A medium detecting β-glucuronidase for the simultaneous membrane filtration enumeration of Escherichia coli and coliforms from drinking water

1992 ◽  
Vol 15 (6) ◽  
pp. 273-276 ◽  
Author(s):  
D.P. Sartory ◽  
L. Howard
Keyword(s):  
Escherichia Coli ◽  
Drinking Water ◽  
Membrane Filtration

Comparison of three different media for the detection of E. coli and coliforms in water

2006 ◽  
Vol 54 (3) ◽  
pp. 141-145 ◽  
Author(s):  
C. Bernasconi ◽  
G. Volponi ◽  
L. Bonadonna
Keyword(s):  
Escherichia Coli ◽  
Drinking Water ◽  
Membrane Filtration ◽  
Simultaneous Detection ◽  
Alternative Methods ◽  
Phenotypic Characteristic ◽  
Iso Standard ◽  
E Coli ◽  
Microbiological Parameters ◽  
Filtration Technique

The European Drinking Water Directive defines reference methods for the enumeration of microbiological parameters in drinking water. The method to be used for Escherichia coli and coliforms is the membrane filtration technique on Lactose TTC agar with Tergitol 7. Many technical drawbacks of the procedure, as well as its limitations regarding the recent taxonomy of coliforms, make it necessary to evaluate alternative methods. Two alternative assays, a chromogenic media (m-ColiBlu24®) and a defined substrate technology-DST test (Colilert 18/Quanty Tray™) were compared with the ISO standard with attention to the phenotypic characteristic of the isolates. Results showed that the ISO method failed to detect an important percentage of coliforms and E. coli while m-ColiBlu24® and Colilert 18 provided results in a shorter time allowing the simultaneous detection of E. coli and coliforms with no further confirmation steps.

scholarly journals Taqman hydrolysis probe application for Escherichia coli, Salmonella enterica, and Vibrio cholerae detection in surface and drinking water

2019 ◽  
Vol 9 (3) ◽  
pp. 492-499
Author(s):  
Ahmed K. A. El-Sayed ◽  
Mohamed I. Abou-Dobara ◽  
Camelia A. Abdel-Malak ◽  
Amira A. E. El-Badaly
Keyword(s):  
Escherichia Coli ◽  
Drinking Water ◽  
Vibrio Cholerae ◽  
Salmonella Enterica ◽  
Membrane Filtration ◽  
Quantitative Polymerase Chain Reaction ◽  
Bacterial Count ◽  
Distribution Networks ◽  
E Coli ◽  
Taqman Qpcr

Abstract This study explored the applicability of using TaqMan qPCR (quantitative polymerase chain reaction) for Escherichia coli, Salmonella enterica and non-virulent Vibrio cholerae detection in surface and drinking water. One hundred and twenty water samples were collected monthly (January 2017–December 2017) from the surface water (input) and drinking water (output and distribution networks) of two drinking water treatment plants (DWTPs) in Damietta County, Egypt. The distribution of the studied bacteria based on their detection by TaqMan qPCR compared with membrane filtration (MF) technique showed that the higher positive samples were detected by TaqMan qPCR. The bacterial count was totally absent in all output samples. TaqMan qPCR assay (based on sequence detection of uidA, invA, and ompW) revealed 97.96%, 99.14%, and 98.3% specificity for E. coli, S. enterica, and non-virulent V. cholerae, respectively, compared with 100% specificity for all strains when MF cultures were applied. TaqMan qPCR exhibited 100% sensitivity for all strains, while it was 91.67%, 80%, and 50% using MF cultures for E. coli, S. enterica, and non-virulent V. cholerae, respectively. In conclusion, TaqMan qPCR sensitivity makes it a useful tool for urgent fast monitoring of water contamination, especially in network samples that contain low bacterial count.

scholarly journals A Syringe-Based Biosensor to Rapidly Detect Low Levels of Escherichia Coli (ECOR13) in Drinking Water Using Engineered Bacteriophages

Sensors ◽  
2020 ◽  
Vol 20 (7) ◽  
pp. 1953 ◽  
Author(s):  
Troy C. Hinkley ◽  
Spencer Garing ◽  
Paras Jain ◽  
John Williford ◽  
Anne-Laure M. Le Ny ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Drinking Water ◽  
Membrane Filtration ◽  
Genetically Engineered ◽  
World Health ◽  
Selective Enrichment ◽  
E Coli ◽  
Detection Assay ◽  
Colony Forming Units ◽  
Health Organization

A sanitized drinking water supply is an unconditional requirement for public health and the overall prosperity of humanity. Potential microbial and chemical contaminants of drinking water have been identified by a joint effort between the World Health Organization (WHO) and the United Nations Children’s Fund (UNICEF), who together establish guidelines that define, in part, that the presence of Escherichia coli (E. coli) in drinking water is an indication of inadequate sanitation and a significant health risk. As E. coli is a nearly ubiquitous resident of mammalian gastrointestinal tracts, no detectable counts in 100 mL of drinking water is the standard used worldwide as an indicator of sanitation. The currently accepted EPA method relies on filtration, followed by growth on selective media, and requires 24–48 h from sample to results. In response, we developed a rapid bacteriophage-based detection assay with detection limit capabilities comparable to traditional methods in less than a quarter of the time. We coupled membrane filtration with selective enrichment using genetically engineered bacteriophages to identify less than 20 colony forming units (CFU) E. coli in 100 mL drinking water within 5 h. The combination of membrane filtration with phage infection produced a novel assay that demonstrated a rapid, selective, and sensitive detection of an indicator organism in large volumes of drinking water as recommended by the leading world regulatory authorities.

scholarly journals Assessment of microbial contamination of drinking water with total coliform bacteria and Escherichia coli in the Bitola region

2019 ◽  
Vol 65 (2) ◽  
pp. 23-32
Author(s):  
Marta Nedelkova ◽  
Angela Delova ◽  
Tanja Petreska Ivanovska ◽  
Zoran Zhivikj ◽  
Lidija Petrushevska-Tozi
Keyword(s):  
Escherichia Coli ◽  
Drinking Water ◽  
Water Supply ◽  
Rural Areas ◽  
Membrane Filtration ◽  
Total Coliform ◽  
Coliform Bacteria ◽  
Quality And Safety ◽  
E Coli ◽  
Total Coliform Bacteria

The aim of this paper is to analyze the presence of Escherichia coli (E. coli) and total coliform bacteria (CB) in the drinking water of urban and rural areas of the Bitola region, as indicators for water quality and safety. All water in urban area is chlorinated, while at the same time the water in rural areas is non-chlorinated. The samples were analyzed according to the international standard method of membrane filtration MKC EN ISO 9308-1:2015. In all examined samples of drinking water in urban area, presence of E. coli and CB was not detected as a result of the disinfection of the water. On contrary, in all tested samples of the water from the rural water supply, presence of E. coli and CB was confirmed. Significant increase in coliform bacterial counts probably weather-related was found in the period from April to September. In addition, in the third quarter including July, August, and September, in many measurements, E. coli as an indicator of faecal contamination was identified in drinking water. In accordance with these findings and in order to provide safe drinking water, it is necessary to modernize the water supply for the population in rural areas, to disinfect permanently the drinking water and to apply regular laboratory controls which are a basic pre-condition. Otherwise, inappropriate management of the water systems can cause serious decrease in the quality and safety of the drinking water associated with an increased risk of appearance of the infectious diseases in people and hydric epidemic. Key words: drinking water, Escherichia coli, coliform bacteria, membrane filtration

scholarly journals Comparison of Membrane Filtration and Multiple-Tube Fermentation by the Colilert and Enterolert Methods for Detection of Waterborne Coliform Bacteria, Escherichia coli, and Enterococci Used in Drinking and Bathing Water Quality Monitoring in Southern Sweden

1998 ◽  
Vol 64 (8) ◽  
pp. 3079-3083 ◽  
Author(s):  
Karl F. Eckner
Keyword(s):  
Escherichia Coli ◽  
Drinking Water ◽  
Water Samples ◽  
Membrane Filtration ◽  
Water Quality Monitoring ◽  
Coliform Bacteria ◽  
Southern Sweden ◽  
Standard Methods ◽  
Bathing Water ◽  
Drinking Water Samples

ABSTRACT A total of 338 water samples, 261 drinking water samples and 77 bathing water samples, obtained for routine testing were analyzed in duplicate by Swedish standard methods using multiple-tube fermentation or membrane filtration and by the Colilert and/or Enterolert methods. Water samples came from a wide variety of sources in southern Sweden (Skåne). The Colilert method was found to be more sensitive than Swedish standard methods for detecting coliform bacteria and of equal sensitivity for detecting Escherichia coli when all drinking water samples were grouped together. Based on these results, Swedac, the Swedish laboratory accreditation body, approved for the first time in Sweden use of the Colilert method at this laboratory for the analysis of all water sources not falling under public water regulations (A-krav). The coliform detection study of bathing water yielded anomalous results due to confirmation difficulties. E. coli detection in bathing water was similar by both the Colilert and Swedish standard methods as was fecal streptococcus and enterococcus detection by both the Enterolert and Swedish standard methods.

scholarly journals Isolation of Multi Drug Resistant Escherichia coli from drinking water of Lahore City, Pakistan

2021 ◽  
Vol 15 (5) ◽  
pp. 1080-1083
Author(s):  
Nazia Mir ◽  
Sidrah Saleem ◽  
Muhammad Imran ◽  
Ayesha Ghazal ◽  
Muhammad Usman
Keyword(s):  
Escherichia Coli ◽  
Drinking Water ◽  
Membrane Filtration ◽  
Diffusion Method ◽  
Resistant Bacteria ◽  
Drug Resistant ◽  
Antibiotic Resistant ◽  
Acinetobacter Species ◽  
E Coli ◽  
Lahore City

Background: The major faecal coliform is Escherichia coli which contaminates the drinking water from human and animal faecal waste. In developing regions drinking water is a vital source of microbiological pathogens. Multi-drug resistant (MDR) is defined as resistance to one or more antibiotic groups. An E. coli count greater than 4/dl in municipal drinking water is indicative of unacceptable fecal contamination. Aim: To find out the frequency of MDR E. coli in water system of Lahore, Pakistan. Methodology: Drinking water was collected from different towns of Lahore. The samples were processed using Membrane Filtration Technique. In the present study, Multidrug Resistant E.coli was isolated and antibiotic resistant pattern was seen against 16 most commonly antimicrobials, using Kirby Bauer disc diffusion method. Results: Total 100 water samples were collected, frequency of Escherichia coliisolated was 27% and frequency of MDR E.coli was 19%. The highest resistance showed by the organism towards Ampicillin (AMP) 21(81.48%), Augmentin (AMC), and Ceftazidime (CAZ) 14(51.85%) respectively. In this study also frequency of Extended Spectrum β-Lactamases were seen. Most common organisms isolated were E. coli 7% and Klebsiella 5%. Frequency of other coliforms isolated from drinking water other than E. coli was Klebsiella species 26%, Pseudomonas species 27%, Enterobacter 7%, Citrobacter species 8% and Acinetobacter species 5%. Conclusion: This study revealed that drinking water of Lahore city is heavilycontaminated with pathogenic microorganisms. A high proportion of antibiotic resistant is due to overuse of antibiotics, in patients with mild infections and secretion of these resistant bacteria from patients to environment. One of the reasons could be the mixing of sewage lines with drinking water supply. So, there is solely requirement for further studies for the identification of the sources for these contaminants. Keywords: Isolates, E. coli, Klebsiella, Multi-drug resistant (MDR)

scholarly journals Occurrence of Escherichia Coli in Packaged Drinking Water Distributed in Katabi Sub County Uganda. A Cross Sectional Study.

2021 ◽  
Author(s):  
◽  
David Serunjogi ◽  
Marion Byonanebye ◽  
Emmanuel Kizza
Keyword(s):  
Escherichia Coli ◽  
Drinking Water ◽  
Membrane Filtration ◽  
Cross Sectional Study ◽  
Bottled Water ◽  
World Health ◽  
Sectional Study ◽  
Biochemical Tests ◽  
Cross Sectional ◽  
Sachet Water

Background: The world health organization (WHO) estimates that about a 1.1billion people drink unsafe water globally, and over 1.7 million deaths occur annually due to the consumption of water that is of faecal origin. Water in sachets is readily available and affordable but there is no concern about its safety. The purpose of this study was to assess the occurrence of Escherichia coli in packaged drinking water distributed in the Katabi sub country using the membrane filtration technique, isolation of organisms, and subjecting them to biochemical tests. The study was conducted in 2017. Methodology: A cross-sectional study design was used where Rwenzori, Riham water, Nevana, Highland, Blue wave, and sachet water were purposively selected in the main trading center within the Wakiso district. A total of 5 bottled water and 10 sachets of water were used. Duplicate sample (2) bottles of water from each were used. Therefore 30 samples of packaged water were used in this study. The bacteria were grown on media and confirmed using biochemical tests. Results: Sachet water contained pink lactose fermenting colonies on MacConkey culture media. Bottled water had no growth. The pink colonies were positive with Kligler iron agar (KIA), triple sugar iron agar (TSI), and sulphur and indole motility (SIM) positive. Conclusion and recommendation The majority of sachet water was contaminated with Escherichia coli an indicator of faecal contamination; and therefore, unsuitable for human consumption. The government of Uganda should carry out surveillance activities to enforce strict hygienic measures on sachet water producers and distributors.

Isolation of Multi Drug Resistant Escherichia coli from drinking water of Lahore City, Pakistan

2021 ◽  
Vol 15 (6) ◽  
pp. 1188-1191
Author(s):  
N. Mir ◽  
S. Saleem ◽  
M. Imran ◽  
A. Ghazal ◽  
M. Usman
Keyword(s):  
Escherichia Coli ◽  
Drinking Water ◽  
Membrane Filtration ◽  
Diffusion Method ◽  
Resistant Bacteria ◽  
Drug Resistant ◽  
Antibiotic Resistant ◽  
Acinetobacter Species ◽  
E Coli ◽  
Lahore City

Background: The major faecal coliform is Escherichia coli which contaminates the drinking water from human and animal faecal waste. In developing regions drinking water is a vital source of microbiological pathogens. Multi-drug resistant (MDR) is defined as resistance to one or more antibiotic groups. An E. coli count greater than 4/dl in municipal drinking water is indicative of unacceptable fecal contamination. Aim: To find out the frequency of MDR E. coli in water system of Lahore, Pakistan. Methodology: Drinking water was collected from different towns of Lahore. The samples were processed using Membrane Filtration Technique. In the present study, Multidrug Resistant E.coli was isolated and antibiotic resistant pattern was seen against 16 most commonly antimicrobials, using Kirby Bauer disc diffusion method. Results: Total 100 water samples were collected, frequency of Escherichia coliisolated was 27% and frequency of MDR E.coli was 19%. The highest resistance showed by the organism towards Ampicillin (AMP) 21(81.48%), Augmentin (AMC), and Ceftazidime (CAZ) 14(51.85%) respectively. In this study also frequency of Extended Spectrum β-Lactamases were seen. Most common organisms isolated were E. coli 7% and Klebsiella 5%. Frequency of other coliforms isolated from drinking water other than E. coli was Klebsiella species 26%, Pseudomonas species 27%, Enterobacter 7%, Citrobacter species 8% and Acinetobacter species 5%. Conclusion: This study revealed that drinking water of Lahore city is heavilycontaminated with pathogenic microorganisms. A high proportion of antibiotic resistant is due to overuse of antibiotics, in patients with mild infections and secretion of these resistant bacteria from patients to environment. One of the reasons could be the mixing of sewage lines with drinking water supply. So, there is solely requirement for further studies for the identification of the sources for these contaminants. Keywords: Isolates, E. coli, Klebsiella, Multi-drug resistant (MDR)

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