Short Communication: Variation in response to processing, in vitro gas production and fermentation of western Canadian feed barley

2014 ◽  
Vol 94 (4) ◽  
pp. 725-729 ◽  
Author(s):  
W. Z. Yang ◽  
M. Oba ◽  
M. L. Swift ◽  
T. A. McAllister

Yang, W. Z., Oba, M., Swift, M. L. and McAllister, T. A. 2014. Short Communication: Variation in response to processing, in vitro gas production and fermentation of western Canadian feed barley. Can. J. Anim. Sci. 94: 725–729. Sixty feed barley samples were collected from various locations in western Canada. Samples were either ground (1 mm) or dry-rolled to a processing index of 80%, and fermentability was assessed by measuring gas production (GP) and dry matter disappearances (DMD) at 0, 4, 8, 14 and 24 h of incubation using a batch culture technique. Physical and chemical composition, and GP and DMD varied substantially among samples. There were significant correlations among test weight and nutrient content of barley with GP and DMD of ground barley. The results indicate that the impact of nutrient content on in vitro DMD is more easily detected with ground than rolled barley.

Animals ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 1957
Author(s):  
Margarita Novoa-Garrido ◽  
Carlos Navarro Marcos ◽  
María Dolores Carro Travieso ◽  
Eduarda Molina Alcaide ◽  
Mogens Larsen ◽  
...  

The study analyzed the characteristics, chemical composition, and in vitro gas production kinetics of Porphyra umbilicalis and Saccharina latissima silages. Each seaweed was ensiled in vacuum bags (three bags/silage) following a 2 × 3 factorial design, with two pre-treatments (unwilted or pre-wilted) and three silage types: unwashed seaweed ensiled without additive; seaweed washed and ensiled without additive; and seaweed washed and ensiled with 4 g of formic acid (FAC) per kg seaweed. Silages were kept for 3 months in darkness at 20 °C. Pre-wilting prevented (p < 0.001) effluent formation and reduced (p ≤ 0.038) the production of NH3-N and volatile fatty acids for both seaweeds. Both pre-wilting and washing increased (p < 0.05) the ruminal degradability of P. umbilicalis silages but not of S. latissima silages. The pH of the FAC-treated silages was below 4.0, but ranged from 4.54 to 6.23 in non FAC-treated silages. DL-lactate concentrations were low (≤23.0 g/kg dry matter) and acetate was the predominant fermentation product, indicating a non-lactic fermentation. The estimated ruminal degradability of the P. umbilicalis and S. latissima silages was as average, 59.9 and 86.1% of that for high-quality rye-grass silages, respectively, indicating a medium-low nutritional value of these seaweed silages for ruminants.


Author(s):  
D. T. Q. Carvalho ◽  
A. R. F. Lucena ◽  
T. V. C. Nascimento ◽  
L. M. L. Moura ◽  
P. D. R. Marcelino ◽  
...  

Abstract The objective was to evaluate the fermentation profile, in vitro gas production and nutritional quality of pornunça (Manihot spp.) silages containing levels of condensed tannin (CT; 0, 4, 8 and 12% on dry matter (DM) basis), at five opening times (0, 3, 7, 14, 28 and 56 days). A completely randomized design in a 4 × 5 factorial arrangement was adopted, with four replications, totalling 80 experimental silos. The pH and NH3-N analyses were performed at all opening times of the silos. The other analyses were performed only with silages opened at 56 days of storage. There was an interaction effect between CT levels and silo opening times for pH and NH3-N. Tannin levels in pornunça silages after 56 days ensiling increased the pH and DM and reduced crude protein (CP) and neutral detergent fibre (NDF). There was a quadratic effect for NH3-N, acetic acid, butyric acid, gas losses, dry matter recovery (DMR), hemicellulose and acid detergent fibre. Inclusion of 4 and 8% CT in pornunça silage promotes a rapid decline in pH, being within the acceptable limit for adequate fermentation at 3 days of ensiling. Silages with 4% CT establish the pH at 28 days of opening the silos, with reduced NH3-N. Silages with 4% CT present higher concentrations of acetic and butyric acids and greater DMR. Inclusion of CT in pornunça silage after 56 days ensiling increases DM and reduces CP and NDF, directly affecting the in vitro degradability and reducing gas production.


Author(s):  
N.D. Meads ◽  
R. Tahmasbi ◽  
N. Jantasila

Greenhouse gas (GHG) emissions from livestock are an important consideration in environmental science. Estimating GHG production can be problematic at a farm or animal level, and requires controlled conditions to produce real data. An in vitro gas production technique (IVGPT) was developed to evaluate forage-based total mixed rations in digestion kinetics and GHG production. Two hundred and sixty samples of complete mixed rations (MR), which included a pasture component used in commercial lactating dairy herds, were collected around NZ across three calendar years, 2017-2019. Twenty of the 260 samples were 100% total mixed rations (TMR) with no pasture content. The samples were submitted for proximate analysis as well as IVGPT to generate GHG production figures. The results showed an average total gas production (TGP) of 129.82 ml/g dry matter (DM), 78.6% true digestibility (TDMD), 125.06 mg/g DM microbial biomass (MB), 20.16 g CH4/kg DM, and 12.8 MJME/kg DM. The average nutrient composition was dry matter (DM) 31.55%, crude protein (CP) 21.85%, neutral detergent fibre (NDF) 44.35%, and starch 7.03%. The IVGPT CH4 production was negatively correlated to NDF (r=-0.312), ADF (r=-0.193), TGP (r=-0.216), and was positively correlated with TDMD (r=0.250), apparent digestibility (ADMD) (r=0.614), starch (r=0.117) and volatile fatty acids (r=0.538). The MR diet showed a strong positive relationship with ADMD digestibility (P=0.01) and a negative relationship with fibre content (NDF, P=0.01 and ADF, P=0.01). However, CH4 production reduced linearly with increasing TGP (P=0.01). The results indicated that a greater CH4 production may be related to higher digestibility of mixed ration.


2012 ◽  
Vol 63 (2) ◽  
pp. 541-545 ◽  
Author(s):  
Sanjay Kumar ◽  
Sumit Singh Dagar ◽  
Sunil Kumar Sirohi ◽  
Ramesh Chandra Upadhyay ◽  
Anil Kumar Puniya

1997 ◽  
Vol 77 (5) ◽  
pp. 757-768 ◽  
Author(s):  
M. Blümmel ◽  
K. Becker

Fifty-four roughages of known voluntary dry-matter intakes (DMI; range 7·8−35·2 g/kg live weight per d) were examined in vitro in a gas production test. Samples (200 mg) of roughage and roughage neutral-detergent fibre (NDF) respectively were incubated in a mixed suspension of rumen contents for 96 h and the gas volumes recorded after 4,6,8,12,24,30,36,48,54,60 and 96 h. The kinetics of gas production were derived from the volume recordings described by the exponential equation Y=A+B(l—e-ct) where A is the intercept and ideally reflects the fermentation of the soluble and readily available fraction of the feed, B describes the fermentation of the insoluble (but with time fermentable) fraction and c the fractional rate at which B is fermented per h; A+B describes total fermentation. In vitro true dry matter (TD) and NDF degradabilities (NDF-D) after 24 h incubation were also determined. Of the variation in DMI, 75% was accounted for by the in vitro gas production parameters A, B and c in stepwise multiple regressions; 82% of the variation in DMI was explained by the parameters (ANDF+BNDF) and cNDF as obtained from the incubation of roughage NDF. The rate constants (c) were less important than parameters related to the extent of gas production, accounting for only 6·5 (whole roughage) and 4·1% (NDF) of the variation in DMI. There was no statistical advantage in the use of the exponential model describing extent and rate of fermentation over some of the simple gas volume measurements: 75% of the variation in DMI was accounted for by in vitro gas production of whole roughage after 8 h of incubation. On average gas production from NDF measured from 24–96 h accounted for 81% of the variation in DMI. A combination of gas volume measurements after a short period of incubation (4–8 h) with a concomitant determination of NDF-D after many hours (≥24 h) can render NDF preparations and long incubation times redundant. A method is suggested to obtain two results for DMI prediction in one single incubation. Of the variation in DMI 80% was accounted for by the incubation of 500 mg whole roughage when incubation was terminated after 24 h and the residual undegraded substrate quantified.


2006 ◽  
Vol 66 (1-3) ◽  
pp. 76-84 ◽  
Author(s):  
M.A. Cerrillo ◽  
O.O. López ◽  
C.G. Nevárez ◽  
R.G. Ramírez ◽  
R.A.S. Juárez

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