scholarly journals Criblage phytochimique et activité antimicrobienne de six rhizomes comestibles utilisés en médecine traditionnelle à Lubumbashi (RDC)

2020 ◽  
Vol 14 (4) ◽  
pp. 1367-1380
Author(s):  
Valentin Chiribagula Bashige ◽  
Amuri Salvius Bakari ◽  
Philippe Ndjolo Okusa ◽  
Emery Mutombo Kalonda ◽  
Jean Baptiste Simbi Lumbu
Keyword(s):  
Antimicrobial Activity ◽  
Traditional Medicine ◽  
Trichophyton Rubrum ◽  
Imperata Cylindrica ◽  
Activité Antibactérienne ◽  
Dilution Method ◽  
Phytochemical Screening ◽  
Chemical Screening ◽  
Médecine Traditionnelle

Les affections microbiennes constituent des pathologies dominantes en RDC où pour y faire face la population recourt aux plantes. Cette étude a été conduite en vue d’évaluer l’activité antimicrobienne et identifier des groupes phytochimiques à potentiel antimicrobien de six rhizomes comestibles, utilisées en médecine traditionnelle à Lubumbashi. Le criblage phytochimique réalisé par des réactions classiques en solution a permis d’identifier dans tous les rhizomes, des flavonoïdes, des saponines, des tannins et dans 66,7% d’entre eux, des quinones et des terpénoïdes. La méthode de dilution a permis d’évaluer l’activité antibactérienne des extraits aqueux (EAQ) et méthanoliques (EME) qui ont tous été actif avec une intensité allant de très forte (CMI = 1,7 μg/mL) à moyenne (CMI = 50 μg/mL). Eriosema verdikii (EME : CMI = 1,7 μg/mL sur N. meningitidis et S. aureus) a présenté la meilleure activité antibactérienne et Imperata cylindrica (EAQ : CMI = 1,7 μg/mL sur Trichophyton rubrum), la meilleure activité antifongique. Le potentiel antimicrobien de ces rhizomes mis en évidence in vitro dans cette étude, fait des espèces étudiées des candidates à des investigations approfondies pouvant aboutir à la mise au point des médicaments traditionnels améliorés ou à la découverte de nouvelles molécules antibactériennes.Mots clés : antifongique, antibactérien, criblage chimique, rhizomes comestibles, Lubumbashi. English Title: Phytochemical Screening and Antimicrobial Activity of Six Edible Rhizomes Used in Traditional Medicine in Lubumbashi (DRC)Microbial infections are dominant pathologies in the DRC where, to deal with them, several people resort to plants used in traditional medicine. This assesses the antimicrobial activity of six edible rhizomes used in traditional medicine in the city of Lubumbashi (DRC) and identifies phytochemical groups. Phytochemical screening by solution reactions allowed the identification of flavonoids, saponins and tannins in all rhizomes, and in 66.7% of the rhizomes were found quinones and terpenoids. The dilution method allowed the evaluation of antibacterial activity, aqueous (EAQ) and methanolic (EME) extracts. This varies from very high (MIC = 1.7 μg / mL) to medium (MIC = 50 μg / mL). Eriosema verdikii (EME: MIC = 1.7 μg / mL on N. meningitidis and S. aureus) has the greatest antibacterial in the collection and Imperata cylindrica (EAQ: MIC = 1.7 μg / mL On Trichophyton rubrum), the greatest antifungal activity. The results obtained by this study show that the six rhizomes studied, along with their food virtues, possess antibacterial and antifungal properties that can explain their use in traditional medicine against various conditions; In addition, the various phytochemical groups within them would play an important role in the proven virtues.Keywords: antifungal, antibacterial, chemical screening, edible rhizomes, Lubumbashi  

scholarly journals Antimicrobial Evaluation of Caesalpinia decapetala

2018 ◽  
Vol 9 (12) ◽  
Author(s):  
Sharma V ◽  
Lobo R. ◽  
Singh G. ◽  
Chanana V. ◽  
Kalsi V. ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Methanolic Extract ◽  
Agar Medium ◽  
Dilution Method ◽  
Phytochemical Screening ◽  
In Vivo Evaluation ◽  
Crude Drug ◽  
Activity Evaluation

Objective: The present work is an attempt to assess the in vitro antimicrobial activity of the leaves of Caesalpinia decapetala (Roth) fabaceae family collected from forest area of Tamilnadu, India. Methods: The crude drug was successively extracted by Soxhlet assembly using Petroleum ether, dichloromethane, ethyl acetate and methanol as solvents. Preliminary phytochemical screening of different extracts was carried out using several colour and precipitative chemical reagents as per described methods. Antimicrobial activity of the extracts was evaluated against fungal strains (Aspergillus fumigatus and Candida albicans), Gram +ve bacteria (Staphylococcus aureus and Streptococcus pyogenes) and Gram –ve bacteria (Escherichia coli and Pseudomonas aeruginosa) using agar wells dilution method. Nutrient agar medium at37 oC and sabouraud dextrose e agar medium at 28 oC were used in antimicrobial activity evaluation and antifungal activity evaluation respectively. Results: Preliminary phytochemical screening of C. decepetala leaves showed the presence of alkaloids, glycosides, phenols, phytosterols, saponins and flavonoids crude drug. C. decapetala leaf extracts exhibited marked dose dependent antibacterial activity in vitro against tested bacteria. Methanolic extract was found to be more potent particularly against Staphylococcus aureus (Gram +ve bacteria) and staphylococcus aeruginosa (Gram -ve bacteria). Conclusion: Various phytochemicals were found to be present in C. decepetala leaves. Methanolic extract of C. decepetala leaves exhibited better antimicrobial activity in vitro and can be used as a good therapeutic approach for infectious disease management and therapy. Further studies on isolation of phyto-constituents and both in vitro and in vivo evaluation of pharmacological activities of isolated bioactive constituents of the crude drug are recommended as future works.

scholarly journals ANTIMICROBIAL ACTIVITY STUDY OF NEW QUINAZOLIN-4(3H)-ONES AGAINST STAPHYLOCOCCUS AUREUS AND STREPTOCOCCUS PNEUMONIAE

2021 ◽  
Vol 9 (4) ◽  
pp. 318-329
Author(s):  
M. A. Samotrueva ◽  
A. A. Ozerov ◽  
A. A. Starikova ◽  
N. M. Gabitova ◽  
D. V. Merezhkina ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Streptococcus Pneumoniae ◽  
Active Sites ◽  
Wide Spectrum ◽  
Experimental Studies ◽  
Amide Group ◽  
Pharmacological Effect ◽  
Dilution Method

Quinazolin-4(3H)-one derivatives exhibiting a wide spectrum of a pharmacological activity, represent a promising class of substances used to obtain antibacterial agents, which is especially important in the context of the emergence of pathogenic microorganisms’ resistance to drugs used in medicine. It has been proved that compounds having a naphthyl radical in the molecule, as well as an amide group bound to the benzene ring as quinazolinone substituents, are characterized by a pronounced antimicrobial activity against Staphylococcus aureus and Streptococcus pneumoniae.The aim of the research is a primary microbiological screening of the in vitro antimicrobial activity of new quinazolin-4(3H)-one derivatives against Staphylococcus aureus and Streptococcus pneumoniae, as well as the assessment of the relationship between the pharmacological effect and the structural transformation of the substance molecule, lipophilicity and the possibility of forming resistance to them.Materials and methods. The experimental studies have been carried out using well-known nosocomial pathogens of infectious and inflammatory diseases Staphylococcus aureus and Streptococcus pneumoniae by a serial dilution method.Results. A compound containing a naphthyl radical in its structure, which contributes to an increase in the hydrophobicity of the substance and its solubility in the membrane of a bacterial cell, has a bacteriostatic effect against both Staphylococcus aureus and Streptococcus pneumoniae. A similar pharmacological effect is exhibited by a derivative with an amide group as a substituent of the quinazolinone nucleus linked to a phenyl radical, which probably contributes to an increase in the degree of binding to active sites of enzymes involved in the DNA replication, and protein synthesis. Obviously, the increased lipophilicity, which promotes better binding to the efflux protein, cannot serve as objective characteristics of the emergence possibility of the pathogen’s resistance to this substance.Conclusion. Among the synthesized compounds, the leading substances that exhibit an antimicrobial activity against Staphylococcus aureus and Streptococcus pneumonia, have been identified. The assessment of the chemical structure made it possible to substantiate their pharmacological action and draw conclusions about the possibility of developing resistance to it in microbial cells.

scholarly journals Cytotoxic, antioxidant, and antimicrobial activities of Celery (Apium graveolens L.)

2021 ◽  
Vol 17 (1) ◽  
pp. 147-156
Author(s):  
Mohammed Saleh Al Aboody ◽  
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Antihypertensive Agents ◽  
Apium Graveolens ◽  
Cell Protein ◽  
Dilution Method ◽  
Local Market ◽  
Antioxidant And Antimicrobial Activity

Celery (Apium graveolens Linn, Family: Apiaceae) is a common edible herb used as a spice in the traditional medicine of several nations since time immemorial. The whole plant is extensively used in cooking as soups and salads. A. graveolens has various pharmacological properties such as anticancer, anti-obesity, anti-hepatotoxic, and antihypertensive agents. Hence, it is of interest to document the in vitro cytotoxic, antioxidant, and antimicrobial activity of A. graveolens. The plants were collected in the local market, shade dried, and different parts of the plants were extracted with 70% ethanol using a cold maceration process. Antioxidant tests were performed based on the various radical scavenging methods. Antimicrobial activity and MIC were completed using the respective cup-plate and two-fold serial dilution method. In vitro cytotoxic studies were achieved by the MTT; Sulphorhodamine B assayed total cell protein content. DLA and ESC cells determined the short-term toxicity. The leaf extract exhibited significant antioxidant properties against NO, DPPH, ABTS, LPO, and HPO methods. Thus, potential inhibition against Gram-positive, Gram-negative, and fungal strains within the MIC ranges of 250-500 μg/ml was observed. All the extracts of the plant presented in the study revealed greater cytotoxicity effects against five respective cancer cell lines, L6, Vero, BRL 3A, A-549, L929, and L-929 with the ranging of 443-168.5 μg/ml. Thus, we show that A. graveolens possess a potential cytotoxic, antioxidant, and antimicrobial activity.

Phytochemical Screening and In Vitro Antibacterial Activity of Green Tea (Camellia Sinensis L) Extract Against Staphylococcus Epidermidis

2020 ◽  
Vol 4 (8) ◽  
pp. 261-266
Author(s):  
Hardian Bimanto ◽  
Yuyun Dwi Wahyuni ◽  
Diah Titik Mutiarawati ◽  
Lully Hanni Endarini
Keyword(s):  
Green Tea ◽  
Staphylococcus Epidermidis ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Dilution Method ◽  
Phytochemical Screening ◽  
Normality Test ◽  
In Vitro Antibacterial Activity ◽  
Green Tea Leaves

This study aimed to determine phytochemical screening and ethanol extract activity of the green leaves against Staphylococcus epidermidis. The microbial activity of the ethanol extract was carried out using the diffusion and dilution method with concentrations used of 1.5%, 2.5%, 3.5%, 4.5%, and 5.5% accompanied by three repetitions. The results showed that the ethanol extract of green tea leaves contained alkaloids, saponins, steroids or triterpenoids, flavonoids, polyphenols, and tannins and had the largest inhibition zone diameter of 30mm at a concentration of 4.5%. KHM (Minimum Inhibitory Content) at a level of 4.5% and KBM (Minimum Kill Rate) at a concentration of 5.5%. Analysis of the data with the normality test then proceed with the Kruskal Wallis test obtained a value of P = 0.008. With the value P

scholarly journals New Poly(Propylene Imine) Dendrimer Modified with Acridine and Its Cu(II) Complex: Synthesis, Characterization and Antimicrobial Activity

Materials ◽  
2019 ◽  
Vol 12 (18) ◽  
pp. 3020 ◽  
Author(s):  
Paula Bosch ◽  
Desislava Staneva ◽  
Evgenia Vasileva-Tonkova ◽  
Petar Grozdanov ◽  
Ivanka Nikolova ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Copper Ions ◽  
Spectral Characteristics ◽  
Dilution Method ◽  
Pathogenic Microorganisms ◽  
Paramagnetic Resonance ◽  
Poly Propylene ◽  
New Compounds ◽  
First Time

A second-generation poly(propylene imine) dendrimer modified with acridine and its Cu(II) complex have been synthesized for the first time. It has been found that two copper ions form complexes with the nitrogen atoms of the dendrimeric core by coordinate bonds. The new compounds have been characterized by nuclear magnetic resonance (NMR), electron paramagnetic resonance (EPR), fourier-transform infrared spectroscopy (FTIR) and fluorescence spectroscopy. The spectral characteristics of the modified dendrimer have been measured in different organic solvents, and a negative fluorescence solvatochromism has been observed. The antimicrobial activity of the dendrimers has been tested against model pathogenic microorganisms in agar and by broth dilution method. The cotton fabric treated with both dendrimers has been evaluated towards pathogenic microorganisms. The obtained modified cotton fabrics have been shown to hamper bacterial growth and to prevent biofilm formation. Dendrimer cytotoxicity has been investigated in vitro in the model HEp-2 cell line.

scholarly journals Approach for the Synthesis of Potent Antimicrobials Containing Pyrazole, Pyrimidine and Morpholine Analogues

2016 ◽  
Vol 69 ◽  
pp. 87-96 ◽  
Author(s):  
Nisheeth C. Desai ◽  
Bonny Y. Patel ◽  
Bharti P. Dave
Keyword(s):  
Antimicrobial Activity ◽  
Dilution Method ◽  
Mass Spectral ◽  
H Nmr ◽  
Sar Studies ◽  
Mueller Hinton ◽  
Morpholine Derivatives ◽  
Mueller Hinton Broth ◽  
C Nmr

The present study is in the interest of some synthesized novel derivatives containing 4-(1,3-diphenyl-1H-pyrazol-4-yl)-N-(morpholinomethyl)-6-arylpyrimidin-2-amines pooled with different bio-active heterocycles such as pyrazole, pyrimidine and morpholine derivatives. The structures of newly synthesized compounds were elucidated by IR, 1H NMR, 13C NMR and mass spectral data. The synthesized compounds were evaluated for their in vitro antimicrobial activity against different bacterial and fungal strains using Mueller-Hinton Broth dilution method. On the basis of SAR studies, it was observed that the presence of electron withdrawing groups remarkably enhanced the antimicrobial activity of synthesized compounds.

scholarly journals Phytochemical Screening and Antimicrobial Activity of Bundung Plants Extract by Dilution Method

2019 ◽  
Vol 5 (1) ◽  
pp. 143-154
Author(s):  
Noval Noval ◽  
Iwan Yuwindry ◽  
Dahlia Syahrina
Keyword(s):  
Antimicrobial Activity ◽  
Secondary Metabolites ◽  
Inhibitory Activity ◽  
Screening Test ◽  
Ethanol Extract ◽  
Dilution Method ◽  
Phytochemical Screening ◽  
Extract Concentration ◽  
Convincing Data ◽  
Plants Extract

Bundung plants (Actinuscirpus Grossus) are widely spread in Borneo and used by society empirically as antimicrobials. Nevertheless, the use of Bundung plants as traditional medicine has not been equipped with convincing data, because there is no research that is related to the plants. In order to the use of the plants accountable, it is necessary to conduct research about phytochemical screening studies and tests the antimicrobial activity of ethanol extract of Bundung plants to staphylococcus aureusandEschericia Coli bacteria. Moreover, extraction does with maceration method. Secondary metabolite groups which are contained in the ethanol extract of Bundung plants were determined qualitatively using several of phytochemical reagents. The result of phytochemical screening test showed that ethanol extract of Bundung plants contains a group of secondary metabolites; namely flavonoid, tannin, saponin, phenolic, steroid and terpenoid. The method that was used to test antimicrobial activity to Staphylococcus aureusandEschericia Coli bacteria was a liquid dilution method with variations in extract concentration of 0,5%, 1%, 2%, 4% and 8% by considering at the clarity of each sample. The result of antimicrobial activity of liquid dilution to bacteria had inhibition at all concentrations and the biggest inhibitory activity was shown at concentration 8% with the clearest level and the MIC grade of the test is at a concentration of 1%. Based on the findings, it can be concluded that ethanol extract of Bundung plants has the potential of activities of an antimicrobial, especially from secondary flavonoid metabolites.

scholarly journals Synthesis and biological evaluation of heterocyclic 1,2,4-Triazole scaffolds as promising pharmacological agents

2020 ◽  
Author(s):  
Mukesh Kumari ◽  
Sumit Tahlan ◽  
Balasubramanian Narasimhan ◽  
Kalavathy Ramasamy ◽  
Siong Meng Lim ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Cell Lines ◽  
Biological Activities ◽  
Biological Evaluation ◽  
Dilution Method ◽  
Pharmacological Agents ◽  
Drug Candidates ◽  
Design And Synthesis ◽  
Hct 116

Abstract Background: Triazole is an important heterocyclic moiety that occupied a unique position in heterocyclic chemistry, due to its large number of biological activities. It exists in two isomeric forms i.e. 1,2,4-triazole and 1,2,3-triazole and used as core molecule for the design and synthesis of many medicinal compounds. 1,2,4-Triazole possess broad spectrum of therapeutically interesting drug candidates such as analgesic, antiseptic, antimicrobial, antioxidant, antiurease , anti-inflammatory, diuretics, anticancer, anticonvulsant, antidiabetic, antimigrain agents.Methods: The structure of all synthesized compounds were characterized by physicochemical properties and spectral means (IR and NMR). The synthesized compounds were evaluated for their in vitro antimicrobial activity against Gram-positive (B. subtilis), Gram-negative (P. aeruginosa and E. coli) bacterial and fungal (C. albicans and A. niger) strains by tube dilution method using ciprofloxacin, amoxicillin and fluconazole as standards. In-vitro antioxidant and anti-urease screening was done by DPPH assay and indophenol method, respectively. The in-vitro anticancer evaluation was carried out against MCF-7 and HCT116 cancer cell lines using 5-FU and cisplatin as standards.Results, discussion and conclusion: The biological screening results reveal that the compounds T5 (MICBS, EC = 24.7µM, MICPA, CA = 12.3 µM) and T17 (MICAN = 27.1µM) exhibited potent antimicrobial activity as comparable to standards ciprofloxacin, amoxicillin (MICCipro = 18.1µM, MICAmo = 17.1µM) and fluconazole (MICFlu = 20.4µM), respectively. The antioxidant evaluation showed that compounds T2 (IC50 = 34.83 µg/ml) and T3 (IC50 = 34.38 µg/ml) showed significant antioxidant activity and comparable to ascorbic acid (IC50 = 35.44 µg/ml). Compounds T3 (IC50 = 54.01µg/ml) was the most potent urease inhibitor amongst the synthesized compounds and compared to standard thiourea (IC50 = 54.25 µg/ml). The most potent anticancer activity showed by compounds T2 (IC50 = 3.84 μM) and T7 (IC50 = 3.25 μM) against HCT 116 cell lines as compared to standard 5-FU (IC50 = 25.36 μM).

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