scholarly journals Uji Diagnostik Filariasis Menggunakan Rapid Diagnostic Test (RDT) Brugia malayi terhadap Pemeriksaan Mikroskopis di Desa Buntoi Kabupaten Gunung Mas, Provinsi Kalimantan Tengah

2021 ◽  
pp. 107-118
Author(s):  
Gadis Rinaty Susanty ◽  
Hernayanti Hernayanti ◽  
Dwi Sarwani Sri Rejeki
Keyword(s):  
Diagnostic Test ◽  
Rapid Diagnostic Test ◽  
Microscopic Examination ◽  
Predictive Value ◽  
Diagnostic Technique ◽  
Brugia Malayi ◽  
Diagnostic Study ◽  
Research Subjects ◽  
Cross Sectional ◽  
Central Kalimantan

Gunung Mas Regency, Central Kalimantan Province is one of the endemic filariasis areas with Microfilaria rate of 3.4%. One of the efforts made to control this problem is Mass Drug Administration once a year for 5 years. Currently, the Rapid Diagnostic Test (RDT) method is being developed, a quick and easy diagnostic technique to detect the presence of parasites in the patient's body. This study aims to determine the results of the filariasis diagnostic test using the Brugia malayi RDT on the microscopic examination in Buntoi Village, Gunung Mas Regency, Central Kalimantan Province. This research is a descriptive study with a cross-sectional approach with the research subjects all residents of Buntoi Village with inclusion and exclusion criteria totaling 161 samples. Collecting data was carried out by examination and interviews with questionnaires. Data analysis by calculating the microfilaria rate, sensitivity and specificity and calculating the frequency distribution of research variables. Data is presented in percentage form and displayed in tabular form. The results of the diagnostic study of B. malayi RDT and the microscopic examination were the same, i.e all were negative and no microfilariae were found. The diagnostic test for filariasis RDT Brugia malayi  on microscopic examination (SDJ) obtained 0% sensitivity, 100% specificity, 0% Positive Predictive Value and 100% Negative Predictive Value. The level of public knowledge about filariasis includes 61% good category, knowledge of MDA 40% good category and knowledge about prevention of filariasis in good category 53%.

scholarly journals Perbandingan Efektifitas Rapid Diagnostic Test (RDT) dengan Pemeriksaan Mikroskop pada Penderita Malaria Klinis di Puskesmus Mubune Kecamatan Likupang Barat

2018 ◽  
Vol 6 (2) ◽  
Author(s):  
Natanael Ritung ◽  
Victor D. Pijoh ◽  
Janno B. B. Bernadus
Keyword(s):  
Polymerase Chain Reaction ◽  
Diagnostic Test ◽  
Rapid Diagnostic Test ◽  
Microscopic Examination ◽  
Predictive Value ◽  
Public Health Problem ◽  
Malaria Diagnosis ◽  
Clinical Malaria ◽  
Chain Reaction ◽  
Polymerase Chain

Abstract: Malaria is still a public health problem worldwide, especially in economically underdeveloped and undeveloped countries. There are several laboratory diagnostic tests for malaria inter alia microscopic examination (thick and thin stained blood smear), rapid diagnostic test (RDT), and polymerase chain reaction (PCR). This study was aimed to compare the effectivity of RDT with of microscopic examination as the gold standard of malaria diagnosis. This was a diagnostic test study. Blood samples were obtained from 38 people of clinical malaria who lived at Likupang Barat from October 2015 to January 2016. The RDT results were compared with the microscopic examination to obtain the sensitivity and specifity levels. The results showed that of the RDT, the sensitivity was 67%, the specifity was 97%, the positive predictive value was 67%, and the negative predictive value was 97%. Conclusion: Rapid diagnostic test was nearly as effective as the microscopic examination of malaria.Keywords: RDT, microscopic examination, sensitivity, specificityAbstrak: Malaria masih menjadi masalah kesehatan di dunia terutama di negara yang secara ekonomis masih tertinggal dan belum berkembang. Diagnosis laboratorik malaria dapat dilakukan dengan beberapa cara antara lain pemeriksaan mikroskopik yaitu hapusan darah tebal dan hapusan darah tipis, rapid diagnostic test (RDT), dan polymerase chain reaction (PCR). Penelitian ini bertujuan untuk membandingkan tingkat efektifitas antara RDT dengan pemeriksaan mikroskopik yang merupakan baku emas diagnostik malaria. Jenis penelitian ialah uji diagnostik. Sampel darah diambil dari 38 orang dengan klinis malaria di Likupang Barat sejak Oktober 2015 - Januari 2016. Hasil pemeriksaan RDT dibandingkan dengan hasil pemeriksaan mikrsokopik untuk mengetahui tingkat sensivitas dan spesifisitasnya. Hasil penelitian mendapatkan tingkat sensivitas RDT secara umum sebesar 67%, spesifitas sebesar 97%, nilai duga positif sebesar 67%, dan nilai duga negatif sebesar 97%. Simpulan: Pemeriksaan RDT menunjukkan efektivitas dan akurasi yang hampir sama dengan pemeriksaan mikroskopik.Kata kunci: RDT, pemeriksaan mikroskopis, sensitivitas, spesifitas

scholarly journals Perbandingan Rapid Diagnostic Test dan Pemeriksaan Mikroskopik pada Diagnosis Malaria

2011 ◽  
Vol 5 (6) ◽  
pp. 250 ◽  
Author(s):  
Lambok Siahaan
Keyword(s):  
Positive Predictive Value ◽  
Negative Predictive Value ◽  
Diagnostic Test ◽  
Rapid Diagnostic Test ◽  
Parasite Density ◽  
Microscopic Examination ◽  
Predictive Value ◽  
Blood Film ◽  
Other Hand ◽  
Better Than

Penelitian ini dilakukan untuk membandingkan penggunaan Rapid diagnostic test dengan pemeriksaan mikroskopik (apusan darah) dalam menegakkan diagnosis malaria. Penelitian uji diagnostik ini dilakukan di Kabupaten Nias Selatan dan Kotamadya Sabang, Sumatera Utara. Apusan darah diwarnai dengan larutan Giemsa 10% dan dibaca oleh pemeriksa yang sudah terlatih. Rapid diagnostic test dilakukan dengan menggunakan parascreen. Uji diagnostik rapid diagnostic test secara umum diperoleh hasil yaitu sensitivitas 63,8%; spesifisitas 100%; Positive Predictive Value (PPV) 100%; dan negative predictive value (NPV)93,5%. Kepadatan parasit pada rapid diagnostic test (+) dan mikroskopik (+) adalah 1.483 ± 583 parasit/μl. Sementara, kepadatan parasit pada pemeriksaan rapid diagnostic test (-) dan mikroskopik (+) adalah 621 ± 335 parasit/μl. Pemeriksaan mikroskopik masih lebih baik dibandingkandengan rapid diagnostic test, terutama pada kepadatan parasit yang rendah. Kata kunci: Malaria, pemeriksaan mikroskopik, rapid diagnostic testAbstractThe study was conducted to compare rapid diagnostic test with microscopy (blood film) to diagnose malaria disease. This diagnostic test was done in South of Nias District and Sabang District, North Sumatera. Blood film were colored with Giemsa 10% solution and examined by trained microscopists. Rapid Diagnostic Test was done based on the manual procedure. The results of diagnostic test of rapid diagnostic test were sensitivity 63,5%; specificity 100%, positive predictive value (PPV) 100%, and negative predictive value (NPV) 93,5%. The density of parasite in rapid diagnostic test (+) were 1.483 + 583 parasite/μl (1.120-2.920 parasite/μl. In other hand, the density of parasite in rapid diagnostic test (-) and microscopy were 621 + 335 parasite/ μl (120-1.160 parasite/μl). Microscopy still better than rapid diagnostic test, especially in patient with low parasite density. Keywords: Malaria, microscopic examination, rapid diagnostic test

scholarly journals Perbandingan deteksi plasmodium spp. dengan cara pemeriksaan rapid diagnostic test dan pemeriksaan mikroskopik

2016 ◽  
Vol 4 (2) ◽  
Author(s):  
Nur M. Lasena ◽  
Victor D. Pijoh ◽  
Janno B. Bernadus
Keyword(s):  
Diagnostic Test ◽  
Rapid Diagnostic Test ◽  
Microscopic Examination ◽  
Predictive Value ◽  
Gold Standard ◽  
Detection Methods ◽  
Microscope Examination ◽  
Obligate Intracellular ◽  
Genus Plasmodium ◽  
Parasite Detection

Abstract: Malaria is caused by an obligate intracellular protozoa Plasmodium genus that may affect any people residing in its reproductive area. WHO has recommended quick parasite detection methods based on microscopic examination and Rapid Diagnostic Test (RDT) to all patients suspected of malaria before any antimalaria drug was prescribed. This study aimed to compare plasmodium spp. detection methods by using RDT and microscopic examination as the gold standard. The results of comparing RDT to microscopic examination showed 90.47% sensitivity, 100% specifity, 100% positive predictive value, and 87.5% negative predictive value. Conclusion: Rapid Diagnostic Test could detect malaria plasmodium almost the same as microscopic examination, and could be practically used as an alternative to confirm the diagnosis of malaria.Keywords: rapid diagnostic test, microscope examination, plasmodium sppAbstrak: Malaria merupakan penyakit yang disebabkan oleh protozoa obligat intrasel dari genus plasmodium yang dapat menyerang siapa saja terutama penduduk yang tinggal di daerah yang sesuai dengan kebutuhan perkembangan nyamuk. WHO merekomendasikan cara cepat mendeteksi parasit berdasarkan diagnosis dengan uji mikroskopik dan Rapid Diagnostic Test (RDT) pada semua pasien yang dicurigai malaria sebelum pengobatan antimalaria diberikan. Penelitian ini bertujuan untuk membandingkan deteksi plasmodium spp. dengan cara pemeriksaan RDT dan pemeriksaan mikroskopik yang merupakan gold standard. Hasil penelitian memperlihatkan bahwa pemeriksaan dengan metode RDT dibandingkan dengan metode mikroskopik menunjukkan sensitivitas 90,47%, spesifitas 100%, nilai prediksi positif 100%, dan nilai prediksi negatif 87,5%. Simpulan: Metode pemeriksaan Rapid Diagnostic Test dapat mendeteksi plasmodium malaria kurang lebih setara dengan pemeriksaan mikroskopik, dan dapat dijadikan pilihan (alternatif) untuk menetapkan diagnosis malaria secara praktis.Kata kunci: rapid diagnostik tes, pemeriksaan mikroskopik, plasmodium spp.

scholarly journals Blastocystis sp. Carriage and Irritable Bowel Syndrome: Is the Association Already Established?

Biology ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 340
Author(s):  
Fernando Salvador ◽  
Beatriz Lobo ◽  
Lidia Goterris ◽  
Carmen Alonso-Cotoner ◽  
Javier Santos ◽  
...  
Keyword(s):  
Irritable Bowel Syndrome ◽  
Microscopic Examination ◽  
Parasitic Infection ◽  
Diagnostic Technique ◽  
Control Group ◽  
Cross Sectional ◽  
Significant Difference ◽  
Blastocystis Sp ◽  
Irritable Bowel ◽  
Asymptomatic Subjects

Background: The aim of the present study is to describe the occurrence of Blastocystis sp. detection among asymptomatic subjects and patients with irritable bowel syndrome in order to evaluate the potential association between irritable bowel syndrome and the parasitic infection. Methods: Cross-sectional study where adult patients with irritable bowel syndrome diagnosed according to Rome IV criteria were included. A control group was formed by asymptomatic subjects older than 18 years. Exclusion criteria were: immunosuppressive condition or having received any drug with demonstrated activity against Blastocystis sp. within the last 6 months before study inclusion. Epidemiological and clinical information was collected from all included participants. Two stool samples were obtained from all participants: one sample for microscopic examination and one sample for Blastocystis sp. PCR detection. Blastocystis sp. infection was defined by the positivity of any of the diagnostic techniques. Results: Seventy-two participants were included (36 asymptomatic subjects and 36 patients with irritable bowel syndrome). Thirty-five (48.6%) were men, and median age of participants was 34 (IQR 29–49) years. The overall rate of Blastocystis sp. carriage was 27.8% (20/72). The prevalence assessed through microscopic examination was 22.2% (16/72), while the prevalence measured by PCR was 15.3% (11/72). When comparing the presence of Blastocystis sp. between asymptomatic subjects and IBS patients, we did not find any statistically significant difference (36.1% vs. 19.4% respectively, p = 0.114). Conclusions: regarding the occurrence of Blastocystis sp., no differences were found between asymptomatic participants and patients with irritable bowel disease irrespective of the diagnostic technique performed.

Streptococcal Pharyngitis Study

PEDIATRICS ◽  
1989 ◽  
Vol 83 (5) ◽  
pp. 808-808
Author(s):  
JENNIFER S. READ ◽  
ROBERT H. BEEKMAN
Keyword(s):  
Positive Predictive Value ◽  
Diagnostic Test ◽  
Rapid Diagnostic Test ◽  
Predictive Value ◽  
Bayes Theorem ◽  
Streptococcal Pharyngitis ◽  
Pediatric Setting ◽  
Group A

Redd and co-workers found the sensitivity of their rapid diagnostic test for group A streptococcal pharyngitis to be 62.8% and its specificity to be 96.9%. Furthermore, the positive predictive value of the test was determined to be 91.5%, sufficiently high to significantly influence the care provided to their patients. We strongly disagree with the authors' conclusion that their findings can be extrapolated to the general pediatric setting. Bayes theorem clearly relates a test's positive predictive value to its sensitivity as well as to the prevalence of true disease in the population.

scholarly journals Evaluation of a Rapid Diagnostic Test Currently Being Used in the Management of Malaria in Patients of the Hia/Chu-Parakou and the Boko Zone Hospital in Northern Benin

2021 ◽  
Vol 8 (4) ◽  
Author(s):  
Tokponnon F ◽  
◽  
Osse R ◽  
Houessou C ◽  
Akogbeto M ◽  
...  
Keyword(s):  
Diagnostic Test ◽  
Rapid Diagnostic Test ◽  
Probabilistic Method ◽  
Health Centers ◽  
Patient Identification ◽  
Cross Sectional ◽  
Predictive Values ◽  
Parasitological Diagnosis ◽  
Sensitivity Specificity ◽  
Adequate Management

Parasitological diagnosis is a fundamental element in the adequate management of the disease. In the last decade, there has been a resurgence of interest in the development of malaria Rapid Diagnostic Test (RDT) kits. This is the case with SD Bioline Malaria Ag Pf, which searches for HRP2 antigen by immunochromatography. The objective of this study is to compare the results of RDT SD Bioline Malaria Ag Pf in use with the results of Thick Gout (TG) in the biological diagnosis of malaria. This was a cross-sectional, descriptive and evaluative study carried out at the Hôpital d’Instruction des Armées-Center Hospitalier et Universitaire de Parakou and at the Boko zone hospital from April 20 to July 30, 2015. Patient identification, we used the non-probabilistic method and the convenience choice technique. The study involved 503 patients. The results of this study showed a good performance of the RDT SD Bioline Malaria Ag Pf. Among the 503 patients, 199 or 39.6% were positive for the RDT against 180 or 35.8% positive for the Thick Gout (TG). Sensitivity, specificity, positive and negative predictive values of the test compared to the thick film were respectively (91.7%, 89.5%, 82.9, 95) and the Kappa coefficient of 0.88 testifies a good match. False positive cases are noted in patients on treatment and even after recovery due to the persistence of the HRP2 antigen in the blood. However, it constitutes an interesting alternative to the management of malaria. At the end of this study, we suggest continuing the use of RDTs in health centers where microscopy is absent and/or reinforcing microscopy, and to strengthen staff training in the management of malaria cases.

scholarly journals Rapid Diagnostic Test Versus Microscopy for Diagnosing Malaria Among Pregnant Women in a Resource-Poor Setting; A Cross-Sectional Comparative Study

2020 ◽  
Vol 12 (8) ◽  
pp. 52
Author(s):  
Bartholomew N. Odio ◽  
Leonard O. Ajah ◽  
Perpetus C. Ibekwe ◽  
Monique I. Ajah ◽  
George O. Ugwu ◽  
...  
Keyword(s):  
Pregnant Women ◽  
Diagnostic Test ◽  
Rapid Diagnostic Test ◽  
Clinical Features ◽  
Scale Up ◽  
World Health ◽  
Resource Poor Setting ◽  
Cross Sectional ◽  
Significant Difference ◽  
Resource Poor

BACKGROUND: Diagnostic challenge of malaria in Nigeria remarkably impedes the World Health Organization (WHO) recommendation of laboratory diagnosis before treatment. Rapid Diagnostic Test (RDT) is easier and cheaper to perform when compared with microscopy especially in resource-poor settings. However there are conflicting results on the accuracy of RDT versus microscopy from previous studies. AIM: To compare the overall accuracy of   microscopy and RDT in detecting peripheral malaria among   pregnant women with clinical features of malaria. MATERIALS & METHODS: This was a cross-sectional comparative studyin whichRDT, microscopy and polymerase chain reaction (PCR) were performed using the peripheral bloodof the eligible study participants at the Alex Ekwueme Federal University Teaching Hospital, Abakaliki between September 1, 2016 and March 31, 2017.The PCR was used as the gold standard in this study. Data was analyzed with the Statistical Package for Social Sciences version 18 (IBM SPSS, Chicago, USA). P value ≤ 0.05 was considered statistically significant. RESULTS: The actual prevalent rates of malaria based on RDT, microscopy and PCR results among the participants were 58.2%, 59.9% and 61.1% respectively. There was no statistical significant difference among RDT, microscopy and combined RDT and microscopy on overall accuracy. Malaria infestation was associated with self-employed and unemployed women, primigravidity, second trimester, rural residence, non-use of long lasting insecticide treated nets and intermittent preventive therapy for malaria. CONCLUSION: There was no difference in overall accuracy among RDT, microscopy and combined RDT and microscopy. This underscores the need to scale up RDT for every patient with clinical features of malaria before treatment in this environment.

scholarly journals Effectivity of microscopic test as a simple diagnostic method to detect fat malabsorption in children

2019 ◽  
Vol 28 (4) ◽  
pp. 338-44
Author(s):  
Ariani Dewi Widodo ◽  
Muzal Kadim ◽  
Ina Susianti Timan ◽  
Nuraini Irma Susanti ◽  
Fatima Safira Alatas ◽  
...  
Keyword(s):  
Predictive Value ◽  
Gold Standard ◽  
Diagnostic Study ◽  
Normal Children ◽  
Cross Sectional ◽  
Primary Endpoints ◽  
Sudan Iii ◽  
Fat Malabsorption ◽  
Moderate Sensitivity ◽  
Sensitivity Specificity

BACKGROUND Lipid malabsorption causes many health problems, for example stunting, a major worldwide issue. There has not been any assessment on the effectivity of lipid microscopic test in diagnosing lipid malabsorption. This research was aimed to study the effectivity of lipid microscopic test in detecting lipid malabsorption in children. METHODS This was a cross-sectional diagnostic study that evaluated the effectivity of lipid microscopic test using Sudan III against steatocrit test as the gold standard in diagnosing lipid malabsorption. The study was done in 68 children aged 6–60 months in Cipto Mangunkusumo Hospital, Jakarta. Results of lipid microscopic test were compared with that of steatocrit test among children with lipid malabsorption and normal children. The primary endpoints of this study are the sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV). RESULTS A total of 68 children consisting of 41 boys and 27 girls were included, with a median age of 14.3 months. The most frequently found stool consistency was mushy (50%). The most common result of microscopic test, found in 42% of subjects, was positive 1. Sensitivity, specificity, PPV, and NPV of lipid microscopic test were 49.15%, 66.67%, 90.63%, and 16.67%, respectively. CONCLUSIONS Lipid microscopic test has a moderate sensitivity in diagnosing fat malabsorption and needs to be complemented with other methods such as steatocrit.

scholarly journals Advantage of ultrasonography in the diagnosis of acute appendicitis at Al-Karama Teaching Hospital

2019 ◽  
Vol 6 (3) ◽  
pp. 702
Author(s):  
Mohammed Hillu Surriah ◽  
Amine Mohammed Bakkour ◽  
Nidaa Ali Abdul Hussain
Keyword(s):  
Acute Appendicitis ◽  
Teaching Hospital ◽  
Predictive Value ◽  
Diagnostic Technique ◽  
Cost Effective ◽  
Cross Sectional Study ◽  
Negative Appendectomy ◽  
Cross Sectional ◽  
Suspected Appendicitis ◽  
Presenting Symptoms

Background: The clinical diagnosis of acute appendicitis remains a challenge to surgeons. Different aids were introduced to improve the diagnostic accuracy. Among these modalities, ultrasonography is simple, easily available, non-invasive, convenient and cost effective. The aim of the study was to determine the validity of ultrasound in diagnosis of the acute appendicitis in those with clinically diagnosed patients.Methods: A cross sectional study was carried out in Al-Karama teaching hospital for thirty months from the period of 1st June 2016 to 1st December 2018. All patients with suspected appendicitis underwent clinical evaluation then sent for US. Results of surgeries, where relevant, were compared against US results. Positive and negative appendices on histopathology were regarded in accordance to the criteria which was negative appendectomy was defined as normal looking appendix and absence of acute inflammation on histopathology while positive cases included appendices showing acute inflammatory changes. Sensitivity, specificity and overall accuracy was calculated.Results: A total of 435 patients with suspected appendicitis, males 224 (51.49%) and females 211 (48.50%) were included in present study. There were no significant differences between patients with positive and negative histopathology findings regarding presenting symptoms. There was a significant association between (cough sign, localized tenderness sign and pointing sign) and patients with positive histopathology findings. Regarding to the validity results of ultrasound in comparison to histopathology findings were  accuracy 87.6%, sensitivity 87.8%, specificity 85.3%, positive predictive value 98.6% and negative predictive value 62.8%.Conclusions: The ultrasonography had a good accuracy, sensitivity and specificity in diagnosing acute appendicitis cases. Negative with ultrasonography results should be re-examined with different diagnostic technique like CT-scan.

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