THE PRESENCE OF PLASMODIUM FALCIPARUM GAMETOCYTES IN HUMAN BLOOD INCREASES THE GRAVIDITY OF ANOPHELES GAMBIAE MOSQUITOES

2005 ◽  
Vol 73 (2) ◽  
pp. 312-320 ◽  
Author(s):  
HEATHER M. FERGUSON ◽  
PETER OBARE ◽  
LOUIS C. GOUAGNA ◽  
HAMZA BABIKER ◽  
ANDREW F. READ ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Anopheles Gambiae ◽  
Human Blood

scholarly journals Anopheles gambiae: Metabolomic Profiles in Sugar-Fed, Blood-Fed, and Plasmodium falciparum-Infected Midgut

2017 ◽  
Vol 2017 ◽  
pp. 1-49 ◽  
Author(s):  
Cody J. Champion ◽  
Phanidhar Kukutla ◽  
Elizabeth K. K. Glennon ◽  
Bo Wang ◽  
Shirley Luckhart ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Anopheles Gambiae ◽  
Human Blood ◽  
Blood Feeding ◽  
Dynamic Responses ◽  
Blood Digestion ◽  
Cellular Processes ◽  
Mosquito Midgut ◽  
Metabolite Profiles ◽  
Mass Spectrometry System

The mosquito midgut is a physiological organ essential for nutrient acquisition as well as an interface that encounters various mosquito-borne pathogens. Metabolomic characterization would reveal biochemical fingerprints that are generated by various cellular processes. The metabolite profiles of the mosquito midgut will provide an overview of the biochemical events in both physiological states and the dynamic responses to pathogen infections. In this study, the midgut metabolic profiles of Anopheles gambiae mosquitoes following feeding with sugar, human blood, mouse blood, and Plasmodium falciparum-infected human blood were examined. A mass spectrometry system coupled to liquid and gas chromatography produced a time series of metabolites in the midgut at discrete conditions (sugar feeding, 24 h and 48 h post-normal blood and P. falciparum-infected blood feeding). Triplicates were included to ensure system validity. A total of 512 individual compounds were identified; 511 were assigned to 8 superpathways and 75 subpathways. The dataset can be used for further inquiry into the metabolic dynamics of sugar and blood digestion and of malaria parasite infection. The dataset is accessible at the repository Dryad.

scholarly journals The early sporogonic cycle of Plasmodium falciparum in laboratory‐infected Anopheles gambiae : an estimation of parasite efficacy

1998 ◽  
Vol 3 (1) ◽  
pp. 21-28 ◽  
Author(s):  
Louis Clément Gouagna ◽  
Louis Clément Gouagna ◽  
Bert Mulder ◽  
Bert Mulder ◽  
Elisabeth Noubissi ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Anopheles Gambiae

scholarly journals Human biting rhythm of Anopheles gambiae Giles, 1902 (Diptera: Culicidae) and sleeping behaviour of pregnant women in a lagoon area in Southern Benin

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Armel Djènontin ◽  
Aziz Bouraima ◽  
Christophe Soares ◽  
Seun Egbinola ◽  
Gilles Cottrell
Keyword(s):  
Plasmodium Falciparum ◽  
Pregnant Women ◽  
Anopheles Gambiae ◽  
Early Morning ◽  
Allelic Frequency ◽  
Malaria Vectors ◽  
Mosquito Nets ◽  
Anopheles Gambiae Giles ◽  
Almost All ◽  
Southern Benin

Abstract Objective In the framework of EVALMOUS study aiming to assess the use and effectiveness of mosquito nets by pregnant women and other members of their household in a lagoon area in southern Benin, the behaviour of pregnant women relative to the time they go to bed using the net were recorded. Malaria vectors biting rhythm, Plasmodium falciparum infection and insecticide resistance genes in malaria vectors were also determined. Results Overall, 3848 females of Anopheles gambiae s. l were collected and 280 pregnant women responded to the survey. Almost all Anopheles gambiae s. l. tested were Anopheles coluzzi Coetzee and Wilkerson 2013 (Diptera: Culicidae). The CSP index in malaria vector was 1.85% and the allelic frequency of kdr gene was 74.4%. Around 90% of bites and Plasmodium falciparum Welch, 1897 (Haemosporida: Plasmodiidae) transmission occurred between 10 p.m. and 6 a.m., which coincides with the period when more than 80% of pregnant women were under bednet. Despite a slight early evening and early morning biting activity of malaria vectors in the study area, the good use of nets might remain a useful protection tool against mosquito biting and malaria transmission.

scholarly journals Effect of naturally occurring Wolbachia in Anopheles gambiae s.l. mosquitoes from Mali on Plasmodium falciparum malaria transmission

2017 ◽  
Vol 114 (47) ◽  
pp. 12566-12571 ◽  
Author(s):  
Fabio M. Gomes ◽  
Bretta L. Hixson ◽  
Miles D. W. Tyner ◽  
Jose Luis Ramirez ◽  
Gaspar E. Canepa ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Anopheles Gambiae ◽  
Malaria Transmission ◽  
Deleterious Effect ◽  
Plasmodium Falciparum Malaria ◽  
Anopheles Coluzzii ◽  
Naturally Occurring ◽  
Anopheles Gambiae S.L ◽  
Cat Fleas ◽  
Sporozoite Infection

A naturally occurring Wolbachia strain (wAnga-Mali) was identified in mosquitoes of the Anopheles gambiae complex collected in the Malian villages of Dangassa and Kenieroba. Phylogenetic analysis of the nucleotide sequence of two 16S rRNA regions showed that wAnga-Mali clusters with Wolbachia strains from supergroup A and has the highest homology to a Wolbachia strain isolated from cat fleas (Ctenocephalides). wAnga-Mali is different from two Wolbachia strains previously reported in A. gambiae from Burkina Faso (wAnga_VK5_STP and wAnga_VK5_3.1a). Quantitative analysis of Wolbachia and Plasmodium sporozoite infection in field-collected mosquitoes indicates that the prevalence and intensity of Plasmodium falciparum sporozoite infection is significantly lower in Wolbachia-infected females. The presence of Wolbachia in females from a laboratory Anopheles coluzzii (A. gambiae, M form) colony experimentally infected with P. falciparum (NF54 strain) gametocyte cultures slightly enhanced oocyst infection. However, Wolbachia infection significantly reduced the prevalence and intensity of sporozoite infection, as observed in the field. This indicates that wAnga-Mali infection does not limit early stages of Plasmodium infection in the mosquito, but it has a strong deleterious effect on sporozoites and reduces malaria transmission.

scholarly journals Merocyanine 540-sensitized photoinactivation of human erythrocytes parasitized by Plasmodium falciparum

Blood ◽  
1992 ◽  
Vol 80 (1) ◽  
pp. 21-24 ◽  
Author(s):  
OM Smith ◽  
SA Dolan ◽  
JA Dvorak ◽  
TE Wellems ◽  
F Sieber
Keyword(s):  
Stem Cells ◽  
Plasmodium Falciparum ◽  
Hematopoietic Stem Cells ◽  
Red Blood Cells ◽  
Human Blood ◽  
Blood Cells ◽  
Potential Usefulness ◽  
Hematopoietic Stem ◽  
Infected Blood ◽  
Merocyanine 540

The purpose of this study was to evaluate the photosensitizing dye merocyanine 540 (MC540) as a means for extracorporeal purging of Plasmodium falciparum-infected erythrocytes from human blood. Parasitized red blood cells bound more dye than nonparasitized cells, and exposure to MC540 and light under conditions that are relatively well tolerated by normal erythrocytes and normal pluripotent hematopoietic stem cells reduced the concentration of parasitized cells by as much as 1,000-fold. Cells parasitized by the chloroquine- sensitive HB3 clone and the chloroquine-resistant Dd2 clone of P falciparum were equally susceptible to MC540-sensitized photolysis. These data suggest the potential usefulness of MC540 in the purging of P falciparum-infected blood.

A comparison of malarial antigens from human and Aotus monkey blood infected with Plasmodium falciparum

Parasitology ◽  
1972 ◽  
Vol 64 (2) ◽  
pp. 191-195 ◽  
Author(s):  
R. J. M. Wilson ◽  
A. Voller
Keyword(s):  
Plasmodium Falciparum ◽  
Great Britain ◽  
Human Blood ◽  
Research Council ◽  
Medical Research Council ◽  
Financial Assistance ◽  
World Health ◽  
The World ◽  
Health Organization ◽  
Overseas Development

An extract of blood from Aotus monkeys infected with Plasmodium falciparum contained all the clones of malaria precipitins so far identified in human blood.We are grateful to the Medical Research Council and Overseas Development Administration of Great Britain and to the World Health Organization for their financial assistance.

scholarly journals A sensitive on‐chip probe–based portable nuclear magnetic resonance for detecting low parasitaemia plasmodium falciparum in human blood

2020 ◽  
Vol 3 (4) ◽  
Author(s):  
Manish Gupta ◽  
Kundan Singh ◽  
Daya Krishan Lobiyal ◽  
Cholakka Parambath Safvan ◽  
Bhuban Kumar Sahu ◽  
...  
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Plasmodium Falciparum ◽  
Magnetic Resonance ◽  
Human Blood ◽  
On Chip ◽  
Nuclear Magnetic

scholarly journals Plasmodium falciparum (Haemosporodia: Plasmodiidae) and O’nyong-nyong Virus Development in a Transgenic Anopheles gambiae (Diptera: Culicidae) Strain

2019 ◽  
Vol 56 (4) ◽  
pp. 936-941 ◽  
Author(s):  
John D Mumford ◽  
Carole A Long ◽  
Scott C Weaver ◽  
Katzutoyo Miura ◽  
Eryu Wang ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Anopheles Gambiae ◽  
Genetic Background ◽  
Homing Endonuclease ◽  
Virus Titer ◽  
Consistent Difference ◽  
Human Pathogens ◽  
Infectious Dose ◽  
Anopheles Gambiae Giles ◽  
Transgenic Mosquitoes

Abstract Transgenic Anopheles gambiae Giles (Diptera: Culicidae) mosquitoes have been developed that confer sexual sterility on males that carry a transgene encoding a protein which cuts ribosomal DNA. A relevant risk concern with transgenic mosquitoes is that their capacity to transmit known pathogens could be greater than the unmodified form. In this study, the ability to develop two human pathogens in these transgenic mosquitoes carrying a homing endonuclease which is expressed in the testes was compared with its nontransgenic siblings. Infections were performed with Plasmodium falciparum (Welch) and o’nyong-nyong virus (ONNV) and the results between the transgenic and nontransgenic sibling females were compared. There was no difference observed with ONNV isolate SG650 in intrathoracic infections or the 50% oral infectious dose measured at 14 d postinfection or in mean body titers. Some significant differences were observed for leg titers at the medium and highest doses for those individuals in which virus titer could be detected. No consistent difference was observed between the transgenic and nontransgenic comparator females in their ability to develop P. falciparum NF54 strain parasites. This particular transgene caused no significant effect in the ability of mosquitoes to become infected by these two pathogens in this genetic background. These results are discussed in the context of risk to human health if these transgenic individuals were present in the environment.

Sign in / Sign up

Export Citation Format

Share Document