A comparison of methods for the prediction of Cantonese noodle colour

1992 ◽  
Vol 72 (4) ◽  
pp. 1021-1029 ◽  
Author(s):  
J. E. Kruger ◽  
R. R. Matsuo ◽  
K. Preston
Keyword(s):  
Sodium Hydroxide ◽  
Polyphenol Oxidase ◽  
Quality Parameter ◽  
Laboratory Method ◽  
Alternative Methods ◽  
Comparison Of Methods ◽  
White Wheat ◽  
Flour Colour ◽  
The Common ◽  
Common Laboratory

The common laboratory method for preparing Cantonese raw noodle dough for colour evaluation requires 200–300 g flour, a Hobart mixer to mix the dough and an Ohtake laboratory noodle machine. Alternative methods requiring less flour or less costly apparatus were developed and compared. Although the brightness (L*) and yellowness (b*) were influenced by the method of noodle-dough preparation, variations between methods were consistent. Different formulations of Kansui had similar effects on noodle brightness and yellowness, with the exception of sodium hydroxide, which produced substantially increased yellowness. The rate of decrease in brightness with time was useful as a quality parameter in estimation of noodle colour and may be indicative, in some cases, of the presence of polyphenol oxidase enzymes. The ranges in brightness and yellowness of Cantonese noodle doughs are very similar for Canadian wheats, except for soft white wheat cultivars.Key words: Noodle, wheat, flour, colour, polyphenol oxidase

Partial Purification of Polyphenol Oxidase (PPO) from Mango Seed Kernel (MSK) using Alcohol/Salt-based Aqueous Two-Phase System (ATPS)

2021 ◽  
Vol 10 ◽  
Author(s):  
John Leanard M. Lasquite ◽  
Brendaline C. Enopia ◽  
Roger Francis E. Senangote ◽  
Danilo C. Sabarre Jr. ◽  
Camila Flor Yagonia-Lobarbio
Keyword(s):  
Polyphenol Oxidase ◽  
Alternative Methods ◽  
Phase System ◽  
Seed Kernel ◽  
Mass Ratio ◽  
Two Phase ◽  
Aqueous Two Phase System ◽  
Activity Recovery ◽  
Mango Seed Kernel ◽  
Mango Seed

Background: Polyphenol oxidase (PPO) has applications in pharmaceutical, environmental, and food industries, however, conventional techniques for its purification are considered costly and laborious. Alternative methods of purification are needed. Methods: This study explored the use of an alcohol/salt-based aqueous two-phase system (ATPS) to partially purify PPO from mango seed kernel (MSK). Specifically, this study determined the effects of 1-propanol/potassium phosphate buffer mass ratio on the partition parameters such as partition coefficients, activity recovery, and purification fold. Results: The enzyme partition coefficient (K E ) was greater than 1, which signified that more PPO was partitioned in the top phase due to its hydrophobic nature. Specifically, the 3.22 1-propanol/PPB mass ratio had the highest K E of 1.91 ± 0.16 and 1.46 ± 0.16 for runs 1 and 2, respectively. Moreover, the same mass ratio led to the highest activity recovery in the top phase reaching up to 73.99 ± 1.56% for run 1 and 64.27 ± 2.48% for run 2. In terms of purification fold, runs 1 and 2 had PF values of 0.55 ± 0.05 and 0.70 ± 0.01, respectively. Since the values obtained were low due to the large number of contaminants extracted together with the target enzyme in the top phase, the use of a back-extraction step recovered the PPO in the bottom phase and resulted in an activity recovery of 82.94 ± 1.05% and a purification fold of 7.01 ± 0.95. Conclusion: Results have shown that varying the mass ratio had significant effects on the partition parameters.

scholarly journals The life history of Hepatozoon leptodactyli (Lesage, 1908) Pessoa, 1970: a parasite of the common laboratory animal: the frog of the genus Leptodactylus

1973 ◽  
Vol 71 (1-2) ◽  
pp. 1-8 ◽  
Author(s):  
Sylvio Celso Goncalves da Costa ◽  
Samuel B. Pessoa ◽  
Neize de Moura Pereira ◽  
Tania Colombo
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Life History ◽  
Laboratory Animal ◽  
Peripheral Circulation ◽  
Experimental Conditions ◽  
History Of ◽  
The Central Nervous System ◽  
The Common ◽  
Common Laboratory

The main object of the present paper is to furnish a brief account to the knowledgement of Protozoa parasitic in common Brazilian frog of the genus Leptodactylus for general students in Zoology and for investigators that use this frog as a laboratory animal. Hepatozoon leptodactyli (Haemogregarina leptodactyli) was found in two species of frogs - Leptodactylus ocellatus and L. pentadactylus - in which develop schizogony whereas sporogony occurs in the leech Haementeria lutzi as was obtainded in experimental conditions. Intracellular forms have been found in peripheral circulation, chiefly in erythrocytes, but we have found them in leukocytes too. Tissue stages were found in frog, liver, lungs, spleen, gut, brain and heart. The occurence of hemogregarine in the Central Nervous System was recorded by Costa & al,(13) and Ball (2). Some cytochemical methods were employed in attempt to differentiate gametocytes from trophozoites in the peripheral blood and to characterize the cystic membrane as well. The speorogonic cycle was developed in only one specie of leech. A brief description of the parasite is given.

scholarly journals Experimental evaluation of organic substrates for germination testing of soybean seed

2020 ◽  
Vol 26 (2) ◽  
pp. 1-6
Author(s):  
Marija Milivojević ◽  
Dragana Branković-Radojčić ◽  
Radmila Vukadinović ◽  
Jasna Kojić ◽  
Zoran Dumanović ◽  
...  
Keyword(s):  
Soybean Seed ◽  
Laboratory Method ◽  
Alternative Methods ◽  
Routine Practice ◽  
Organic Substrates ◽  
Growing Media ◽  
Positive Effects ◽  
Temperature Regimes ◽  
High Germination ◽  
Crop Density

High germination of seeds and seedling establishment are critical for soybean production since it determines crop density and eventually affects the yield. Accurate evaluation of the germination potential of sowing material is valuable, as well as initial quality assessment of newly harvested seed. ISTA Rules prescribe alternative methods for soybean seed germination: sand, between paper, top of paper covered with sand and since 2020 organic growing media. In the Seed Testing Laboratory at the Maize Research Institute soybean germination is conducted in the sand at 20<=>30°C. The objective of this study was to evaluate several available organic growing media and compare results obtained with the standard laboratory method. Five soybean seed lots of different quality were tested in four germination growing media (3 organic and sand), at two temperature regimes (25 °C and 20<=>30°C). Statistical analyses showed that all types of organic growing media were of the same quality. Slightly higher germination in organic growing media was obtained in the experiment at 20<=>30°C compared to sand, but those differences were not statistically significant. When testing was done in germinator with 25°C positive effects of organic growing media were identified and this impact was significant, especially for low-quality seed lots. The experimental outcome was to continue with routine practice and use organic growing media for retesting of seed lots with lower germination as an alternative substrate.

Acute Liver Failure

2018 ◽  
Vol 39 (05) ◽  
pp. 513-522 ◽  
Author(s):  
Priyanka Rajaram ◽  
Ram Subramanian
Keyword(s):  
Liver Failure ◽  
Acute Liver Failure ◽  
Multiorgan Failure ◽  
Drug Induced ◽  
Detailed Review ◽  
Drug Induced Liver Injury ◽  
The Common ◽  
Organ Dysfunctions ◽  
Common Laboratory

AbstractAcute liver failure (ALF) is a condition that can rapidly progress to multiorgan failure. This article focuses on the diagnosis and management of ALF. We provide a detailed review of the common etiologies of ALF, including acetaminophen overdose, viral hepatitis, drug-induced liver injury, Wilson's disease, and autoimmune hepatitis. The article then addresses how to recognize ALF and reviews the role of common laboratory and imaging tests in establishing this diagnosis. The remainder of the article details the management of hepatic and extrahepatic organ dysfunctions in ALF. The article concludes with a discussion regarding the prognostication of patients with ALF and the criteria for considering liver transplantation.

Wet-Milling of Flours from Red, White and Low-Polyphenol Oxidase White Wheats

2005 ◽  
Vol 11 (4) ◽  
pp. 243-249 ◽  
Author(s):  
A. Sayaslan ◽  
P. A. Seib ◽  
O. K. Chung
Keyword(s):  
Wheat Flour ◽  
Polyphenol Oxidase ◽  
Water Soluble ◽  
High Yield ◽  
Wet Milling ◽  
White Wheat ◽  
Freeze Dried ◽  
Flour Yield ◽  
Milling Properties ◽  
Wheat Flours

Straight-grade and high-yield flours milled from red, white and low-polyphenol oxidase (PPO) activity white wheats were wet-milled to give gluten, starch, tailings and water-soluble fractions. Wet gluten fractions were either oven-dried or freeze-dried and ground to obtain dry gluten. White wheats yielded slightly more flour with higher lightness ( L*) than the red wheat. The wet-milling properties of all flours were comparable. The wet and oven-dried gluten fractions isolated from the low-PPO flours were the lightest, followed by the gluten fractions from the white and red wheat flours. The L* of the oven-dried gluten fractions from the low-PPO flours were ~ 1-3% higher than those from the white and red wheat flours. As the flour yield increased, the L* of the dry gluten fractions from all flours decreased likewise. However, the high-yield low-PPO white wheat flour gave the dry gluten with almost equal L* to the gluten isolated from the straight-grade red wheat flour, indicating the potential of the low-PPO white wheat flour in manufacturing brighter gluten.

Paper ionophoresis of carbohydrates. I. Procedures and results for four electrolytes

1959 ◽  
Vol 12 (1) ◽  
pp. 65 ◽  
Author(s):  
JL Frahn ◽  
JA Mills
Keyword(s):  
Sodium Hydroxide ◽  
Lead Acetate ◽  
Sodium Arsenite ◽  
Reducing Sugars ◽  
Sugar Alcohols ◽  
Polyhydroxy Compounds ◽  
Hexose Sugars ◽  
The Common ◽  
Derivatives Of

Details are given of procedures for effecting paper ionophoresis of polyhydroxy compounds in the electrolytes borax, sodium arsenite, basic lead acetate, and sodium hydroxide. and for detecting the compounds after ionophoresis. Rates of migration are reported for 96 compounds, including all pentose and hexose sugars, the common disaccharides, all sugar alcohols up to the heptitols, the cyclitols, a number of glycols, and several glycosides and other derivatives of carbohydrates. Some new or improved reagents have been developed for locating carbohydrates on paper strips under various conditions. Sodium arsenite and basic lead acetate are the most effective electrolytes for separating reducing sugars, basic lead acetate is the best for separating sugar alcohols, and borax is the best for simple glycols. Some success has been achieved in correlating the configurations of stereoisomers with their mobilities in paper ionophoresis.

scholarly journals Assays for the rapid and specific identification of North AmericanYersinia pestisand the common laboratory strain CO92

BioTechniques ◽  
2008 ◽  
Vol 44 (2) ◽  
pp. 201-207 ◽  
Author(s):  
Amy J. Vogler ◽  
Elizabeth M. Driebe ◽  
Judy Lee ◽  
Raymond K. Auerbach ◽  
Christopher J. Allender ◽  
...  
Keyword(s):  
Laboratory Strain ◽  
Specific Identification ◽  
The Common ◽  
Common Laboratory
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