Nutritional diversity of Brachiaria ruziziensis clones

ABSTRACT: The aim of this study was to evaluate the nutritional diversity of Brachiaria ruziziensis clones through chemical composition and in vitro kinetics of ruminal fermentation. Twenty three clones of Brachiaria ruziziensis were used (15, 16, 46, 174, 411, 590, 651, 670, 768, 776, 844, 859, 950, 965, 970, 975, 1067, 1093, 1296, 1765, 1806, 1894 and 1972) and Brachiaria ruziziensis cv. ‘Kennedy’, Brachiaria brizantha cv. ‘Marandu’ and Brachiaria decumbens cv. ‘Basilisk’ as controls within 27 days of harvesting. The experimental design used randomized blocks with 26 treatments (genotypes) and three replications. Evaluation of the nutritional divergence was performed using principal components analysis, based on the following discriminatory variables: in vitro dry matter digestibility (IVDMD), neutral detergent fiber (NDF), lignin, crude protein (CP), degradation rate of non-fibrous carbohydrates (KdNFC) and degradation rate of fibrous carbohydrates (KdFC). The evaluation of the nutritional diversity of Brachiaria genotypes was based on the two main components (IVDMD and NDF), which explains 96.2% of the total variance Variables of lower contribution to the discrimination of the clones were as degradation rates of the fibrous and non-fibrous carbohydrates. In the agglomerative hierarchical grouping analysis, five distinct groups were identified, where V group, formed by clones 46, 768 and 1067 have higher values of IVDMD compared to the other clones.

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In vitro ruminal undegradable proteins of alfalfa cultivars

Canadian Journal of Plant Science ◽

10.4141/p99-113 ◽

2000 ◽

Vol 80 (2) ◽

pp. 315-325 ◽

Cited By ~ 9

Author(s):

G. F. Tremblay ◽

R. Michaud ◽

G. Bélanger ◽

K. B. McRae ◽

H. V. Petit

Keyword(s):

Protein Degradation ◽

Dry Matter ◽

Degradation Rate ◽

Weight Ratio ◽

Principal Component ◽

Neutral Detergent Fiber ◽

Cultivar Differences ◽

Dry Matter Digestibility ◽

Protein Degradation Rate

The quality of alfalfa would be greatly improved by an increase in its ruminal undegradable protein (RUP) concentration. Protein degradation rate (PDR), in vitro dry matter digestibility (IVDMD), leaf weight ratio (LWR), dry matter yield (DMY), total nitrogen (TN), in vitro RUP (expressed on both TN, RUP-TN, and dry matter basis, RUP-DM), acid detergent fiber (ADF), and neutral detergent fiber (NDF) concentrations were determined in 27 alfalfa cultivars. Cultivars were seeded in triplicate on 2 consecutive years and evaluated during the 2 subsequent production years with two harvests per year. Protein degradation rate and RUP-TN were determined using a ruminal inhibitor in vitro system. Data were averaged for spring growth, summer regrowth, and both harvests across 2 production years. Each of the three data sets was analyzed by ANOVA followed by a principal component analysis (PCA) on the ANOVA means. For the four-harvest data, cultivar differences were highly significant (P < 0.001) for all variates except for PDR (P = 0.07) and RUP-TN concentration (P = 0.10). The first PCA axis was largely defined positively by RUP-DM, IVDMD, TN, LWR, and RUP-TN, but negatively with ADF, NDF, PDR, and DMY. The second PCA axis defined a contrast between PDR versus RUP-TN, DMY, ADF, and NDF. Five cultivars were distinctive with high or low PCA scores in all three PCA. Rangelander and Heinrichs, along with Ultra, had low PDR; the first two cultivars had low DMY whereas Ultra was a medium-yielding cultivar. In contrast, Algonquin and Oneida VR had high PDR and medium DMY. While the first principal component (PC) indicated a general trend that low PDR and high RUP were associated with low-yielding cultivars, the second PC identified specific cultivars with both low PDR and high DMY. Therefore, selection for low PDR and high DMY is feasible. Key words: ruminal protein escape, dry matter digestibility, alfalfa

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Effects of Wormwood (Artemisia montana) Essential Oils on Digestibility, Fermentation Indices, and Microbial Diversity in the Rumen

Microorganisms ◽

10.3390/microorganisms8101605 ◽

2020 ◽

Vol 8 (10) ◽

pp. 1605

Author(s):

Seong Shin Lee ◽

Dong Hyeon Kim ◽

Dimas Hand Vidya Paradhipta ◽

Hyuk Jun Lee ◽

Hee Yoon ◽

...

Keyword(s):

Microbial Diversity ◽

Short Chain Fatty Acid ◽

Rumen Fluid ◽

Neutral Detergent Fiber ◽

In Vitro Digestibility ◽

Ruminal Fermentation ◽

Dry Matter Digestibility ◽

Different Doses ◽

Better Than

This study investigated the effects of essential oil (EO) from three Korean wormwood (Artemisia Montana) plants on in vitro ruminal digestibility, fermentation, and microbial diversity. Dried (0.5 g) soybean meal (SBM) or bermudagrass hay (BGH) were incubated in buffered rumen fluid (40 mL) for 72 h with or without EO (5 mg/kg) from Ganghwa (GA), Injin (IN), or San (SA) wormwood (Experiment 1). Both SA and IN improved (p < 0.05) dry matter digestibility (DMD) of BGH, while GA reduced (p < 0.05) total short-chain fatty acid of BGH and SBM. Besides, SA increased (p < 0.05) numbers of Ruminococcus albus and Streptococcus bovis in SBM. Experiment 2 examined different doses (0, 0.1, 1, and 10 mg/kg) of SA, the most promising EO from Experiment 1. Applying SA at 10 mg/kg gave the highest DMD (L; p < 0.01) and neutral detergent fiber (Q; p < 0.05) digestibility for BGH. Applying SA at 1 mg/kg gave the highest R. albus population (Q; p < 0.05) in SBM. Therefore, SA was better than GA and IN at improving rumen fermentation, and the 0.1 to 1 and 10 mg/kg doses improved ruminal fermentation and in vitro digestibility of SBM and BGH, respectively.

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Effects of tannins and Monensin in a feedlot diet on in vitro ruminal fermentation

Semina Ciências Agrárias ◽

10.5433/1679-0359.2019v40n6supl2p3223 ◽

2019 ◽

Vol 40 (6Supl2) ◽

pp. 3223

Author(s):

Hariany Ferreira Martello ◽

Nelcino Francisco de Paula ◽

Bruna Gomes Macedo ◽

Joanis Tilemahos Zervoudakis ◽

Danielle Dias Brutti ◽

...

Keyword(s):

Volatile Fatty Acid ◽

Dry Matter ◽

Low Dose ◽

Ammonia Concentration ◽

Gas Production ◽

Neutral Detergent Fiber ◽

Ruminal Fermentation ◽

Kinetics Parameters ◽

Dry Matter Digestibility

The objective of this study was to evaluate the effects of tannins versus Monensin on in vitro ruminal fermentation of a feedlot diet. The treatments were: control (no additives); low tannin (2 mg g DM-1); medium tannin (4 mg g DM-1), high tannin (6 mg g DM-1), and Monensin (0.02 mg g DM-1). The substrate was a feedlot diet composed by hay and concentrate (15:85 w/w; DM basis). Ruminal fluid was obtained from three rumen-cannulated male Santa Inês sheep. In vitro incubations were carried out during four consecutive weeks (run). Gas production (GP) was recorded at 1, 2, 3, 4, 5, 6, 8, 10, 12, 18, 24, 30, 36, 42, 48, 60, 72, 84, and 96 h of incubation. At 48 and 96 h, two bottles per treatment were withdrawn to measure pH, ammonia concentration (NH3), volatile fatty acid (VFA), in vitro dry matter digestibility (IVDMD), and in vitro neutral detergent fiber digestibility (IVNDFD). Addition of tannin or Monensin did not affect (P > 0.05) the kinetics parameters. Tannin supplementation reduced (P < 0.05) the GP at 24 h compared to Monensin. Addition of Monensin decreased (P < 0.05) IVDMD at 96 h and IVNDFD at 48 and 96 h compared to the control. The IVNDFD was lower (P < 0.05) with Monensin than with tannin at 48 and 96 h. The NH3 was lower (P < 0.05) with tannin compared with Monensin. By increasing tannin dosage, NH3 levels changed quadratically (P < 0.05). The inclusion of tannin in vitro reduced the NH3 concentration considerably when used in low dose.

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Application of Selected Inoculant Producing Antifungal and Fibrinolytic Substances on Rye Silage with Different Wilting Time

Processes ◽

10.3390/pr9050879 ◽

2021 ◽

Vol 9 (5) ◽

pp. 879

Author(s):

Seong-Shin Lee ◽

Jeong-Seok Choi ◽

Dimas Hand Vidya Paradhipta ◽

Young-Ho Joo ◽

Hyuk-Jun Lee ◽

...

Keyword(s):

Lactobacillus Plantarum ◽

Dry Matter ◽

Lactobacillus Brevis ◽

Neutral Detergent Fiber ◽

Dry Matter Digestibility ◽

Fiber Digestibility ◽

Aerobic Stability ◽

Commercial Inoculant

This research was conducted to determine the effects of selected inoculant on the silage with different wilting times. The ryes were unwilted or wilted for 12 h. Each rye forage was ensiled for 100 d in quadruplicate with commercial inoculant (Lactobacillus plantarum sp.; LPT) or selected inoculant (Lactobacillus brevis 100D8 and Leuconostoc holzapfelii 5H4 at 1:1 ratio; MIX). In vitro dry matter digestibility and in vitro neutral detergent fiber digestibility were highest in the unwilted MIX silages (p < 0.05), and the concentration of ruminal acetate was increased in MIX silages (p < 0.001; 61.4% vs. 60.3%) by the increase of neutral detergent fiber digestibility. The concentration of ruminal ammonia-N was increased in wilted silages (p < 0.001; 34.8% vs. 21.1%). The yeast count was lower in the MIX silages than in the LPT silages (p < 0.05) due to a higher concentration of acetate in MIX silages (p < 0.05). Aerobic stability was highest in the wilted MIX silages (p < 0.05). In conclusion, the MIX inoculation increased aerobic stability and improved fiber digestibility. As a result of the wilting process, ammonia-N in silage decreased but ruminal ammonia-N increased. Notably, the wilted silage with applied mixed inoculant had the highest aerobic stability.

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EFFECT OF STRUCTURAL AND CHEMICAL FACTORS OF FORAGES ON POTENTIALLY DIGESTIBLE FIBER, INTAKE, AND TRUE DIGESTIBILITY BY RUMINANTS

Canadian Journal of Animal Science ◽

10.4141/cjas88-088 ◽

1988 ◽

Vol 68 (3) ◽

pp. 787-799 ◽

Cited By ~ 13

Author(s):

V. GIRARD ◽

G. DUPUIS

Keyword(s):

Cell Wall ◽

Dry Matter ◽

Plant Cell Wall ◽

Neutral Detergent Fiber ◽

Optimum Number ◽

Apparent Digestibility ◽

Cool Season ◽

Dry Matter Digestibility ◽

True Digestibility

In view of the large variation found in plant cell wall digestibilities with ruminants, an attempt was made to group 124 feeds into different lignification classes (clusters) on the basis of chemical characteristics. Each feed cluster was described using a structural coefficient [Formula: see text] that related the potentially digestible fiber (PDF, %) to the ratio between lignin and cell wall volume. The optimum number of clusters was determined iteratively by performing a regression of the apparent digestibility of dry matter at maintenance level (DDM1, %) against the PDF and cell soluble (SOL, %) contents of feeds. The [Formula: see text] coefficients varied from 0.05 (grains, N = 13) to 1.85 (corn silage, N = 3) and increased with the maturity of the grasses from 0.88 (legumes, vegetative cool season grasses, N = 26) to 1.33 (mature, cool season grasses, N = 19). Predicted PDF were closely correlated (r > 0.9, P < 0.01) to in vitro cell wall disappearances (IVCWD). Apparently digestible cell wall in four grasses and four legumes increased linearly with 96-h IVCWD and standard error (SE) was similar to the SE of predicted apparent digestible SOL from SOL concentrations. Assuming that similarity between SE could be also observed in larger samples, PDF and SOL were used in summative equations to predict apparent dry matter digestibility. DDM1 discounted for intake (DDM1 – 4, %) was regressed against SOL and PDF concentrations of 87 feeds:[Formula: see text]with ds and df, the true digestibilities of SOL and PDF. Estimates of ds and df were 0.98 and 0.95 for a zero-production (maintenance) level of intake, and 0.91 and 0.79 for an intake level four times maintenance. Since the true digestibility of the PDF component was only 4% – 13% lower than that of the cell soluble component, the concentration of PDF in cell wall was the major determinant in the variation in apparent digestibility of forages. Key words: lignin, neutral detergent fiber, true digestibility, cluster analysis, feeds

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Production and quality of Tifton 85 pastures overseeded with black oat: effects of irrigation and nitrogen fertilization

Semina Ciências Agrárias ◽

10.5433/1679-0359.2018v39n5p2071 ◽

2018 ◽

Vol 39 (5) ◽

pp. 2071

Author(s):

João De Assis Farias Filho ◽

Fabiana Luiza Matielo de Paula ◽

Adalberto Luiz de Paula ◽

Wagner Paris ◽

Fabrício Ghinzelli ◽

...

Keyword(s):

Nitrogen Fertilization ◽

Dry Matter ◽

Irrigation System ◽

Neutral Detergent Fiber ◽

Lower Percentage ◽

Forage Production ◽

Dry Matter Digestibility ◽

Randomized Design ◽

Dead Material

The objective of this work was to evaluate the effects of irrigation and nitrogen fertilization on the bromatological quality, forage production, and botanical and structural composition of Tifton 85 (Cynodon sp.) pastures overseeded with black oat (Avena strigosa). Four treatments were evaluated in a 2 × 2 factorial scheme (irrigated and non-irrigated × fertilized and non-fertilized), with three replicates, in a completely randomized design. In irrigated paddocks, the irrigation system was activated when the soil matric potential reached a value equal to, or higher than, 10 kPa and 135 kg N ha-1 was applied to fertilized paddocks, divided into four applications. The forage mass pre- and post-grazing, total forage production, and the botanical, structural, and bromatological composition of the pastures were evaluated. No interaction was observed between the irrigation and nitrogen fertilization factors for any of the variables and no significant differences were observed in forage mass between pre- and post-grazing or in Tifton leaf and stem percentages. Nitrogen fertilization had a significant effect on forage production, which was 2626.41 kg dry matter (DM) ha-1 higher in fertilized pastures than in unfertilized pastures. In addition, fertilization resulted in a lower percentage of dead material (6.66%), higher percentage of oat leaves (30.84%), higher leaf:stem ratio (1:45), higher crude protein content (24.13%), lower levels of neutral detergent fiber (NDF) (64.57%) and acid detergent fiber (ADF) (32.86%), and higher in vitro dry matter digestibility (73.01%) than in unfertilized pastures. The use of irrigation did not influence total forage production, however, it resulted in pastures with lower NDF (65.97%) and ADF (33.54%), and higher in vitro dry matter digestibility (73.48%) than unirrigated pastures. Nitrogen fertilization produced improvements in pasture structure, associated with higher dry matter yield and bromatological quality, while irrigation only produced pastures with lower fiber content and greater digestibility.

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Pengaruh Inokulasi Lactobacillus plantarum dan Saccharomyces cerevisiae terhadap Fermentasi dan Kecernaan In Vitro Silase Kulit Buah Kakao

Buletin Peternakan ◽

10.21059/buletinpeternak.v40i2.9294 ◽

2016 ◽

Vol 40 (2) ◽

pp. 124

Author(s):

Muhammad Askari Zakariah ◽

Ristianto Utomo ◽

Zaenal Bachruddin

Keyword(s):

Mixed Culture ◽

Dry Matter ◽

Degradation Rate ◽

In Vitro Digestibility ◽

Ruminal Fermentation ◽

Rumen Ph ◽

Range Test ◽

Fibre Fraction

The objective of this study was to identify the effect of L. plantarum and S. cerevisiae mixed culture inoculation into cocoa pods silage on chemical composition and in vitro digestibility. The four treatments were: 1 kg freshly harvested cocoa pods without inoculants as control (K); K + L. plantarum (KLp); K + S. cerevisiae (KSc); and K + L. plantarum and S. cerevisiae mixture (KLp+Sc) 0.1% dry matter, Cassava meal were added in all treatments. Each treatment was replicated 3 times, and then fermented for 21 days. Parameters observed in current study were gas test production, ruminal fermentation parameter, and in vitro digestibility. The collected data were analyzed by one-way analysis of variance and followed by Duncan’s new Multiple Range Test for data with significant differences. Result showed that the mixed culture Lp+Sc inoculation increased (P<0.05) chemical quality of cocoa pods by reducing fibre fraction and increase NFE contents, increased degradation rate, degradation theory, reduced rumen pH, and propionate acid production, without affecting acetate to propionate ratio, microbial protein synthesis, and digestibility of cocoa pod silage.

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Nutritional Quality and In Vitro Rumen Fermentation Characteristics of Silage Prepared with Lucerne, Sweet Maize Stalk, and Their Mixtures

Agriculture ◽

10.3390/agriculture11121205 ◽

2021 ◽

Vol 11 (12) ◽

pp. 1205

Author(s):

Musen Wang ◽

Fujin Zhang ◽

Xinxin Zhang ◽

Ying Yun ◽

Lei Wang ◽

...

Keyword(s):

Organic Matter ◽

Nutritional Quality ◽

Dry Matter ◽

Rumen Fermentation ◽

Gas Production ◽

Neutral Detergent Fiber ◽

Ruminal Fermentation ◽

Fresh Matter ◽

In Vitro Rumen Fermentation

The objective of this work was to evaluate the pH, chemical composition, minerals, vitamins, and in vitro rumen fermentation characteristics of silage prepared with lucerne, sweet maize stalk (MS), and their mixtures. Freshly chopped lucerne and MS were combined in ratios of 100:0 (M0, control), 80:20 (M20), 60:40 (M40), 40:60 (M60), 20:80 (M80), and 0:100 (M100) on a fresh matter basis. Each treatment was prepared in triplicate, and a total of eighteen silos were fermented for 65 days. After 65 days of fermentation, the pH values in M0, M20, M40, M60, M80, and M100 silages were 5.47, 4.84, 4.23, 4.13, 3.79, and 3.61, respectively. As the MS proportion in the mixtures increased, silage K, Ca, P, Na, Fe, and Cu concentrations linearly decreased (p < 0.001) and so did vitamins B5 and K1 and α-tocopherol. In vitro rumen dry matter and organic matter degradability, pH, ammonia, total volatile fatty acid, and gas production linearly decreased (p < 0.01), while neutral detergent fiber concentration linearly increased (p < 0.001), with increasing proportion of MS. The in vitro dry matter and organic matter degradability rapidly decreased when the MS percentage was ≥60%. In conclusion, the M40 silage is the most suitable for livestock utilization in local forage production considering the balance of silage pH, nutritional quality, and in vitro ruminal fermentation characteristics.

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Dehydration curve, gas exchange, and nutritional value of Bermuda grass hay under different conditions of storage

Semina Ciências Agrárias ◽

10.5433/1679-0359.2019v40n5p1965 ◽

2019 ◽

Vol 40 (5) ◽

pp. 1965

Author(s):

Ivaldir William Junior Machado ◽

Marcela Abbado Neres ◽

Deise Dalazen Castagnara ◽

Caroline Daiane Nath ◽

Tatiana Garcia Diaz

Keyword(s):

Gas Exchange ◽

Nutritional Value ◽

Cynodon Dactylon ◽

Neutral Detergent Fiber ◽

Bermuda Grass ◽

Dry Matter Digestibility ◽

Grass Hay ◽

Storage Times ◽

And Storage

The aim of this study was to characterize the dehydration curve and, gas exchange of bermudagrass (Cynodon dactylon L.) as well as the nutritional value of hay under different conditions of storage. The dehydration curve was evaluated at 7 time point (0, 13, 18, 22, 37, 42, and 44 hours after cutting) until baling, using five replicates. The time 0 (zero) corresponded to the time before cutting, performed at 19:00 hours. The design was completely randomized with measures repeated over time. Gas exchanges was evaluated 0, 14, and 18 hours after cutting, using 10 replicates. The nutritional value of cowpea hay was evaluated in two open and closed environments (SO and CS), three storage times (30, 60, and 90 days), and five replications. Dehydration of hay was carried out for 44 hours, rapid rates of dehydration was observed in the leaves. Gas exchange was more intense before cutting and up to 14 hours after cutting. Crude protein (CP) content was higher at the time of cutting and decrease after storage. The in vitro dry matter digestibility (IVDMD) and neutral detergent fiber (NDF) content showed no variation between the sampling time and storage systems evaluated. Bermuda grass hay presents rapid dehydration and undergoes changes in the bromatological composition with storage time. Internal temperature of the hay bale did not exceed the ambient temperature.

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PRODUCTION AND NUTRITIVE VALUE OF CANARANA ERECTA LISA (Echinocloa piramidalis Lam.) IN RESPONSE TO HARVEST INTERVALS

Ciência Animal Brasileira ◽

10.1590/1809-6891v20e-52300 ◽

2019 ◽

Vol 20 ◽

Author(s):

Angela Maria de Vasconcelos ◽

Mauricio Cesio Araujo Dutra ◽

Robson Mateus Freitas Silveira ◽

Valdson José da Silva ◽

Luís Alfredo Pinheiro Leal Nunes ◽

...

Keyword(s):

Nutritive Value ◽

Block Design ◽

Neutral Detergent Fiber ◽

Experimental Unit ◽

Forage Production ◽

Dry Matter Digestibility ◽

Forage Digestibility ◽

Randomized Complete Block Design ◽

Harvest Intervals

Abstract The objective of this study was to compare and explain the variations in the production and nutritive value of canarana erecta lisa grass (Echinocloa pyramidalis Lam.) at different harvest intervals. The treatments corresponded to the cut intervals of 20, 27, 34, 41, 48, 55, 62, and 69 days. The experimental design was a randomized complete block design with three replications. Each experimental unit had 6m2 (3m ×2m). The variables studied included forage production, canopy height, and nutritive value o harvested forage. Forage production varied from 11517 to 12324 kg ha-1; Plant heights from 48.8 to 234 cm; Neutral detergent fiber (NDF) from 68.8% to 71.13%; Acid detergent fiber (ADF) from 36.20% to 44.15%; Crude protein (CP) from 11.43% to 5.85% and in “in vitro” dry matter digestibility (IVDMD) from 63.30% to 47.42%. These values indicated that the nutritive value of canarana erecta lisa was reduced at longer harvest intervals. It is recommended to harvest the grass at 35 days of regrowth favoring productivity, nutritive value and forage digestibility.

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