Intake, digestibility and aerobic stability of barley silage inoculated with mixtures of Lactobacillus plantarum and Enterococcus faecium

1995 ◽  
Vol 75 (3) ◽  
pp. 425-432 ◽  
Author(s):  
T. A. McAllister ◽  
L. B. Selinger ◽  
L. R. McMahon ◽  
H. D. Bae ◽  
T. J. Lysyk ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactobacillus Plantarum ◽  
Enterococcus Faecium ◽  
Volatile Fatty Acids ◽  
Average Daily Gain ◽  
Water Soluble ◽  
Neutral Detergent Fiber ◽  
Soluble Carbohydrates ◽  
Aerobic Stability ◽  
Barley Silage

The effect of ensiling barley treated with two bacterial inoculants containing mixtures of Lactobacillus plantarum and Enterococcus faecium (1.0 × 105 cfu g−1 as fed silage) on the nutritional value and aerobic stability of barley silage was examined. Inoculants differed in the strains they contained and were originally selected by Pioneer Hi-Bred International for use with corn or alfalfa silage, SILA-BAC® (1174), or with grass silage (X2637). Concentrations of water-soluble carbohydrates were higher (P < 0.05) in inoculated than in control silages. Although inoculants appeared to increase the numbers of lactic acid producing bacteria (LAB) at ensiling, post-ensiling numbers (cfu g−1) of yeasts and molds were lower (P < 0.05) in inoculated than in control silages. Lactic acid concentrations and pH were similar among the silages and variations m the growth of yeast and mold populations could not be explained by differences in the production of volatile fatty acids (VFA) among silages. Inoculation of barley silage with either inoculant increased (P < 0.01) the average daily gain of lambs. A digestibility experiment with 12 growing ram lambs showed that inoculants did not alter (P > 0.05) DM intake, feed efficiency or the digestion of DM, organic matter, acid detergent fiber (ADF) and neutral detergent fiber (NDF). Nitrogen intake and retention were greater (P < 0.05) in lambs fed silage inoculated with 1174 as compared with control silage. Yeast populations were increased (P < 0.05) in control and 1174 after 2 d of exposure to air but it required 13 d for a similar yeast population to be established in X2637 silage. Increases in the mold populations within the silages were noted after 2, 5 and 13 d of exposure to air for control, 1174 and X2637, respectively. The temperature of control silage increased (P < 0.05) 2 d after exposure to air, whereas increases in temperature were delayed for 4 d in 1174 and 8 d in X2637. Temperatures rose as high as 30 °C in control silage, but did not exceed 24 °C in inoculated silages during the 13 d period. Key words: Barley silage, inoculant, digestion, aerobic stability, sheep, gain

scholarly journals Effects of Class IIa Bacteriocin-Producing Lactobacillus Species on Fermentation Quality and Aerobic Stability of Alfalfa Silage

Animals ◽  
2020 ◽  
Vol 10 (9) ◽  
pp. 1575
Author(s):  
Fuhou Li ◽  
Zitong Ding ◽  
Adegbola T. Adesogan ◽  
Wencan Ke ◽  
Yun Jiang ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactobacillus Plantarum ◽  
Crude Protein ◽  
Ammonia Nitrogen ◽  
Water Soluble ◽  
Neutral Detergent Fiber ◽  
Lactobacillus Delbrueckii ◽  
Aerobic Stability ◽  
Fermentation Quality ◽  
Alfalfa Silage

The effects of two strains of class IIa bacteriocin-producing lactic acid bacteria, Lactobacillus delbrueckii F17 and Lactobacillus plantarum (BNCC 336943), or a non-bacteriocin Lactobacillus plantarum MTD/1 (NCIMB 40027), on fermentation quality, microbial counts, and aerobic stability of alfalfa silage were investigated. Alfalfa was harvested at the initial flowering stage, wilted to a dry matter concentration of approximately 32%, and chopped to 1 to 2 cm length. Chopped samples were treated with nothing (control, CON), Lactobacillus delbrueckii F17 (F17), Lactobacillus plantarum (BNCC 336943) (LPB), or Lactobacillus plantarum MTD/1 (NCIMB 40027) (LPN), each at an application rate of 1 × 106 colony-forming units/g of fresh weight. Each treatment was ensiled in quadruplicate in vacuum-sealed polyethylene bags packed with 500 g of fresh alfalfa per bag and ensiled at ambient temperature (25 ± 2 °C) for 3, 7, 14, 30, and 60 days. The samples were then subjected to an aerobic stability test after 60 days of ensiling. Compared with the CON silage, the inoculants reduced the pH after 14 days of ensiling. After 60 days, pH was lowest in the LPB-treated silage, followed by the F17 and LPN-treated silages. Inoculation of F17 increased concentrations of lactic acid in silages fermented for 7, 14, 30, and 60 days relative to other treatments, except for the LPN-treated silages ensiled for 30 and 60 days, in which the lactic acid concentrations were similar to that of F17 silage. Application of F17 and LPB decreased the number of yeast and mold relative to CON and LPN-treated silages. Compared with the CON silage, inoculant-treated silages had greater aerobic stability, water-soluble carbohydrate, and crude protein concentrations, and lower neutral detergent fiber, amino acid nitrogen, and ammonia nitrogen concentrations. The LPB-treated silage had the greatest aerobic stability followed by the F17-treated silage. Both class IIa bacteriocin producing inoculants improved alfalfa silage fermentation quality, reduced the growth of yeasts and molds, and improved the aerobic stability of the ensiled forage to a greater extent than the proven LPN inoculant. However, higher crude protein concentration and lower ammonia nitrogen concentration were observed in LPN-treated silage relative to other treatments.

scholarly journals Effects of additives on the fermentation quality, in vitro digestibility and aerobic stability of mulberry (Morus alba L.) leaves silage

2020 ◽  
Vol 33 (8) ◽  
pp. 1292-1300
Author(s):  
Zhihao Dong ◽  
Siran Wang ◽  
Jie Zhao ◽  
Junfeng Li ◽  
Tao Shao
Keyword(s):  
Lactic Acid ◽  
Lactobacillus Plantarum ◽  
Water Soluble ◽  
Soluble Carbohydrates ◽  
In Vitro Digestibility ◽  
Fresh Matter ◽  
Mulberry Leaves ◽  
Aerobic Stability ◽  
Fermentation Quality

Objective: To explore feed resources capable of replacing regular poor-quality fodder, this study was conducted to evaluate the effects of additives on the fermentation quality, <i>in vitro</i> digestibility and aerobic stability of mulberry leaves silage.Methods: The mulberry leaves were ensiled either untreated (control) or treated with 1×10<sup>6</sup> cfu/g fresh matter <i>Lactobacillus plantarum</i> (L), 1% glucose (G), 3% molasses (M), a combination of 1% glucose and <i>Lactobacillus plantarum</i> (L+G), and a combination of 3% molasses and <i>Lactobacillus plantarum</i> (L+M). The fermentation quality and chemical composition were analyzed after 7, 14, 30, and 60 d, respectively. The 60-d silages were subjected to an aerobic stability test and fermented with buffered rumen fluid to measure the digestibility.Results: Inoculating lactic acid bacteria (LAB) resulted in more rapid increase in lactic acid concentrations and decline in pH of mulberry leaves silage as compared control. Higher acetic acid and lower ethanol and ammonia nitrogen concentrations (p<0.05) were observed in the LAB-inoculated silages as opposed to control during ensiling. The LAB-inoculated silages contained lower water-soluble carbohydrates compared with control during the first 14 d of ensiling, and lower neutral detergent fibre (p<0.05) concentrations as compared with non-LAB inoculated silages. Adding molasses alone increased (p<0.05) the digestibility of dry matter (DM). The aerobic stability of mulberry leaves silage was increased by LAB inoculation, whereas decreased by adding glucose or molasses.Conclusion: The LAB inoculation improved fermentation quality and aerobic stability of mulberry leaves silage, while adding glucose or molasses failed to affect the fermentation and impaired the aerobic stability. Inoculating LAB alone is recommended for mulberry leaves especially when ensiled at a relatively high DM.

scholarly journals Application of some lactic acid bacteria strains to improve fermentation and aerobic stability of maize silage

2019 ◽  
Vol 7 (1-2) ◽  
pp. 127-132
Author(s):  
Judit Peter Szucs ◽  
Agnes Suli ◽  
Timea Suli Zakar ◽  
Elizabet Berecz ◽  
Mate Pek
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Lactobacillus Plantarum ◽  
Lactococcus Lactis ◽  
Enterococcus Faecium ◽  
Acid Production ◽  
Untreated Control ◽  
Raw Material ◽  
Aerobic Stability ◽  
Whole Plant

The object of the trial was to study the effect of some lactic acid bacteria strains on the fermentation and aerobic stability of whole plant maize silages.The whole plant maize raw material was 32% DM, in soft cheddar stage of grain ripeness. It was ensiled in 4.2 litre capacity glass micro-size silos in 5 replicates /each treatment and stored on constant air conditioned room temperature (22 oC) during 95 days. The average packing density of raw material was 211 kg DM/m3.The applied treatments: 1. Untreated control maize, 2. Enterococcus faecium 100,000 CFU/g fresh maize (FM), 3. Lactobacillus plantarum 50,000 CFU/g FM + Enterococcus faecium 50,000 CFU/g FM, 4. Lactococcus lactis 100,000 CFU/g FM, 5. Lactobacillus plantarum 50,000 CFU/g FM + Lactococcus lactis 50,000 CFU/g FM, 6. Lactobacillus plantarum 100,000 CFU/g FM.Aerobic stability study:  Applied Honig (1990 system).The main experiences are the following: Applied lactic acid bacteria strains improved the quality of fermentation of maize in general compare to untreated control one.Lactic acid bacteria strains significantly stimulated lactic acid production and decreased propionic and butyric acid production. The origin of ammonia decreased also under influence of lactic acid bacteria strains in unaerobic conditions.Enterococcus faecium and.Lactococcus lactis are not able to protect the maize silages against the aerobic deterioration with the applied dosage.  Lactobacillus plantarum itself produced the most favourable fermentation characteristics and protected the aerobic stability of silage the most effectively (during 4 day) compare to all other treated maize silages.

scholarly journals Fermentation quality and aerobic stability of Napier grass ensiled with citric acid residue and lactic acid bacteria

2021 ◽  
Vol 9 (1) ◽  
pp. 52-59
Author(s):  
Xuxiong Tao ◽  
Chongwen Ji ◽  
Sifan Chen ◽  
Jie Zhao ◽  
Siran Wang ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Lactic Acid ◽  
Citric Acid ◽  
Lactic Acid Bacteria ◽  
Water Soluble ◽  
Neutral Detergent Fiber ◽  
Napier Grass ◽  
Pennisetum Purpureum ◽  
Aerobic Stability ◽  
Fermentation Quality

This study was conducted to investigate the effects of adding citric acid residue (CAR) with or without lactic acid bacteria (LAB) to Napier grass (Cenchrus purpureus; syn. Pennisetum purpureum) cv. Sumu No. 2 at ensiling on the fermentation quality and aerobic stability of the resulting silage. Treatments included: Control (Napier grass forage without additives); and Napier grass inoculated with lactic acid bacteria (Lactobacillus plantarum and L. buchneri) at 1 × 106 cfu/gfresh weight (FW) forage (LAB) or 36 g citric acid residue/kg FW forage (CAR) or a mixture of CAR and LAB (CL). Forty-five days after ensiling the silages were tested for chemical and microbial composition and an aerobic stability test was conducted. The addition of CAR with or without LAB increased the DM and lactic acid concentrations in silage and decreased pH plus acetic acid, ammonia nitrogen (NH3-N), neutral detergent fiber and cellulose concentrations relative to Control. The pH in LAB silage was lower than in Control, while lactic acid concentration was higher. During the first 2 days of aerobic exposure, all additives increased the water-soluble carbohydrate (WSC) and lactic acid concentrations and decreased pH plus NH3-N and acetic acid concentrations. Moreover, CL silages had the highest WSC and the lowest NH3-N and acetic acid concentrations during aerobic exposure. However, all additives failed to improve the aerobic stability of the silage. While CAR with or without LAB inoculant improved the fermentation quality of silage made from Napier grass, more studies are warranted to identify additives which can improve aerobic stability of the silage after opening.

scholarly journals Propiyonik Asit Katkısının Toplam Karışım Rasyonu Yemin Aerobik Stabilite Özellikleri Üzerine Etkileri

2021 ◽  
Vol 9 (1) ◽  
pp. 210-216
Author(s):  
Ahmet Aslım ◽  
Berrin Okuyucu ◽  
Fisun Koç
Keyword(s):  
Lactic Acid ◽  
Propionic Acid ◽  
Storage Conditions ◽  
Water Soluble ◽  
Neutral Detergent Fiber ◽  
Soluble Carbohydrate ◽  
Total Mixed Ration ◽  
Aerobic Stability ◽  
Different Levels ◽  
Feed Samples

This study investigated the effects of different levels of propionic acid addition on the aerobic stability characteristics of the total mixture ration. In the study, the effects of four different levels of propionic acid-based additives (0, 1.5, 3.0, 4.5%) on storage conditions of 26°C and 30°C. Feed samples were stored for 7 days, 3 replicates for each treatment group. Chemical and microbiological parameters were analysed in feed samples during aerobic stability. Temperature values and ambient temperature in each treatment were measured and recorded through temperature sensors for 7 days. The addition of additives in the study decreased the pH, dry matter, neutral detergent fiber, and yeast contents of total mixed ration, increased crude protein, eter extract, water soluble carbohydrate, lactic acid, and lactic acid bacteria contents and prevented mold growth. As a result of the research, the addition of 4.5% propionic acid allowed the total mixed ration to remain stable at 26°C for 7 days and 30°C for 5 days.

Corrigendum to: Effect of Lactobacillus plantarum and Lactobacillus buchneri addition on fermentation, bacterial community and aerobic stability in lucerne silage

2019 ◽  
Vol 59 (8) ◽  
pp. 1584
Author(s):  
Huazhe Si ◽  
Hanlu Liu ◽  
Zhipeng Li ◽  
Weixiao Nan ◽  
Chunai Jin ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Microbial Community ◽  
Lactic Acid Bacteria ◽  
Lactobacillus Plantarum ◽  
Water Soluble ◽  
Metabolic Phenotype ◽  
Residual Water ◽  
Lactobacillus Buchneri ◽  
Silage Fermentation ◽  
Aerobic Stability

Changes in the microbial community are closely related to the fermentation of silage. However, how host genetic variation shapes the community structure of the silage microbiota and its metabolic phenotype is poorly understood. The objective of present study was to evaluate the effects of the application of the homo-fermentative Lactobacillus plantarum and hetero-fermentative Lactobacillus buchneri strains to lucerne silage on the fermentation characteristics, aerobic stability, and microbial community and their correlations. The three silages treated with L. plantarum or L. buchneri were well preserved and had significantly lower pH values, butyric acid, propionic acid, and ammonia-N concentrations, and significantly higher residual water-soluble carbohydrate, dry matter and lactic acid contents than the controls. The treated groups had more lactic acid bacteria and lower quantities of other bacteria in their microbial communities. Inoculation of lactic acid bacteria influenced the abundances of other bacteria and controlled the silage fermentation characteristics. L. buchneri inhibited the abundance of Enterobacter_ludwigii to increase the crude protein content, L. plantarum improve the neutral detergent fibre content by affecting the abundance of Arthrobacter_sp._Ens13. In conclusion, the application of L. plantarum and L. buchneri improved the quality of lucerne silage fermentation, and L. buchneri resulted in greater improvements after aerobic exposure.

Effects of dry-matter content on the nutritive value of individually wrapped round-bale timothy silage fed to sheep

1993 ◽  
Vol 73 (2) ◽  
pp. 343-354 ◽  
Author(s):  
R. Beaulieu ◽  
J. R. Seoane ◽  
P. Savoie ◽  
D. Tremblay ◽  
R. Thériault ◽  
...  
Keyword(s):  
Dry Matter ◽  
Nutritive Value ◽  
Average Daily Gain ◽  
Matter Content ◽  
Metabolizable Energy ◽  
Water Soluble ◽  
Neutral Detergent Fiber ◽  
Soluble Carbohydrates ◽  
Dry Matter Content ◽  
Chopped Grass

Thirty-two sheep (30.0 kg avg. BW) were used to study the effects of dry-matter (DM) content at harvest on the nutritive value of timothy grass silages conserved as round bales of high (52.1%, DM50), medium (39.9%, DM40) and low (23.1%, DM25) DM. Chopped grass of 24.4% DM conserved in a horizontal silo (HS) was used as a control. Gross energy, crude protein (CP) and acid detergent fibre (ADF) contents were similar for all silages (P > 0.05). Neutral detergent fiber (NDF) contents decreased as DM of the silages decreased (P < 0.05). Acid detergent lignin and ash contents were highest in HS and lowest in DM40 silages (P < 0.05). Silage pH and water-soluble carbohydrates decreased with decreasing DM of the silages (P < 0.05), while ammonia-N and lactate levels increased (P < 0.05). Acetate concentrations in round bales were lower than in HS silage (P < 0.05). Significant butyrate concentrations were detected only in DM25 silage. Round-bale silages were chopped before feeding and fed ad libitum. DM intake was 14.5% higher for DM50 and DM40 silages than for DM25 and HS silages (P < 0.001). Average daily gain and feed efficiency were higher for high-DM silages than for DM25 and HS silages (P < 0.01). Apparent digestibilities of NDF, cellulose and crude fiber of the silages were similar, but DM, organic matter, energy and hemicellulose digestibilities were higher for HS than for DM25 silage. Apparent digestibilities of ADF and CP were higher for high-DM silages than for DM25 and HS silages (P < 0.04). The results indicate that to obtain good-quality silage, the DM content of round bales should be 40–50%. The better performance obtained with high-DM silages was indicative of a more efficient utilization of metabolizable energy for gain. Key words: Round-bale silage, timothy, forage

scholarly journals ENSILING CHARACTERISTICS AND NUTRITIVE VALUE OF CORN COBS AS AFFECTED BY ADDITION OF DIFFERENT LEVELS OF UREA AND SOLUBLE CARBOHYDRATES

2017 ◽  
Vol 48 (Special) ◽  
Author(s):  
Saeed & Mohammed
Keyword(s):  
Volatile Fatty Acids ◽  
Nutritive Value ◽  
Nitrogen Concentration ◽  
Ammonia Nitrogen ◽  
Neutral Detergent Fiber ◽  
Soluble Carbohydrates ◽  
Corn Cobs ◽  
Aerobic Stability ◽  
Different Levels

This study was carried out in vitro to investigate the effect of addition of different levels of dates honey (DH) as soluble carbohydrates (WSC) and urea on fermentation quality and nutritive value of corn cobs silages (CCS). CC was ensiled with 4 levels of DH, 4, 6, 8 or 10% and 3 levels of urea 0, 1.5 or 3%. CCS samples were packed in double layer nylon bags and kept anaerobically for 60 days. Results revealed that color of CCS samples were ranged between yellow and brown. Smell of diluted vinegar was detected in samples made with addition of DH only, whereas, those made with addition of low and high levels of urea were characterized with smell of diluted and concentrated ammonia respectively. Increasing DH level from 4 to 10% significantly (P˂0.05) decrease in neutral detergent fiber (NDF) and hemicellulose by 2.42 and 2.69% respectively, with significant (P˂0.01) increase in crude protein (CP) and ether extract (EE). Significant (P˂0.05) decrease in crude fiber (CF) and NDF with significant (P˂0.01) increase in CP and cellulose contents were noticed due to addition of urea. Results also showed a trend for pH to be reduced (P˂0.01) with increasing level of DH, but different responses to increased urea levels were shown, where values were 5.91, 6.17 and 6.95 at levels of 0, 1.5 and 3%. Increasing DH level (6 and 8%) significantly (P˂0.01) decrease silage ammonia nitrogen concentration as a percentage of total nitrogen (18.52 and 18.35) and increase concentration of total volatile fatty acids as a percentage of silage dry matter (DM) at higher levels (6.73 and 8.24%), however both concentrations were reversely responded to increase urea level. Lower DM loss and buffering capacity (BC) were recorded at the lower level of DH (41.86 and 43.60 meq NaOH/100 g DM), whereas the higher level was associated with better Fleig point (Fp). Regarding effect of levels of urea, lower (P˂0.01) DM loss and BC with higher (P˂0.01) Fp at the lower level were observed, the later was 63.16. Results of study also revealed that increasing level of DH decreased aerobic stability of CCS samples to 41 hours (h), but higher value of 43.5 was recorded at higher level of urea. Results also showed that in vitro DM digestibility of CCS was (P˂0.05) improved due to increasing levels of DH and urea, however, lower (P˂0.05) values were obtained with lower levels of these additives.

scholarly journals Dissolution analysis to study release of Lactobacillus plantarum from silage inoculant prills

2011 ◽  
Vol 64 ◽  
pp. 286-286
Author(s):  
J. Swaminathan ◽  
C.R. Bunt ◽  
B. Gilmore ◽  
D. Jones
Keyword(s):  
Lactic Acid ◽  
Lactobacillus Plantarum ◽  
In Vitro Release ◽  
Immediate Release ◽  
Water Soluble ◽  
Soluble Carbohydrates ◽  
Bacterial Survival ◽  
Release Formulation ◽  
Neutral Conditions

The quality of silage can be improved by addition of lactic acid bacteria (LAB) at the time of ensiling Rapid fermentation by fermentative LAB reduces water soluble carbohydrates to lactic acid The consequent rapid decrease in pH improves silage preservation and inhibits the growth of aerobic spoilage microorganisms For successful silage making it is essential that inoculants are delivered in a viable state and are released during silage making LAB can be formulated as prills for extended bacterial survival but the release characteristics from the prill will determine product utility In these experiments release characteristics of the LAB Lactobacillus plantarum contained in three types of prills (immediate release formulation (IRF) or sustained release formulations (SRF1 and SRF2)) were examined using a DIS 8000 Dissolution Apparatus to study in vitro release in two pH conditions At neutral pH IRF released >80 of live bacterial within 30 min while SRF1 and SRF2 released approximately 55 and 25 respectively Under acidic conditions (pH 4) release profiles were similar to those produced under neutral conditions from all formulations This study demonstrates the utility of dissolution analysis in selection of bacterial formulations

Effect of Lactobacillus plantarum and Lactobacillus buchneri addition on fermentation, bacterial community and aerobic stability in lucerne silage

2019 ◽  
Vol 59 (8) ◽  
pp. 1528
Author(s):  
Huazhe Si ◽  
Hanlu Liu ◽  
Zhipeng Li ◽  
Weixiao Nan ◽  
Chunai Jin ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Microbial Community ◽  
Lactic Acid Bacteria ◽  
Lactobacillus Plantarum ◽  
Water Soluble ◽  
Metabolic Phenotype ◽  
Residual Water ◽  
Lactobacillus Buchneri ◽  
Silage Fermentation ◽  
Aerobic Stability

Changes in the microbial community are closely related to the fermentation of silage. However, how host genetic variation shapes the community structure of the silage microbiota and its metabolic phenotype is poorly understood. The objective of present study was to evaluate the effects of the application of the homo-fermentative Lactobacillus plantarum and hetero-fermentative Lactobacillus buchneri strains to lucerne silage on the fermentation characteristics, aerobic stability, and microbial community and their correlations. The three silages treated with L. plantarum or L. buchneri were well preserved and had significantly lower pH values, butyric acid, propionic acid, and ammonia-N concentrations, and significantly higher residual water-soluble carbohydrate, dry matter and lactic acid contents than the controls. The treated groups had more lactic acid bacteria and lower quantities of other bacteria in their microbial communities. Inoculation of lactic acid bacteria influenced the abundances of other bacteria and controlled the silage fermentation characteristics. L. buchneri inhibited the abundance of Enterobacter_ludwigii to increase the crude protein content, L. plantarum improve the neutral detergent fibre content by affecting the abundance of Arthrobacter_sp._Ens13. In conclusion, the application of L. plantarum and L. buchneri improved the quality of lucerne silage fermentation, and L. buchneri resulted in greater improvements after aerobic exposure.

Sign in / Sign up

Export Citation Format

Share Document