METHODS OF BREEDING FOR OIL QUALITY IN RAPE

1963 ◽  
Vol 43 (3) ◽  
pp. 271-275 ◽  
Author(s):  
R. K. Downey ◽  
B. L. Harvey

Reciprocal crosses were made between plants of Brassica napus with seed oil containing 40 and 0 per cent erucic acid. Gas liquid chromatographic analysis snowed an erucic acid content of 22 to 24 per cent in the crossed seeds. These results indicate that dominant gene action is absent and that fatty acid composition of the oil is controlled by the developing embryo. Thus the seed on an F1 plant constitutes an F2 population.Methods were developed to determine the fatty acid composition of oil from single seeds and from one cotyledon of an embryo. The analysis of a single cotyledon allows the genotype to be determined one generation earlier than if bulk samples are used, and the remainder of the seed may be grown into a normal plant. It is suggested that this half-seed technique may be used to determine the inheritance of other fatty acids in Brassica, and may also be applied to breeding other oil crops where the embryo, rather than the maternal sporophyte, controls the synthesis of fatty acids.

2021 ◽  
Vol 72 (3) ◽  
pp. e415 ◽  
Author(s):  
M. De Wit ◽  
V.K. Motsamai ◽  
A. Hugo

Cold-pressed seed oil from twelve commercially produced cactus pear cultivars was assessed for oil yield, fatty acid composition, physicochemical properties, quality and stability. Large differences in oil content, fatty acid composition and physicochemical properties (IV, PV, RI, tocopherols, ORAC, % FFA, OSI and induction time) were observed. Oil content ranged between 2.51% and 5.96% (Meyers and American Giant). The important fatty acids detected were C16:0, C18:0, C18:1c9 and C18:2c9,12, with C18:2c9,12, the dominating fatty acid, ranging from 58.56-65.73%, followed by C18:1c9, ranging between 13.18-16.07%, C16:0, which ranged between 10.97 - 15.07% and C18:0, which ranged between 2.62-3.18%. Other fatty acids such as C14:0, C16:1c9, C17:0, C17:1c10, C20:0, C18:3c9,12,15 and C20:3c8,11,14 were detected in small amounts. The quality parameters of the oils were strongly influenced by oil content, fatty acid composition and physicochemical properties. Oil content, PV, % FFA, RI, IV, tocopherols, ORAC and ρ-anisidine value were negatively correlated with OSI. C18:0; C18:1c9; C18:2c9,12; MUFA; PUFA; n-6 and PUFA/SFA were also negatively correlated with OSI. Among all the cultivars, American Giant was identified as the paramount cultivar with good quality traits (oil content and oxidative stability).


2014 ◽  
Vol 13 (1) ◽  
pp. 83-89 ◽  
Author(s):  
Gerald J. Seiler

Monitoring and protecting germplasm in genebanks using in situ collections while preserving its original genetic integrity is a priority of germplasm curation. Many germplasm accessions need to be regenerated due to their demand and/or seed condition. The regeneration of wild Helianthus (sunflower) species poses several challenges due to the diversity of 53 wild species. Fatty acid composition of sunflower oil is an important quality factor for the crop. Since oil quality is environmentally influenced, and evaluation of this trait is usually performed on oil from achenes from the original accessions of wild sunflower species, we conducted a study on 72 accessions of eight annual and four perennial taxa of wild sunflower species to compare the oil quality of the original accessions and those regenerated for genebank maintenance. The results showed that the fatty acid composition profiles of achenes from the original and regenerated accessions are not the same. It seems that selection for specific fatty acids in several species will require the analysis of both populations to identify germplasm accessions for use in breeding programmes. It should be borne in mind that accessions of wild species are open-pollinated segregating populations, so one would expect some variability in each succeeding generation. While there may be differences between the original and regenerated accessions, the interrelationships of fatty acids are generally similar in wild and cultivated sunflower species, so there should be no detrimental effects on oil quality when using the wild species for other traits. As more regenerated accessions become available, a more precise relationship between the original and regenerated accessions should emerge.


Author(s):  
Md. Delwar Hossain ◽  
Kamal Uddin Ahmed ◽  
Mst. Farhana Nazneen Chowdhury ◽  
Alak Barman ◽  
Arif Ahmed ◽  
...  

With a view to studying the qualitative features and the variations in fatty acid composition of 6 rapeseed (B. campestris and B. napus) and mustard (B. juncea) varieties, an experiment was conducted. Among these varieties, BARI Sarisha-14 presented the value of 168.4 which was recorded the highest. Both BARI Sarisha-11 and BARI Sarisha-14 was found with the highest iodine value of 39.44; and the highest amount of acid value was recorded from BARI Sarisha-11 (1.867). Gas-liquid chromatographic (GLC) method has been used to determine the composition of essential fatty acid in the seeds of Brassica spp. (L.). From the GLC analysis, it was found that erucic acid, oleic acid, linoleic acid and lenolenic acid were the prime fatty acids in all the varieties. Erucic acid was in the range of 41.11 – 51.28%, oleic acid was the highest both in BARI Sarisha-11 and BARI Sarisha- 13 contained (18.69%), while BARI Sarisha-9 contained the highest amount of the unsaturated linoleic (17.75%)  and linolenic (15.83%) acids. Moreover, palmitic acid, stearic acid and archidic acid were also present in small amount.


Agronomy ◽  
2020 ◽  
Vol 10 (8) ◽  
pp. 1186 ◽  
Author(s):  
Ibrahim A. A. Mohamed ◽  
Nesma Shalby ◽  
Ali M. A. El-Badri ◽  
Muhammad Hamzah Saleem ◽  
Mohammad Nauman Khan ◽  
...  

Salinity stress is a limiting factor for the growth and yield quality of rapeseed. The potentiality of melatonin (MT; 0, 25, 50, and 100 µM) application as a seed priming agent in mediating K+/Na+ homeostasis and preventing the salinity stress mediated oxidative damage and photosynthetic inhibition was studied in two rapeseed cultivars. We found that 50 µM MT treatment imparted a very prominent impact on growth, metabolism of antioxidants, photosynthesis, osmolytes, secondary metabolites, yield, and fatty acids composition. Days required for appearance of first flower and 50% flowering were decreased by MT application. Exogenous MT treatment effectively decreased the oxidative damage by significantly declining the generation of superoxide and hydrogen peroxide under saline and non-saline conditions, as reflected in lowered lipid peroxidation, heightened membrane stability, and up-regulation of antioxidant enzymes (catalase, superoxide dismutase, and ascorbate peroxidase). Furthermore, MT application enhanced the chlorophyll content, photosynthetic rate, relative water content, K+/Na+ homeostasis, soluble sugars, and proline content. Moreover, MT application obviously improved the oil quality of rapeseed cultivars by reducing glucosinolates, saturated fatty acids (palmitic and arachidic acids), and enhancing unsaturated fatty acids (linolenic and oleic acids except erucic acid were reduced). Yield related-traits such as silique traits, seed yield per plant, 1000 seeds weight, seed oil content, and yield biomass traits were enhanced by MT application. The anatomical analysis of leaf and stem showed that stomatal and xylem vessels traits are associated with sodium chloride tolerance, yield, and seed fatty acid composition. These results suggest the supportive role of MT on the quality and quantity of rapeseed oil yield.


1959 ◽  
Vol 39 (4) ◽  
pp. 437-442 ◽  
Author(s):  
B. M. Craig ◽  
L. R. Wetter

The content of C16, C18, C20, C22 fatty acids were measured by gas liquid phase chromatography and linoleic and linolenic acids by spectral analyses on the oil from seven varieties of rapeseed grown at seven stations in Western Canada. Significant differences were found between varieties for all oil properties except the content of C16 acids. The major variation occurred in C18, C22 and linoleic acids with lesser amounts in the C20 and linolenic acids. The varieties Golden, Argentine, Regina II and Swedish are classed as high, Gute and Arlo as intermediate, and Polish as low erucic acid oils.


2000 ◽  
Vol 28 (6) ◽  
pp. 581-582 ◽  
Author(s):  
N. Kaushik ◽  
A. Agnihotri

Rapeseed-mustard is one of the most economically important oilseed crops in India. Speciality oils having high amounts of a specific fatty acid are of immense importance for both nutritional and industrial purposes. Oil high in oleic acid has demand in commercial food-service applications due to a long shelf-life and cholesterol-reducing properties. Both linoleic and linolenic acids are essential fatty acids; however, less than 3% linolenic acid is preferred for oil stability. High erucic acid content is beneficial for the polymer industry, whereas low erucic acid is recommended for food purposes. Therefore, it is important to undertake systematic characterization of the available gene pool for its variable fatty acid profile to be utilized for specific purposes. In the present study the Indian rapeseed-mustard germplasm and some newly developed low-erucic-acid strains were analysed by GLC to study the fatty acid composition in these lines. The GLC analysis revealed that the rapeseed-mustard varieties being commonly grown in India are characterized by high erucic acid content (30–51%) in the oil with low levels of oleic acid (13–23%). However, from among the recently developed low-erucic-acid strains, several lines were identified with comparatively high oleic acid (60–70%), moderate to high linoleic acid (13–40%) and low linolenic acid (< 10%) contents. Work is in progress at TERI (New Delhi, India) to utilize these lines for development of strains with particular fatty acid compositions for specific purposes.


2020 ◽  
Vol 65 (1) ◽  
Author(s):  
Vera Gavrilova ◽  
Tatyana Shelenga ◽  
Elizaveta Porokhovinova ◽  
Aleksandra Dubovskaya ◽  
Nina Kon’kova ◽  
...  

This review is devoted to the description of chemical peculiarities of industrial oil crops cultivated (or prospective for cultivation) in Russia, which are stored in the VIR collection. Different crops have similar fatty acids biosynthesis pathways, but each species has its own individualities in the chemical composition of the oil and its genetic control. The diversity of oil crop chemical composition opens the possibility of its multipurpose utilization practically in all industrial segments. Sunflower, rapeseed, flax, mustard, camelina and safflower are cultivated in Russia as oil crops. Castor beans, perilla, lallemantia and noog are not cultivated on an industrial scale, but have original oil properties and are prospective for future cultivation. Hemp and poppy seeds contain oil valuable for food, but they are not widespread. Cotton and peanut oils are prospective for industrial purposes when early, already created varieties of these crops will be cultivated in Russia. Oil properties depend on the ratio of its basic fatty acids: saturated (stearic, palmitic) and unsaturated (oleic, linoleic, linolenic). As a rule, lauric, myristic and palmitoleic acids are determined in minor quantities. The oil of Brassicaceae crops also includes arachidic, eicosenoic, eicosadienoic, behenic, erucic and lignoceric acids. Fatty acids accumulation is influenced by growing conditions, though it has strict genetic control.


1990 ◽  
Vol 17 (1) ◽  
pp. 17-21 ◽  
Author(s):  
L. C. Mercer ◽  
J. C. Wynne ◽  
C. T. Young

Abstract The stability or shelf-life of peanut (Arachis hypogaea L.) oil is related to the fatty acid content of the oil, with the major factor being the ratio of oleic (C18:1) to linoleic (C18:2) acid (O/L ratio). To obtain information needed for development of cultivars with improved oil quality, eight parents representing a range in oleic and linoleic content were crossed in diallel. Individual F1 seeds (F1 embryos) from the greenhouse and F2 bulk seed from the 56 crosses grown in the field were analyzed to determine levels of the eight major fatty acids. General combining ability (GCA) was consistently more important than specific combining ability (SCA) in both generations, suggesting that additive effects are important in the inheritance of fatty acid composition. Maternal effects were significant in the F1 but dissipated in the F2; thus the differences in the environment provided by the maternal parent was more critical to oil composition than heritable extranuclear factors. Reciprocal effects were significant in both generations suggesting an interaction between nuclear and extranuclear factors. Correlations between GCA effects and self means for O/L ratio were nonsignificant. Since no significant correlations were found between percent oil and any of the fatty acids or related variables, selection for improved fatty acid composition should not affect the oil content of seed. Of the lines studied, NC 7, NC-Fla 14, and 73–30 should be used as parents in a breeding program for oil quality.


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