scholarly journals Isoenzyme Expression in Bean Seed Germination Treated with Thiamethoxam with and without Drought Stress

2015 ◽  
Vol 06 (19) ◽  
pp. 3157-3163
Author(s):  
Arthur Blois Villela ◽  
Andreia da Silva Almeida ◽  
César Iván Suárez Castellanos ◽  
Cristiane Deuner ◽  
Vanessa Nogueira Soares ◽  
...  
Agrologia ◽  
2018 ◽  
Vol 4 (2) ◽  
Author(s):  
Stela Wusono ◽  
John M Matinahoru ◽  
CMA Watimena

Swietenia mahagoni is a timber forestry plants have allelopathy toxic, can interfere with the growth of surrounding plants. This study aimed to determine the effects of extracts from various parts of Swietenia mahagoni on seed germination of green beans and corn. This research was conducted at the Laboratory of Silviculture, an extract from a litter, fresh leaves, bark and roots of the Swietenia mahagoni plant and given to the seed germination green beans and corn. The results showed that the extract of fresh leaves and roots Swietenia mahagoni inhibit seed germination green beans and corn, while the provision of litter no effect. Green bean seed has a higher durability of the maize seed to allelopathy of root Swietenia mahagoni.


2020 ◽  
Vol 109 ◽  
pp. 103650
Author(s):  
Shu Wang ◽  
Mei Wei ◽  
Bingde Wu ◽  
Huiyuan Cheng ◽  
Kun Jiang ◽  
...  

2019 ◽  
Author(s):  
Pincang Zhao ◽  
Shenglin Hou ◽  
xiufang guo ◽  
Junting Jia ◽  
Weiguang Yang ◽  
...  

Abstract Background Drought is one of the most serious factors limiting plant growth and production. Sheepgrass can adapt well to various adverse conditions, including drought. However, during germination, sheepgrass young seedlings are sensitive to these adverse conditions. Therefore, the adaptability of seedlings is very important for plant survival, especially in plants that inhabit grasslands or the construction of artificial grassland. Results In this study, we found a sheepgrass MYB-related transcription factor, LcMYB2 that is up-regulated by drought stress and returns to a basal level after rewatering. The expression of LcMYB2 was mainly induced by osmotic stress and was localized to the nucleus. Furthermore, we demonstrate that LcMYB2 promoted seed germination and root growth under drought and ABA treatments. Additionally, we confirmed that LcMYB2 can regulate LcDREB2 expression in sheepgrass by binding to its promoter, and it activates the expression of the osmotic stress marker genes AtDREB2A, AtLEA14 and AtP5CS1 by directly binding to their promoters in transgenic Arabidopsis. Conclusions Based on these results, we propose that LcMYB2 improves plant drought stress tolerance by increasing the accumulation of osmoprotectants and promoting root growth. Therefore, LcMYB2 plays pivotal roles in plant responses to drought stress and is an important candidate for genetic manipulation to create drought-resistant crops, especially during seed germination.


2020 ◽  
Author(s):  
R. Anupriya ◽  
Sugitha Thankappan ◽  
A. Senthil ◽  
D. Rajakumar ◽  
Geetha S ◽  
...  

Abstract Seed germination plays a critical role in determining rice productivity under drought stress. We evaluated 100 traditional rice landraces originated from different agro-ecological zones of Tamil Nadu along with drought- susceptible (IR 64) and drought- tolerant (IR 64 DRT) checks. Moisture stress was induced using PEG 6000 and screening done over a range of osmotic potentials (-) 10 bars, (-) 12.5 bars and (-)15 bars for a period of 5 d. Physio-morphological traits such as germination rate, survival per cent, root and shoot length, vigor index, RS ratio and relative water content (RWC) were assessed during early drought stress. We observed significant changes in the seed macromolecules, phytohormone levels (GA and IAA), osmolytes and antioxidant responses (catalase and superoxide dismutase) between drought stress and control treatments. Kuliyadichan registered significantly higher IAA and GA (44% and 35% respectively over drought tolerant check IR 64 DRT) at drought stress, whereas all the landraces showed an elevated catalase activity. In PC analysis, first three PCs captured 88.93% of the total variation; significant differences were detected among genotypes with respect to the studied parameters. Six traditional landraces such as Kuliyadichan, Rajalakshmi, Sabhagidhan, Nootripathu, Chandaikar and Mallikar were selected and their inherent drought tolerance was associated with metabolic responses viz., triggered hydrolytic enzyme activities, hormonal cross-talk, ROS signaling and catalase under drought stress compared to drought sensitive IR64. Hence, these genotypes can be used as potential donor candidates towards genetic improvement of drought tolerance in rice.


2020 ◽  
Vol 1578 ◽  
pp. 012209
Author(s):  
Te Xu ◽  
Kangning He ◽  
Hui Wang ◽  
Tan Zhang ◽  
Bo Chang

Genes ◽  
2020 ◽  
Vol 11 (4) ◽  
pp. 404 ◽  
Author(s):  
Muthusamy Muthusamy ◽  
Joo Yeol Kim ◽  
Eun Kyung Yoon ◽  
Jin A. Kim ◽  
Soo In Lee

Expansins are structural proteins prevalent in cell walls, participate in cell growth and stress responses by interacting with internal and external signals perceived by the genetic networks of plants. Herein, we investigated the Brassica rapa expansin-like B1 (BrEXLB1) interaction with phytohormones (IAA, ABA, Ethephon, CK, GA3, SA, and JA), genes (Bra001852, Bra001958, and Bra003006), biotic (Turnip mosaic Virus (TuMV), Pectobacterium carotovorum, clubroot disease), and abiotic stress (salt, oxidative, osmotic, and drought) conditions by either cDNA microarray or qRT-PCR assays. In addition, we also unraveled the potential role of BrEXLB1 in root growth, drought stress response, and seed germination in transgenic Arabidopsis and B. rapa lines. The qRT-PCR results displayed that BrEXLB1 expression was differentially influenced by hormones, and biotic and abiotic stress conditions; upregulated by IAA, ABA, SA, ethylene, drought, salt, osmotic, and oxidative conditions; and downregulated by clubroot disease, P. carotovorum, and TuMV infections. Among the tissues, prominent expression was observed in roots indicating the possible role in root growth. The root phenotyping followed by confocal imaging of root tips in Arabidopsis lines showed that BrEXLB1 overexpression increases the size of the root elongation zone and induce primary root growth. Conversely, it reduced the seed germination rate. Further analyses with transgenic B. rapa lines overexpressing BrEXLB1 sense (OX) and antisense transcripts (OX-AS) confirmed that BrEXLB1 overexpression is positively associated with drought tolerance and photosynthesis during vegetative growth phases of B. rapa plants. Moreover, the altered expression of BrEXLB1 in transgenic lines differentially influenced the expression of predicted BrEXLB1 interacting genes like Bra001852 and Bra003006. Collectively, this study revealed that BrEXLB1 is associated with root development, drought tolerance, photosynthesis, and seed germination.


2020 ◽  
Vol 21 (21) ◽  
pp. 8258 ◽  
Author(s):  
Vishvanathan Marthandan ◽  
Rathnavel Geetha ◽  
Karunanandham Kumutha ◽  
Vellaichamy Gandhimeyyan Renganathan ◽  
Adhimoolam Karthikeyan ◽  
...  

Drought is a serious threat to the farming community, biasing the crop productivity in arid and semi-arid regions of the world. Drought adversely affects seed germination, plant growth, and development via non-normal physiological processes. Plants generally acclimatize to drought stress through various tolerance mechanisms, but the changes in global climate and modern agricultural systems have further worsened the crop productivity. In order to increase the production and productivity, several strategies such as the breeding of tolerant varieties and exogenous application of growth regulators, osmoprotectants, and plant mineral nutrients are followed to mitigate the effects of drought stress. Nevertheless, the complex nature of drought stress makes these strategies ineffective in benefiting the farming community. Seed priming is an alternative, low-cost, and feasible technique, which can improve drought stress tolerance through enhanced and advanced seed germination. Primed seeds can retain the memory of previous stress and enable protection against oxidative stress through earlier activation of the cellular defense mechanism, reduced imbibition time, upsurge of germination promoters, and osmotic regulation. However, a better understanding of the metabolic events during the priming treatment is needed to use this technology in a more efficient way. Interestingly, the review highlights the morphological, physiological, biochemical, and molecular responses of seed priming for enhancing the drought tolerance in crop plants. Furthermore, the challenges and opportunities associated with various priming methods are also addressed side-by-side to enable the use of this simple and cost-efficient technique in a more efficient manner.


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