scholarly journals The Combined Effect of Squid Pen Chitooligosaccharide and High Voltage Cold Atmospheric Plasma on the Quality of Asian Sea Bass Slices Inoculated with Pseudomonas aeruginosa

2020 ◽  
Vol 21 (01) ◽  
pp. 41-50
Author(s):  
Avtar Singh ◽  
Soottawat Benjakul ◽  
Oladipupo Odunayo Olatunde ◽  
Ahmet Faruk Yesilsu

The effects of chitooligosaccharide (COS) at different concentrations (0.05, 0.1 and 0.2%, w/w) without and with the application of high voltage cold atmospheric plasma (HVCAP) at different ratios of argon (Ar) and oxygen (O2), 90%Ar/10%O2 and 80%Ar/20%O2 on the growth of Pseudomonas aeruginosa inoculated (6 log CFU/mL) on Asian sea bass slices were monitored for 0 and 24 h incubation at 4ºC. Impact of COS on the changes in lipid and protein oxidation caused by HVCAP was also determined. All the HVCAP treated slices showed reduction in Pseudomonas count (PC) as compared to control (without any treatment) at both 0 and 24 h of incubation (p<0.05). However, decrease in PC was enhanced when HVCAP treated slices were combined with COS, particularly with higher O2 ratio (20%). COS inhibited lipid and protein oxidation of the slices treated with HVCAP as indicated by lower peroxide value, thiobarbituric acid reactive substances (TBARS) and total carbonyl content. For all treated slices, total volatile base nitrogen (TVB-N) was decreased at both incubation times, compared to the control. Micrograph analysis showed perforated, deformed and distorted cells of Pseudomonas after treatment alone or in combination. Therefore, COS could lower microbial load when combined with HVCAP and simultaneously could reduce the adverse impact of both protein and lipid oxidation, in slices treated with HVCAP.

LWT ◽  
2020 ◽  
Vol 118 ◽  
pp. 108697 ◽  
Author(s):  
Juan M. Pérez-Andrés ◽  
María de Alba ◽  
Sabine M. Harrison ◽  
Nigel P. Brunton ◽  
P.J. Cullen ◽  
...  

2018 ◽  
Vol 38 (2) ◽  
pp. 379-395 ◽  
Author(s):  
Russell S. Brayfield ◽  
Abhijit Jassem ◽  
Michael V. Lauria ◽  
Andrew J. Fairbanks ◽  
Kevin M. Keener ◽  
...  

2002 ◽  
Vol 49 (1) ◽  
pp. 43-49 ◽  
Author(s):  
Dorota Kulawiak-Gałaska ◽  
Michał Woźniak ◽  
Lucedio Greci

The results presented demonstrate the influence of aromatic indolinic aminoxyls: 1,2-dihydro-2-ethyl-2-phenyl-3H-indole-3-phenylimino-1-oxyl (IA-C2) and 1,2-dihydro-2-octadecyl-2-phenyl-3H-indole-3-phenylimino-1-oxyl (IA-C18) on oxidation of lipids and proteins of cardiac sarcoplasmic reticulum membranes. We have used doxorubicin and t-butyl hydroperoxide as agents inducing oxidative stress in isolated rat cardiac sarcoplasmic reticulum membrane system. Carbonyl groups were measured as the end product of membrane protein oxidation, and thiobarbituric acid reactive substances were assessed as a marker of lipid peroxidation. Inhibition of peroxidation of certain membrane components depends on the length of acyl chain. Aminoxyl IA-C2 inhibits the lipid peroxidation process while IA-C18 is an efficient protector against protein oxidation.


2020 ◽  
Vol 15 (2) ◽  
pp. 115-125 ◽  
Author(s):  
Liyun Wang ◽  
Chuankai Xia ◽  
Yajun Guo ◽  
Chunjun Yang ◽  
Cheng Cheng ◽  
...  

Aim: The global of spread multidrug-resistant Pseudomonas aeruginosa has become a public health threat. Cold atmospheric plasma (CAP) is reported to have bactericidal efficacy; however, its effects on clinical super multidrug-resistant P. aeruginosa are unclear. The aim of this study was to investigate the bactericidal efficacy of CAP on a strain of super multidrug-resistant P. aeruginosa. Materials & methods: The effects of CAP treatments were evaluated using assays for the detection of growth, viability, metabolism, virulence factors and reactive oxygen species (ROS) levels. Results: Both CAP treatments dose-dependently inhibited cell viability and metabolic activity, and decreased the expression of several virulence factors. CAP treatment induced a significant increase in intracellular ROS levels, and ROS scavengers inhibited this effect. Conclusion: CAP treatment is a promising option for the clinical inhibition of multidrug-resistant P. aeruginosa, and the development of CAP technologies might be the key to solving the long-standing problem of drug-resistant bacteria.


Foods ◽  
2021 ◽  
Vol 10 (12) ◽  
pp. 2981
Author(s):  
Sanjun Jin ◽  
Hao Yang ◽  
Fangju Liu ◽  
Qian Pang ◽  
Anshan Shan ◽  
...  

This study aimed at examining the effects of curcumin supplementation on growth performance, antioxidant capacity, and meat quality of ducks. To investigate these effects, 600 healthy ducks were randomly assigned to four treatment groups with 10 replicates pens, and each pen contained 15 ducks. Ducks were fed a diet containing curcumin at levels of 0, 300, 400, and 500 mg kg−1 in different groups. The results demonstrated that curcumin supplementation is beneficial to the growth performance (p < 0.05) of ducks and antioxidant capacity (p < 0.05) of duck meat. In addition, dietary curcumin raised the meat quality of ducks, improving the meat color, increasing water-holding capacity, and inhibiting lipid and protein oxidation. In conclusion, the present study provides important insights into both the nutrient and qualities of ducks, finding that a dietary inclusion of 400–500 mg/kg of curcumin (kg−1) has the greatest effect.


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