scholarly journals Identification of Fungi Associated with Irvingia gabonensis (Ogbono) Seeds Spoilage in Benin City, Nigeria

2021 ◽  
Vol 25 (5) ◽  
pp. 779-782
Author(s):  
B.O. Okobiebi ◽  
J.N. Ezennia

Irvingia gabonensis (Ogbono) is one of the forest tree species of great domestic importance and its postharvest shelve life is affected by various species of fungi. This study is to investigate the effect of various species of fungi that attack Irvingia gabonensis seeds in post-harvest. Ready to use cotyledons of Ogbono were obtained from six (6) markets in Benin City (New Benin market, Aduwawa market, Uselu market, Oba market, Ikpoba Hill market, Santana market) Edo State, in sterile transparent polyethylene bags and transported to the laboratory for analyses. The proximate composition of the Ogbono seeds was determined using standard protocols. The pour plate method was used for isolation using a potato Dextrose Agar which was supplemented with streptomycin. The fungi were isolated based on their cultural and microscopic characteristics. The result of the highest and lowest fungal counts were too numerous to count and 3.3 × 103 cfu/g respectively. A total of seven species of fungi were isolated and identified in this study which included Aspergillus flavus, Aspergillus fumigatus, Aspergillus niger, Mucor sp., Neurospora sp., Penicillium sp., and Rhizopus sp. Aspergillus flavus was the most prevalent fungi with prevalence rate of 48 (90.6%), while Neurospora sp. was the least prevalent fungi with prevalent rate of 04 (18.2%). The presence of these fungi especially Aspergillus species portrays a serious public health implication as regard food poisoning, mycotocosis and food security. Proper handling methods of Ogbono seeds, coupled with good orientation are necessary to ensure the safety of ogbono seeds.

2021 ◽  
Vol 2 (2) ◽  
pp. 45-54
Author(s):  
G. B. Popoola ◽  
F. I. Okungbowa

Global concern about food safety has led to increased interest in the study of food-spoilage fungi. Contaminated food affects human and animal health when such food items are consumed. This study was carried out to determine the fungi associated with stored corn in a bid to establishing their possible role in deterioration of corn. Using standard procedures, these fungi were isolated:  Aspergillus, Trichoderma, Penicillium, and Rhizopus species. Results revealed the production of enzymes (protease, amylase, and lipase) by the fungi. The activity of these enzymes could play a role in the spoilage of corn by the fungi.  In addition, the isolated fungi are known to be associated with postharvest yield losses in stored produce as well as producing toxins. Therefore, the data presented will help in choosing postharvest practices that will reduce contamination by these fungi. Protease activity values of the isolates ranged from 1.426±0.042 – 1.748±0.023µml-1 (in Aspergillus sp.), 1.599±0.018 – 1.990±0.019µml-1 (in Rhizopus sp.), 1.364±0.018 – 1.679±0.012µml-1 (in Trichoderma sp.) and (1.544±0.017 – 1.714±0.013 μml-1 (in Penicillium sp.). Amylase activity was highest in Rhizopus (1.625±0.054 – 1.790±0.013 μml-1), followed by Penicillium sp and lowest in Aspergillus sp. Lipase activity was highest in Penicillium and lowest in Aspergillus. The detection and identification of fungi are crucial to developing appropriate management strategies for stored corn.  Postharvest losses due to these fungi will lead to reduced income for the farmers.


2020 ◽  
Vol 36 (2) ◽  
pp. 21-26
Author(s):  
E.O. Oni ◽  
L.O. Adebajo ◽  
A.O. Badmos ◽  
T.M. Adeleye ◽  
F.M. Oyeyipo ◽  
...  

Fungi constitute a major problem in the production, storage and processing of agricultural products, recent concern about the consumption of stale retail pepper in Abeokuta necessitated the need to determine the fungal population and also to quantify Aflatoxin produced by the fungi. Fungi species were isolated from 20 pepper samples (Capsicum annum) bought from different markets in Abeokuta using standard microbiological procedures and High Performance Liquid Chromatography (HPLC) was used to quantify the aflatoxin present. Isolated fungi were Aspergillus flavus (55%), Mucor sp (10%), Sacharomyces cerevisiae (20%), Aspergillus fumigatus , Rhizopus sp and Penicillium sp (5%) respectively. Results shows that fourteen of the pepper samples had no detectable aflatoxin and the aflatoxin content in the remaining six samples was 23ng/kg, 18 ng/kg, 15 ng/kg, 9 ng/kg, 6 ng/kg and 2 ng/kg,. five of these samples had aflatoxin content above the European Union maximum tolerance level of 5ng/kg. The presence of toxin producing Aspergillus flavus capable of causing food poisoning raises concern over public health risks that may be associated with the consumption of stale pepper. Keywords: Aflatoxin, Fungal contamination, Capsicum annum, stale foods, High Performance Liquid chromatography, public health


Data in Brief ◽  
2018 ◽  
Vol 20 ◽  
pp. 101-107 ◽  
Author(s):  
Sheila A. Bishop ◽  
Hilary I. Okagbue ◽  
Olumuyiwa A. Oludayo ◽  
Olasunmbo O. Agboola ◽  
Michael C. Agarana ◽  
...  

2016 ◽  
Vol 43 (4) ◽  
pp. 258
Author(s):  
A.A. Okolo ◽  
R.I. Okonkwo ◽  
R.C. Ideh

2021 ◽  
Vol 2 (1) ◽  
pp. 25-42
Author(s):  
B. Ikhajiagbe ◽  
V. D. Odenore ◽  
E. O. Umeokeke ◽  
E. S. Ahanor ◽  
O. Amadin ◽  
...  

The influence of humans on biodiversity at the genetic, species, community, and ecosystem levels is enormous. This is even more impactful on plant populations, which, being immobile, are at the mercy of their immediate environment. Digitaria horizontalis is a very common grass species in Benin City. Its ruderal nature accounts mostly for its widespread. The current study investigated the existence of possible genetic diversity of the grass in built environment in Benin City. Plant samples were randomly collected from 21 built environment locations within 6 Local Government Council Areas that make up Benin City. Test plants were allowed to acclimatize in experimental bowls and then allowed to grow for 4 weeks after which morphological characteristics were determined. Genetic diversity was determined by Random Amplification of Polymorphic DNA. Significant differences in plant height (33.0 – 59.0 cm) occurred when plants were compared based on location of collection. Changes in patterns of the association with weeds within the vicinity of the test plant was observed. The most prevalent plant species associated with the test plant was Pennisetum purpureum with a 12.18% prevalence. RAPD analysis using primer OPA 04 revealed a locus (about 600bp) that was absent in one or more of the locations with minimum disturbance. This 600pb fragment was however observed in most samples from highly built up and trampled environments. Primer OPA 03 showed monomorphic bands in 2 test samples with a band size of 400bp. It is therefore reported that there is an existence of the genetic diversity in a landscape of grass species (Digitaria horizontalis) found in different built environment in Benin City, Edo State, Nigeria.


Author(s):  
Obi Collins Uchechukwu ◽  
Aladeyelu Okikioluwa Stephen ◽  
Onyejike Darlington Nnamdi ◽  
Arusiwon Joseph Akinwale ◽  
Agbiogwu Ijeoma Nnenna

2015 ◽  
Vol 14 (2) ◽  
pp. 110 ◽  
Author(s):  
EbeleG Abadom ◽  
BenedictaA Akpe ◽  
ErnestA Omoti

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