scholarly journals EFFECT OF AMOUNT AND LENGTH OF ALFALFA HAY IN DIETS CONTAINING BARLEY OR CORN ON SITE OF DIGESTION AND RUMEN MICROBIAL PROTEIN SYNTHESIS IN DAIRY COWS

1988 ◽  
Vol 68 (2) ◽  
pp. 445-454 ◽  
Author(s):  
L. M. RODE ◽  
L. D. SATTER
Keyword(s):  
Protein Synthesis ◽  
Turnover Rate ◽  
Block Design ◽  
Microbial Protein ◽  
Microbial Protein Synthesis ◽  
Alfalfa Hay ◽  
Total Digestion ◽  
Randomized Complete Block Design ◽  
Main Effects ◽  
Microbial N

Eight lactating Holstein cows fitted with simple cannulae in the rumen and proximal duodenum were used in a 2 × 2 × 2 factorial experiment in a randomized complete block design. The factors studied were: (1) forage length (full bloom alfalfa hay, long or chopped); (2) forage level in the total ration (25 or 75%); and (3) type of grain in the concentrate mixes (barley or corn). Organic matter (OM) intake was not affected by level of forage or grain type but was greater (P < 0.05) with chopped hay than with long hay. Apparent total digestion of OM increased (P < 0.01) from 59.7 to 68.1% as forage in the diet decreased from 75 to 25%, but site of digestion was unaffected. Flow to the duodenum of nonammonia-N, feed N, and microbial N were unaffected by forage level, forage length, or grain type. No differences in main effects were observed between corn and barley but interactions between grain type and forage length or amount indicate that type of grain had an influence upon site of OM digestion and flow of nonammonia-N and microbial N from the rumen. Efficiency of microbial protein synthesis (MPS) was positively associated with turnover rate of rumen solids regardless of liquid dilution rate. When no change in turnover rate of solids occurred, efficiency of microbial protein synthesis increased in association with increases in turnover rate of liquid. Key words: Lactation, cattle, bacteria, alfalfa, duodenum, rumen

scholarly journals EFFECT OF FORAGE AMOUNT AND PARTICLE SIZE IN DIETS OF LACTATING DAIRY COWS ON SITE OF DIGESTION AND MICROBIAL PROTEIN SYNTHESIS

1985 ◽  
Vol 65 (1) ◽  
pp. 101-111 ◽  
Author(s):  
L. M. RODE ◽  
D. C. WEAKLEY ◽  
L. D. SATTER
Keyword(s):  
Particle Size ◽  
Protein Synthesis ◽  
Dry Matter ◽  
Turnover Rate ◽  
Microbial Protein ◽  
Acid Detergent Fiber ◽  
Microbial Protein Synthesis ◽  
Lactating Dairy Cows ◽  
Alfalfa Hay ◽  
Concentrate Level

Lactating Holstein cows fitted with ruminal and duodenal T-type cannulae were used in two studies to determine the effect of forage: concentrate ratio and forage particle size on site of nutrient digestion and microbial protein synthesis. In exp. 1, cows were fed alfalfa hay at 24, 38, 58 and 80% of total dry matter intake. Organic matter (OM) digestion in the total tract (OMD) increased with increasing concentrate level but apparent ruminal OM digestion (AROMD) was unaffected by diet. Digestion of acid detergent fiber (ADF) was similar among the higher forage diets, but was depressed at the 24% forage level. Efficiency of microbial protein synthesis (MPS) was depressed by high concentrate diets and was positively correlated (P < 0.05) to turnover rate within the rumen. In exp. 2, cows were fed 20% concentrate and 80% alfalfa hay in long, chopped or ground and pelleted form. AROMD was lower with ground hay but OMD was unaffected by diet. Digestibility of ADF in the rumen was lower with ground hay, but was partially compensated for by increased hindgut digestion of ADF. Flow of feed plus endogenous nitrogen (N) at the duodenum was 37% and 47% on N intake with long and ground hay, respectively. Efficiency of MPS increased 15% and postruminal N digestion increased 36% when ground hay replaced long hay. Efficiency of MPS was directly related to ruminal solids turnover rate and inversely related to liquid dilution rate. These results demonstrate improvements in efficiency of MPS with either increasing amounts of forage in the diet or increased ruminal passage of solids. Key words: Digestion, microbial protein, rate of passage, cattle, rumen forage

scholarly journals Effect of urea coating by chitosan on the dynamics of ammonia concentration and rumen fermentation in vitro

2022 ◽  
Vol 951 (1) ◽  
pp. 012004
Author(s):  
S Nayohan ◽  
K G Wiryawan ◽  
A Jayanegara
Keyword(s):  
Protein Synthesis ◽  
Organic Matter ◽  
Dry Matter ◽  
Block Design ◽  
Ammonia Concentration ◽  
Rumen Fermentation ◽  
Microbial Protein ◽  
Microbial Protein Synthesis ◽  
Randomized Complete Block Design

Abstract The aim of this study was to determine the effect of coating urea by chitosan at graded levels on ammonia concentration and rumen fermentation in vitro. This study used Factorial Randomized Complete Block Design (RCBD) to test ammonia parameter and Randomized Complete Block Design (RCBD) for pH, microbial protein synthesis, dry matter and organic matter digestibility, and Volatile Fatty Acid (VFA). The treatments tested were: P0 = addition non coating urea 1%; P1 = coating urea by chitosan 1%; P2 = coating urea by chitosan 2%; P3 = coating urea by chitosan 3%. The data obtained were analysed by using ANOVA and continued with Tukey HSD test with SPSS version 25. The results of this study showed that the coating of urea chitosan had no significant effect on pH, dry matter and organic matter digestibility, microbial protein synthesis, and amonia concentration in the rumen. However, it significantly reduced (P <0.05) total VFA concentration. It can be concluded that the application of urea coating by chitosan does not affect on the degradation of urea in the rumen.

scholarly journals PSXI-15 Effects of post-pyrolysis treated biochars on nutrient disappearance, methane production and ruminal fermentation of a silage-based diet in an artificial rumen system (RUSITEC)

2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 395-395
Author(s):  
Paul Tamayao ◽  
Gabriel O Ribeiro ◽  
Tim A McAllister ◽  
Hee-Eun Yang ◽  
A M Saleem ◽  
...  
Keyword(s):  
Protein Synthesis ◽  
Block Design ◽  
Gas Production ◽  
Neutral Detergent Fiber ◽  
Canola Meal ◽  
Acid Mixture ◽  
Vitamin Supplement ◽  
Microbial Protein ◽  
Microbial Protein Synthesis ◽  
Barley Silage

Abstract This study investigated the effects of post-pyrolysis treated biochar on nutrient disappearance, total gas and methane (CH4) production, rumen fermentation and microbial protein synthesis in an artificial rumen system (RUSITEC) fed a barley silage-based diet. The basal diet consisted of 60% barley silage, 27% barley grain, 10% canola meal and 3% mineral/vitamin supplement (DM basis). Three spruced-based biochars, treated post-pyrolysis with either zinc chloride, hydrochloric acid/nitric acid mixture or sulfuric acid were added at 2.0% of substrate DM. In a randomized complete block design, treatments were assigned to sixteen vessels (n = 4/treatment) in two RUSITEC systems. The experiment was conducted over 15 d, with 8 d of adaptation and 7 d of sampling. Nutrient disappearance of dry matter (DM), organic matter (OM), acid detergent fiber (ADF) and neutral detergent fiber (NDF) was determined after 48 h of incubation from d 9 to 12, and microbial protein synthesis was measured from d 13–15. Data were analyzed using PROC MIXED in SAS, with the fixed effect of treatment and random effect of RUSITEC system and vessel. Biochar inclusion did not affect disappearance of DM (P = 0.49), OM (P = 0.60), CP (P = 0.14), NDF (P = 0.48), ADF (P = 0.11) or starch (P = 0.58). Biochar also had no effect on total gas production (P = 0.31) or CH4 produced expressed as a % of total gas production (P = 0.06), mg/d (P = 0.70), mg/g of DM incubated (P = 0.74), or mg/g of DM digested (P = 0.64). No effect on total VFA (P = 0.56) or NH3-N (P = 0.20) production were observed. Neither microbial protein synthesis nor total protozoa count were affected by biochar addition (P &gt; 0.05). In conclusion, biochar inclusion in a silage-based diet did not exhibit the potential to mitigate CH4 emissions or improve digestion in a RUSITEC system.

Effect of pine-based biochars with differing physiochemical properties on methane production, ruminal fermentation, and rumen microbiota in an artificial rumen (RUSITEC) fed barley silage

2021 ◽  
pp. 1-13
Author(s):  
Paul Tamayao ◽  
Gabriel O. Ribeiro ◽  
Tim A. McAllister ◽  
Kim H. Ominski ◽  
Atef M. Saleem ◽  
...  
Keyword(s):  
Protein Synthesis ◽  
Fixed Effects ◽  
Block Design ◽  
Rumen Fermentation ◽  
Ruminal Fermentation ◽  
Microbial Protein ◽  
Rumen Microbiota ◽  
Alpha And Beta Diversity ◽  
Microbial Protein Synthesis ◽  
Barley Silage

This study investigated the effects of three pine-based biochar products on nutrient disappearance, total gas and methane (CH4) production, rumen fermentation, microbial protein synthesis, and rumen microbiota in a rumen simulation technique (RUSITEC) fed a barley-silage-based total mixed ration (TMR). Treatments consisted of 10 g TMR supplemented with no biochar (control) and three different biochars (CP016, CP024, and CP028) included at 20 g·kg−1 DM. Treatments were assigned to 16 fermenters (n = 4 per treatment) in two RUSITEC units in a randomized block design for a 17 d experimental period. Data were analyzed using MIXED procedure in SAS, with treatment and day of sampling as fixed effects and RUSITEC unit and fermenters as random effects. Biochar did not affect nutrient disappearance (P > 0.05), nor total gas or CH4, irrespective of unit of expression. The volatile fatty acid, NH3-N, total protozoa, and microbial protein synthesis were not affected by biochar inclusion (P > 0.05). Alpha and beta diversity and rumen microbiota families were not affected by biochar inclusion (P > 0.05). In conclusion, biochar did not reduce CH4 emissions nor affect nutrient disappearance, rumen fermentation, microbial protein synthesis, or rumen microbiota in the RUSITEC.

scholarly journals Coffee hull in the diet of dairy heifers: nitrogen balance and microbial protein synthesis

2010 ◽  
Vol 39 (5) ◽  
pp. 1141-1145 ◽  
Author(s):  
Alexandre Lima de Souza ◽  
Rasmo Garcia ◽  
Luciano da Silva Cabral ◽  
Mara Lúcia Albuquerque Pereira ◽  
Rilene Ferreira Diniz Valadares
Keyword(s):  
Protein Synthesis ◽  
Nitrogen Balance ◽  
Dry Matter ◽  
Block Design ◽  
Nitrogen Excretion ◽  
Microbial Protein ◽  
Purine Derivative ◽  
Microbial Protein Synthesis ◽  
Random Block ◽  
Random Block Design

It was evaluated nitrogen compounds and microbial protein synthesis in heifers fed diets containing coffee hulls (0.0; 8.75; 17.25; and 26.25% of dry matter) replacing ground corn concentrate at the following levels of coffee hulls in the total diet dry matter: 0.0, 3.5, 7.0 or 10.5%. It was used 24 crossbreed heifers (7/8, 15/16 and 31/32 Holstein-Zebu), which were distributed in a random block design made up accordingly to the weight of the animals. Spot samples of urine were colleted aproximatelly four hours after morning feeding and were used to estimate microbial protein synthesis by using urine purine derivatives. It was not observed effect of coffee hull levels in the diet on total nitrogen intake (160 g/day) and nitrogen excretion in the urine (87.4 g/day). The inclusion of coffee hull in the diet linearly increased nitrogen excretion in feces, as well as nitrogen balance. There was linear reduction in urinary excretion of allantoin, in total purine derivative and absorbed purine, which reduced 0.715, 0.873, and 0.954 mmol/day to each coffee hull unity added to the concentrate, respectively. Coffee hull altered microbial protein synthesis, which reduced in 0.687 g/day to each coffee hull unity added to the concentrate. Reduction in microbial protein synthesis can reduce weight gain in heifers fed coffee hulls.

scholarly journals Dynamics of large ciliate protozoa in the rumen of cattle fed on diets of freshly cut grass

1986 ◽  
Vol 56 (2) ◽  
pp. 455-462 ◽  
Author(s):  
R. A. Leng ◽  
D. Dellow ◽  
G. Waghorn
Keyword(s):  
Protein Synthesis ◽  
Production Rate ◽  
Small Proportion ◽  
Turnover Rate ◽  
Turnover Time ◽  
Microbial Protein ◽  
Microbial Protein Synthesis ◽  
Lysis Rate ◽  
Ciliate Protozoa

1. The dynamics of large ciliate (holotrich) protozoa (Isotricha and Dasytricha spp.) in the rumen of cattle given cut, fresh ryegrass (Lolium multiflorium Lam) were studied by means of a single intrarumen injection of14C-labelled protozoa prepared in vitro by adding [Me14C]choline to rurnen fluid containing protozoa and incubating at 39° for 2 h.2. An indication of the lysis rate of protozoa in the rumen was obtained from the radioactivity apparently lost through the methane pool.3. The turnover time of the holotrich protozoa indicates that these protozoa were extensively retained in the rumen and that only a small proportion of those produced in the rumen flowed out in the digesta. This was supported by the estimation of the rate of lysis which was approximately 85% of the turnover rate in the rurnen.4. The apparent production rate of the larger protozoa indicates that they contribute only about 9% of the predicted net microbial protein synthesis in the rumen.

scholarly journals Quantitative studies of food protein degradation and the energetic efficiency of microbial protein synthesis in the rumen of sheep given chopped lucerne and rolled barley

1981 ◽  
Vol 45 (3) ◽  
pp. 587-604 ◽  
Author(s):  
J. C. Mathers ◽  
E. L. Miller
Keyword(s):  
Protein Synthesis ◽  
Rumen Fluid ◽  
Block Design ◽  
Ammonia Nitrogen ◽  
Energetic Efficiency ◽  
Food Protein ◽  
Microbial Protein ◽  
Microbial Protein Synthesis ◽  
Randomized Block Design ◽  
Outflow Rate

1. In a randomized block design, four sheep were given 800 g daily of diets containing: chopped lucerne (L), chopped lucerne–rolled barley (2:1; LB), rolled barley–chopped lucerne (2:1; BL), rolled barley (B); each diet was supplemented with minerals, vitamins and urea as considered necessary. Chromic oxide was included in the diets as a flow marker.2. Flows of organic matter (OM) and non-ammonia-nitrogen (NAN) to the small intestine (SI) were measured and microbial protein was identified by a35S-incorporation procedure.3. OM disappearance in the rumen increased linearly with increasing inclusion of barley in the diet but there was no significant change in microbial NAN flow to the SI so that the yield of microbial NAN (g)/kg fermented OM (FOM) decreased from 29.6 (diet L) to 22.7 (diet B). Changes in the energetic efficiency of microbial protein synthesis appeared to be unrelated to alterations in rumen fluid volatile fatty acid (VFA) proportions or in rumen fluid dilution rate (D).4. The degradability of dietary protein (non-urea-N), estimated using the35S procedure, was 0.72, 0.76, 0.86 and 0.86 for diets L, LB, BL and B respectively. Similar values were obtained from concurrent polyester-bag experiments when the fractional outflow rate of undergraded protein from the rumen (k) was assumed to be 0.046.

Effects of synchronizing the rate of dietary energy and nitrogen release in diets with a similar carbohydrate composition on rumen fermentation and microbial protein synthesis in sheep

1995 ◽  
Vol 124 (3) ◽  
pp. 463-472 ◽  
Author(s):  
L. A. Sinclair ◽  
P. C. Garnsworthy ◽  
J. R. Newbold ◽  
P. J. Buttery
Keyword(s):  
Protein Synthesis ◽  
Wheat Straw ◽  
Rumen Fermentation ◽  
Winter Barley ◽  
Dietary Energy ◽  
Carbohydrate Composition ◽  
Microbial Protein ◽  
Microbial Protein Synthesis ◽  
Cyclical Trend ◽  
Microbial N

SUMMARYThe effects of synchronizing dietary energy and nitrogen supply in diets with a similar carbohydrate composition on microbial protein synthesis and rumen fermentation were examined in sheep. Two diets were formulated to be either synchronous (diet S) or asynchronous (diet A) for the hourly release of nitrogen (N) and energy to the rumen. Diet S contained (g/kg) 425 g wheat straw, 400 g winter barley, 150 grapeseed meal and 25 g minerals/vitamins and diet A contained 505 g wheat straw, 458.5 g winter barley, 11·5 g urea and 25 g minerals/vitamins. Both diets were fed at the rate of 1 kg/day in four equal portions, to four cannulated sheep, in two periods in a change-over design. Rumen ammonia concentrations followed the predicted hourly trend in N degradation with a peak 1 h after feeding of 10 mM for diet S and 16 mM for diet A before falling within 3 h of feeding to 4 ITIM in animals fed either diet. Rumen volatile fatty acid (VFA) concentrations followed the cyclical trend predicted by stoichiometric equations, whilst rumen VFA ratios were more stable than predicted in animals fed either diet. The observed content of rumen degradable protein and organic matter truly degraded in the rumen was similar for both diets. The increase in total CHO digested in the rumen observed with diet A (427 g/kg DM) compared with diet S (364 g/kg DM) can be attributed to the greater content of starch in the asynchronous diet, which had a high degradability. The efficiency of microbial protein synthesis (g N/kg OM truly degraded in the rumen) was 11–20% greater in animals fed the synchronous diet (S) than the asynchronous diet (A). It is concluded that microbial N production was more efficient when dietary energy and N supply were synchronized.

scholarly journals IMBANGAN HIJAUAN DAN KONSENTRAT DALAM RANSUM TERHADAP RESPON FERMENTASI RUMEN DAN SINTESIS PROTEIN MIKROBA PEDET SAPI BALI CALON INDUK

2018 ◽  
Vol 21 (2) ◽  
pp. 60
Author(s):  
BUDIASA I K. M. ◽  
N. N. SURYANI ◽  
I W. SUARNA
Keyword(s):  
Protein Synthesis ◽  
Energy Supply ◽  
Block Design ◽  
Microbial Protein ◽  
Host Animal ◽  
Microbial Protein Synthesis ◽  
Randomized Block Design ◽  
Average Body ◽  
Average Body Weight

In order to fulfill the domestic meat requirements and reduce imports so government continually develop localbeef cattle based on their quantity and quality of meat. Among the local cattle in Indonesia, Bali cattle is the mostfavourite cattle (32.31%) considering on its meat quality. There were 12 calves used in this research with 100 kgof average body weight. The treatments were diet with 40% concentrate and 60% king grass (A); 45% concentrateand 55% king grass (B); 50% concentrate and 50% king grass (C); 55% concentrate and 45% king grass (D). Theexperiment was design in a completely randomized block design with four treatments and three replicates. Thevariables measured were rumen fermentation as of pH, NH3, total and pastial of VFA (acetic acid, propionic acidand butyric acid), microbial protein synthesis and protozoa populations. It showed that treatment D produce thehighest NH3 and total VFA, 14.66 mMol, and 165.38 mMol respectively compared to other treatments. Propionicacid was 27.87 mMol the highest in treatment D and 368.62 g/h/d microbial protein synthesis. It can be concludedthe higher proportion of concentrate in the bali heifer calves so the more energy supply, in this case, SPM availabilityfor the host animal is higher.

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