scholarly journals Mir-23b down-regulates the expression of target gene of acetaldehyde dehydrogenase 1a1 and increases the sensitivity of cervical cancer stem cells to cisplatin

2021 ◽  
Vol 20 (1) ◽  
pp. 29-34
Author(s):  
Yongbing Tao ◽  
Fuyun Mao ◽  
Weihong Gu ◽  
Ling Wu ◽  
Jing Guo ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Stem Cells ◽  
Cervical Carcinoma ◽  
Hela Cells ◽  
Target Gene ◽  
Control Group ◽  
Cervical Cancer Cell Line ◽  
Acetaldehyde Dehydrogenase ◽  
Cell Line Hela ◽  
Inhibition Group

Purpose: To study the effect of miR-23b on the expression of the target gene of acetaldehyde dehydrogenase 1A1 (ALDH1A1), and cisplatin (CDDP) susceptibility of cervical carcinoma stem cells. Methods: Human cervical cancer cell line Hela cells were cultured in vitro, and miR-23b mimic and negative control were transfected into the cells using lipofectamine method. The growth of the two groups of cells was determined using growth curve method, and their proliferation measured using plate clone formation. The influence of treatments on the sensitivity of the cells to CDDP was assayed using MTT method. The mRNA expression of ALDH1A1 in Hela cells was assayed using real-time quantitative polymerase hain reation (PCR), while its protein expression was assayed by Western blot. Results: The levels of expressions of ALDH1A1 protein and mRNA in the miR-23b overexpression group were significantly lower than those in the control group (p < 0.05). The sensitivities of Hela cells to CDDP in the ALDH1A1 inhibition group and the control group were dose-dependent to some extent, but cell inhibition in ALDH1A1 inhibition group markedly increased, relative to control when the CDDP dose was 0.1 ppc (p < 0.01). Conclusion: Up-regulating the expression of miR-23b significantly inhibits the growth and proliferation of cervical cancer cells, and increases their sensitivity to CDDP via down-regulation of the expression of the target gene for ALDH1A1. Therefore, during cervical carcinoma treatment, increasing the level of miR-23b may produce a chemotherapeutic effect. Keywords: MiR-23b, Acetaldehyde dehydrogenase 1A1, Cervical cancer, Cisplatin, Sensitivity

The regulatory mechanism involved in paclitaxel-induced apoptosis: The role of ATF-3 on apoptosis in cervical cancer cell line (HeLa cell)

2007 ◽  
Vol 25 (18_suppl) ◽  
pp. 16052-16052
Author(s):  
Y. Kwon
Keyword(s):  
Cervical Cancer ◽  
Hela Cells ◽  
Cervical Cancer Patient ◽  
Cervical Cancer Cell Line ◽  
Similar Data ◽  
Functional Link ◽  
Transfected Cells ◽  
Cell Line Hela ◽  
Induced Apoptosis ◽  
Atf3 Expression

16052 Background: Paclitaxel, which is a potent anti-cancer drug and microtubule-binding agent, has been found to be effective against several tumors, including cervical cancer. However, the exact mechanism underlying the apoptotic effects of paclitaxel is poorly understood. Methods: We show that paclitaxel induces apoptosis, as demonstrated by DNA fragmentation, TUNEL assay, and flow cytometry analysis in cervical cancer HeLa cells, correlating with enhanced caspase-3 activation and p73 as well as p21, which are inhibited strongly by p73 siRNA. Results: The paclitaxel treatment followed by the overexpression of wild type ATF3 potentiated apoptosis is by enhancing p73 expression, but these events were attenuated in C-terminal deleted ATF3 or ATF3 siRNA-transfected cells. Interestingly, paclitaxel-induced ATF3 colocalizes with transfected GFP-p73 in nucleus, but not in p73 siRNA-transfected cells. Conversely, p73 may not affect ATF3 expression, as increase of ATF3 expression did not detect in p73-overexpressing cells. Furthermore, we observed that ATF3 binds to DNA binding domain of p73, confirmed by GST-pull down assay for ATF3. Additionally, ATF3 potentiates p21 gene, a downstream target of p73, promoter activity and p73-p21 binding activity induced by paclitaxel and also we have obtained similar data in the tissue of cervical cancer patient treated with paclitaxel. Conclusions: Collectively, these results demonstrate that overexpression of ATF3 potentiates paclitaxel-induced apoptosis in HeLa cells, at least in part, through stimulating the p73 expression and its transcriptional activity. ATF3 may function as a tumor-inhibiting factor by regulating directly p73 and may suggest a functional link between ATF3 and p73. No significant financial relationships to disclose.

scholarly journals The Anti-Cancer Property of Mumie as Natural Product on Human Cervical Cancer Cell Line (HeLa)

2020 ◽  
Vol 9 (1) ◽  
pp. 196-202
Keyword(s):  
Cervical Cancer ◽  
Hela Cells ◽  
Chromatin Condensation ◽  
Cancer Cell Line ◽  
Antioxidant Property ◽  
Cervical Cancer Cell Line ◽  
Cell Line Hela ◽  
Anti Cancer ◽  
Human Cervical Cancer Cell ◽  
Treated Culture

Mumie is a natural component found in some mountains, such as the Himalayas, as well as in some mountainous of Iran. It contains of humic and phenolic compounds that have antioxidant and anti-cancer properties. Therefore, in this study, anti-cancer and antioxidant properties of mumie were examined on Human Cervical Cancer Cell Line (HeLa). HeLa cells and normal fibroblasts (NIH) were cultured in DMEM/F12 with mumie at concentrations of 0, 100, 200, 300, 400, 500 and 1000 µg/ml for 24 and 48 h. The bioviability of these cells were evaluated using MTT assay. Chromatin condensation and apoptosis of these cells were examined using acridine orange and aniline blue staining respectively. Antioxidant property of mumie on NIH cells was evaluated by 10 mM H2O2 and neutral red test. MTT assay revealed bioviability of HeLa cells decreased but chromatin condensation increased in concentration of 100 μg/ml mumie treated culture. Apoptosis of the HeLa cells were observed in 100 μg/ml mumie treated culture. Mumie did not affect the bioviability, chromatin condensation and apoptosis of NIH cells but 500 and 1000 μg/ml concentrations were toxic and induced cell death. The cell cultures in different concentrations of mumie after 24and 48 h showed the similar results. NIH cells bioviability increased in 500 and 1000 μg/ml concentrations of co-culture of H2O2 and mumie that confirmed the antioxidant property. It concluded that even low concentrations of mumie could destroy HeLa cells without any side effect on normal cells. Therefore, it can be used for cervical cancer treatment but further research is needed.

scholarly journals CYTOTOXICITY AND APOPTOSIS INDUCTION BY KAFFIR LIME LEAVES EXTRACT (Citrus hystrix DC.) IN HeLa CELLS CULTURE (HUMAN CERVICAL CANCER CELL LINE)

2015 ◽  
Vol 2 (1) ◽  
pp. 631 ◽  
Author(s):  
Nastiti Wijayanti ◽  
W.A.S. Tunjung ◽  
Y. Setyawati
Keyword(s):  
Cervical Cancer ◽  
Ethyl Acetate ◽  
Cell Line ◽  
Cancer Cell ◽  
Hela Cells ◽  
Ethyl Acetate Extract ◽  
Cancer Cell Line ◽  
Cervical Cancer Cell ◽  
Cervical Cancer Cell Line ◽  
Cell Line Hela

<p>Kaffir lime (Citrus hystrix) is a native plant of Indonesia. Nowadays the use of plants is limited as ingredients in Indonesia cuisine and aromatherapy oils for industry. Leaves of C. hystrix contain of polyphenols and essential oils thus they were possibly cytotoxic to cancer cell line. The objective of this research was to determine the cytotoxicity effect and apoptosis induced by leaves extract C. hystrix to cervical cancer cell line (HeLa cells). Methods used in this study included sampling of kaffir lime leaves, extractionusing three different solvents (ethanol, ethyl acetate, and hexane), detection of metabolite secondary compound (alkaloids, flavonoids, terpenoids, saponins, and tannins) using thin layer chromatography (TLC), cytotoxicity assay via MTT assay, and apoptosis test with double staining method (ethidium bromide-acrydine orange). Result showed kaffir lime crude extract dose dependently inhibitHeLa cells proliferation. lC50of ethanolicand ethyl acetate extract was 82,034 µg/mL and 57,845 µg/mL, respectively means cytotoxic to HeLa cells. On the other hand lC50 of hexane extract was 203,992 µg/mL which was not cytotoxic. Furthermore ethanolic and ethyl acetate extract were able to induce apoptosis of HeLa cells by increasing the number of apoptotic cells. In conclusion, kaffir lime leaves extract had cytotoxic effect and induced apoptosis. Moreover ethyl acetate extractof kaffir lime was the most potential to induce apoptosis in HeLa cells. </p><p><strong>Keywords</strong>: kaffir lime leaves (Citrus hystrix), HeLa, MTT, cytotoxicity, apoptosis.</p>

scholarly journals PIM-1 may function as an oncogene in cervical cancer via activating the EGFR signaling

2020 ◽  
Vol 35 (3) ◽  
pp. 67-73 ◽  
Author(s):  
Hongwen Yang ◽  
Kui He ◽  
Weile Dong ◽  
Jinchuan Fang ◽  
Suyun Zhong ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Hela Cells ◽  
Cancer Cell Line ◽  
Annexin V ◽  
Cervical Cancer Cell Line ◽  
Cancer Tissue ◽  
Egfr Signaling ◽  
Adjacent Tissue ◽  
Cervical Cancer Tissue ◽  
Cell Line Hela

Background: This work was designed to explore the roles of PIM-1 in the development of cervical cancer. Methods: There were 90 paired cervical tumor samples and the non-tumor adjacent tissue. The levels of PIM-1 in different samples were examined using quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR) methods. The potential diagnostic value of PIM-1 was analyzed by the receiver operating characteristic (ROC) curve; furthermore, the expression of EGFR in tumor samples was detected, and Pearson’s correlation analysis was performed to analyze the relationship between the expression of PIM-1 and EGFR. Finally, cervical cancer cell line Hela cells were cultured and treated by PIM-1 siRNA, and MTT assay and Pi/Annexin V assay were performed to explore the effects of PIM-1 siRNA on the growth and apoptosis ability of the Hela cells. Results: PIM-1 was significantly up-regulated in cervical cancer tissue compared to adjacent tissue, and the expression of PIM-1 in patients with cervical cancer is positively associated with the size and metastasis of the tumor. ROC analysis showed PIM-1 is a sensitive biomarker for the diagnosis of cervical cancer. Furthermore, EGFR was over-expressed in cervical cancer tumor tissues, and the levels of PIM-1 and EGFR in cervical cancer tissue were positively correlated. Finally, PIM-1 siRNA dramatically inhibited the viability and promoted the apoptosis of the Hela cells. Conclusion: Our findings prove that PIM-1 may function as an oncogene in cervical cancer and can regulate the EGFR signaling in cervical cancer.

Effects of Papaya Leaf Extract (Carica papaya L.) on Cellular Proliferation and Apoptosis in Cervical Cancer Mice Model

2019 ◽  
Vol 17 (5) ◽  
pp. 265-275
Author(s):  
Y. Peristiowati ◽  
Y. Puspitasari ◽  
Indasah
Keyword(s):  
Cervical Cancer ◽  
Cell Proliferation ◽  
Hela Cells ◽  
Leaf Extract ◽  
Caspase 3 ◽  
Methanol Extract ◽  
Negative Control ◽  
Proliferation Index ◽  
Control Group ◽  
P53 Expression

This study is aimed at analyzing the anticancer properties of papaya leaf extract, specifically the inhibition of cell proliferation and apoptotic induction through nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) and p53 pathways. Twenty-five mice (Mus musculus), aged 2 months and weighing 20–30 g, was injected with 0.5 mg dexamethasone for 7 days. The mice were then injected intracutaneously with 1 ml of HeLa cells (8 × 106 HeLa cells/microliter). The mice were divided into five groups (5 each): negative control (P1) (5% CMC-Na, sodium carboxymethyl cellulose), treatment II (225 mg/kg BW (body weight) papaya leaves methanol extract), treatment III (450 mg/kg BW), treatment IV (750 mg/kg BW), and treatment PV (2 mg alcohol anticancer drug). Papaya leaf extract treatments were applied for 2 weeks. Then, the tumor tissue was isolated for hematoxylin and eosin staining. Immunohistochemical imaging was used to detect Ki-67, caspase-3, NF-κB, and p53 expression. Further analysis was undertaken using the ImmunoRatio software program. The results indicated that administration of papaya leaf methanol extract significantly increased the expression of NF-κB and p53 at a dose of 450 mg/kg BW. Our results also showed that the mice treated with 450 mg of papaya leaf extract per kg of BW (P3) had the largest increase of caspase-3 expression compared to the negative control group. Papaya leaf ethanol extract decreased the cancer cell proliferation index and increased apoptosis of cancer cells in animal models of cervical cancer; it may also work to increase NF-kB expression and expression of the p53 gene.

Sign in / Sign up

Export Citation Format

Share Document