scholarly journals GENETIC DIVERSITY OF Digitaria horizontalis Willd. IN BUILT ENVIRONMENTS IN BENIN CITY, NIGERIA

2021 ◽  
Vol 2 (1) ◽  
pp. 25-42
Author(s):  
B. Ikhajiagbe ◽  
V. D. Odenore ◽  
E. O. Umeokeke ◽  
E. S. Ahanor ◽  
O. Amadin ◽  
...  

The influence of humans on biodiversity at the genetic, species, community, and ecosystem levels is enormous. This is even more impactful on plant populations, which, being immobile, are at the mercy of their immediate environment. Digitaria horizontalis is a very common grass species in Benin City. Its ruderal nature accounts mostly for its widespread. The current study investigated the existence of possible genetic diversity of the grass in built environment in Benin City. Plant samples were randomly collected from 21 built environment locations within 6 Local Government Council Areas that make up Benin City. Test plants were allowed to acclimatize in experimental bowls and then allowed to grow for 4 weeks after which morphological characteristics were determined. Genetic diversity was determined by Random Amplification of Polymorphic DNA. Significant differences in plant height (33.0 – 59.0 cm) occurred when plants were compared based on location of collection. Changes in patterns of the association with weeds within the vicinity of the test plant was observed. The most prevalent plant species associated with the test plant was Pennisetum purpureum with a 12.18% prevalence. RAPD analysis using primer OPA 04 revealed a locus (about 600bp) that was absent in one or more of the locations with minimum disturbance. This 600pb fragment was however observed in most samples from highly built up and trampled environments. Primer OPA 03 showed monomorphic bands in 2 test samples with a band size of 400bp. It is therefore reported that there is an existence of the genetic diversity in a landscape of grass species (Digitaria horizontalis) found in different built environment in Benin City, Edo State, Nigeria.

Author(s):  
Mohamed Abd. S. El zayat ◽  
Mahmoud El Sayd Ali ◽  
Mohamed Hamdy Amar

Abstract Background The Capparaceae family is commonly recognized as a caper, while Cleomaceae represents one of small flowering family within the order Brassicales. Earlier, Cleomaceae was included in the family Capparaceae; then, it was moved to a distinct family after DNA evidence. Variation in habits and a bewildering array of floral and fruit forms contributed to making Capparaceae a “trash-basket” family in which many unrelated plants were placed. Indeed, family Capparaceae and Cleomaceae are in clear need of more detailed systematic revision. Results Here, in the present study, the morphological characteristics and the ecological distribution as well as the genetic diversity analysis among the twelve species of both Capparaceae and Cleomaceae have been determined. The genetic analysis has been checked using 15 ISSR, 30 SRAP, and 18 ISTR to assess the systematic knots between the two families. In order to detect the molecular phylogeny, a comparative analysis of the three markers was performed based on the exposure of discriminating capacity, efficiency, and phylogenetic heatmap. Our results indicated that there is a morphological and ecological variation between the two families. Moreover, the molecular analysis confirmed that ISTR followed by SRAP markers has superior discriminating capacity for describing the genetic diversity and is able to simultaneously distinguish many polymorphic markers per reaction. Indeed, both the PCA and HCA data have drawn a successful annotation relationship in Capparaceae and Cleome species to evaluate whether the specific group sort individual or overlap groups. Conclusion The outcomes of the morphological and ecological characterization along with the genetic diversity indicated an insight solution thorny interspecies in Cleome and Gynandropsis genera as a distinct family (Cleomaceae) and the other genera (Capparis, Cadaba, Boscia, and Maerua) as Capparaceae. Finally, we recommended further studies to elucidate the systematic position of Dipterygium glaucum.


2020 ◽  
Vol 37 (1) ◽  
pp. 38-45
Author(s):  
O.A. Okukenu ◽  
A.A. Olajide ◽  
P.A. Dele ◽  
M. Wheto ◽  
B.T. Akinyemi ◽  
...  

This study was carried out to characterise Pennisetum purpureum harvested from some selected locations in S outh-W estern Nigeria using microsatellite markers. Leaf parts of growing young elephant grass (Pennisetum purpureum) were harvested and immediately preserved in ethanol solution before DNA extraction. Two (2) SSR primers (CTM59 and Xtxp278) were used to assess genetic diversity in Pennisetum purpureum. The result shows that 72% of the molecular variations in the elephant grass exists within the population with 28% among the population; there were no unique characteristics among the Nine (9) populations. Nei genetic index ranged from 0.067 (lowest) observed between Isokan and Odeda populations to 0.158 (highest), between Ifedore and Ikoyi Populations. Morphological characterization showed moderate diversity with two major clusters and one minor cluster. Keyword: Elephant grass; cultivars; locations; markers


Data in Brief ◽  
2018 ◽  
Vol 20 ◽  
pp. 101-107 ◽  
Author(s):  
Sheila A. Bishop ◽  
Hilary I. Okagbue ◽  
Olumuyiwa A. Oludayo ◽  
Olasunmbo O. Agboola ◽  
Michael C. Agarana ◽  
...  

2003 ◽  
Vol 54 (5) ◽  
pp. 429 ◽  
Author(s):  
J. S. Croser ◽  
F. Ahmad ◽  
H. J. Clarke ◽  
K. H. M. Siddique

Efforts to improve the yield and quality of cultivated chickpea (Cicer arietinum L.) are constrained by a low level of intraspecific genetic diversity. Increased genetic diversity can be achieved via the hybridisation of the cultivated species with the unimproved 'wild' relatives from within the 43 species of the Cicer genus. To date, the 8 species sharing an annual growth habit and chromosome number with C. arietinum have been the primary focus of screening and introgression efforts. Screening of these species has uncovered morphological characteristics and resistance to a number of abiotic and biotic stresses that are of potential value to chickpea improvement programs. Detailed analysis of protein and DNA, karyotyping, and crossability studies have begun to elucidate the relationships between the annual Cicer species. In comparison, perennial species have received little attention due to difficulties in collection, propagation, and evaluation. This review discusses the progress towards an understanding of genetic relationships between the Cicer species, and the introgression of genes from the wild Cicer species into the cultivated species.


2014 ◽  
Vol 67 (1) ◽  
pp. 125-137 ◽  
Author(s):  
Akbar Marzooghian ◽  
Mohammad Moghaddam ◽  
Mostafa Valizadeh ◽  
Mohammad Hasan Kooshki

AbstractEvaluation of the genetic diversity present within species is essential for conservation, management and utilization of the genetic resources. The objective of this study was to evaluate genetic variability of 70 common bean genotypes for seed storage proteins, grain morphological characteristics and agronomic traits. Two methods of extracting soluble seed proteins in salt were used.Positive correlations were observed among both seed morphological characters and developmental characters while yield components showed negative correlations with each other. Factor analysis for agronomic and grain morphological traits resulted in three factors were named yield components, seed morphology and phenology, respectively. Most genotypes had lower or medium scores for yield components and phenology factors. Considerable diversity was observed for seed morphology factor among the common bean genotypes.Nei’s diversity coefficient (He= 0.4), effective number of alleles (Ae= 1.69) and number of polymorphic loci (N = 17) indicated larger variation in the extraction method of soluble proteins in low salt (0.2 M NaCl) than high salt (1 M NaCl) condition. Considering that the centers of diversity for common bean are different in seed size, the result of Gst statistics showed that bands with relative mobility of 30, 32, 38 and 40 differentiated two weight groups more than other bands. Furthermore, significant differences were observed between these bands for number of pods per plant and number of seeds per plant.


2021 ◽  
Author(s):  
Carmen Julia Figueredo-Urbina ◽  
Gonzalo D. Álvarez-Ríos ◽  
Mario Adolfo García-Montes ◽  
Pablo Octavio-Aguilar

Abstract Background: The agaves are plants of cultural importance that have been used by humans for about 10,000 years for about 40 specific uses, of which the most culturally and economically important are fermented (Pulque) and distilled beverages (Mescal). Pulque continues to be produced in nearly all of Mexico, and the agaves used for this propose have shown domestication syndrome and selected morphological characteristics, such as larger plant size (since size is positively related to sap production) and less dentition of the leaves.Methods: In this study, we carry out an ethnobotanical, morphological and genetic analysis of the traditional varieties of pulque agave used in the production of aguamiel (agave sap) and pulque in two localities of the state of Hidalgo. We did semi-structured interviews and tours with crop owners, tlachiqueros (sap harvesters), and people related to the production system. We analyzed the morphology of 111 plants, and we studied the diversity and genetic structure of 130 individuals using 16 nuclear microsatellites.Results: We found wild, tolerated, transplanted, and cultivated varieties of agave. Asexual reproduction was the main method of propagation. We recorded 19 traditional varieties of pulque agave, 12 of them in production during the study, which correspond to the species Agave americana, A. salmiana y A. mapisaga with five intraspecific entities. The varieties were grouped according to a management gradient; the wild varieties were the smallest, with more lateral teeth and a larger terminal spine. The cultivated varieties clearly exhibited domestication syndrome, with larger plants and smaller dentition. The expected heterozygosity (He) of the varieties ranged between 0.204 to 0.721. Bayesian clustering suggested the existence of three genetic groups (K = 3), both at the level of traditional varieties of pulque agaves and for management categories, a result that matches multivariate clustering.Conclusions: Pulque producers in the studied localities, maintain high agrobiodiversity. The cultivated varieties exhibit domestication syndrome, as has been reported for other species of the genus with the same selection purposes. Our results support the hypothesis of a decrease in genetic diversity in crops compared to wild agaves, which seems to be due to vegetative propagation, among other factors.


Author(s):  
Obi Collins Uchechukwu ◽  
Aladeyelu Okikioluwa Stephen ◽  
Onyejike Darlington Nnamdi ◽  
Arusiwon Joseph Akinwale ◽  
Agbiogwu Ijeoma Nnenna

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