scholarly journals Alpha-glucosidase inhibitory and antioxidant activities of various extracts of aerial parts of Fagonia indica Burm. F.

2021 ◽  
Vol 18 (4) ◽  
pp. 791-797
Author(s):  
Atiq-ur-Rehman ◽  
Abida Latif ◽  
Nasir Abbas ◽  
Imran Waheed ◽  
Atta-ur-Rehman ◽  
...  
Keyword(s):  
Antioxidant Activities ◽  
Scientific Evidence ◽  
Radical Scavenging ◽  
Chloroform Extract ◽  
Traditional Use ◽  
Aerial Parts ◽  
Ic50 Value ◽  
Treatment Of Diabetes ◽  
Alpha Glucosidase

Purpose: To investigate in vitro antioxidant and anti-diabetic potentials of Fagonia indica Burm.f. Var. indica in order to provide scientific evidence for its traditional use. Methods: Cold maceration method was employed for the preparation of extracts of aerial parts of the plant using chloroform, n-hexane, methanol and water as solvents. Total flavonoid and polyphenolic content of various extracts were determined by standard methods. The antioxidant and anti-diabetic activities of the extracts were determined using 1, 1-diphenyl-2-picrylhydrazyl (DPPH) and in vitro αglucosidase (maltase) inhibitory assays, respectively. Results: Preliminary results indicated the presence of various phytochemicals in the extracts. The chloroform extract exhibited the highest contents of total flavonoids (65.98 ± 1.63 mg QE/g) and polyphenols (26.75 ± 1.09 mg GAE/g). This extract also showed the highest free radical scavenging (64.74 ± 1.43 %) with IC50 value of 34.18 ± 5.57 µg/mL while the methanol extract exerted the highest αglucosidase (maltase) inhibitory activity (45.22 ± 0.46 %) with half-maximal concentration (IC50) of 220.4 ± 0.41 µg/mL. Conclusion: The extracts of the aerial parts of Fagonia indica possess significant anti-diabetic and antioxidant effect, thus justifying the traditional use of the plant for treatment of diabetes.

scholarly journals GC-MS Chemical Constituents and in vitro Activities of the Oil Extract of Euryops brevipapposus Leaves used Traditionally for the Management of Some Diseases

2021 ◽  
Vol 33 (3) ◽  
pp. 677-685
Author(s):  
Abiodun Atinuke Adegborioye ◽  
Oyinlola Oluwunmi Olaokun ◽  
Benson Chuks Iweriebor ◽  
Larry Chikwulu Obi
Keyword(s):  
Local Community ◽  
Chemical Constituents ◽  
Scientific Evidence ◽  
Radical Scavenging ◽  
Traditional Use ◽  
Ic50 Value ◽  
Oil Extract ◽  
Radical Scavenging Ability ◽  
Vibrio Spp

Euryops brevipapposus (Asteraceae) is a medicinal plant of a local community utilized traditionally for its recognized effectiveness in managing non-communicable diseases, especially asthma. The traditional use of E. brevipapposus lacks scientific evidence and the increased burden of asthma makes confirming this claim paramount. The study characterized by GC-MS the bioactive compounds of E. brevipapposus essential oil (EbO) extracted with Clevenger apparatus. The antibacterial efficacy and antioxidant activity by free radical scavenging ability were investigated in vitro using standard methods. A strong antioxidant IC50 value of 6.71 × 10-7 mg/mL of oil was obtained for DPPH. The antibacterial activity against Escherichia coli and Vibrio spp. (MIC value of 0.055 mg/mL) was superior. GC-MS analysis of EbO showed α-phellandrene, α-pinene, germacrene D, β-pinene, β-mycrene, (E)-β-ocimene and bicyclogermacrene as the major compounds. The antioxidant and antibacterial potentials of E. brevipapposus may justify the therapeutic claims and local usage of this plant.

scholarly journals Phytochemistry and Biological Activities of Iris Species Growing in Iraqi Kurdistan and Phenolic Constituents of the Traditional Plant Iris postii

Molecules ◽  
2021 ◽  
Vol 26 (2) ◽  
pp. 264
Author(s):  
Hawraz Ibrahim M. Amin ◽  
Faiq H. S. Hussain ◽  
Soran K. Najmaldin ◽  
Zaw Min Thu ◽  
Mohammed Farhad Ibrahim ◽  
...  
Keyword(s):  
Antioxidant Activities ◽  
Biological Activities ◽  
Radical Scavenging ◽  
Chromatographic Separations ◽  
Traditional Use ◽  
Methanolic Extracts ◽  
Aerial Parts ◽  
Traditional Remedies ◽  
Phenolic Constituents ◽  
Total Antioxidant

A dozen Iris species (Iridaceae) are considered traditional remedies in Kurdistan, especially for treating inflammations. Phytochemical studies are still scarce. The information reported in the literature about Iris species growing in Kurdistan has been summarized in the first part of this paper, although, except for Iris persica, investigations have been performed on vegetal samples collected in countries different from Kurdistan. In the second part of the work, we have investigated, for the first time, the contents of the methanolic extracts of Iris postii aerial parts and rhizomes that were collected in Kurdistan. Both extracts exhibited a significant dose-dependent free radical scavenging and total antioxidant activities, comparable to those of ascorbic acid. Medium-pressure liquid chromatographic separations of the two extracts afforded l-tryptophan, androsin, isovitexin, swertisin, and 2″-O-α-l-rhamnopyranosyl swertisin from the aerial parts, whereas ε-viniferin, trans-resveratrol 3,4′-O-di-β-d-glucopyranoside, and isotectorigenin were isolated from the rhizomes. This is the first finding of the last three metabolites from an Iris species. The various remarkable biological activities of isolated compounds scientifically sustain the traditional use of I. postii as a medicinal plant.

scholarly journals Bioactivities of Water Extract and Essential Oil from the Mace of Myristica fragrans Houtt

2018 ◽  
Vol 26 (2) ◽  
pp. 099
Author(s):  
Adit Widodo Santoso ◽  
Adelina Simamora ◽  
Adelina Simamora ◽  
Kris Herawan Timotius ◽  
Kris Herawan Timotius
Keyword(s):  
Essential Oil ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Water Extract ◽  
Antibacterial Activities ◽  
Diffusion Method ◽  
Total Phenolic ◽  
Traditional Use ◽  
Myristica Fragrans

<p><em>Myristica fragrans</em> Houtt (nutmeg) is used as a spice and flavour for food and beverages. It has been traditionally used to treat a number of medical conditions, including diabetes mellitus. The study was undertaken to scientifically validate the traditional use of mace from <em>M.</em><em> </em><em>Fragrans</em><strong>. </strong>The objectives of this study were to evaluate α-glucosidase inhibition, antioxidant and antibacterial activities of water extract (WE) and essential oil (EO) from <em>M.</em><em> </em><em>fragrans</em> mace.  Both WE and EO were evaluated for their α-glucosidase inhibitory activities <em>in vitro</em> and their antioxidant activities based on DPPH radical scavenging assay. Standard compounds were used for every test. Total phenolic and flavonoid contents of both extracts were also determined. The extracts were also tested for their antibacterial activities against six different bacteria by a well diffusion method. Both extracts showed inhibition activities against α-glucosidase, with WE showed stronger activity than EO (IC<sub>50</sub> = 1.86 and 8.15 mg/ml). Good radical scavenging activities were observed for both extracts, with WE showed stronger activity than EO (IC<sub>50</sub> = 1.51 and 4.59 mg/ml). WE showed higher content in phenolic than EO (47.84 and 37.21 mg GAE/100 g DW). Flavonoid content in WE was also higher than EO (215.36 and 30.12 mg RE/ml). Based on the well diffusion method, only EO exhibited antibacterial activities, with inhibition zone in the range 1.03 – 1.30 mm.  The strongest activity was observed against<em> Staphylococcus mutans</em>. The results indicate WE and EO can be exploited further for pharmacological uses, in particular for their antidiabetic and antioxidant activities. </p>

scholarly journals In-Vitro Antioxidant Activity and Total Phenolic Content of Ruta montana L. Extracts

2020 ◽  
Vol 10 (2) ◽  
pp. 69-75
Author(s):  
Mounira Merghem ◽  
Saliha Dahamna
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Chloroform Extract ◽  
Total Phenolic ◽  
Ruta Montana ◽  
Β Carotene

The aim of this study is to evaluate in vitro antioxidant activities of Ruta montana L.  extracts. This activity was evaluated by three methods : DPPH (2, 2'-diphenyl- 1- picrylhydrazy), bleaching of β-carotene and chelation of ferrous iron. Results showed that ethyl acetate extract (EAE) represents the highest amount of total polyphenols, tannins and flavonoids with 257,1 ± 0,703µg gallic acid equivalent/mg of extract,  251 ± 1.41 µg tannic acid equivalent /mg of extract,117,4 ± 3,451 µg quercetin equivalents/mg of extract, 139,5 ± 4,107 µg rutin equivalents/mg of extract, respectively. In the DPPH assay, ethyl acetate extract showed the higher scavenging capacity (IC50 = 0.044 ± 0.001 mg/ml) followed by methanol, aqueous and chloroform extract. Whereas, AqE showed the best chelating effect and the best inhibitory capacity of the coupled oxidation of linoleic acid/ β-carotene. Keywords: Ruta montana L; polyphenols; antioxidant activity; free radical scavenging.

Phytoecdysteroids of Silene guntensis and their in vitro Cytotoxic and Antioxidant Activity

2011 ◽  
Vol 66 (5-6) ◽  
pp. 215-224 ◽  
Author(s):  
Nilufar Z. Mamadalieva ◽  
Mahmoud Z. El-Readi ◽  
Abdulaziz A. Janibekov ◽  
Ahmad Tahrani ◽  
Michael Wink
Keyword(s):  
Antioxidant Activities ◽  
Chloroform Extract ◽  
Breast Adenocarcinoma ◽  
Cytotoxic Effects ◽  
Chemical Structures ◽  
Aerial Parts ◽  
Human Cervix ◽  
Mcf 7 ◽  
C Nmr

Phytoecdysteroids from aerial parts of Silene guntensis B. Fedtsch were investigated and three phytoecdysteroids were isolated: 2,3-diacetate-22-benzoate-20-hydroxyecdysone (1), 2-deoxy-20-hydroxyecdysone (2), and 20-hydroxyecdysone (3). Their chemical structures were elucidated by DEPT, COSY, 1H and 13C NMR spectroscopy. The isolated compounds 1 - 3 and crude extracts were evaluated for their antiproliferative and antioxidant activities. They exhibited substantial inhibition of cell growth against human cervix adenocarcinoma (HeLa), hepatocellular carcinoma (HepG-2), and breast adenocarcinoma (MCF-7) cells. The chloroform extract showed potent cytotoxic effects [IC50 values (26.58 ± 1.88) μg/mL, (20.99 ± 1.64) μg/mL, and (18.89 ± 2.36) μg/mL, respectively]. The new compound 1 was mildly cytotoxic compared to extracts [(127.97 ± 11.34), (106.76 ± 7.81), and (203.10 ± 19.56) μg/mL, respectively]. Water and n-butanol extracts exhibited good antioxidant activities [IC50 values of (68.90 ± 6.45) μg/mL and (69.12 ± 5.85) μg/mL, respectively].

scholarly journals Estimation of Cholinesterase Inhibitory and Antioxidant Effects of the Leaf Extracts of Anatolian Ficus carica var. domestica and their Total Phenol and Flavonoid Contents

2011 ◽  
Vol 6 (3) ◽  
pp. 1934578X1100600 ◽  
Author(s):  
Ilkay Erdogan Orhan ◽  
Osman Üstün ◽  
Bilge Şener
Keyword(s):  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Chloroform Extract ◽  
Total Phenol ◽  
Ficus Carica ◽  
Cholinesterase Inhibition ◽  
Leaf Extracts ◽  
Antioxidant Power

Ficus carica var. domestica Tsch. & Rav. (common fig) is widely grown in Turkey and exported for its edible fruits. In this study, the n-hexane, chloroform, acetone, methanol, n-butanol, and water extracts of the leaves of F. carica var. domestica were screened for their cholinesterase inhibitory and antioxidant activities. Cholinesterase inhibition against acetyl- (AChE) and butyrylcholinesterase (BChE) was measured by the spectrophotometric method of Ellman at concentrations of 25, 50, and 100 μg/mL., while antioxidant activity was tested using three in vitro methods; 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, metal-chelation capacity, and ferric-reducing antioxidant power (FRAP). Total phenol and flavonoid contents of the extracts were determined spectrophotometrically. Our results revealed that the n-hexane and acetone extracts exerted a notable inhibition against both AChE (62.9±0.9% and 50.8±2.1%, respectively) and BChE (76.9±2.2% and 45.6±1.3%, respectively). However, they had low activity in the antioxidant tests. The chloroform extract was found to be the richest in total flavonoid content (252.5±1.1 mg/g quercetin equivalent), while the n-butanol extract had the highest total phenol amount (85.9±3.2 mg/g extract gallic acid equivalent).

scholarly journals Chemical Profile and In Vitro Bioactivity of Vicia faba Beans and Pods

Proceedings ◽  
2020 ◽  
Vol 70 (1) ◽  
pp. 45
Author(s):  
Monica Rosa Loizzo ◽  
Marco Bonesi ◽  
Mariarosaria Leporini ◽  
Tiziana Falco ◽  
Vincenzo Sicari ◽  
...  
Keyword(s):  
Vicia Faba ◽  
Faba Bean ◽  
Radical Scavenging ◽  
Human Consumption ◽  
Potential Health ◽  
Antioxidant Power ◽  
Ic50 Value ◽  
Faba Beans ◽  
Alpha Glucosidase

Vicia faba L. (faba bean) (Fabaceae) is cultivated worldwide as a crop for human consumption. In this study, beans and pods were investigated for their phytochemical content and their potential nutraceutical properties as a strategy to counteract metabolic syndrome (MetS). Pods represent a faba bean industrial processing by-product. Pod ethanol extract showed the highest total phenol and flavonoid content. High Performance Liquid Chromatography (HPLC) analysis revealed that in both pods and bean, (+)-catechin and (−)-epicatechin were the two most abundant compounds. 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic) acid (ABTS), Ferric Reducing Antioxidant Power (FRAP), β-carotene bleaching were used to test V. faba antioxidant activity. The inhibition of alpha-amylase, alpha-glucosidase, and lipase was studied. Pod extract showed an ABTS radical scavenging ability (IC50 value of 1.5 mg/mL) comparable to ascorbic acid (IC50 value of 1.7 mg/mL) used as a positive control, whereas bean extract was the most active in protecting lipid peroxidation. A promising alpha-glucosidase inhibitory activity was also observed with the edible portion of faba beans (IC50 value of 38.31 mg/mL). Collectively, our results demonstrated the potential health properties of V. faba edible and inedible portions.

scholarly journals STUDY ON ANTIOXIDANT ACTIVITIES OF THE AERIAL PARTS AND SOME COMPOUNDS ISOLATED FROM Archidendron clypearia ((Jack) I. Niels Part 2. ISOLATING, DETERMINING STRUCTURE AND ANTIOXIDANT CAPABILITY OF SOME COMPOUNDS FROM ETHYL ACETATE EXTRACT

2016 ◽  
Vol 54 (4) ◽  
pp. 452
Author(s):  
Le Trung Hieu ◽  
Tran Thi Van Thi ◽  
Nguyen Thi Hoai ◽  
Vo Thi Mai Huong
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
2D Nmr ◽  
Chloroform Extract ◽  
In Vitro Tests ◽  
Methyl Gallate ◽  
Aerial Parts ◽  
Isolated Liver Cells ◽  
High Antioxidant Activity

The antioxidant activity in vitro of methanol extract of Archidendron clypearia was evaluated by in vitro tests on isolated liver cells of mouses with ED50 value ​​ of 2.18 μg/mL compared to that of curcumin of 1.87 µg/mL. Using combined chromatographic methods, four compounds were isolated from chloroform extract of the Archidendron clypearia. Their structures were elucidated to be daucosterol, 1-octacosanol, docosenoic acid, and methyl gallate by 1D- and 2D-NMR spectroscopic methods and in comparison with those reported in the literature. This is the first report of these compounds from the plant. All four compounds showed quite high antioxidant activity, for which methyl gallate was the highest one.

scholarly journals Synthesis, Antioxidant, Anticholinesterase Activities and Molecular Docking Studies of coumaryl 1,3-selenazoles Derivatives

2020 ◽  
Author(s):  
Nurul Zawani Alias ◽  
Muhd Hanis Md Idris ◽  
Nurhafizoh Abdul Somat ◽  
Norwaziah Mahmud ◽  
Sharizal Hasan ◽  
...  
Keyword(s):  
Active Site ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Cation Radical ◽  
Docking Studies ◽  
Potent Inhibition ◽  
Ic50 Value ◽  
Acetylcholine Level ◽  
Radical Scavenging Assay

Inhibition of acetylcholinesterase (AChE) enzyme is a known procedure to treat severe Alzheimer's disease through increasing the acetylcholine level in the brain and thus slowing down the progression of Alzheimer's symptoms. The approved medications are only considered as palliative and addressed some reported deficiencies. Therefore, the demand for safe and effective compounds is substantially increasing. A newly series of coumaryl 1,3-selenazoles derivatives was synthesized in four steps. Then, their antioxidant activities were evaluated using DPPH, ABTS cation radical scavenging assay and cupric reducing antioxidant capacities (CUPRAC). The anticholinesterase activities were evaluated using the Ellman method. Then, the docking studies were carried out to explain the possible correlation between in vitro anticholinesterase activity results and the ligand-receptor interactions. Ten new coumaryl 1,3-selenazoles (5a-5d series and 6a-6f series) derivatives were successfully synthesized. The DPPH radical scavenging assay showed that all tested compounds have IC50 value &amp;gt; 200 &mu;M, for ABTS cation radical scavenging assay the IC50 value &amp;gt; 1000 &mu;M and for CUPRAC assay the IC50 value &amp;gt; 200 &mu;M. Compound 5c was found to be the most active compound against AChE and BChE in its series with IC50 value for AChE is 99.76 &mu;M and IC50 for BChE is 140.28 &mu;M while 6b exhibited the most potent inhibition in its series with IC50 value for AChE is 56.01 &mu;M and IC50 for BChE is 121.34 &mu;M. Besides, the docking studies showed that compound 5c and 6b formed &pi;-&pi; stacking interaction with aromatic residues at the active site of AChE and BChE, which is responsible for inhibiting the enzymes. This shows that the synthesized compounds contain skeletal structures that can interact and inhibit within the enzymes active site.

scholarly journals In vitro antioxidant and anti-inflammatory activities of the chloroform extract of Combretum punctatum var squamosum

2019 ◽  
Vol 19 (3) ◽  
pp. 100-108
Author(s):  
K. Thanzami ◽  
B.B. Kakoti ◽  
C. Lalremruati
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Chloroform Extract ◽  
In Vitro Models ◽  
Percent Inhibition ◽  
Anti Inflammatory

The objective of this study was to investigate the antioxidant and anti-inflammatory activities of the chloroform extract of Combretum punctatum var squamosum by in vitro models. Antioxidant activity was determined by 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, reducing power and lipid peroxidation assays. The phenolic and flavonoid contents of the extract were also assessed. The extract at different concentrations showed significant percent inhibition of DPPH and lipid peroxidation and high reducing power when compared with the standard. The extract also showed a significantly high content of phenolics and flavonoids. Anti-inflammatory activity was studied by inhibition of albumin denaturation and human red blood cell (HRBC) membrane stabilization methods. The extract at different concentrations showed significant percent inhibition of albumin denaturation and percent inhibition of haemolysis when compared with the standard. Thus, the result indicates that the chloroform extract exhibited significant potential on anti-inflammatory and antioxidant activities.

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