Toxicological Evaluation of Ethanol Extract of Adenium obesum Stem Bark in African Catfish, Clarias gariepinus

Abstract Introduction: Histopathological effects of ethanol extract of Adenium obesum stem bark on the liver of Clariasgariepinus was investigated over a 96-h exposure period as an effective organic piscicide. Materials and Methods: An acute static toxicity bioassay was performed after a preliminary concentration range finding test was conducted. Results: Exposed fish showed adaptation behavioural changes with those of respiratory distress and abnormal nervous signs of toxicity, which was concentration-dependent. Mortality was recorded in some of the exposed fish while a median lethal concentration of 7.15 mgL-1 was established for the extract in the exposed fish. Non-significant (p>0.05) changes were recorded in serum aspartate aminotransferase, alanine aminotransferase and alkaline phosphatase activities of the exposed ish. Moderate liver damage was observed in the exposed fish based on the degree of tissue change (DTC) grading where significant (p<0.05) differences were observed between the DTC of the exposed groups and the control. Conclusion: Ethanol extract of A. obesum stem bark was toxic to the exposed fish with evidence of hepatotoxicity and therefore, can be used to eradicate the hardy C.gariepinus from aquatic environments.

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Gills and skin histopathological evaluation in African sharptooth catfish, Clarias gariepinus exposed to ethanol extract of Adenium obesum stem bark

The Egyptian Journal of Aquatic Research ◽

10.1016/j.ejar.2015.01.005 ◽

2015 ◽

Vol 41 (1) ◽

pp. 119-127 ◽

Cited By ~ 5

Author(s):

Samson Eneojo Abalaka ◽

Muhammad Yakasai Fatihu ◽

Najume Doguwar Giginya Ibrahim ◽

Suleiman Folorunsho Ambali

Keyword(s):

Clarias Gariepinus ◽

Ethanol Extract ◽

Stem Bark ◽

Histopathological Evaluation ◽

African Sharptooth Catfish

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Toxicological evaluation of the ethanol extract of Lophira alata (Ochnaceae) stem bark in rats

Journal of Pharmacy & Bioresources ◽

10.4314/jpb.v16i1.3 ◽

2019 ◽

Vol 16 (1) ◽

pp. 20

Author(s):

Loretta Oghenekome Iniaghe ◽

Elohor Emma Okpakpor

Keyword(s):

Ethanol Extract ◽

Stem Bark ◽

Toxicological Evaluation

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Introduction Pages

Notulae Scientia Biologicae ◽

10.15835/nsb11410626 ◽

2019 ◽

Vol 11 (4) ◽

pp. I-VIII

Author(s):

Radu E. SESTRAS

Keyword(s):

Oxidative Stress ◽

Clarias Gariepinus ◽

Reproductive Function ◽

Stem Bark ◽

Albino Rats ◽

African Catfish ◽

Ameliorative Effect ◽

Male Wistar Rats ◽

Rare And Endangered

Notulae Scientia Biologicae (http://www.notulaebiologicae.ro), Issue 4, Volume 11, 2019: The papers published in this issue represent interesting novelties in different topics of life science. Among the exciting researches, we invite readers to find news about: anticancer activity of water and methanol extracts of Hypericum scabrum L. on different cancer cell lines; curcumin alleviates potassium bromate-induced hepatic damage by repressing CRP Induction through TNF-α and IL-1β and by suppressing oxidative stress; evaluation of leukocyte mobilization and platelet aggregatory effects of ciprofloxacin, lincomycin and erythromycin in Wistar albino rats; ameliorative effect of aqueous leaf extract of Moringa oleifera on reproductive function following cadmium chloride induced oxidative stress in male Wistar rats; acute and sub-acute toxicity of Cola millenii leaf and stem bark extracts in albino rats; the effects of using synthetic and natural color foods on lipid profile and liver function in rats; phylogenetic analysis and in silico characterization of cytochrome P450 1A (Cyp1A) protein from the African catfish, Clarias gariepinus; growth performance indices of New Zealand white rabbits administered Panax ginseng extracts; the clinicopathological evaluations of the efficacy of incorporation of amantadine hydrochloride in the treatment of West African dwarf goats experimentally infected with peste des petits ruminants virus; in vitro plant regeneration from mature seed explants of Withania somnifera, an important, rare and endangered medicinal plant.

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Toxicological evaluation of methanol extract of Khaya senegalensis leaves in African catfish Clarias gariepinus

Journal of Applied Sciences and Environmental Management ◽

10.4314/jasem.v19i3.27 ◽

2015 ◽

Vol 19 (3) ◽

pp. 557

Author(s):

OA Abui ◽

MM Motouke

Keyword(s):

Methanol Extract ◽

Clarias Gariepinus ◽

African Catfish ◽

Toxicological Evaluation ◽

Khaya Senegalensis

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EFEKTIVITAS ANTIBAKTERI BAWANG PUTIH (Allium sativum L) SEBAGAI PENGAWET ALAMI PADA IKAN LELE DUMBO (Clarias gariephinus) SEGAR

Surya Medika: Jurnal Ilmiah Ilmu Keperawatan dan Ilmu Kesehatan Masyarakat ◽

10.32504/sm.v14i1.106 ◽

2019 ◽

Vol 14 (1) ◽

pp. 26

Author(s):

Dyah Anggraeni ◽

Nurlela Nurlela

Keyword(s):

Room Temperature ◽

Allium Sativum ◽

Research Result ◽

Clarias Gariepinus ◽

Storage Period ◽

The Body ◽

Garlic Extract ◽

African Catfish ◽

Natural Preservatives ◽

Colony Counter

Background: Natural preservatives are compounds produced by natural ingredients that can suppress bacterial growth and development. Natural preservatives are carried out because most of the preservatives circulating are chemicals and unsafe for the body. One of the natural preservatives is by using garlic extract (Allium sativum L). Objective: This study is aimed to determine the effectiveness of the antibacterial garlic (Allium sativum L) as a natural preservative in fresh African catfish (Clarias gariepinus). Method: This research used the Pour Plate iroculation method. African catfish (Clarias gariepinus) which is soaked with garlic (Allium sativum L) with a concentration of 7%, 14% and 21% for 30 minutes, then the fish will be kept at room temperature with a storage period of 24 hours and 48 hours and calculated growth in bacterial numbers with the Colony counter. Result: Based on the research result, it was found that garlic extract (Allium sativum L) can obstruct the effectiveness of antibacterial in African catfish (Clarias gariepinus) at a concentration of 14%.

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PEMBEKUAN SEMEN LELE DUMBO (Clarias Gariepinus BURCHELL 1822) SEBAGAI MODEL KRIOPRESERVASI SEMEN IKAN

Jurnal Natural ◽

10.30862/jn.v9i1.778 ◽

2010 ◽

Vol 9 (1) ◽

Author(s):

Lutfi Lutfi

Keyword(s):

Liquid Nitrogen ◽

Clarias Gariepinus ◽

African Catfish ◽

Nitrogen Vapor ◽

Dmso Concentration ◽

Freezing Temperatures ◽

Vapor Liquid

Effect of four types of diluentss and four concentration of DMSO (5%, 10%, 15% and 20%) against the motility of African catfish sperm were evaluated after storage at freezing temperatures. The steps in preparation the 16 treatments combination of the diluents are preparation of diluents, mixing diluents with DMSO, packing of semen into 0.3-ml straw, equilibration of semen at 4 °C for 30 minutes, freezing of semen in nitrogen vapor liquid at a height of 6.5 cm for 10 minutes, and subsequently storage of semen in liquid nitrogen (-196 0C) for further analysis of post-thawing motility (PTM). The result showed that the highest level of motility of spermatozoa was in treatment P1D15 (45.7 ± 4.3%) and the lowest was in treatment P2D20 (14.5 ± 13.2%). The best diluent in this observation was diluents containing NaCl, KCl, CaCl2 and NaHCO3. The best concentration was DMSO 15%. While the best interaction between the concentration of DMSO diluents is P1D15 treatments containing NaCl, KCl, CaCl 2 and NaHCO3 with a combination of 15% DMSO concentration. The conclusion of the research is that diluents containing NaCl, KCl, CaCl2 and NaHCO3 with a combination of 15% DMSO concentration can be used in cryopreservation of African catfish semen.

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Vitamin E as antioxidant in female african catfish (Clarias gariepinus) exposed to chronic toxicity of atrazine

Egyptian Journal of Aquatic Biology and Fisheries ◽

10.21608/ejabf.2012.2127 ◽

2012 ◽

Vol 16 (2) ◽

pp. 83-98 ◽

Cited By ~ 10

Author(s):

Shadia Kadry ◽

Afnan Amer ◽

Mohamed Marzouk ◽

Magdy Hanna ◽

Amal Azmy ◽

...

Keyword(s):

Vitamin E ◽

Chronic Toxicity ◽

Clarias Gariepinus ◽

African Catfish

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Evaluation of Toxicity and Antioxidant Property of Cassia fistula Stem Bark Extracts in Zebrafish

Journal of Health and Allied Sciences NU ◽

10.1055/s-0040-1715995 ◽

2020 ◽

Vol 10 (03) ◽

pp. 109-115

Author(s):

Shreya Udaya ◽

Nishith Babu ◽

Dechamma Pandyanda Nanjappa ◽

Krithika Kalladka ◽

Gunimala Chakraborty ◽

...

Keyword(s):

Aqueous Extract ◽

Antioxidant Properties ◽

Ethanol Extract ◽

Antioxidant Property ◽

Stem Bark ◽

Oxygen Species ◽

Cassia Fistula ◽

Potential Applications ◽

Ethanol Extracts ◽

Stress Models

Abstract Objective This study was aimed at evaluating the toxicity and the antioxidant property of Cassia fistula stem bark extracts in zebrafish. Materials and Methods Crude aqueous and ethanol extracts of C. fistula stem bark were obtained following a standard solvent-based extraction method. The toxicity of these extracts on zebrafish embryonic development was determined and the LC50 values were calculated. Finally, the antioxidant property of C. fistula stem bark extracts was determined in arsenic-induced oxidative stress models of zebrafish. Results The aqueous extract of C. fistula stem bark showed a slightly larger LC50 value (213.6 ppm) compared with the ethanol extract (LC50 = 63.5 ppm), suggesting a lower toxicity of the aqueous extract. A significant reduction of reactive oxygen species (ROS) signal was observed in arsenic-exposed embryos treated with the aqueous extract, but not the ethanol extract, indicating that the antioxidant activity is present only in the aqueous extract of C. fistula stem bark. Conclusion Identification of antioxidants from natural sources is desirable because of increasing safety concerns associated with synthetic antioxidants. This study demonstrated that aqueous extract from C. fistula stem bark possesses antioxidant properties, which can be further characterized for mechanism of action and potential applications.

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