DCM and methanol by soxhlet extraction. The fatty acid compounds (FAME) analysis was done using The Perkin Elmer Turbo Mass Spectrometer.  This study showed that methanol is the most efficient solvent as it produced the highest extraction yield with 3.83%, followed by n-hexane with 1.69% and lastly DCM with 0.59% in S. polycystum. On top of that, this study has found 19 phytocomponents in all extraction methods of S. polycystum, which have been proven to possess antibacterial constituents such as palmitic acid, myristic acid, oleic acid, pentadecanoic acid and behenic acid as assessed by GC-MS analysis.

scholarly journals Initial study of three different pathogenic microorganisms by gas chromatography-mass spectrometry

F1000Research ◽  
2017 ◽  
Vol 6 ◽  
pp. 1415
Author(s):  
Najmeh Karami ◽  
Fateme Mirzajani ◽  
Hassan Rezadoost ◽  
Abdollah Karimi ◽  
Fatemeh Fallah ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Respiratory Tract ◽  
Volatile Compounds ◽  
Solid Phase ◽  
Late Phase ◽  
Accurate Method ◽  
Gas Chromatography Mass Spectrometry ◽  
Chromatography Mass Spectrometry ◽  
Tract Infections

Background: Diagnoses  of  respiratory  tract  infections  usually happen  in  the  late  phase  of  the  disease  and  usually  result  in  reduction  of  the  pathogen  load after broad-spectrum  antibiotic  therapy,  but  not  in eradication of the pathogen.  The  development  of a  non-invasive,  fast,  and  accurate  method  to  detect  pathogens  has  always  been  of  interest  to  researchers  and  clinicians  alike.  Previous studies have shown that bacteria produce organic gases.  The  current  study  aimed  to  identify  the  volatile  organic  compounds  (VOCs)  produced  by three  respiratory  tract  pathogens,  including  Staphylococcus  aureus,  Escherichia  coli  and  Candida  albicans. Methods: The  VOCs  produced  were identified by gas chromatography–mass spectrometry (GC-MS), with  prior  collection  of  microbial  volatile  compounds  using  solid  phase  microextraction  (SPME)  fiber.  The volatile compounds were collected by obtaining bacterial headspace samples. Results: Results  showed  that  these  three  organisms  have  various  VOCs,  which  were  analyzed  under  different  conditions.  By ignoring common VOCs, some species-specific VOCs could be detected.  The most important VOC of E. coli was Indole, also some important VOCs produced by S. aureus  were 2,3-Pentandione,  cis-Dihydro-α-terpinyl  acetate,  1-Decyne,  1,3-Heptadiene-3-yne,  2,5-dimethyl  Pyrazine,  Ethyl  butanoate  and  Cyclohexene,4-ethenyl  furthermore,  most  of  identified  compounds  by  C.  albicans are  alcohols. Conclusions: The  detection  of  VOCs  produced  by  infectious  agents  maybe  the  key  to  make   a  rapid  and  precise  diagnosis  of  infection,  but  more  comprehensive  studies  must  be  conducted  in this  regard.

scholarly journals Gas chromatography-Mass spectrometry analysis of bioactive compounds in chloroform extract of Psoralea corylifolia L.

2021 ◽  
Vol 13 (4) ◽  
pp. 1225-1230
Author(s):  
S. Ranjith Kumar ◽  
K. Chozhan ◽  
K. A. Murugesh ◽  
R. Rajeswari ◽  
K. Kumaran
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Bioactive Compounds ◽  
Biological Activities ◽  
Chloroform Extract ◽  
Mass Spectrometry Analysis ◽  
Gas Chromatography Mass Spectrometry ◽  
Psoralea Corylifolia ◽  
Active Compounds ◽  
Chromatography Mass Spectrometry

Psoralea corylifolia is the most important medicinal plant which has various secondary metabolites and its leaves are broadly used in many sectors due to its antimicrobial activity. This study aimed to find the bio-active compounds of chloroform extract of P. corylifolia leaves using Perkin-Elmer Gas Chromatography – Mass Spectrometry (GC-MS). The results of GC-MS compounds in the chloroform extract was appropriate to the database of National Institute of Standards and Technology (NIST). GC-MS analysis of chloroform extract of P. corylifolia leaves reveal the presence of bioactive compounds as Hexadecanoic acid, 3-hydroxy-, methyl ester , Hydroxylamine, O-decyl- , 2,4-Di-tert-butylphenol ) Cubenol , Neophytadiene , Phytol , Linoleic acid ethyl esterand 9,12,15-Octadecatrienoic acid, ethyl ester, (Z,Z,Z)- ) . These bio-active compounds are mainly responsible for various biological activities. Hence, this study will make a good way for the production of various products for curing various disease-causing pathogens by using P. corylifolia leaves.

scholarly journals Diagnosis of three different pathogenic microorganisms by gas chromatography-mass spectrometry

F1000Research ◽  
2017 ◽  
Vol 6 ◽  
pp. 1415 ◽  
Author(s):  
Najmeh Karami ◽  
Fateme Mirzajani ◽  
Hassan Rezadoost ◽  
Abdollah Karimi ◽  
Fatemeh Fallah ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Volatile Organic Compounds ◽  
Respiratory Tract ◽  
Volatile Compounds ◽  
Organic Compounds ◽  
Gas Chromatography Mass Spectrometry ◽  
Chromatography Mass Spectrometry ◽  
Volatile Organic ◽  
Tract Infections

Background: Diagnoses  of  respiratory  tract  infections  usually happen  in  the  late  phase  of  the  disease  and  usually  result  in  reduction  of  the  pathogen  load after broad-spectrum  antibiotic  therapy,  but  not  in eradication of the pathogen.  The  development  of a  non-invasive,  fast,  and  accurate  method  to  detect  pathogens  has  always  been  of  interest  to  researchers  and  clinicians  alike.  Previous studies have shown that bacteria produce organic gases.  The  current  study  aimed  to  identify  the  volatile  organic  compounds  (VOCs)  produced  by three  respiratory  tract  pathogens,  including  Staphylococcus  aureus,  Escherichia  coli  and  Candida  albicans. Methods: The  volatile  organic  compounds  (VOCs)  produced  were identified by gas chromatography–mass spectrometry(GC-MS), with  prior  collection  of  microbial  volatile  compounds  using  solid  phase  microextraction  (SPME)  fiber.  The volatile compounds were collected by obtaining bacterial headspace samples. Results: Results  showed  that  these  three  organisms  have  various  VOCs,  which  were  analyzed  under  different  conditions.  By ignoring common VOCs, some species-specific VOCs could be detected.  The most important VOC of E. coli was Indole, also some important VOCs produced by S. aureus  were 2,3-Pentandione,  cis-Dihydro-α-terpinyl  acetate,  1-Decyne,  1,3-Heptadiene-3-yne,  2,5-dimethyl  Pyrazine,  Ethyl  butanoate  and  Cyclohexene,4-ethenyl  furthermore,  most  of  identified  compounds  by  C.  albicans are  alcohols. Conclusions: The  detection  of  VOCs  produced  by  infectious  agents  maybe  the  key  to  make   a  rapid  and  precise  diagnosis  of  infection,  but  more  comprehensive  studies  must  be  conducted  in this  regard.

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