Proximate Composition, Phytochemical Analysis and in vivo Antioxidant Activity of Pomegranate Seeds (Punica granatum) in Female Albino Mice

Oxidative stress and inflammation are interlinked processes. The aim of the study was to perform a phytochemical analysis and to evaluate the antioxidant and anti-inflammatory activities of ethanolic Mahonia aquifolium flower (MF), green fruit (MGF), and ripe fruit (MRF) extracts. Plant extract chemical composition was evaluated by HLPC. A DPPH test was used for the in vitro antioxidant activity. The in vivo antioxidant effects and the anti-inflammatory potential were tested on a rat turpentine oil-induced inflammation, by measuring serum nitric oxide (NOx) and TNF-alpha, total oxidative status (TOS), total antioxidant reactivity (TAR), oxidative stress index (OSI), 3-nitrothyrosine (3NT), malondialdehyde (MDA), and total thiols (SH). Extracts were administrated orally in three dilutions (100%, 50%, and 25%) for seven days prior to inflammation. The effects were compared to diclofenac. The HPLC polyphenol and alkaloid analysis revealed chlorogenic acid as the most abundant compound. All extracts had a good in vitro antioxidant activity, decreased NOx, TOS, and 3NT, and increased SH. TNF-alpha was reduced, and TAR increased only by MF and MGF. MDA was not influenced. Our findings suggest that M. aquifolium has anti-inflammatory and antioxidant effects that support the use in primary prevention of the inflammatory processes.

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Antimicrobial and Hepatoprotective Potential of Pterospermum acerifollium leaves Extracts on Swiss albino mice

Journal of Drug Delivery and Therapeutics ◽

10.22270/jddt.v10i3.4032 ◽

2020 ◽

Vol 10 (3) ◽

pp. 170-174

Author(s):

Deepa Varandani ◽

Sharmishta Sekhar ◽

Suman Trivedi ◽

Megha Jha ◽

Sourabh Jain

Keyword(s):

Antimicrobial Activity ◽

Antioxidant Activity ◽

Hepatoprotective Activity ◽

Diffusion Method ◽

Antitubercular Drug ◽

Drug Induced ◽

Swiss Albino Mice ◽

Serum Biochemical ◽

Albino Mice

The objective of present study to investigate the hepatoprotective activity of hydro-alcohollic extract leaves of Pterospermum acerifollium against antitubercular drug induced liver damage in swiss albino mice and also performs antimicrobial activity by disc diffusion assay. Successive extractions was performed with different organic solvents viz; hydroalcohollic by cold maceration. The extract was analysed as antioxidant activity as a content of Total phenolic content, Total flavanoid content, Reducing power assay and DPPH Scavenging assay. Antimicrobial activity of methanolic extract was estimated by Agar well diffusion method. Antitubercular drug induced is used as toxicants in hepatoprotective studies in acute condition was analysed by serum biochemical estimations by AST, ALT, ALP and Total Bilirubin. In-vivo Antioxidant activity was performed by LPO, GSH, SOD and Catalase. During the collection of tissue for biochemical estimation piece of tissue cut and transferred for Histopathological estimation. The levels were measured and it indicated that the extract had significant antioxidant activity however the results obtained were dose dependent the higher the dose (400 mg/kg) the better activity. The extract administered at dose 400 mg/kg showed better activity. The treatment with hydroalcohollic extract of Pterospermum acerifillium reduced the elevated levels of SGOT, SGPT, ALP, TB and also reversed the hepatic damage towards normal which further supports the hepatoprotective activity. Keywords: Succesive extraction, In-vivo, Serum biochemical, Cold maceration

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In vivo immunomodulatory effects of the methanolic leaf extract of Gymnema sylvestre in Swiss albino mice

Archives of Biological Sciences ◽

10.2298/abs141027018a ◽

2015 ◽

Vol 67 (2) ◽

pp. 561-570 ◽

Cited By ~ 4

Author(s):

Laxmi Ahirwal ◽

Siddhartha Singh ◽

Manish Dubey ◽

Vandana Bharti ◽

Archana Mehta ◽

...

Keyword(s):

Oral Administration ◽

Leaf Extract ◽

Delayed Type Hypersensitivity ◽

Phytochemical Analysis ◽

Gymnema Sylvestre ◽

Secondary Antibody ◽

Swiss Albino Mice ◽

Flow Cytometric ◽

Albino Mice

In the present study we performed a comparative phytochemical analysis of the immunomodulating activities of the methanol leaf extract of Gymnema sylvestre (MLEGS) in Swiss albino mice. The phytochemical screening conducted on MLEGS revealed the presence of several phytoconstituents, including saponins, alkaloids, glycosides, phenols, tannins, and flavonoids. Immunomodulatory activities were determined by hemagglutination antibody (HA) titer and delayed-type hypersensitivity (DTH) tests for determining specific and non-specific immune responses. Flow cytometric techniques were performed for the estimation of B lymphocytes (CD3 and CD19) and Th2 cytokines (IL-2, IFN-? and IL-4). The response produced by oral administration of MLEGS elicited a significant reduction in a dose-related manner in the primary and secondary antibody response and DTH response. The response produced by oral administration of MLEGS elicited significant reduction in a dose-related manner in the primary and secondary antibody and DTH responses, with maximum reduction observed at 200 mg/kg-body wt. The maximal reductions in the production of CD3, CD19, IL-2, IFN-? and IL-4 were 31.59, 32.12, 29.51, 32.45 and 33.53%, respectively, at 200 mg/kg body weight. This study demonstrates that G. sylvestre exerts immunosuppressive effects on the components of the immune system of mice, and points to its significant immunomodulatory potential.

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Punica granatum (Pomegranate) activity in health promotion and cancer prevention

Oncology Reviews ◽

10.4081/oncol.2018.345 ◽

2018 ◽

Cited By ~ 12

Author(s):

Shahindokht Bassiri-Jahromi

Keyword(s):

Clinical Trial ◽

Health Promotion ◽

Antioxidant Activity ◽

Cancer Progression ◽

Cancer Cell Line ◽

Punica Granatum ◽

Potential Health ◽

Cancer Diseases

Cancer has become one of the most fatal diseases in most countries. In spite of the medical care developing, cancer still remains a significant problem. The majority of the cancers are resistant to treatment. Thus, the research for novel, more efficient and less side effect treatment methods continues. Pomegranate contains strong antioxidant activity, with potential health interests. Research concern in pomegranate is increasing because of their anticancer potential due to possess rich in polyphenols. We highlight the pomegranate potential health benefits and mechanism of cancer progression inhibition. Pomegranate has indicated antiproliferative, anti-metastatic and anti-invasive effects on different cancer cell line in vitro, in vivo and clinical trial. The aim of this review is to evaluate functional properties and the medical benifits of pomegranate against various cancer diseases. In addition, pomegranate properties in in vitro and in vivo experimental human and animal clinical trials and its future use are explored. The available data suggest that Punica granatum (pomegranate) might be used in the control and potential therapeutic for some disease conditions and benefits human health status. This review summarizes in vitro, in vivo and clinical trial studies highlighting the pomegranate role in prevent and treatment of breast, prostate, lung, colon, skin and hepatocellular cell cancers.

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Studies on Antioxidant Activity of Pomegranate (Punica granatum) Peel Extract Using in Vivo Models

Journal of Agricultural and Food Chemistry ◽

10.1021/jf0255735 ◽

2002 ◽

Vol 50 (17) ◽

pp. 4791-4795 ◽

Cited By ~ 186

Author(s):

Kotamballi N. Chidambara Murthy ◽

Guddadarangavvahally K. Jayaprakasha ◽

Ravendra P. Singh

Keyword(s):

Antioxidant Activity ◽

Punica Granatum ◽

In Vivo Models ◽

Peel Extract

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Phytochemical Screening, Antioxidant Activity and Inhibitory Potential of Five Kenyan Medicinal Plants

Journal of Advances in Medical and Pharmaceutical Sciences ◽

10.9734/jamps/2021/v23i330223 ◽

2021 ◽

pp. 8-17

Author(s):

Mukhwana Dennis Wafula ◽

Ayieko Cyrus ◽

Mweresa Collins ◽

Ingonga Johnstone ◽

Matoke-Muhia Damaris

Keyword(s):

Antioxidant Activity ◽

Antioxidant Activities ◽

Radical Scavenging ◽

Phytochemical Analysis ◽

Pharmacological Studies ◽

Croton Macrostachyus ◽

Inhibitory Potential ◽

Dpph Radical Scavenging

The present study was conducted to evaluate preliminary phytochemical analysis and in vitro antioxidant activities of five plants (Olea europaea, Kigelia africana, Terminalia mollis, Croton macrostachyus and Bridella micrantha extracts). The plants were collected from Baringo County in Kenya, dried, pulverized into fine powders and extracted using methanol. Phytochemical analysis showed the presence of alkaloids, aminoacids and proteins, flavonoids, saponins, steroids, tannins and triterpenoids. The root extracts were further investigated for their potential antioxidant activity by using radical scavenging DPPH (2, 2-Diphenyl-2-picryl-hydrazyl) technique. Methanol extract of roots from the plants showed significant differences in DPPH radical scavenging activities. The results were concluded that extracts have a more secondary metabolites and potential source of antioxidants, which is warranty to evaluate further in vivo pharmacological studies.

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Phytochemical analysis, antioxidant and antibacterial efficacy of methanol and hexane extract of Centella asiatica

BIBECHANA ◽

10.3126/bibechana.v18i2.30760 ◽

2021 ◽

Vol 18 (2) ◽

pp. 18-25

Author(s):

Shanta Pokhrel ◽

Prabha Neupane

Keyword(s):

Antioxidant Activity ◽

Methanol Extract ◽

Antioxidant Activities ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Centella Asiatica ◽

Salmonella Typhi ◽

Phytochemical Analysis ◽

Toxicological Studies

Centella asiatica is well known for its anti-inflammatory, anticancer, antioxidant, antimicrobial, analgesic, diuretic properties. Its hexane and methanol extracts were screened for the presence of phytoconstituents as well as their antibacterial and antioxidant activity. The phytochemical screening showed the presence of carbohydrate, glycosides, flavonoids, phenolic compound, alkaloids, and saponin. The methanol extract showed more effective antibacterial activity against salmonella typhi. Antioxidant activity of methanol extract was evaluated by 2, 2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity showed potent antioxidant activities with IC50 value 3.74 μg/mL slightly higher than standard ascorbic acid (IC50 = 2.576 μg/mL). The present study indicates that the tested plant can be an important source of antibacterial agents and recommends that the active phytoconstituents be isolated, identified, and screened individually for activities and also subjected further for in vivo and toxicological studies. BIBECHANA 18 (2) (2021) 18-25

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