scholarly journals Spectrophotometric and computerized evaluation of tooth bleaching employing 10 different home-bleaching procedures: In-vitro study

2014 ◽  
Vol 08 (04) ◽  
pp. 538-545 ◽  
Author(s):  
Cem Peskersoy ◽  
Ayhan Tetik ◽  
Veli Ozgen Ozturk ◽  
Necmi Gokay
Keyword(s):  
Digital Imaging ◽  
In Vitro Study ◽  
Vitro Study ◽  
Tooth Bleaching ◽  
Control Group ◽  
San Jose ◽  
Color Changes ◽  
Before And After ◽  
Effective Product

ABSTRACT Objective: The aim of this in-vitro study was to evaluate the efficacy of bleaching products, determine the applicability and validation of the measurement methods. Materials and Methods: Freshly extracted 110 human incisor teeth were stained with whole blood and hemolysate solution prior to the application of 10 different home-bleaching products. Spectrophotometric measurements of the tooth shades were performed for each specimen before and after bleaching at the 1st, 3rd, 7th, and 14 days. Differences in lightness (Δl), chroma (Δc), hue (Δh) values and shade changes were measured to evaluate process. Computerized digital imaging analyses to determine the color changes were performed with Photoshop CS4 software (Adobe, San Jose, CA, USA). Statistical analyses were performed with analysis of variance, Scheffe and Tukey tests. Results: In all of the test groups regardless of the material used, a significant increase in lightness and hue, and decrease of chroma were observed, as compared to the control group. After recommended bleaching applications, Δl and Δh values respectively increased in group Zaris White and Brite (ZWB) and group Pola Night and Δc values showed significant decrease in groups ZWB and Rembrandt REM3 (P < 0.05). At the end of the procedure both spectrophotometric and digital imaging analysis showed ZWB was the most effective product among the others while Yotuel and Happy Smile were the least (P < 0.05). Conclusions: Home-bleaching systems showed slower but almost permanent bleaching effect likewise office-based methods. Both software and spectrophotometric analyses have advantages such as evaluating the results objectively and numerically, also treatment outcomes could be preserved.

scholarly journals Effect of Citric Acid on Color Changes of Calcium Silicate-Based Cements an In Vitro Study

2021 ◽  
Vol 11 (5) ◽  
pp. 2339
Author(s):  
Joanna Metlerska ◽  
Till Dammaschke ◽  
Mariusz Lipski ◽  
Irini Fagogeni ◽  
Anna Machoy-Mokrzyńska ◽  
...  
Keyword(s):  
Citric Acid ◽  
Calcium Silicate ◽  
In Vitro Study ◽  
Vitro Study ◽  
Color Measurement ◽  
Color Changes ◽  
Naked Eye ◽  
Root Canal Irrigants ◽  
The Difference

The aim of the present in vitro study was to investigate the effects of 10% and 40% citric acid (CA) on the color of calcium silicate–based cements (CSCs) in comparison to the effects of common root canal irrigants. Samples of six CSCs (n = 6)—ProRoot MTA (Dentsply, Tulsa, OK, USA), Biodentine (Septodont, Saint-Maur-des-Fossés, France), MTA Plus (Avalon Biomed Inc, by Prevest Denpro Limited, Jammu, India), MTA Repair HP (Angelus, Londrina, PR, Brazil), Ortho MTA (BioMTA, Seoul, Korea), and Retro MTA (BioMTA, Seoul, Korea)—were immersed in 10% and 40% CA as well as 15% EDTA, 2% NaOCl, 2% CHX, and 0.9% NaCl for 15 min, 1 h, and 24 h. ΔE values, representing the difference between the final and baseline values of the color components, were then determined using a VITA Easyshade Compact 5.0 spectrophotometer. Naked-eye evaluation of the changes in color and structures of the materials was performed using our own scale. Upon immersion of the materials in both 10% and 40% CA, there were statistically significant differences between spectrophotometric color measurement results for all CSCs (P < 0.05). However, CA does not cause dark discoloration, observable with the naked eye, of any of the materials, such as NaOCl and CHX. Significant statistical differences were also found between all CSCs in terms of submersion duration (P < 0.05). CA, which could be an alternative to EDTA use, caused greater CSCs discoloration and changed some of their structures. Unless required by the therapeutic procedure, clinicians should pay attention to the fact that the irrigant may affect the CSCs discoloration and minimize the contact time of irrigant with CSCs.

scholarly journals Effect of topical fluoride on microshear bond strength of primary enamel to composite, microhardness of enamel and its surface morphology: An in vitro study

2019 ◽  
Vol 13 (4) ◽  
pp. 305-310
Author(s):  
Mina Biria ◽  
Sajedeh Namaei Ghasemi ◽  
Seyedeh Mahsa Sheikh-Al-Eslamian ◽  
Narges Panahandeh
Keyword(s):  
Bond Strength ◽  
Artificial Saliva ◽  
Morphological Characteristics ◽  
In Vitro Study ◽  
Vitro Study ◽  
Control Group ◽  
Modes Of Failure ◽  
Group 3 ◽  
Microshear Bond Strength

Background. This in vitro study aimed to evaluate the microshear bond strength (μSBS), microhardness and morphological characteristics of primary enamel after treating with sodium fluoride (NaF) and acidulated phosphate fluoride (APF). Methods. Forty-eight primary canines were cut into mesial and distal sections and assigned to five groups randomly: group 1 (immersed in saliva as a control), group 2 (treated with NAF and immersed in saliva for 30 minutes), group 3 (treated with APF and immersed in saliva for 30 minutes), group 4 (treated with NAF and immersed in saliva for 10 days), and group 5 (treated with APF and immersed in saliva for 10 days). Composite resin (Filtek Z250) was bonded on the specimens (n=15) for measuring the μSBS. After storage in 37°C artificial saliva for 24 hours, µSBS and Vickers hardness tests (10 readings) were performed. The data were analyzed using one-way ANOVA and Kolmogorov-Smirnov, Levene’s and Tukey HSD tests (P<0.05). Morphological analysis of enamel and modes of failure were carried out under a scanning electron microscope (SEM) on two remaining specimens. Results. Significant differences in μSBS were only noted between groups 2 and 4 (P=0.024). Group 3 showed a significant decrease in hardness after storage in artificial saliva (P<0.001), with a significantly lower hardness than the other groups (P<0.001). The SEM observations showed irregular particles in groups 3 and 5; uniform, smooth and thin coats were seen in groups 2 and 4. Conclusion. Fluoride therapy with NaF and APF gels prior to restorative treatments had no adverse effects on the microshear bond strength.

scholarly journals In Vitro Study on the Effect of a New Bioactive Desensitizer on Dentin Tubule Sealing and Bonding

2020 ◽  
Vol 11 (2) ◽  
pp. 38
Author(s):  
Minh N. Luong ◽  
Laurie Huang ◽  
Daniel C. N. Chan ◽  
Alireza Sadr
Keyword(s):  
Bond Strength ◽  
In Vitro Study ◽  
Vitro Study ◽  
Acid Pretreatment ◽  
Universal Adhesive ◽  
Cross Sectional ◽  
Before And After ◽  
Human Dentin ◽  
Microshear Bond Strength

Bioactive mineral-based dentin desensitizers that can quickly and effectively seal dentinal tubules and promote dentin mineralization are desired. This in vitro study evaluated a novel nanohydroxyapatite-based desensitizer, Predicta (PBD, Parkell), and its effect on bond strength of dental adhesives. Human dentin discs (2-mm thick) were subjected to 0.5 M EDTA to remove the smear layer and expose tubules, treated with PBD, and processed for surface and cross-sectional SEM examination before and after immersion in simulated body fluid (SBF) for four weeks (ISO 23317-2014). The effects of two dental desensitizers on the microshear bond strength of a universal adhesive and a two-step self-etch system were compared. SEM showed coverage and penetration of nanoparticles in wide tubules on the PBD-treated dentin at the baseline. After four weeks in SBF, untreated dentin showed amorphous mineral deposits while PBD-treated dentin disclosed a highly mineralized structure integrated with dentin. Desensitizers significantly reduced microshear bond strength test (MSBS) of adhesives by 15–20% on average, depending on the bonding protocol. In conclusion, PBD demonstrated effective immediate tubules sealing capability and promoted mineral crystal growth over dentin and into the tubules during SBF-storage. For bonding to desensitizer-treated dentin, a two-step self-etching adhesive or universal bond with phosphoric acid pretreatment are recommended.

scholarly journals Effects of CO2laser irradiation on matrix-rich biofilm development formation–an in vitro study

PeerJ ◽  
2016 ◽  
Vol 4 ◽  
pp. e2458 ◽  
Author(s):  
Bruna Raquel Zancopé ◽  
Vanessa B. Dainezi ◽  
Marinês Nobre-dos-Santos ◽  
Sillas Duarte ◽  
Vanessa Pardi ◽  
...  
Keyword(s):  
Contact Angle ◽  
Biofilm Formation ◽  
Dental Enamel ◽  
In Vitro Study ◽  
Dry Weight ◽  
Vitro Study ◽  
Enamel Surface ◽  
Control Group ◽  
Biofilm Development

BackgroundA carbon dioxide (CO2) laser has been used to morphologically and chemically modify the dental enamel surface as well as to make it more resistant to demineralization. Despite a variety of experiments demonstrating the inhibitory effect of a CO2laser in reduce enamel demineralization, little is known about the effect of surface irradiated on bacterial growth. Thus, this in vitro study was preformed to evaluate the biofilm formation on enamel previously irradiated with a CO2laser (λ = 10.6 µM).MethodsFor this in vitro study, 96 specimens of bovine enamel were employed, which were divided into two groups (n = 48): 1) Control-non-irradiated surface and 2) Irradiated enamel surface. Biofilms were grown on the enamel specimens by one, three and five days under intermittent cariogenic condition in the irradiated and non-irradiated surface. In each assessment time, the biofilm were evaluated by dry weigh, counting the number of viable colonies and, in fifth day, were evaluated by polysaccharides analysis, quantitative real time Polymerase Chain Reaction (PCR) as well as by contact angle. In addition, the morphology of biofilms was characterized by fluorescence microscopy and field emission scanning electron microscopy (FESEM). Initially, the assumptions of equal variances and normal distribution of errors were conferred and the results are analyzed statistically by t-test and Mann Whitney test.ResultsThe mean of log CFU/mL obtained for the one-day biofilm evaluation showed that there is statistical difference between the experimental groups. When biofilms were exposed to the CO2laser, CFU/mL and CFU/dry weight in three day was reduced significantly compared with control group. The difference in the genes expression (Glucosyltransferases (gtfB) and Glucan-binding protein (gbpB)) and polysaccharides was not statically significant. Contact angle was increased relative to control when the surface was irradiated with the CO2laser. Similar morphology was also visible with both treatments; however, the irradiated group revealed evidence of melting and fusion in the specimens.ConclusionIn conclusion, CO2laser irradiation modifies the energy surface and disrupts the initial biofilm formation.

scholarly journals Effect of external tooth bleaching on the surface of resin composites – An in vitro study

2015 ◽  
Vol 56 (3) ◽  
pp. 149-155
Author(s):  
Helena Maria Gorgulho Pitacas ◽  
Alexandre Cavalheiro ◽  
Catarina Coito ◽  
Ana Silva ◽  
Raquel Eira ◽  
...  
Keyword(s):  
In Vitro Study ◽  
Vitro Study ◽  
Tooth Bleaching ◽  
Resin Composites

scholarly journals Erratum to: Efficiency of powered systems for interproximal enamel reduction (IER) and enamel roughness before and after polishing—an in vitro study

2016 ◽  
Vol 20 (8) ◽  
pp. 2323-2323
Author(s):  
Sebastian Zingler ◽  
Andreas Sommer ◽  
Sinan Sen ◽  
Daniel Saure ◽  
Jochen Langer ◽  
...  
Keyword(s):  
In Vitro Study ◽  
Vitro Study ◽  
Enamel Roughness ◽  
Before And After ◽  
Enamel Reduction

The permeability of natural dentine caries before and after restoration: An in vitro study

2007 ◽  
Vol 35 (8) ◽  
pp. 656-663 ◽  
Author(s):  
Taher O. Elgalaid ◽  
Steve L. Creanor ◽  
Siobhan Creanor ◽  
Andrew F. Hall
Keyword(s):  
In Vitro Study ◽  
Vitro Study ◽  
Before And After

scholarly journals Comparative evaluation of force decay pattern in orthodontic active tiebacks exposed to five different mouth rinses: An in vitro Study

2020 ◽  
Vol 14 (4) ◽  
pp. 244-249
Author(s):  
Amir Hossein Mirhashemi ◽  
Atefe Saffar Shahroudi ◽  
Keyvan Shahpoorzadeh ◽  
Niloofar Habibi Khameneh
Keyword(s):  
In Vitro Study ◽  
Vitro Study ◽  
Control Group ◽  
Mouth Rinses ◽  
Force Decay ◽  
Force Relaxation ◽  
Decay Pattern ◽  
Initial Force ◽  
And Control

Background. This study compared the force decay pattern of two different orthodontic active tiebacks (ATBs) exposed to five different commercially available mouth rinses. Methods. In this in vitro study, 90 transparent ATBs and 90 gray ATBs were divided into six groups; one was the control group, and the others were exposed to one of these mouth rinses twice a day for 60 seconds: Listerine, chlorhexidine, Orthokin, Persica, and fluoride. The initial force of each ATB was 250 g at a 24-mm extension. The force of ATBs was measured on days 1, 7, 14, and 28 using a digital gauge. Results. The highest percentage of force loss was observed between days 14 and 28 (P<0.05). At the end of the study, the Persica group exhibited the highest force degradation in both ATB types. In the transparent ATBs, it was followed by Orthokin, Listerine, fluoride, chlorhexidine, and control groups, respectively. In the gray ATBs, Orthokin, chlorhexidine, control, Listerine, and fluoride groups exhibited the highest force decay in descending order. In some groups, the differences between transparent and gray ATBs were significant. In the control group, the force of transparent ATB was significantly higher than gray ones on days 7 and 14 but not significantly after four weeks. Conclusion. ATBs’ force degradation could be exacerbated by the use of some mouth rinses. There were some differences between force relaxation patterns of transparent and gray ATBs. The data could be beneficial in choosing appropriate O-rings for making ATBs.

scholarly journals Analysis of Surface Roughness and 3D Scanning Topography of Zirconia Implants Before and After Photofunctionalisation by 3D Optical Profilometry- An Invitro Study

2021 ◽  
Author(s):  
Arunjaikumar Ravindran ◽  
Suma Karthigeyan ◽  
Ramesh Bhat ◽  
Madhulika Naidu ◽  
Senthilnathan Natarajan ◽  
...  
Keyword(s):  
Surface Roughness ◽  
Roughness Parameter ◽  
In Vitro Study ◽  
Control Group ◽  
P Value ◽  
Study Group ◽  
Optical Profilometry ◽  
Before And After ◽  
Zirconia Implants

Introduction: Zirconia is considered as an alternative material for the fabrication of implants. Surface roughness of the implant plays a fundamental role in the initial bone formation. The rationale of using 3D optical profilometry in this study was to evaluate the surface roughness of the zirconia implants before and after Ultraviolet (UV) photofunctionalisation. Also, Moreso 3D optical instruments have a better resolution than the mechanical ones like Atomic Force Microscopy (AFM). Aim: To analyse the surface roughness and topography of zirconia implants after photofunctionalisation using optical profilometry. Materials and Methods: This is an in-vitro study conducted over a period of six months from March 2020 to August 2020. Ten commercially machined Zirconia implants, five each in study and control group, were micro analysed at three different regions (abutment, thread and crest) by optical profilometry. Study group was surface treated by UV radiation for 48 hours. Quantitative morphometric analysis was done between two groups and p-value less than 0.05 was considered statistically significant. The statistical test applied in this study was independent t-test. Results: Scanning micrographs of the study group revealed highest density of summits contributing to increased surface area in the study group. Quantitative analysis of surface roughness showed statistically significant higher mean roughness parameter for photofunctionalised implants in abutment, crest and thread region (p<0.05). Conclusion: Photofunctionalisation is a potentially synergistic technique in producing textured zirconia implants.

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