scholarly journals Dissolution analysis to study release of Lactobacillus plantarum from silage inoculant prills

2011 ◽  
Vol 64 ◽  
pp. 286-286
Author(s):  
J. Swaminathan ◽  
C.R. Bunt ◽  
B. Gilmore ◽  
D. Jones
Keyword(s):  
Lactic Acid ◽  
Lactobacillus Plantarum ◽  
In Vitro Release ◽  
Immediate Release ◽  
Water Soluble ◽  
Soluble Carbohydrates ◽  
Bacterial Survival ◽  
Release Formulation ◽  
Neutral Conditions

The quality of silage can be improved by addition of lactic acid bacteria (LAB) at the time of ensiling Rapid fermentation by fermentative LAB reduces water soluble carbohydrates to lactic acid The consequent rapid decrease in pH improves silage preservation and inhibits the growth of aerobic spoilage microorganisms For successful silage making it is essential that inoculants are delivered in a viable state and are released during silage making LAB can be formulated as prills for extended bacterial survival but the release characteristics from the prill will determine product utility In these experiments release characteristics of the LAB Lactobacillus plantarum contained in three types of prills (immediate release formulation (IRF) or sustained release formulations (SRF1 and SRF2)) were examined using a DIS 8000 Dissolution Apparatus to study in vitro release in two pH conditions At neutral pH IRF released >80 of live bacterial within 30 min while SRF1 and SRF2 released approximately 55 and 25 respectively Under acidic conditions (pH 4) release profiles were similar to those produced under neutral conditions from all formulations This study demonstrates the utility of dissolution analysis in selection of bacterial formulations

scholarly journals Effects of additives on the fermentation quality, in vitro digestibility and aerobic stability of mulberry (Morus alba L.) leaves silage

2020 ◽  
Vol 33 (8) ◽  
pp. 1292-1300
Author(s):  
Zhihao Dong ◽  
Siran Wang ◽  
Jie Zhao ◽  
Junfeng Li ◽  
Tao Shao
Keyword(s):  
Lactic Acid ◽  
Lactobacillus Plantarum ◽  
Water Soluble ◽  
Soluble Carbohydrates ◽  
In Vitro Digestibility ◽  
Fresh Matter ◽  
Mulberry Leaves ◽  
Aerobic Stability ◽  
Fermentation Quality

Objective: To explore feed resources capable of replacing regular poor-quality fodder, this study was conducted to evaluate the effects of additives on the fermentation quality, <i>in vitro</i> digestibility and aerobic stability of mulberry leaves silage.Methods: The mulberry leaves were ensiled either untreated (control) or treated with 1×10<sup>6</sup> cfu/g fresh matter <i>Lactobacillus plantarum</i> (L), 1% glucose (G), 3% molasses (M), a combination of 1% glucose and <i>Lactobacillus plantarum</i> (L+G), and a combination of 3% molasses and <i>Lactobacillus plantarum</i> (L+M). The fermentation quality and chemical composition were analyzed after 7, 14, 30, and 60 d, respectively. The 60-d silages were subjected to an aerobic stability test and fermented with buffered rumen fluid to measure the digestibility.Results: Inoculating lactic acid bacteria (LAB) resulted in more rapid increase in lactic acid concentrations and decline in pH of mulberry leaves silage as compared control. Higher acetic acid and lower ethanol and ammonia nitrogen concentrations (p<0.05) were observed in the LAB-inoculated silages as opposed to control during ensiling. The LAB-inoculated silages contained lower water-soluble carbohydrates compared with control during the first 14 d of ensiling, and lower neutral detergent fibre (p<0.05) concentrations as compared with non-LAB inoculated silages. Adding molasses alone increased (p<0.05) the digestibility of dry matter (DM). The aerobic stability of mulberry leaves silage was increased by LAB inoculation, whereas decreased by adding glucose or molasses.Conclusion: The LAB inoculation improved fermentation quality and aerobic stability of mulberry leaves silage, while adding glucose or molasses failed to affect the fermentation and impaired the aerobic stability. Inoculating LAB alone is recommended for mulberry leaves especially when ensiled at a relatively high DM.

Intake, digestibility and aerobic stability of barley silage inoculated with mixtures of Lactobacillus plantarum and Enterococcus faecium

1995 ◽  
Vol 75 (3) ◽  
pp. 425-432 ◽  
Author(s):  
T. A. McAllister ◽  
L. B. Selinger ◽  
L. R. McMahon ◽  
H. D. Bae ◽  
T. J. Lysyk ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactobacillus Plantarum ◽  
Enterococcus Faecium ◽  
Volatile Fatty Acids ◽  
Average Daily Gain ◽  
Water Soluble ◽  
Neutral Detergent Fiber ◽  
Soluble Carbohydrates ◽  
Aerobic Stability ◽  
Barley Silage

The effect of ensiling barley treated with two bacterial inoculants containing mixtures of Lactobacillus plantarum and Enterococcus faecium (1.0 × 105 cfu g−1 as fed silage) on the nutritional value and aerobic stability of barley silage was examined. Inoculants differed in the strains they contained and were originally selected by Pioneer Hi-Bred International for use with corn or alfalfa silage, SILA-BAC® (1174), or with grass silage (X2637). Concentrations of water-soluble carbohydrates were higher (P < 0.05) in inoculated than in control silages. Although inoculants appeared to increase the numbers of lactic acid producing bacteria (LAB) at ensiling, post-ensiling numbers (cfu g−1) of yeasts and molds were lower (P < 0.05) in inoculated than in control silages. Lactic acid concentrations and pH were similar among the silages and variations m the growth of yeast and mold populations could not be explained by differences in the production of volatile fatty acids (VFA) among silages. Inoculation of barley silage with either inoculant increased (P < 0.01) the average daily gain of lambs. A digestibility experiment with 12 growing ram lambs showed that inoculants did not alter (P > 0.05) DM intake, feed efficiency or the digestion of DM, organic matter, acid detergent fiber (ADF) and neutral detergent fiber (NDF). Nitrogen intake and retention were greater (P < 0.05) in lambs fed silage inoculated with 1174 as compared with control silage. Yeast populations were increased (P < 0.05) in control and 1174 after 2 d of exposure to air but it required 13 d for a similar yeast population to be established in X2637 silage. Increases in the mold populations within the silages were noted after 2, 5 and 13 d of exposure to air for control, 1174 and X2637, respectively. The temperature of control silage increased (P < 0.05) 2 d after exposure to air, whereas increases in temperature were delayed for 4 d in 1174 and 8 d in X2637. Temperatures rose as high as 30 °C in control silage, but did not exceed 24 °C in inoculated silages during the 13 d period. Key words: Barley silage, inoculant, digestion, aerobic stability, sheep, gain

scholarly journals DRUG RELEASE CONTROL AND ENHANCEMENT USING CARRIERS WITH DIFFERENT CONCENTRATIONS OF CAPMUL® MCM C8

2021 ◽  
pp. 249-252
Author(s):  
MOHAMMAD F. BAYAN
Keyword(s):  
Drug Release ◽  
Oral Delivery ◽  
Drug Carrier ◽  
In Vitro Release ◽  
Water Soluble ◽  
Swelling Behavior ◽  
Release Formulation ◽  
Poorly Soluble ◽  
Model Drug

Objective: The main aim of this study was to design a drug carrier capable to control and enhance the release of poorly water soluble drugs. Methods: Three polymeric formulations, based on poly (2-hydroxyethyl methacrylate) and loaded with different Capmul® MCM C8 concentrations (0, 10 and 20 % w/w), were prepared. Felodipine, which is a poorly soluble substance, was selected as a model drug. The effect of Capmul® MCM C8 on swelling behavior and in vitro release profile of the prepared polymer was investigated in PBS. Results: The swelling profiles of allformulationswere statistically similar, which indicated the non-significant effect of added Capmul® MCM C8 on polymer's swelling behavior. All formulations showed a delayed drug release. Formulation-F3, which is loaded with 20% w/wCapmul® MCM C8 displayed a significant higher release compared to the other formulations. Conclusion: Capmul® MCM products, which are widely used in food industries, can be used to improve the oral delivery of poorly soluble substances. The optimized formulation exhibited the ability to control and enhance the release of the model drug.

Controlled Release of Metformin Hydrochloride I. In vitro Release from Physical Mixture Containing Xanthan Gum as Hydrophilic Rate Retarding Polymer

1970 ◽  
Vol 4 (1) ◽  
Author(s):  
Mashkura Ashrafi ◽  
Jakir Ahmed Chowdhury ◽  
Md Selim Reza
Keyword(s):  
Controlled Release ◽  
Xanthan Gum ◽  
In Vitro Release ◽  
Correlation Coefficients ◽  
High Ratio ◽  
Water Soluble ◽  
Metformin Hydrochloride ◽  
Model Drug ◽  
Very High

Capsules of different formulations were prepared by using a hydrophilic polymer, xanthan gum and a filler Ludipress. Metformin hydrochloride, which is an anti-diabetic agent, was used as a model drug here with the aim to formulate sustained release capsules. In the first 6 formulations, metformin hydrochloride and xanthan gum were used in different ratio. Later, Ludipress was added to the formulations in a percentage of 8% to 41%. The total procedure was carried out by physical mixing of the ingredients and filling in capsule shells of size ‘1’. As metformin hydrochloride is a highly water soluble drug, the dissolution test was done in 250 ml distilled water in a thermal shaker (Memmert) with a shaking speed of 50 rpm at 370C &plusmn 0.50C for 6 hours. After the dissolution, the data were treated with different kinetic models. The results found from the graphs and data show that the formulations follow the Higuchian release pattern as they showed correlation coefficients greater than 0.99 and the sustaining effect of the formulations was very high when the xanthan gum was used in a very high ratio with the drug. It was also investigated that the Ludipress extended the sustaining effect of the formulation to some extent. But after a certain period, Ludipress did not show any significant effect as the pores made by the xanthan gum network were already blocked. It is found here that when the metformin hydrochloride and the xanthan gum ratio was 1:1, showed a high percentage of drug release, i.e. 91.80% of drug was released after 6 hours. But With a xanthan gum and metformin hydrochloride ratio of 6:1, a very slow release of the drug was obtained. Only 66.68% of the drug was released after 6 hours. The percent loading in this case was 14%. Again, when Ludipress was used in high ratio, it was found to retard the release rate more prominently. Key words: Metformin Hydrochloride, Xanthan Gum, Controlled release capsule Dhaka Univ. J. Pharm. Sci. Vol.4(1) 2005 The full text is of this article is available at the Dhaka Univ. J. Pharm. Sci. website

Fenofibrate Solid Dispersion for Improving Oral Bioavailability: Preparation, and Characterization and in vivo Evaluation

2020 ◽  
Vol 13 (5) ◽  
pp. 5102-5109
Author(s):  
Venu Madhav K ◽  
Somnath De ◽  
Chandra Shekar Bonagiri ◽  
Sridhar Babu Gummadi
Keyword(s):  
Oral Bioavailability ◽  
Oral Delivery ◽  
Solid Dispersions ◽  
In Vitro Release ◽  
Aqueous Solubility ◽  
Water Soluble ◽  
Scanning Calorimetry ◽  
In Vivo Evaluation

Fenofibrate (FN) is used in the treatment of hypercholesterolemia. It shows poor dissolution and poor oral bioavailability after oral administration due to high liphophilicity and low aqueous solubility. Hence, solid dispersions (SDs) of FN (FN-SDs) were develop that might enhance the dissolution and subsequently oral bioavailability. FN-SDs were prepared by solvent casting method using different carriers (PEG 4000, PEG 6000, β cyclodextrin and HP β cyclodextrin) in different proportions (0.25%, 0.5%, 0.75% and 1% w/v). FN-SDs were evaluated solubility, assay and in vitro release studies for the optimization of SD formulation. Differential scanning calorimetry (DSC), powder X-ray diffraction (PXRD) and scanning electron microscopy (SEM) analysis was performed for crystalline and morphology analysis, respectively. Further, optimized FN-SD formulation evaluated for pharmacokinetic performance in Wistar rats, in vivo in comparison with FN suspension.  From the results, FN-SD3 and FN-SD6 have showed 102.9 ±1.3% and 105.5±3.1% drug release, respectively in 2 h. DSC and PXRD studies revealed that conversion of crystalline to amorphous nature of FN from FT-SD formulation. SEM studies revealed the change in the orientation of FN when incorporated in SDs. The oral bioavailability FN-SD3 and FN-SD6 formulations exhibited 2.5-folds and 3.1-folds improvement when compared to FN suspension as control. Overall, SD of FN could be considered as an alternative dosage form for the enhancement of oral delivery of poorly water-soluble FN.

Formulation and Evaluation of Immediate Release Bilayer Tablets of Telmisartan and Hydrochlorothiazide

2011 ◽  
Vol 4 (3) ◽  
pp. 1477-1483
Author(s):  
Natarajan R ◽  
N Patel ◽  
Rajendran N N ◽  
M Rangapriya
Keyword(s):  
Antihypertensive Drugs ◽  
In Vitro Release ◽  
Immediate Release ◽  
Wet Granulation ◽  
Physical Parameters ◽  
Dissolution Profile ◽  
Formulation Development ◽  
Sodium Starch Glycolate ◽  
Bilayer Tablets

The main goal of this study was to develop a stable formulation of antihypertensive drugs telmisartan and hydrochlorothiazide as an immediate-release bilayer tablet and to evaluate the dissolution profile in comparison with a reference product. The formulation development work was initiated with wet granulation. Telmisartan was converted to its sodium salt by dissolving in aqueous solution of sodium hydroxide to improve solubility and drug release. Lactose monohydrate and microcrystalline cellulose were used as diluents. Starch paste is prepared in purified water and was used as the binder. Sodium starch glycolate is added as a disintegrating agent. Magnesium stearate was used as the lubricant. The prepared granules were compressed into a double-layer compression machine. The tablets thus formulated with higher proportion of sodium starch glycolate showed satisfactory physical parameters, and it was found to be stable and in vitro release studies are showed that formulation (F-T5H5) was 101.11% and 99.89% respectively. The formulation T5H5 is further selected and compared with the release profile of the innovator product, and was found to be similar (f2 factor) to that of the marketed product. The results suggest the feasibility of developing bilayer tablets consisting of telmisartan and hydrochlorothiazide for the convenience of patients with hypertension.  

Solubility Enhancement of Poorly Water-Soluble Antifungal Drug Acyclovir by Nanocrystal Formulation Approach

2018 ◽  
Vol 11 (2) ◽  
pp. 4036-4042
Author(s):  
Prakash Goudanavar ◽  
Ankit Acharya ◽  
Vinay C.H
Keyword(s):  
Chemical Interaction ◽  
Research Work ◽  
Antiviral Drug ◽  
In Vitro Release ◽  
Oral Route ◽  
Water Soluble ◽  
Precipitation Method ◽  
Dsc Studies ◽  
Ft Ir

Administration of an antiviral drug, acyclovir via the oral route leads to low and variable bioavailability (15-30%). Therefore, this research work was aimed to enhance bioavailability of acyclovir by nanocrystallization technique. The drug nanocrystals were prepared by anti-solvent precipitation method in which different stabilizers were used. The formed nanocrystals are subjected to biopharmaceutical characterization including solubility, particle size and in-vitro release. SEM studies showed nano-crystals were crystalline nature with sharp peaks. The formulated drug nanocrystals were found to be in the range of 600-900nm and formulations NC7 and NC8 showed marked improvement in dissolution velocity when compared to pure drug, thus providing greater bioavailability. FT-IR and DSC studies revealed the absence of any chemical interaction between drug and polymers used. 

scholarly journals Dissolution Testing of Nicotine Release from OTDN Pouches: Product Characterization and Product-to-Product Comparison

Separations ◽  
2021 ◽  
Vol 8 (1) ◽  
pp. 7
Author(s):  
Fadi Aldeek ◽  
Nicholas McCutcheon ◽  
Cameron Smith ◽  
John H. Miller ◽  
Timothy L. Danielson
Keyword(s):  
In Vitro Release ◽  
Product Category ◽  
Immediate Release ◽  
Tobacco Product ◽  
Oral Dosage ◽  
Dissolution Testing ◽  
Release Rates ◽  
Usp 4 ◽  
Release Profiles

In recent years, oral tobacco-derived nicotine (OTDN) pouches have emerged as a new oral tobacco product category. They are available in a variety of flavors and do not contain cut or ground tobacco leaf. The on!® nicotine pouches fall within this category of OTDN products and are currently marketed in seven (7) flavors with five (5) different nicotine levels. Evaluation of the nicotine release from these products is valuable for product assessment and product-to-product comparisons. In this work, we characterized the in vitro release profiles of nicotine from the 35 varieties of on!® nicotine pouches using a fit-for-purpose dissolution method, employing the U.S. Pharmacopeia flow-through cell dissolution apparatus 4 (USP-4). The nicotine release profiles were compared using the FDA’s Guidance for Industry: Dissolution Testing of Immediate Release Solid Oral Dosage Forms. The cumulative release profiles of nicotine show a dose dependent response for all nicotine levels. The on!® nicotine pouches exhibit equivalent percent nicotine release rates for each flavor variant across all nicotine levels. Furthermore, the nicotine release profiles from on!® nicotine pouches were compared to a variety of other commercially available OTDN pouches and traditional pouched smokeless tobacco products. The percent nicotine release rates were found to be dependent on the product characteristics, showing similarities and differences in the nicotine release profiles between the on!® nicotine pouches and other compared products.

scholarly journals Probiotic Potential of a Novel Vitamin B2-Overproducing Lactobacillus plantarum Strain, HY7715, Isolated from Kimchi

2021 ◽  
Vol 11 (13) ◽  
pp. 5765
Author(s):  
Joo-Yun Kim ◽  
Eun-Jung Choi ◽  
Jae-Ho Lee ◽  
Myeong-Seok Yoo ◽  
Keon Heo ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Lactobacillus Plantarum ◽  
Probiotic Strain ◽  
Optimal Growth ◽  
Control Group ◽  
High Survival ◽  
Vitamin B2 ◽  
Riboflavin Deficiency

Vitamin B2, also known as riboflavin, is essential for maintaining human health. The purpose of this study was to isolate novel lactic acid bacteria that overproduce vitamin B2 and to validate their potential as probiotics. In this study, Lactobacillus plantarum HY7715 (HY7715) was selected among lactic acid bacteria isolated from Kimchi. HY7715 showed a very high riboflavin-producing ability compared to the control strain due to the high expression of ribA, ribB, ribC, ribH, and ribG genes. HY7715 produced 34.5 ± 2.41 mg/L of riboflavin for 24 h without consuming riboflavin in the medium under optimal growth conditions. It was able to produce riboflavin in an in vitro model of the intestinal environment. In addition, when riboflavin deficiency was induced in mice through nutritional restriction, higher levels of riboflavin were detected in plasma and urine in the HY7715 administration group than in the control group. HY7715 showed high survival rate in simulated gastrointestinal conditions and had antibiotic resistance below the cutoff MIC value suggested by the European Food Safety Authority; moreover, it did not cause hemolysis. In conclusion, HY7715 could be considered a beneficial probiotic strain for human and animal applications, suggesting that it could be a new alternative to address riboflavin deficiency.

The assessment of herbage legume varieties. II. In vitro digestibility, water soluble carbohydrate, crude protein and mineral content of primary growth of clover and lucerne

1966 ◽  
Vol 66 (3) ◽  
pp. 351-357 ◽  
Author(s):  
W. Ellis Davies ◽  
G. ap Griffith ◽  
A. Ellington
Keyword(s):  
White Clover ◽  
Crude Protein ◽  
Dry Matter ◽  
Red Clover ◽  
Water Soluble ◽  
Soluble Carbohydrates ◽  
Soluble Carbohydrate ◽  
In Vitro Digestibility ◽  
Primary Growth

The primary growth of eight varieties of three species–white clover (3), red clover (4) and lucerne (1)–was sampled at fortnightly intervals and the percentage dry matter, in vitro digestibility, crude protein, water soluble carbohydrates, P, Ca, K, Na and Mg were determined.Differences between species were nearly always significant and the general order of merit was white clover, red clover and lucerne. The exceptions were for dry-matter percentage where this order was reversed, and red clover had the lowest Na and highest Mg content.

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