COMPARATIVE DESTRUCTION OF LACTIC STREPTOCOCCUS BACTERIOPHAGES ON VARIOUS SURFACES BY GERMICIDAL AEROSOLS1,2

Summary A comparison was made of destruction by various germicidal aerosols of lactic streptococcus bacteriophages on a number of different types of representative building surfaces and stainless steel. With the exception of the stainless steel the surfaces were coated either with enamel or epoxy resin. Surfaces were contaminated by fogging with a phage suspension in a test chamber. After 15 min, germicide was fogged into the chamber and phage recovered from surfaces by scrubbing with inactivator solution. Chlorine compounds as represented by dichloroisocyanuric acid (DCCA) and sodium hypochlorite (NaOCl) were markedly superior to quaternary ammonium compound (QAC) and phosphoric acid wetting agent (PAWA). Porosity of surface greatly influenced rate of phage inactivation. Aerosols appeared to be most effective on surfaces in the horizontal as compared to the vertical position. Complete inactivation of phage was not realized with either QAC or PAWA at all concentrations tested. However, fogging levels of 2000 ppm DCCA and NaOCl attained 100% of destruction of bacteriophage on horizontal building surfaces and 500 to 1000 ppm accomplished this on stainless steel.

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Effectiveness of Sanitation with Quaternary Ammonium Compound or Chlorine on Stainless Steel and Other Domestic Food-Preparation Surfaces

Journal of Food Protection ◽

10.4315/0362-028x-60.1.43 ◽

1997 ◽

Vol 60 (1) ◽

pp. 43-47 ◽

Cited By ~ 54

Author(s):

JOSEPH F. FRANK ◽

REVIS A. N. CHMIELEWSKI

Keyword(s):

Stainless Steel ◽

Quaternary Ammonium ◽

Test Procedure ◽

Food Service ◽

Quaternary Ammonium Compound ◽

Ammonium Compound ◽

Relative Ability ◽

Polished Stainless Steel ◽

Materials Used ◽

Type 304

The relative ability of various materials used for domestic and/or food-service sinks and countertops to be sanitized was determined. Both smooth (unused) and abraded surfaces were tested by exposure to 200 mg of quaternary ammonium compound per liter or 200 mg of sodium hypochlorite per liter. Surface materials tested included mechanically polished (type 304, #4 finish) and electropolished stainless steel, polycarbonate, and mineral resin. Surfaces were prepared for testing by allowing attachment of a Staphylococcus aureus culture for 4 h to achieve an initial attached population of 104 to 105 CFU/cm2. The test procedure involved immersion of the surface in sanitizer solution followed by wiping with a sanitizer-saturated cloth. Residual staphylococci were detected by overlaying agar directly on the treated surface. Results indicated that the stainless steels and the smooth polycarbonate, which had 0.5 log CFU/cm2 or fewer of residual staphylococci, were more readily sanitized by quaternary ammonium compound than were either the mineral resin surfaces, which had nearly 2.0 log CFU/cm2 of residual staphylococci, or the abraded polycarbonate which had nearly 1.0 log CFU/cm2 of residual staphylococci. Chlorine was most effective on the mechanically polished stainless steel, the unabraded electropolished stainless steel, and the polycarbonate surfaces, reducing cell populations to less than 1.0 log CFU/cm2. Chlorine was less effective on abraded electropolished stainless steel and mineral resin surfaces, where populations remained greater than 1.0 log CFU/cm2. Sanitation with quaternary ammonium compound or chlorine reduced S. aureus populations more than 1,000-fold on all surfaces except unabraded mineral resin.

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Inactivation of Listeria monocytogenes by Disinfectants and Bacteriophages in Suspension and Stainless Steel Carrier Tests

Journal of Food Protection ◽

10.4315/0362-028x.jfp-14-151 ◽

2014 ◽

Vol 77 (12) ◽

pp. 2012-2020 ◽

Cited By ~ 27

Author(s):

N. CHAITIEMWONG ◽

W. C. HAZELEGER ◽

R. R. BEUMER

Keyword(s):

Stainless Steel ◽

Listeria Monocytogenes ◽

Quaternary Ammonium ◽

Antimicrobial Effect ◽

Quaternary Ammonium Compound ◽

Steel Plates ◽

Ammonium Compound ◽

Standard Requirement ◽

Sodium Dichloroisocyanurate ◽

Food Residues

To simulate food contact surfaces with pits or cracks, stainless steel plates with grooves (depths between 0.2 and 5 mm) were constructed. These plates were artificially contaminated with Listeria monocytogenes in clean conditions, with organic soiling, or after 14 days of biofilm formation after which inactivation of the pathogen by Suma Tab D4 (sodium dichloroisocyanurate, 240 and 300 mg/liter), Suma Bac D10 (quaternary ammonium compound, 740 mg/liter), and bacteriophage suspension (Listex P100) was determined. Both chemical disinfectants performed well in suspension tests and in clean carrier tests according to the European standard with a reduction of more than 5 and 4 log units, respectively, of Listeria cells after 5 min of contact time. However, for the plates with grooves, the reduction could not meet the standard requirement, although a higher reduction of L. monocytogenes was observed in the shallow grooves compared with the deeper grooves. Furthermore, presence of food residues and biofilm reduced the effect of the disinfectants especially in the deep grooves, which was dependent on type of food substrate. Bacteriophages showed the best antimicrobial effect compared with the chemical disinfectants (sodium dichloroisocyanurate and quaternary ammonium compound) in most cases in the shallow grooves, but not in the deep grooves. The chlorine based disinfectants were usually less effective than quaternary ammonium compound. The results clearly demonstrate that surfaces with grooves influenced the antimicrobial effect of the chemical disinfectants and bacteriophages because the pathogen is protected in the deep grooves. The use of bacteriophages to inactivate pathogens on surfaces could be helpful in limited cases; however, use of large quantities in practice may be costly and phage-resistant strains may develop.

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COMPARATIVE DESTRUCTION OF AIR-BORNE LACTIC BACTERIOPHAGES BY VARIOUS GERMICIDES APPLIED AS AEROSOLS1,2

Journal of Milk and Food Technology ◽

10.4315/0022-2747-27.5.129 ◽

1964 ◽

Vol 27 (5) ◽

pp. 129-134 ◽

Cited By ~ 4

Author(s):

E. L. Sing ◽

P. R. Elliker ◽

W. E. Sandine

Keyword(s):

Phosphoric Acid ◽

Quaternary Ammonium ◽

Chelating Agents ◽

Quaternary Ammonium Compound ◽

Ammonium Compound ◽

Virucidal Activity ◽

High Concentration ◽

Experimental Conditions ◽

Ammonium Compounds ◽

Dichloroisocyanuric Acid

Summary The chlorine-releasing compounds were highly effective in destroying air-borne lactic streptococcus bacteriophages 144F and 18–16. At a concentration of .048 ppm available chlorine in the chamber, which was attained with 1000 ppm available chlorine in the solution applied, the inactivation was 99.999% or better. Of the various germicides tested, the quaternary ammonium compound and phosphoric acid wetting agent were found to be least effective as virucidal aerosols. Incorporation of chelating agents (EDTA) with quaternary ammonium compounds, to enhance their virucidal activity, was unsuccessful. The effectiveness of iodophor applied as an aerosol, was progressively greater with increasing concentration; however, undesirable side effects such as a heavy brown residue on all surfaces contacted and undesirable odors were noted. The study of corrosive effects by germicides on various metals indicated that of the chlorine-containing compounds, dichloroisocyanuric acid was least corrosive. Phosphoric acid wetting agents were the least corrosive of all compounds tested. Results of this study suggested that the chlorine-containing compounds, when applied as an aerosol at sufficiently high concentration, should provide the most effective agents for sanitizing procedures for the control of bacteriophages in the air and on building and equipment surfaces. The results further indicated that a concentration of .048 ppm available chlorine or more in the air provided most effective phage destruction under experimental conditions. This concentration was provided by the equivalent of 1 to 1.5 liters of 1000 ppm applied as an aerosol per 1000 ft3 of space. Present state of knowledge would indicate that dichloroisocyanuric acid should be an effective and practical compound for control of bacteriophage in dairy plants. In addition to being highly virucidal, dichloroisocyanuric acid also has a low rate of corrosion.

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The Effect of Blood on the Antiviral Activity of Sodium Hypochlorite, a Phenolic, and a Quaternary Ammonium Compound

Infection Control and Hospital Epidemiology ◽

10.1086/501591 ◽

1999 ◽

Vol 20 (12) ◽

pp. 821-827 ◽

Cited By ~ 48

Author(s):

David J. Weber ◽

Susan L. Barbee ◽

Mark D. Sobsey ◽

William A Rutala

Keyword(s):

Sodium Hypochlorite ◽

Quaternary Ammonium ◽

Quaternary Ammonium Compound ◽

Final Concentration ◽

Ammonium Compound ◽

Complete Inactivation ◽

Test Organisms ◽

Viral Survival ◽

Simplex Virus

AbstractObjective:To assess the virucidal activity of three disinfectants (sodium hypochlorite, a phenolic, and a quaternary ammonium compound) in the presence and absence of blood.Methods:Disinfectants at varying concentrations (hypochlorite: 5,000, 500, or 50 ppm; phenolic: 1:10 or 1:128 dilution; quaternary ammonium compound: 1:10 or 1:128 dilution) were added to either saline or whole blood (final concentration, 80% or 20% blood) and mixed. Test organisms included an attenuated vaccine strain of poliovirus type 1 (prototype for relatively resistant hydrophilic viruses) and herpes simplex virus (HSV) type 1 (prototype for relatively susceptible lipophilic viruses). Virus was added to create a viral-blood suspension. Viral survival was tested at room temperature at the following times: 0, 15 seconds, 30 seconds, 1 minute, 2 minutes, 5 minutes, and 10 minutes. A neutralizer stopped the reaction, and virus was assayed using a plaque technique.Results:In the absence of blood, complete inactivation of HSV was achieved within 30 seconds with 5,000 (1:10 dilution of bleach) and 500 (1:100 dilution of bleach) ppm chlorine, 1:10 and 1:128 diluted phenolic (use dilution), and 1:10 and 1:128 diluted quaternary ammonium compound (use dilution). In the presence of 80% blood, only 5,000 ppm hypochlorite, 1:10 phenolic, and 1:10 or 1:128 quaternary ammonium compound were effective. In the absence of blood, complete inactivation of polio was achieved within 30 seconds by 5,000 and 500 ppm chlorine and 1:10 quaternary ammonium compound. In the presence of 80% blood, no solution tested was capable of completely inactivating poliovirus within 10 minutes.Conclusions:Our data suggest that, in the absence of visible blood, environmental surfaces may be disinfected with a diluted hypochlorite solution (1:10 or 1:100), a phenolic, or a quaternary ammonium compound. Based on our studies using HSV, which has similar susceptibilities to disinfectants as human immunodeficiency virus (HIV), phenolics at their use dilution and 1:100 diluted hypochlorite are unlikely to inactivate HIV or hepatitis B virus reliably in the presence of blood. Hypochlorite at a final concentration of 5,000 ppm (1:10 dilution) should be used to decontaminate blood spills, but, even after decontamination, care should be used to avoid sharps injuries.

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Sensitivity of Listeria monocytogenes to Sanitizers after Exposure to a Chemical Shock

Journal of Food Protection ◽

10.4315/0362-028x-59.4.374 ◽

1996 ◽

Vol 59 (4) ◽

pp. 374-378 ◽

Cited By ~ 10

Author(s):

EUREKA L. PICKETT ◽

ELSA A. MURANO

Keyword(s):

Stainless Steel ◽

Listeria Monocytogenes ◽

Inhibitory Concentration ◽

Quaternary Ammonium Compound ◽

Ammonium Compound ◽

Ph Adjustment ◽

Lethal Level ◽

Significant Difference ◽

Subsequent Exposure ◽

Steel Surfaces

We tested the hypothesis that exposure of Listeria monocytogenes to sublethal levels of sanitizers (chemical shock) could affect survival to a subsequent exposure to lethal levels and the ability of the cells to attach to stainless steel surfaces. L. monocytogenes was exposed to an acidic anionic sanitizer, a chlorine-based sanitizer, an iodophor, and a quaternary ammonium compound, as well as to citric, lactic, and propionic acids. The cells were exposed to sublethal levels of each sanitizer for up to 60 min (chemical shock), followed by exposure to either the minimum inhibitory concentration (MIC) for 48 h, to the lethal level for 48 h, or to the MIC for 40 min followed by the lethal level for 48 h. No significant difference in survival was observed with most of the sanitizers used. However, exposure to a chemical shock with the acid anionic sanitizer for at least 10 min resulted in survival of the cells in the MIC of this sanitizer, as well as in the lethal level, but only when the cells were first exposed to the MIC for 40 min. Deliberate dissociation of citric acid by pH adjustment also resulted in survival of chemically shocked cells to lethal levels of this acid, suggesting that exposure to the dissociated form somehow enabled cells to survive exposure to lethal levels of the acid. Chemical shock did not affect attachment of the cells to stainless-steel chips.

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Transfer of Persistent Listeria monocytogenes Contamination between Food-Processing Plants Associated with a Dicing Machine

Journal of Food Protection ◽

10.4315/0362-028x-65.7.1129 ◽

2002 ◽

Vol 65 (7) ◽

pp. 1129-1133 ◽

Cited By ~ 109

Author(s):

JANNE M. LUNDÉN ◽

TIINA J. AUTIO ◽

HANNU J. KORKEALA

Keyword(s):

Stainless Steel ◽

Listeria Monocytogenes ◽

Sodium Hypochlorite ◽

Quaternary Ammonium ◽

Quaternary Ammonium Compound ◽

Pfge Type ◽

Ammonium Compound ◽

Type I ◽

Minimal Inhibitory Concentrations ◽

Steel Surfaces

The possibility of the transfer of persistent Listeria monocytogenes contamination from one plant to another with a dicing machine was evaluated, and possible reasons for persistent contamination were analyzed. A dicing machine that diced cooked meat products was transferred from plant A to plant B and then to plant C. After the transfer of the dicing machine, L. monocytogenes PFGE type I, originally found in plant A, was soon also found in plants B and C. This L. monocytogenes PFGE type I caused persistent contamination of the dicing lines in plants B and C. The persistent L. monocytogenes strain and three nonpersistent L. monocytogenes strains found in the dicing line of plant C were tested for adherence to stainless steel surfaces and minimal inhibitory concentrations of a quaternary ammonium compound and sodium hypochlorite, disinfectants widely used in the dicing lines. The persistent strain showed significantly higher adherence to stainless steel surfaces than did the nonpersistent strains. The minimal inhibitory concentrations of sodium hypochlorite were similar for all strains, and the minimal inhibitory concentrations of the quaternary ammonium compound for three of the L. monocytogenes PFGE types, including the persistent PFGE type, were high. All persistent L. monocytogenes PFGE type I isolates were found in an area with high hygienic standards, with the dicing machine being the first point of contamination. These observations show that the dicing machine sustained the contamination and suggest that the dicing machine transferred the persistent L. monocytogenes PFGE type from one plant to another.

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Prevalence and Detection of qac Genes from Disinfectant-Resistant Staphylococcus aureus Isolated from Salon Tools in Ishaka Town, Bushenyi District of Uganda

Canadian Journal of Infectious Diseases and Medical Microbiology ◽

10.1155/2020/1470915 ◽

2020 ◽

Vol 2020 ◽

pp. 1-9

Author(s):

Solange Gahongayire ◽

Adamu Almustapha Aliero ◽

Charles Drago Kato ◽

Alice Namatovu

Keyword(s):

Sodium Hypochlorite ◽

Quaternary Ammonium ◽

Bacterial Infections ◽

Healthcare Providers ◽

Quaternary Ammonium Compound ◽

Diffusion Method ◽

Ammonium Compound ◽

Bacterial Strains ◽

Microbiological Methods ◽

Phenotypic Methods

Bacterial infections are on a rise with causal-resistant strains increasing the economic burden to both patients and healthcare providers. Salons are recently reported as one of the sources for transmission of such resistant bacterial strains. The current study aimed at the identification of the prevalent bacteria and characterization of quaternary ammonium compound (qac) genes from disinfectant-resistant S. aureus isolated from salon tools in Ishaka town, Bushenyi District of Uganda. A total of 125 swabs were collected from different salon tools (combs, brushes, scissors, clippers, and shaving machines), and prevalent bacteria were isolated using standard microbiological methods. Identification of isolated bacteria was done using standard phenotypic methods including analytical profile index (API). Susceptibility patterns of the isolated bacteria to disinfectant were determined using the agar well diffusion method. Quaternary ammonium compound (qac) genes (qacA/B and qacC) associated with disinfectant resistances were detected from disinfectant-resistant S. aureus using multiplex polymerase chain reaction (PCR) and Sanger sequencing methods. Of the 125 swab samples collected from salons, 78 (62.4%) were contaminated with different bacteria species. Among the salon tools, clippers had the highest contamination of 20 (80.0%), while shaving machines had the lowest contamination of 11 (44.0%). The most prevalent bacteria identified were Staphylococcus epidermidis (28.1%) followed by S. aureus (26.5%). Of all the disinfectants tested, the highest resistance was shown with sodium hypochlorite 1%. Out of the eight (8) disinfectant-resistant S. aureus analysed for qac genes, 2 (25%) isolates (STP6 and STP9) were found to be qacA/B positive, while 2 (25%) isolates (STP8 and STP9) were found to be qacC gene positive. This study has shown that bacterial contamination of salon tools is common, coupled with resistance to disinfectants with sodium hypochlorite resistance being more common. Furthermore, observed resistance was attributed to the presence of qac genes among S. aureus isolates. A search for qac genes for disinfectant resistance from other bacteria species is recommended.

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