scholarly journals ). EVALUATION OF THE WOUND HEALING POTENTIAL OF OINTMENT PREPARATION OF ETHYL-ACETATE EXTRACT OF MORINGA OLEIFERA (LAM) IN RATS

2018 ◽  
Vol 15 (3) ◽  
pp. 64-71
Author(s):  
Morenike Coker ◽  
Grace Adejo ◽  
Benjamin Emikpe ◽  
Victor Oyebamiji
Keyword(s):  
Wound Healing ◽  
Ethyl Acetate ◽  
Moringa Oleifera ◽  
Ethyl Acetate Extract ◽  
Moringa Oleifera Lam

scholarly journals EVALUATION OF THE WOUND HEALING POTENTIAL OF OINTMENT PREPARATION OF ETHYL-ACETATE EXTRACT OF MORINGA OLEIFERA (LAM) IN RATS

2018 ◽  
Vol 15 (3) ◽  
pp. 64
Author(s):  
Morenike Coker ◽  
Grace Adejo ◽  
Benjamin Emikpe ◽  
Victor Oyebanji
Keyword(s):  
Wound Healing ◽  
Ethyl Acetate ◽  
Moringa Oleifera ◽  
Wound Closure ◽  
Ethyl Acetate Extract ◽  
Antibacterial Property ◽  
Closure Rate ◽  
Prolonged Contact ◽  
Ointment Base

Background: This study examined the in vitro antibacterial property of extracts of Moringa oleifera and the effect of different concentrations of the ethyl-acetate extract on cutaneous wound using an ointment delivery base. The aim of the study was to screen the extracts with best antibacterial property and evaluate effectiveness of different concentrations of the best extract when delivery is modified to ensure prolonged contact and reduced frequency of administration using an ointment base delivery vehicle. Materials and Methods: Dried and pulverized leaves of Moringa oleifera were screened for secondary metabolites. Successive gradient extraction was carried out using n-hexane, ethyl acetate and methanol. Leaf extracts were screened against clinical wound isolates of Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae and Proteus mirabilis using agar cup diffusion technique. The ethyl acetate extract was investigated for its healing efficiency on excision wound model in rats. The study made use of wistar rats (150-180 kg), randomized into Gentamicin, M. oleifera ointment (5% and 3.25%), and ointment base treatment groups. Treatments were applied topically on days 0, 7 and 14. The percentage wound closure rate was measured and histopathology of the healed wounds carried out. Results: In vitro antimicrobial screening showed that ethyl-acetate extract was effective against the test isolates. Topical application of ointment with 3.25% of the plant extract resulted in faster wound closure rate, rapid epithelization, resolution of granulation tissue, and remodeling at histology. Conclusion: This may be due to less interference by components of M. oleifera which appear to retard wound healing at higher concentrations. Therefore, M. oleifera 3.25% ointment preparation is recommended topically for wound healing.

scholarly journals Antimicrobial Activity and Phytochemical Analysis of Moringa oleifera Lam. Crude Extracts Against Selected Bacterial and Fungal Strains

2018 ◽  
Vol 10 (02) ◽  
Author(s):  
Patel N ◽  
Mohan J S S
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Moringa Oleifera ◽  
Ethyl Acetate Extract ◽  
Micrococcus Luteus ◽  
Dilution Method ◽  
Phytochemical Analysis ◽  
Distilled Water ◽  
Bacterial Strains ◽  
Fungal Strains

The n- Hexane, ethyl acetate, methanol and distilled water Leaf, Stem, Flower and Fruit extracts of Moringa oleifera were evaluated for their antibacterial activity against six Gram-Negative and Gram-Positive bacteria and antifungal activity against seven fungal strains using Ciprofloxacin, Doxycycline, Fluconazole and Ketacozole as positive control. The activity was analyzed by well diffusion and two-fold serial broth dilution method of different extract. The study revealed that all the extracts irrespective of their types, in different concentrations inhibited growth of the test pathogens to varying degrees. Ethyl acetate extract showed maximum activity against all the bacterial strains followed in descending order by methanol, n-.Hexane and distilled water extracts. Ethyl acetate extract showed high antibacterial activity against Serratia marcescens (22mm) Methanol and n-Hexane extract were effective against Enterococcus faecalis and Bacillus subtilis (10mm) respectively. Aqueous extract showed maximum number of inhibition against Staphylococcus aureus (27mm) and Micrococcus luteus (18mm). Ethyl acetate extract showed maximum inhibition against Trichoderma harzianum (16mm) than other extracts were ineffective against selected fungus. MIC values were recorded as 0.125 to 4mg/ml. The phytochemical screening revealed the presence of phenols and flavonoids. Expression to these results it may be concluded that M. oleifera may be a potential source for the curing of various infectious diseases caused by the resistant microbes

scholarly journals Antibacterial and antifungal activity of Moringa oleifera stem bark

2013 ◽  
pp. 109-117 ◽  
Author(s):  
Md Shafiqur Rahman ◽  
Laila Zerin ◽  
MN Anwar
Keyword(s):  
Antifungal Activity ◽  
Ethyl Acetate ◽  
Pathogenic Bacteria ◽  
Moringa Oleifera ◽  
Ethyl Acetate Extract ◽  
Pathogenic Fungi ◽  
Stem Bark ◽  
Shigella Dysenteriae ◽  
Antibacterial And Antifungal Activity ◽  
Antibacterial And Antifungal

Petroleum ether, chloroform, ethyl acetate and carbon tetrachloride extracts of Moringa oleifera stem bark were studied for their antimicrobial activities against eleven human pathogenic bacteria (Shigella dysenteriae, S. sonnei, Salmonella typhi, S. paratyphi, Bacillus subtilis, B. megaterium, B. cereus, Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli and Vibrio cholerae) and four human pathogenic fungi (Aspergillus niger, A. ochraceus, A. ustus and Candida albicans) using disc diffusion and poisoned food method, respectively. Chloroform and ethyl acetate extracts exhibited moderate to good antibacterial and antifungal activity against all the pathogens tested. The ethyl acetate extract exhibited the largest zone of inhibition (21 mm in diameter with 2000 mg/disc extract) against S. sonnei. The highest inhibition of fungal radial mycelial growth (52. 00% with 100 mg extract/ml medium) was recorded against C. albicans with ethyl acetate extract. The ethyl acetate extract exhibited the lowest MIC (750 mg/ml) against B. megaterium, S. dysenteriae, V. cholerae and E. coli. For fungi, the lowest MIC was 500 mg/ml against C. albicans with crude extract of ethyl acetate. DOI: http://dx.doi.org/10.3329/cujbs.v3i1.13411 The Chittagong Univ. J. B. Sci.,Vol. 3(1&2):109-117, 2008

scholarly journals Antimicrobial evaluation of leaves of Balanites aegyptiaca (Linn.) Delile. & Moringa oleifera Lam.

2016 ◽  
Vol 7 (3) ◽  
Author(s):  
Malvika Saini ◽  
Tarun Sharma ◽  
Gaurav Sharma ◽  
Mita Kotecha
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Wound Healing ◽  
Moringa Oleifera ◽  
Activity Index ◽  
Diffusion Method ◽  
Klebsiella Aerogenes ◽  
Balanites Aegyptiaca ◽  
Healing Property ◽  
Moringa Oleifera Lam

Background : Balanites aegyptiaca (Linn.) Delile. & Moringa oleifera Lam. are well known drugs in Ayurveda for their wound healing property as they possess krimighna action or ability to kill foreign pathogens. Ingudi & Shigru leaves are used for wound healing purpose. Therefore leaves of both the plants were selected for antimicrobial study against surgical wound infection causative organisms. Methods: Both aqueous and alcoholic extracts of leaves of both plants at 10, 20, 30% concentrations were tested for antimicrobial activity by agar well diffusion method against a range of gram-positive and gram-negative bacteria. Zone of inhibition of extracts were determined, then activity index & IC50 value was calculated. Results: Both ethanol and aqueous extracts of  Balanites aegyptiaca (Linn.) Delile. & Moringa oleifera Lam. inhibited the growth of all the tested strains of bacteria. Aqueous extract of Balanites aegyptiaca (Linn.) Delile has more potent action against microorganism Staphylococcus aureus, Escherichia coli, Klebsiella aerogenes, ZOI (15mm,11mm,19mm respectively), AI(0.6, 0.61, 0.70  resp.), IC50 (1.778, 1.586, 1.196 resp.). Conclusion: Leaves of Balanites aegyptiaca (Linn.) Delile possess good antimicrobial effects against microorganism Staphylococcus aureus, Escherichia coli, Klebsiella aerogenes compared to Moringa oleifera Lam. Efficacy is very much significant against Klebsiella aerogenes. 

scholarly journals Evaluation of antioxidant, antibacterial and wound healing activities of Vitex pinnata

F1000Research ◽  
2020 ◽  
Vol 9 ◽  
pp. 187
Author(s):  
Nurul Ashifah Shafie ◽  
Nur Atiqah Suhaili ◽  
Hussein Taha ◽  
Norhayati Ahmad
Keyword(s):  
Wound Healing ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Radical Scavenging ◽  
Antibacterial Activities ◽  
Wound Model ◽  
Methanolic Extracts ◽  
Diffusion Assay ◽  
Dpph Radical Scavenging ◽  
Wound Healing Activity

Background: Vitex pinnata is a popular ethnomedicinal plant but scientific studies to validate its pharmacological properties are lacking for this plant. This study aims to determine the antioxidant, antimicrobial and wound healing properties of the methanolic extract of the leaves and the hexane, chloroform and ethyl acetate fractions.    Methods: The leaves of Vitex pinnata underwent methanol extraction and the methanol extract was fractionated with hexane, chloroform and ethyl acetate solvents. The antioxidant activity was determined using the DPPH radical scavenging assay. The antimicrobial activity was assessed by disc diffusion assay against Staphylococcus aureus, Bacillus subtilis, Escherichia coli and Pseudomonas aeruginosa. For the wound healing studies, the methanolic extracts of V. pinnata were used to prepare ointments with compositions of 10% (w/w) and 50% (w/w), which were evaluated for wound healing activity in an excision wound model in Wistar rats. Results: All the extracts showed antioxidant activities, with the ethyl acetate extract having the highest DPPH radical scavenging activity, followed by the methanol, chloroform and hexane extracts. Similarly, their quercetin equivalent concentrations were 33.1, 31, 20.3 and 4.5 mg/mL, respectively. Except for the methanol extract, the disc diffusion assay showed that the extracts demonstrated species-specific antibacterial activities, with the ethyl acetate extract showing antibacterial activities against all four tested strains. The wound healing activity of the high dose treated group (50% [w/w]) shows significant increase of wound contraction when compared to the control group. Conclusion: In the current study, the ethyl acetate extract showed activity for all tested bacteria and also had the highest DPPH activity. The methanolic extracts of V. pinnata leaves show modest wound healing activity in an excision wound model.

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