scholarly journals Antidiabetic and antioxidant activity of ethyl acetate extract fraction of Moringa oleifera leaves in streptozotocin-induced diabetes rats via inhibition of inflammatory mediators

2018 ◽  
Vol 8 (6) ◽  
pp. 320 ◽  
Author(s):  
GhaziA Bamagous ◽  
SaeedS Al Ghamdi ◽  
IbrahimAbdel Aziz Ibrahim ◽  
AmalM Mahfoz ◽  
MohamedA Afify ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Inflammatory Mediators ◽  
Moringa Oleifera ◽  
Ethyl Acetate Extract ◽  
Moringa Oleifera Leaves ◽  
Streptozotocin Induced Diabetes ◽  
Extract Fraction

scholarly journals Aktivitas Antioksidan Ekstrak Etil Asetat Daun Kelor (Moringa Oleifera L)

2020 ◽  
Vol 3 (1) ◽  
pp. 13-21
Author(s):  
Rosalina Yuliana Kurang ◽  
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Extraction Method ◽  
Moringa Oleifera ◽  
Ethyl Acetate Extract ◽  
Phenolic Antioxidant ◽  
The Public ◽  
Activity Test ◽  
Dpph Method

Moringa oliefera L is one of the most plants in Indonesia. One area has this plant is Alor island with the type of Moringa leaves are flavorful and not flavorful. The use of Moringa leaves is not widely known by the public. The aim of this research is to determine the antioxidant activity of moringa leaves flavorful. The method used is the extraction method and the DPPH method. The results of the analysis showed that ethyl acetate extract of Moringa oliefera L, contained compounds group of flavonoids, terpenoids and phenolic. Antioxidant activity test showed that the ethyl acetate extract of Moringa oliefera L have a strong antioxidant activity with an IC50 14,301 ppm.

Identification of phytoconstituents of Tragia involucrata leaf extracts and evaluate their correlation with anti-inflammatory & antioxidant properties

2021 ◽  
Vol 20 ◽  
Author(s):  
Vinodhini Velu ◽  
Swagata Banerjee ◽  
Vidya Rajendran ◽  
Gaurav Gupta ◽  
Dinesh Kumar Chellappan ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Petroleum Ether ◽  
Ethyl Acetate Extract ◽  
Petroleum Ether Extract ◽  
Inflammatory Activity ◽  
Ether Extract ◽  
Membrane Stabilization ◽  
Leaf Extracts ◽  
Anti Inflammatory

Aims: The present investigation was aimed at exploring the phytoconstituents using Gas Chromatography Mass Spectroscopy and to evaluate antioxidant and anti-inflammatory properties of the leaf extracts. Materials and Methods: The extracts were obtained sequentially with petroleum ether, ethyl acetate and water using soxhlet apparatus. The anti-inflammatory property of the identified compounds using GC- MS spectroscopy was evaluated in silico. The antioxidant activity was performed by DPPH and H2O2 method whereas anti-inflammatory study was carried out by HRBC membrane stabilization method. Terpenoids were found to be major constituents in petroleum ether extract while, phenols and flavonoids were predominantly found in ethyl acetate extract. Results and Discussion: The GC-MS analysis of the extract revealed six major molecules including Squalene, 19β, 28- epoxyleanan-3-ol and 2-tu-Butyl-5-chloromethyl-3-methyl-4-oxoimidazolidine-1-carboxylic acid. The ethyl acetate extract showed a significant antioxidant activity (P<0.01) in both DPPH method (70.87 %) and H2O2 method (73.58%) at 200 µg mL-1 . Increased membrane stabilization of petroleum ether extract was observed in the in vitro anti-inflammatory activity study. A strong relationship between the terpenoid content and anti-inflammatory activity was obtained from the correlation (0.971) and docking study. Conclusion: These results justify T. involucrata to be a rich source of terpenoids with potent antiinflammatory property.

scholarly journals Formulation and Evaluation of Antioxidant Peel-Off Face Mask Containing Red Dragon Fruit Rind Extract (Hylocereus polyrhizus Haw.)

2020 ◽  
Vol 20 (3) ◽  
pp. 438
Author(s):  
Trasna Arman Jani ◽  
Aliefman Hakim ◽  
Yohanes Juliantoni
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Face Mask ◽  
Hexane Extract ◽  
Product Evaluations ◽  
Drying Time ◽  
Dragon Fruit ◽  
Dispersive Power

Peel-Off face mask is one of the cosmetics that is used to treat skin from free radicals. Peel-off face mask can minimize the effects of free radicals because it contains antioxidant. One of the antioxidant sources is red dragon fruit’s rind (Hylocereuspolyrhizus Haw.). The aim of this study is to obtain Peel-Off face mask of red dragon fruit’s rind extract that contains anthocyanin. Red dragon fruit’s rind was macerated by ethanol 96%, ethyl acetate and n-hexane added citric acid (4:1) solvents. TLC test used Chloroform: ethyl acetate: n-butanol (5:4:1) eluent and sprayed by AlCl3. Antioxidant activity of extract was tested using DPPH (1,1-Diphenyl-2-Picryhidrazyl) method. Peel-Off face mask was made using PVA, HPMC, methyl paraben, propylparaben, propylenglycol, extract, ethanol 96% and aquadest. The rendemen of ethanol 96% extract was 9,476%, ethyl acetate extract was 0,783% and n-hexane extract was 0,631%. The results of TLC test showed yellow spots on the ethyl acetate and ethanol 96% extracts which indicated that extract contained flavonoids (anthocyanin). The results of antioxidant activity test showed that IC50 value of ethanol 96% extract was 189,7422 (AAI=0,2087), ethyl acetate extract was 196,9398 (AAI=0,2011), and n-hexane extract was 385,3664 (AAI=0,1027). The result of the product evaluations showed that all the formulas complied the product requirements such as the organoleptic, homogenity, pH, dispersive power and drying time. Peel-off face mask already meet the product requirements but further research is needed to test the product stability and activity.

scholarly journals Comparative phytotoxicity and chironomid toxicity assessments of leaf successive extract fractions of Asiatic poison bulb, Crinum asiaticum L

2020 ◽  
Author(s):  
Souren Goswami ◽  
Sanjib Ray
Keyword(s):  
Ethyl Acetate ◽  
Petroleum Ether ◽  
Ethyl Acetate Extract ◽  
Antioxidant Properties ◽  
Ethyl Acetate Fraction ◽  
Meristematic Tissue ◽  
Aqueous Fraction ◽  
Extract Fraction ◽  
Potent Growth ◽  
Crinum Asiaticum

AbstractCrinum asiaticum is an evergreen bulbous perennial shrub of Amaryllidaceae family with ethnomedicinal importance and our earlier study described a comparative account antimicrobial and antioxidant properties of the different solvent-mediated sequential extract fractions. The present study aimed to analyze their comparative account of induced phytotoxicity and chironomid toxicity. For phytotoxicity assessment, germination inhibition and seedling’s root and shoot growth retardation effects on Triticum aestivum and Cicer arietinum were analyzed and for lethal concentration determination, the freshwater bottom-dwelling first instars chironomid larvae were used. The crude aqueous, petroleum ether and ethyl acetate extracts showed significant toxic effects on both meristematic tissue and aquatic midges. The phytotoxic assays indicate that the ethyl acetate fraction contains the most potent growth inhibitors, followed by the crude aqueous and petroleum ether fractions. The last aqueous fraction was found to be the least toxic, the highest LC50 and LT50 values and ethyl acetate extract fraction having highest toxicity. Thus the present study supplements to our earlier report, that indicated the last aqueous extract fraction of C. asiaticum has potent antioxidant and antibacterial potentials as well as its prospective use in livestock maintenance, as it is least toxic and the ethyl acetate extract, the most toxic fraction identified here, is needed to explore for pharmaceutical importance.

scholarly journals The ethyl acetate fraction of Moringa oleifera leaves effects on endothelial stress in rat sepsis model

2019 ◽  
Vol 8 (3) ◽  
pp. 940
Author(s):  
Tatar Sumandjar ◽  
Bambang Purwanto ◽  
Brian Wasita ◽  
Dono Indarto ◽  
Risya Cilmiaty ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Moringa Oleifera ◽  
Ethyl Acetate Fraction ◽  
Sepsis Model ◽  
Moringa Oleifera Leaves ◽  
Endothelial Stress

scholarly journals SKRINING SENYAWA METABOLIT SEKUNDER DAN UJI AKTIVITAS ANTIOKSIDAN EKSTRAK ETILASETAT DAUN WEDUSAN (Eupatorium odoratum)

Molekul ◽  
2009 ◽  
Vol 4 (2) ◽  
pp. 94
Author(s):  
Purwati Purwati ◽  
Undri Rastuti
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Research Method ◽  
Oxidation Process ◽  
Ethyl Acetate Extract ◽  
Natural Process ◽  
Inhibition Activity ◽  
Octadecanoic Acid ◽  
Fat Quality

Oxidation process is a natural process which always occurs in fat. The process affects and decreases the fat quality. Oxidation in fat can be hampered by the addition of antioxidant. Antioxidant activity of wedusan leaf has to be studied to know the possibility of wedusan leaf as an antioxidant. Hence, the aims of the research were to determine the antioxidant activity of ethyl acetate extract of wedusan leaf using TBA method, and to compare the antioxidant activity of wedusan leaf and that of BHT. The research method consisted of sample preparation, extraction, and determination of antioxidant activity using TBA method. Wedusan leaf was extracted by maceration using n-hexane and ethyl acetate solvents. The n-hexane extract was 2.90 gram, whereas ethyl acetate extract was 13.12 gram. Based on qualitative screening on secondary metabolites, ethyl acetate extract contained flavonoid. The results from GC-MS indicated that ethyl acetate extract contained methyl heptadecanoic, methyl-13-octadecenoic, 14,16-octadecadienal, and octadecanoic acid. The order of inhibition activity of antioxidant were 0.05% (w/v) of BHT > 0.15% (w/v) of ethyl acetate extract > 0.10% (w/v) of ethyl acetate extract > 0.05% (w/v) of ethyl acetate extract.

scholarly journals EVALUATION OF THE WOUND HEALING POTENTIAL OF OINTMENT PREPARATION OF ETHYL-ACETATE EXTRACT OF MORINGA OLEIFERA (LAM) IN RATS

2018 ◽  
Vol 15 (3) ◽  
pp. 64
Author(s):  
Morenike Coker ◽  
Grace Adejo ◽  
Benjamin Emikpe ◽  
Victor Oyebanji
Keyword(s):  
Wound Healing ◽  
Ethyl Acetate ◽  
Moringa Oleifera ◽  
Wound Closure ◽  
Ethyl Acetate Extract ◽  
Antibacterial Property ◽  
Closure Rate ◽  
Prolonged Contact ◽  
Ointment Base

Background: This study examined the in vitro antibacterial property of extracts of Moringa oleifera and the effect of different concentrations of the ethyl-acetate extract on cutaneous wound using an ointment delivery base. The aim of the study was to screen the extracts with best antibacterial property and evaluate effectiveness of different concentrations of the best extract when delivery is modified to ensure prolonged contact and reduced frequency of administration using an ointment base delivery vehicle. Materials and Methods: Dried and pulverized leaves of Moringa oleifera were screened for secondary metabolites. Successive gradient extraction was carried out using n-hexane, ethyl acetate and methanol. Leaf extracts were screened against clinical wound isolates of Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae and Proteus mirabilis using agar cup diffusion technique. The ethyl acetate extract was investigated for its healing efficiency on excision wound model in rats. The study made use of wistar rats (150-180 kg), randomized into Gentamicin, M. oleifera ointment (5% and 3.25%), and ointment base treatment groups. Treatments were applied topically on days 0, 7 and 14. The percentage wound closure rate was measured and histopathology of the healed wounds carried out. Results: In vitro antimicrobial screening showed that ethyl-acetate extract was effective against the test isolates. Topical application of ointment with 3.25% of the plant extract resulted in faster wound closure rate, rapid epithelization, resolution of granulation tissue, and remodeling at histology. Conclusion: This may be due to less interference by components of M. oleifera which appear to retard wound healing at higher concentrations. Therefore, M. oleifera 3.25% ointment preparation is recommended topically for wound healing.

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