scholarly journals Evaluating Human Sensory Perceptions and the Compartment Bag Test Assays as Proxies for the Presence and Concentration of Escherichia coli in Drinking Water in Western Kenya

2017 ◽  
Vol 97 (4) ◽  
pp. 1005-1008 ◽  
Author(s):  
Yolanda M. Brooks ◽  
Shalean M. Collins ◽  
Patrick Mbullo ◽  
Sera L. Young ◽  
Ruth E. Richardson ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Drinking Water ◽  
Western Kenya ◽  
Compartment Bag Test

Microbiological quality of residues from drinking water preparation

1995 ◽  
Vol 31 (5-6) ◽  
pp. 75-79 ◽  
Author(s):  
M. Würzer ◽  
A. Wiedenmann ◽  
K. Botzenhart
Keyword(s):  
Waste Water ◽  
Escherichia Coli ◽  
Pseudomonas Aeruginosa ◽  
Drinking Water ◽  
Water Treatment ◽  
Pathogenic Bacteria ◽  
Waste Water Treatment ◽  
Microbiological Quality ◽  
Detection Limits

In Germany the application of procedures such as flocculation and filtration in the preparation of drinking water results in the annual production of an estimated 500,000 t of sediments and sludges. Some of these residues have a potential for being reused, for example in agriculture, forestry, brickworks or waste water treatment. To assess the microbiological quality of residues from waterworks methods for the detection of enterobacteria, Escherichia coli, Salmonella, Pseudomonas aeruginosa, Legionella, poliovirus, Ascaris suis eggs and Cryptosporidium have been evaluated regarding their detection limits and were applied to various residues from German waterworks. Results show that sediments and sludges may contain pathogenic bacteria, viruses and protista. When residues from waterworks are intended to be reused in agriculture or forestry the microbiological quality should therefore be considered.

scholarly journals Factors associated with typical enteropathogenic Escherichia coli infection among children <5 years old with moderate-to-severe diarrhoea in rural western Kenya, 2008–2012

2020 ◽  
Vol 148 ◽  
Author(s):  
K. Fagerli ◽  
R. Omore ◽  
S. Kim ◽  
J. B. Ochieng ◽  
T. L. Ayers ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Severe Diarrhoea ◽  
Western Kenya ◽  
Factors Associated ◽  
Case Comparison ◽  
Escherichia Coli Infection ◽  
Logistic Regressions ◽  
Stool Specimens ◽  
Polymerase Chain

Abstract Typical enteropathogenic Escherichia coli (tEPEC) infection is a major cause of diarrhoea and contributor to mortality in children <5 years old in developing countries. Data were analysed from the Global Enteric Multicenter Study examining children <5 years old seeking care for moderate-to-severe diarrhoea (MSD) in Kenya. Stool specimens were tested for enteric pathogens, including by multiplex polymerase chain reaction for gene targets of tEPEC. Demographic, clinical and anthropometric data were collected at enrolment and ~60-days later; multivariable logistic regressions were constructed. Of 1778 MSD cases enrolled from 2008 to 2012, 135 (7.6%) children tested positive for tEPEC. In a case-to-case comparison among MSD cases, tEPEC was independently associated with presentation at enrolment with a loss of skin turgor (adjusted odds ratio (aOR) 2.08, 95% confidence interval (CI) 1.37–3.17), and convulsions (aOR 2.83, 95% CI 1.12–7.14). At follow-up, infants with tEPEC compared to those without were associated with being underweight (OR 2.2, 95% CI 1.3–3.6) and wasted (OR 2.5, 95% CI 1.3–4.6). Among MSD cases, tEPEC was associated with mortality (aOR 2.85, 95% CI 1.47–5.55). This study suggests that tEPEC contributes to morbidity and mortality in children. Interventions aimed at defining and reducing the burden of tEPEC and its sequelae should be urgently investigated, prioritised and implemented.

scholarly journals The Use of Lavender (Lavandula angustifolia) Essential Oil as an Additive to Drinking Water for Broiler Chickens and Its In Vitro Reaction with Enrofloxacin

Animals ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1535
Author(s):  
M. Adaszyńska-Skwirzyńska ◽  
D. Szczerbińska ◽  
S. Zych
Keyword(s):  
Escherichia Coli ◽  
Drinking Water ◽  
Body Weight ◽  
Essential Oil ◽  
Water Consumption ◽  
Broiler Chickens ◽  
Lavender Oil ◽  
Resistant Strains ◽  
Synergistic Reaction

Biological activity of lavender essential oil is a property that can potentially find an application in poultry nutrition. Nowadays, the use of bioactive compounds is encouraged in many areas of industry and agriculture, since these substances have similar properties as withdrawn antibiotic growth promoters. Additionally, antibiotic resistance bacteria are one of the most important current threats to animal health. The purpose of the study was to determine the influence of lavender essential oil on the production parameters and blood parameters in broiler chickens and to assess the lavender oil’s in vitro reaction in a combination with enrofloxacin towards Escherichia coli. One-day-old non-sexed chicks (Ross 308) were divided into three experimental groups, each consisting of 100 individuals (five replicate of 20 boiler chicken each). The chickens in the control group received drinking water with no addition of lavender essential oil. In the experimental groups, lavender oil was added to the drinking water at a concentration of 0.4 mL/L, in the LEO1–42 from 1 to 42 days of age and the LEO22–42 group from the 22 to 42 days of age. The chickens’ body weight, feed consumption, water consumption, deaths and elimination due to health reasons were determined in the experiment. On day 42 of the chickens’ lives, blood samples were collected based on which selected parameters were identified. An in vitro experiment of lavender oil in combination with enrofloxacin was investigated with a checkerboard method. The results of the experiment showed the antimicrobial and antioxidant activity of lavender essential oil and its positive effect on the production results of broiler chickens. The study results proved that the addition of lavender oil positively impacted the chickens’ final body weight and feed conversion ratio (p < 0.01). No differences were observed between the groups for water consumption, death rate and the examined biochemical and immunological blood serum indices. Lavender essential oil was demonstrated to increase the blood serum’s total antioxidant status. A synergistic reaction in vitro was observed for lavender oil combined with enrofloxacin against resistant strains of Escherichia coli. Based on our study, a health-promoting effect of adding LEO to water for broiler chickens was found. Moreover, in vitro studies indicate a significant effect of lavender essential oil on the inhibition of the resistant strains of Escherichia coli growth and synergistic reaction with enrofloxacin.

Evaluation of the inhibition of culturableEnterococcus faecium,Escherichia coli, orAeromonas hydrophiliaby an existing drinking water biofilm

2008 ◽  
Vol 7 (6) ◽  
pp. 559-568
Author(s):  
Shawn G. Gibbs ◽  
Mark C. Meckes ◽  
Melchor Ortiz ◽  
Christopher F. Green ◽  
Pasquale V. Scarpino
Keyword(s):  
Escherichia Coli ◽  
Drinking Water

scholarly journals Drinking water and diarrhoeal disease due to Escherichia coli

2003 ◽  
Vol 1 (2) ◽  
pp. 65-72 ◽  
Author(s):  
Paul R. Hunter
Keyword(s):  
Escherichia Coli ◽  
Drinking Water ◽  
Clinical Presentation ◽  
Diarrhoeal Disease ◽  
Central Place ◽  
E Coli ◽  
Related Disease ◽  
Direct Damage ◽  
Water Microbiology

Escherichia coli has had a central place in water microbiology for decades as an indicator of faecal pollution. It is only relatively recently that the role of E. coli as pathogen, rather than indicator, in drinking water has begun to be stressed. Interest in the role of E. coli as a cause of diarrhoeal disease has increased because of the emergence of E. coli O157:H7 and other enterohaemorrhagic E. coli, due to the severity of the related disease. There are enterotoxigenic, enteropathogenic, enterohaemorrhagic, enteroinvasive, enteroaggregative and diffusely adherent strains of E. coli. Each type of E. coli causes diarrhoeal disease through different mechanisms and each causes a different clinical presentation. Several of the types cause diarrhoea by the elaboration of one or more toxins, others by some other form of direct damage to epithelial cells. This paper discusses each of these types in turn and also describes their epidemiology, with particular reference to whether they are waterborne or not.

scholarly journals Spread of Antibiotic Resistance in Aquatic Environments: E. coli as a Case Study

Proceedings ◽  
2018 ◽  
Vol 2 (11) ◽  
pp. 693 ◽  
Author(s):  
Maria Adamantia Efstratiou ◽  
Marina Bountouni ◽  
Efthimios Kefalas
Keyword(s):  
Escherichia Coli ◽  
Drinking Water ◽  
Antibiotic Resistance ◽  
Rural Communities ◽  
Ground Water ◽  
Aquatic Environments ◽  
Multiple Resistance ◽  
E Coli ◽  
Of Greece

The aim of this study was to gather information on the spread of antibiotic resistance in Escherichia coli isolates from wells, boreholes and untreated drinking water in islands of Greece. We analyzed for antibiotic resistance 235 E. coli strains isolated from untreated drinking water of small rural communities, and ground water from 4 islands. Resistance was tested against Norfloxacin, Ciprofloxacin, Levofloxacin, Amoxicillin and Cefaclor. More than half (54.9%) were resistant to at least one of the antibiotics tested. Of these 26.3% showed multiple resistance (to two or more antibiotics). Strains from drinking water sources were overall more sensitive. Frequent resistance was observed for Amoxicillin (38.3%) and Levofloxacin (28.5%), low for Norfloxacin (5.5%).

scholarly journals Escherichia coli O157:H7 in Drin Prevalence of Escherichia coli O157:H7 in Drink from Different Food Premises in Kota Samarahan Sarawak

2019 ◽  
Vol 2 (2) ◽  
pp. a13-19
Author(s):  
ELEXSON NILLIAN ◽  
AMIZA NUR ◽  
DIYANA NUR ◽  
AMIRAH ZAKIRAH ◽  
GRACE BEBEY
Keyword(s):  
Escherichia Coli ◽  
Drinking Water ◽  
Coliform Bacteria ◽  
Most Probable Number ◽  
Plain Water ◽  
Escherichia Coli O157 ◽  
Probable Number ◽  
E Coli ◽  
Preventive Actions ◽  
Stx1 Gene

Contamination of drinks with E. coli O157:H7 served in food premises such as restaurants can cause haemorrhagic colitis and haemolytic uremic syndrome to humans. The presence or absence of faecal pathogen was demonstrated using coliform group as indicator microorganisms. Therefore, this study was conducted to detect the presence of E. coli O157:H7 in drinking water from food restaurant premise in Kota Samarahan and Kuching to ensure safe and potable drinking water is served to the consumer. A total of thirty (n=30) drink samples including six types of each of the samples are cold plain water, iced tea, iced milo, syrup and iced milk tea. Most Probable Number (MPN) procedure was used in this study to enumerate the MPN values of coliform bacteria in each drink collected. A total of 53.33% (16/30) of the drink samples showed positive E. coli detection. Then, the PCR assay showed 6.25% (one out of 16 isolates) samples were positive and carried stx1 gene produced by E. coli O157:H7 in iced milo sample types. This study showed the drinks collected from food premises was contaminated with faecal contamination, which was not safe to drink by the consumer. Therefore, preventive actions should be taken to prevent foodborne illness outbreak in future

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