MICROTITER METHOD FOR ENUMERATING VIABLE BACTERIA IN MILK

1969 ◽  
Vol 32 (4) ◽  
pp. 144-146 ◽  
Author(s):  
Daniel Y. C. Fung ◽  
William S. LaGrange
Keyword(s):  
New Method ◽  
Plate Count ◽  
Bacterial Cells ◽  
Practical Application ◽  
Standard Plate Count ◽  
Time Space ◽  
Standard Plate ◽  
Viable Bacteria ◽  
Plate Count Method ◽  
Space Material

A rapid microtiter method was investigated as a means of evaluating viable bacterial cells in milk samples, using the conventional Standard Plate Count method as a comparison. Statistical analyses showed high correlation between the two methods. The advantages of this new method are: (a) the ability to analyze many samples simultaneously and (b) savings of time, space, material, and labor. The practical application of this new method in dairy products was shown by a study of bacterial growth during refrigerated storage of milk.

Evaluation of a Membrane Filter Test Kit for Monitoring Bacterial Counts in Cannery Cooling Waters

1982 ◽  
Vol 45 (12) ◽  
pp. 1087-1090 ◽  
Author(s):  
C. R. REY ◽  
G. A. HALABY ◽  
E. V. LOVGREN ◽  
T. A. WRIGHT
Keyword(s):  
Standard Method ◽  
Membrane Filter ◽  
Plate Count ◽  
Bacterial Counts ◽  
Standard Plate Count ◽  
Test Kit ◽  
Standard Plate ◽  
Viable Bacteria ◽  
Plate Count Method ◽  
Filter Test

Performance of the Millipore SPC Sampler was compared with the Standard Plate count method for routine checks of bacterial counts in cannery cooling waters. Methods were tested with cooling waters from a hydrostatic retort. Recovery of viable bacteria was very low when the Millipore samples were incubated for 24 h, but incubation for 48 or 72 h consistently yielded higher counts with the Millipore than with the standard method. Duplication of counts between analysts was approximately equal with both methods. Replication of bacterial counts within samples was more erratic and skips were more frequent with the Millipore than with the standard method. Procedures to control replications and skips are discussed. The Millipore procedure is a convenient alternative to the Standard Plate Count for routine quality audit of cannery cooling waters.

scholarly journals Microbiological Profile of the Traditionally Collected Industrial Raw Milk from the Milk Pocket Zones of Bangladesh

1970 ◽  
Vol 25 (1) ◽  
pp. 17-20 ◽  
Author(s):  
Gouranga C Chanda ◽  
Gazi M Noor Uddin ◽  
Aparna Deb ◽  
Tahmina Bilkis ◽  
Sharmin Chowdhury ◽  
...  
Keyword(s):  
Bacterial Count ◽  
Raw Milk ◽  
Total Coliform ◽  
Plate Count ◽  
Coliform Count ◽  
Standard Plate Count ◽  
Milk Samples ◽  
Bacteriological Profile ◽  
Standard Plate ◽  
Viable Bacteria

The study was aimed to evaluate the bacteriological profile of the traditionally collected industrial raw milk from the milk pocket zones of Bangladesh. About 365 raw milk samples were collected from the milk tanker, who brought raw milk from the mother chilling centre where raw milk was chilled at 4°C following traditional method. All milk samples were subjected to perform standard plate count and total coliform count. The average standard plate count was found to be 4.37 x 106 cfu/ml and the highest occurrence of standard plate count was found to be 6.70 x 106 cfu/ml in October and the lowest (3.28 x 106 cfu/ml) in March. The highest occurrence of total viable bacteria was found to be 5.64 x 106 cfu/ml in autumn and the lowest was found to be 3.78 x 106 cfu/ml in summer. On the other hand, the average of the coliform bacterial count was found to be 3.88 x 105 cfu/ml with the highest (5.70 x 105 cfu/ml) occurrence in May and the lowest (1.90 x 105 cfu/ml) in January. Moreover, the highest occurrence of coliform count was found to be 4.84 x 105 cfu/ml in rainy season and the lowest was 2.75 x 105 cfu/ml found in winter.DOI: http://dx.doi.org/10.3329/bjm.v25i1.4849 Bangladesh J Microbiol, Volume 25, Number 1, June 2008, pp 17-20

Comparison of 3M™ Petrifilm™ Aerobic Count Plates to Standard Plating Methodology for Use with AOAC Antimicrobial Efficacy Methods 955.14, 955.15, 964.02, and 966.04 as an Alternative Enumeration Procedure: Collaborative Study

2013 ◽  
Vol 96 (4) ◽  
pp. 717-722 ◽  
Author(s):  
Maria T Nelson ◽  
Robert A LaBudde ◽  
Stephen F Tomasino ◽  
Rebecca M Pines ◽  
M Bennett ◽  
...  
Keyword(s):  
Collaborative Study ◽  
Test Methods ◽  
Antimicrobial Efficacy ◽  
Plate Count ◽  
Matched Pair ◽  
Acceptance Criterion ◽  
Standard Plate Count ◽  
Test Organisms ◽  
Standard Plate ◽  
Viable Bacteria

Abstract A multilaboratory study was conducted to determine the equivalence of the 3M™ Petrifilm™ Aerobic Count Plate and standard plating methodology for measuring viable bacteria and spores recovered from hard-surface carriers (stainless steel and porcelain), also known as "control carrier counts," used in AOAC antimicrobial efficacy test methods. Six laboratories participated in the study in which carriers inoculated with Staphylococcus aureus, Pseudomonas aeruginosa, Salmonella enterica, and spores of Bacillus subtilis were evaluated using 3M Petrifilm Aerobic Count (AC) plates and standard plating side-by-side. The data were analyzed using a matched-pair t-test to determine the between-method effect with confidence intervals. For all test organisms pooled across all laboratories, the mean difference in log10 concentration between the standard plate count method and 3M Petrifilm AC Plates was −0.012, with a 95% confidence interval of (−0.090, +0.066), which was well within the −0.5, +0.5 interval established as the acceptance criterion. The between-carrier SD averaged 0.139; the between-replicate SD was 0.050. The carrier reproducibility, given that a single replicate per carrier is done, was estimated to be 0.148. Although differences were seen in the final concentrations of the test organisms among laboratories, there were no statistical differences between the enumeration methods. Based on the results from this study, 3M Petrifilm AC Plates are equivalent to standard plating methodology and can be used as an alternative procedure for the enumeration of test organisms used in AOAC Methods 955.14, 955.15, 964.02, and 966.04.

scholarly journals Multiregional Evaluation of the SimPlate Heterotrophic Plate Count Method Compared to the Standard Plate Count Agar Pour Plate Method in Water

2000 ◽  
Vol 66 (1) ◽  
pp. 453-454 ◽  
Author(s):  
R. Wayne Jackson ◽  
Karen Osborne ◽  
Gary Barnes ◽  
Carol Jolliff ◽  
Dianna Zamani ◽  
...  
Keyword(s):  
Regression Analysis ◽  
Water Samples ◽  
Plate Count ◽  
Heterotrophic Plate Count ◽  
Plate Method ◽  
Standard Plate Count ◽  
Plate Count Agar ◽  
Count Method ◽  
Standard Plate ◽  
Plate Count Method

ABSTRACT A new SimPlate heterotrophic plate count (HPC) method (IDEXX Laboratories, Westbrook, Maine) was compared with the pour plate method at 35°C for 48 h. Six laboratories tested a total of 632 water samples. The SimPlate HPC method was found to be equivalent to the pour plate method by regression analysis (r = 0.95;y = 0.99X + 0.06).

Hydrophilicity and Bactericidal Effects of TiO2 Thin Films Prepared by RF Sputtering

2004 ◽  
Vol 449-452 ◽  
pp. 1261-1264 ◽  
Author(s):  
Yo Seung Song ◽  
Seon Hwa Kim ◽  
Bae Yeon Kim ◽  
Deuk Yong Lee
Keyword(s):  
Thin Films ◽  
Rf Sputtering ◽  
Angle Measurement ◽  
Wetting Angle ◽  
Plate Count ◽  
Tio2 Thin Films ◽  
Standard Plate Count ◽  
Bactericidal Effects ◽  
Standard Plate ◽  
Plate Count Method

TiO2 thin films were prepared by RF sputtering on Si wafer to investigate hydrophilicity and bactericidal effects by using AFM, XRD, standard plate count method and wetting angle measurement. Experimentally, the survival rate of E. coli was above 74% and the wetting angle of water for the 30 min UV illuminated films was 4.2o. It was found that the bactericidal efficiency was closely related to the large specific surface area caused by the roughness, however, hydrophilicity was governed by the surface defective sites rather than the roughness.

Microtiter Technique for Enumeration of Mesophiles, Psychrotrophs, and Coliforms in Raw and Pasteurized Milk

1977 ◽  
Vol 40 (11) ◽  
pp. 795-797 ◽  
Author(s):  
IVÀN A. CASAS ◽  
NELSON LEÓN ◽  
PEDRO IZQUIERDO
Keyword(s):  
Viable Cell ◽  
Limiting Factors ◽  
Plate Count ◽  
Microbial Quality ◽  
Pasteurized Milk ◽  
Standard Plate Count ◽  
Cell Counts ◽  
Count Method ◽  
Time Space ◽  
Standard Plate

The Microtiter Count Method was compared with the Standard Plate Count (SPC) method in evaluating mesophile, psychrotroph, and coliform counts for raw and pasteurized milk samples. Statistical analysis showed that the Microtiter Count Method was reliable when compared with the SPC for making viable cell counts on these products. The Microtiter Count Method is advantageous because it saves time, space, and material; this method should be useful for developing countries where availability of testing materials, manpower, and costs are limiting factors in surveillance of microbial quality.

Isolasi dan Seleksi Bakteri Pendegradasi Solar Dari Pelabuhan Penyeberangan Kendari – Wawonii, Sulawesi Tenggara

2019 ◽  
Vol 6 (2) ◽  
pp. 995
Author(s):  
M. Rajab Sutra Mijaya ◽  
Nur Arfa Yanti ◽  
Ardiansyah Ardiansyah ◽  
Nurhayani H. Muhiddin
Keyword(s):  
Diesel Fuel ◽  
Plate Count ◽  
Diesel Fuels ◽  
Standard Plate Count ◽  
Degrading Bacteria ◽  
Standard Plate ◽  
The Media ◽  
Plate Count Method ◽  
Selection Of ◽  
Rotary Shaker

This study aimed to obtain the ability of bacteria to degrade diesel fuel. Method of this research was exploration method. Bacteria were isolated by enrichment method used SMSSe enriched diesel 2% (v/v). Selection of hidrocarbonoclastic bacteria based on the ability of to grow on agar medium solid. The bacteria in the test made in the form of suspension with Mc Farland Standard 0.5. Test of bacterial isolates were used 10% of the inoculum put in 150 mL media with different concentrations of diesel fuel were 1%, 2% and 3% and incubated on a rotary shaker at 120 rpm. Samples were taken on 1, 5, 10, 15 and 20 days to test diesel emulsion by centrifugation at a speed of 3500 rpm ±15 minutes, the comparison between the media and diesel 4:1. Growth in the amount of bacteria accounted by a Standard Plate Count method. The levels of the diesel rest calculated every sampling during incubation. The selection results of obtained diesel degrading bacteria isolates that PSI.1 PSII.1 and PSIII.2. All of bacteria have the ability to degrade diesel fuel in different treatment, the best result in lowered diesel fuel that were a concentration of 3% during 20 days of incubated at PSII.1 isolate, that have the highest ability to reduce levels of diesel up to 70,70%.Keywords: Hydrocarbonoclastic, Degradation, Diesel fuels ABSTRAK Penelitian ini bertujuan untuk mengetahui kemampuan bakteri dalam mendegradasi solar. Penelitian ini adalah penelitian eksploratif. Isolasi bakteri menggunakan metode enrichment dengan media yang digunakan yaitu SMSSe yang diperkaya solar 2% (v/v). Pemilihan bakteri hidrokarbonoklastik berdasarkan kemampuan bakteri tumbuh pada media agar padat. Inokulum bakteri dibuat dalam bentuk suspensi dengan Standar Mc Farland 0,5. Pengujian kemampuan degradasi solar dilakukan menggunakan media minimal dengan variasi konsentrasi solar 1%, 2% dan 3% dan diinkubasi pada rotary shaker. Sampel diambil pada hari ke 1, 5, 10, 15 dan 20 untuk uji emulsi solar dengan menghitung volume solar yang teremulsi. Pertumbuhan jumlah bakteri dihitung dengan metode Standard Plate Count. Kadar sisa solar dihitung setiap pengambilan sampel selama inkubasi. Hasil seleksi bakteri pendegradasi solar diperoleh tiga isolat yaitu PSI.1 PSII.1 dan PSIII.2. Ketiga isolat bakteri memiliki kemampuan mendegradasi solar pada perlakuan yang berbeda, hasil terbaik dalam menurunkan kadar solar yaitu konsentrasi 3% selama 20 hari inkubasi pada isolat PSII.1 yang memiliki kemampuan tertinggi menurunkan kadar solar hingga 70,70%. Kata kunci : Hidrokarbonoklastik, Pendegradasi, Solar

scholarly journals THE POTENCY OF LOCAL ISOLATES FROM NORTH SUMATERA IN THE DEGRADATION OF NAPHTALENE

2015 ◽  
Vol 2 (1) ◽  
pp. 466
Author(s):  
Nunuk Priyani ◽  
Erman Munir ◽  
Irmalisyah W. Panjaitan ◽  
Kabul Warsito
Keyword(s):  
Carbon Source ◽  
Sole Carbon Source ◽  
Plate Count ◽  
Surface Compound ◽  
Standard Plate Count ◽  
Naphthalene Degradation ◽  
Standard Plate ◽  
Plate Count Method ◽  
Almost All ◽  
Hydrocarbon Compound

<p>The potency of biosurfactant–producing bacteria isolated from oil-contaminated coast, Belawan North Sumatera, in the degradation of hydrocarbon compound such as naphthalene has been studied. Thirteen isolates has been obtained using Bushnell Haas Agar containing 2% naphthalene as the sole carbon source. The bacterial growth was observed using Standard Plate Count method. The ability of each isolates to produce bioactive-surface compound was determined. A drop-collapsing test was performed to examine the activity of each biosurfactant. All isolates tested grew well in Bushnell Haas Broth medium. Among those, two isolates were able to remove almost all (99%) naphthalene from the medium during 15 days. Those two isolates have also shown highest concentration of biosurfactant production which is 75.180 ppm.</p><p><br /><strong>Keywords</strong>: local isolates, biosurfactant, naphthalene degradation.</p>

THE EFFECT OF FREEZING ON THE STANDARD PLATE COUNT OF MILK1

1955 ◽  
Vol 18 (12) ◽  
pp. 297-299 ◽  
Author(s):  
C. K. Johns ◽  
I. Berzins
Keyword(s):  
Plate Count ◽  
Rapid Freezing ◽  
Standard Plate Count ◽  
Count Method ◽  
Standard Plate ◽  
Plate Count Method

Milk and cream samples were subjected to (a) slow or (b) rapid freezing, and analysed after 24 and 48 hours storage by the standard plate count method. Bacterial destruction was much less than that reported for liquid egg.

Sign in / Sign up

Export Citation Format

Share Document