Antibiotic Susceptibility Patterns of Yersinia enterocolitica

1981 ◽  
Vol 44 (2) ◽  
pp. 124-127 ◽  
Author(s):  
L. RESTAINO ◽  
W. M. HILL

Antibiotic susceptibility patterns for Yersinia enterocolitica strains involving 10 different serotypes were analyzed and compared. All Y. enterocolitica were susceptible to colistin, gentamicin, kanamycin, neomycin and doxycycline, whereas all isolates displayed resistance to penicillin G, methicillin (derivative of penicillin), novobiocin, and clindamycin. The antibiograms for the Y. enterocolitica isolates were in some instances related to the somatic serotypes, especially serotype 0:8 for which the antimicrobial susceptibility pattern displayed the greatest disparity. By eliminating the antibiograms for the four serotype 0:8 strains, antimicrobial susceptibility patterns for atypical and typical strains were similar.

2018 ◽  
Vol 12 (10) ◽  
pp. 842-848
Author(s):  
Sukanya Sudhaharan ◽  
Padmaja Kanne ◽  
Padmasri Chavali ◽  
Lakshmi Vemu

Introduction: Pyogenic infections are an important cause of sepsis. These infections are difficult to treat because of the pathogens with increasing antibiotic resistance. It is important to know the pathogens causing the infections and its antibiotic susceptibility for proper management of the patients. Methodology: A retrospective analysis of 1428 culture positive pus and tissue samples received in the department of microbiology from various departments in the hospital between January 2012 to 2017 was performed. Data regarding the pathogen isolated and its antimicrobial susceptibility were collected and analyzed. The specimens were primarily processed, as per standard methods. Identification and susceptibility testing was done using the Vitek-2C system. Results: Among the samples males outnumbered females (M: F-2.5:1) and the median age was 47 years. The total number of patients were 1428 with total number of isolates being 1525 as in our study monomicrobial infections were seen in 93.2% (1331/1428) patients whereas combined infections with growth of two pathogens in 6.8% (97/1428). Gram-negative bacilli were isolated in 68.3% (1042/1525). Among the Gram-negative bacilli Escherichia coli was the major pathogen isolated (38.6%, 403/1042). Gram positive organisms were isolated in 31.6% (483/1525) of cases and Staphylococcus aureus was the predominant organism isolated (91.7%, 443/483). Rare pathogens like Burkholderia pseudomallei in 3 patients and Nocardia in one patient were also isolated. Conclusion: This study emphasizes to understand the common organisms isolated from wound infections and it helps in empirical treatment of patients based on antibiotic susceptibility patterns.


2014 ◽  
Vol 8 (06) ◽  
pp. 699-704 ◽  
Author(s):  
Hugo E Villar ◽  
Mónica B Jugo ◽  
Alejandro Macan ◽  
Matias Visser ◽  
Mariana Hidalgo ◽  
...  

Introduction: Knowledge of the etiology and antimicrobial susceptibility patterns of uropathogens is important for determining the best treatment option. This study aimed to determine the distribution and antibiotic susceptibility patterns of bacterial strains isolated from adult male outpatients. Methodology: Between November 2012 and April 2013, 3,105 community urine samples were analyzed from adult male patients who attended the Laboratorio Hidalgo, Buenos Aires, Argentina. Antimicrobial susceptibility testing was performed by the Kirby-Bauer disc diffusion method. Isolates resistant to third generation cephalosporin were tested for extended-spectrum beta-lactamase (ESBL) production using the double-disk synergy test. Results: Of the 3,105 urine samples analyzed, 791 (25.5%) had significant bacteriuria. The frequency of positive urine cultures increased significantly with patient age. Escherichia coli was isolated most frequently (47.3%), followed by Enterococcus faecalis (13.6%), and Klebsiella pneumoniae (11.9%). Gram-negative organisms represented 78.8% of urinary pathogens. The highest activities against Gram-negative bacteria were found with imipenem (99.0%), amikacin (98.1%), ertapenem (94.2%), fosfomycin (90.7%), and piperacillin-tazobactam (90.1%). The frequencies of ESBLs among E. coli, K. pneumoniae, and P. mirabilis were 15.2 %, 22.3%, and 8%, respectively. Fosfomycin, piperacillin-tazobactam, and nitrofurantoin were most effective against Gram-positive organisms. Conclusions: Fosfomycin may be an excellent option for cystitis treatment in patients without risk factors, whereas piperacillin-tazobactam is preferred for the treatment of parenchymatous UTIs, complicated UTIs, and UTIs associated with risk factors. To ensure the optimal selection of antibiotics, physicians should have access to up-to-date information about the local prevalence of antimicrobial resistance.


2020 ◽  
Author(s):  
JOHN MACHARIA MACHARIA ◽  
Zsolt Kaposztas ◽  
Cheruiyot Richard ◽  
Nyerere K. Andrew ◽  
Mathenge J. Muriithi ◽  
...  

Abstract Introduction: Staphylococcus aureus is a major foodborne pathogen that poses a serious threat to public health. Indiscriminate use of antibiotics increases evolution of antibiotic resistant strains. This study aimed to determine the presence of Staphylococcus aureus in milk and milk products and their antimicrobial susceptibility patterns. Methods: A total of 334 samples were collected for analysis in the laboratories. To determine antimicrobial susceptibility patterns, selected antibiotics from different classes were used: Penicillin G, Erythromycin, Vancomycin, Chloramphenicol, Tetracycline, Gentamycin, Methicillin, and Ciprofloxacin. Results: The presence of S. aureus in milk and milk products was found occurring in 21.56% of all the samples. In raw milk analyzed, 64.81% of samples were contaminated by Staphylococcus aureus, 20.54% in pasteurized milk, 10.71% in yogurt, and 3.57% in ice cream. All isolates were found to be 100 % sensitive to Tetracycline, Ciprofloxacin, Erythromycin, and Methicillin. Infrequent sensitivity was found in Gentamicin and Vancomycin. Resistance to Penicillin G was occasionally observed across the different sources of milk and milk products. Resistance to Gentamicin (42 %) and Vancomycin (11 %) was seldom observed in isolates, hence occurring in yogurt samples only. Conclusion and Recommendation: The research hypothesis was rejected based on the presence of pathogenic Staphylococcus aureus across the different samples analyzed. It is recommended that Tetracycline, Ciprofloxacin, Methicillin, and Erythromycin antibiotics should be used for the treatment of Staphylococcus aureus infections based on the susceptibility test outcome.


Author(s):  
Alpa Patel ◽  
Nirmal Choraria

serovars Typhi and Paratyphi are known to cause enteric fever. Multidrug resistance in and has emerged as a cause of concern. To evaluate antimicrobial susceptibility patterns of Salmonella enteric serovar Typhi () and obtained from blood culture.: All isolates obtained from blood cultures of clinically suspected cases of enteric fever coming to microbiology laboratory, Nirmal hospital, from January 2015 to September 2017 were included in the study. Antimicrobial susceptibility patterns were determined using commercial antimicrobial disks chloramphenicol (30 μg), nalidixic acid (30 μg), ampicillin (10 μg), azithromycin (15 μg), cotrimoxazole (1.25/23.75 μg), ciprofloxacin (5 μg), and ceftriaxone (30 μg). Antimicrobial susceptibility testing was performed in accordance with the Clinical and Laboratory Standards Institute (CLSI) guidelines of respective year by KirbyBauer disc diffusion method.: Total 330 isolates of salmonella are there out of that 298 is . 32 are Salmonella para A, while 1 is of Salmonella para B. Enteric fever cases pick month are April, May, June and July. Sensitivity to first line drugs are > 80%, Nalidixic acid resistant Salmonella (NARS) are 79%, while Multi drug resistant (resistant to ampicillin, chloramphenicol and co-trimoxazole all three)Salmonella are 3%.: Periodic evaluation of antibiotic susceptibility pattern is necessary to see changing pattern of antibiotics. Evaluation of Nalidixic acid resistant Salmonella and periodic evaluation of multi drug resistant Salmonella is also important as emergence of MDR strain is observed in our study.


2017 ◽  
Vol 61 (8) ◽  
Author(s):  
Manon Vouga ◽  
David Baud ◽  
Gilbert Greub

ABSTRACT In past years, several Chlamydia-related bacteria have been discovered, including Simkania negevensis, the founding member of the Simkaniaceae family. We evaluated the antimicrobial susceptibility patterns of this emerging intracellular bacterium and highlighted significant differences, compared with related Chlamydiales members. S. negevensis was susceptible to macrolides, clindamycin, cyclines, rifampin, and quinolones. Importantly, unlike other Chlamydiales members, treatment with β-lactams and vancomycin did not induce the formation of aberrant bodies, leading to a completely resistant phenotype.


2018 ◽  
Vol 10 (02) ◽  
pp. 140-144 ◽  
Author(s):  
Anitha Madhavan ◽  
Sobha Balakrishnan ◽  
Jayalakshmi Vasudevapanicker

ABSTRACT BACKGROUND AND OBJECTIVES: Shigellosis is one of the most common causes of morbidity and mortality in children in developing countries. To the best of our knowledge, there is no published data in the study area on the antimicrobial susceptibility pattern and prevalence of Shigella species among diarrheagenic cases. Therefore, a retrospective analysis was done to find the Shigella serotypes, common age group affected, and antimicrobial resistance pattern of Shigella isolates in South Kerala METHODS: Stool samples collected from cases of dysentery and diarrhea from January 2011 to December 2016 were processed. Standard bacteriological methods were used to isolate, identify, and determine the antimicrobial susceptibility pattern of Shigella isolates. The data were analyzed using SPSS version 16. RESULTS: Among 1585 stool samples, 48(3%) yielded Shigella . The most common serogroup isolated was Shigella sonnei (62.5%) followed by Shigella flexneri. Of 48 isolates, 44(91.6%) isolates were found to be multidrug resistant. Over the 5-year period, the isolates show 100% resistance to nalidixic acid, ciprofloxacin, and cotrimoxazole. Eight isolates were found to be resistant to ceftriaxone and cefotaxime. The presence of Extended spectrum betalactamase (ESBL) was phenotypically confirmed in five isolates. CONCLUSION: Even though S. flexneri is the most common Shigella-causing diarrhea, S. sonnei was found to be the most important species responsible in our study. Multidrug resistance was common (91.6%) and the most common multidrug resistance profile was ampicillin-nalidixic acid-cotrimoxazole-ciprofloxacin. Regular monitoring of antibiotic susceptibility pattern including detection of beta lactamases should be done in all microbiology laboratories. Guidelines for therapy should be monitored and modified based on regional susceptibility reports.


2018 ◽  
Vol 26 ◽  
pp. 61-66
Author(s):  
S Gul Nahar ◽  
M Bulbul Hasan ◽  
M Nawshad Ali ◽  
Mst Rokeya Khatun ◽  
K Khanam

Objective: The present study was done to see the antimicrobial susceptibility pattern of bacterial isolates from wound infection.Methods: Wound swabs collected from 150 patients of wound infection were cultured and microbial isolates identified using standard methods. Antimicrobial susceptibility testing was done on bacterial isolates by Modified Kirby Bauer method.Results: Of the 150 swabs 131 (87.4%) were culture positive for bacterial pathogens, while 19 (12.6%) were bacteriologically sterile showing an isolation rate of 87.4%. The predominant bacteria isolated from the infected wounds were Staphylococcus aureus 47 (32.4%) followed by Escherichia coli 29 (20%), Proteus species 23 (16%), Coagulase negative Staphylococci 21 (14.5%), Klebsiella pneumoniae 14 (10%) and Pseudomonas aeruginosa 11 (8%).In case of gram positive bacteria- rate of isolates resistant to ampicillin was 94%, followed by penicillin G, 86.8%, Tetracycline, 51.5%. In case of gram negative bacteria- rate of isolates resistant to ampicillin was 96%, followed by cephalothin, 92.4%, Tetracycline, 74%.Conclusion: In antimicrobial susceptibility testing ampicillin, penicillin, cephalothin and tetracycline were the least effective. Gentamicin, norfloxacin, ciprofloxacin, vancomycin and amikacin were the most effective antibiotics.TAJ 2013; 26: 61-66


Author(s):  
Sriram Govindaraj ◽  
Jinu Vadakkanethu Iype ◽  
Srinivasa V. ◽  
Jayendiran S.

<p class="abstract"><strong>Background:</strong> Chronic suppurative otitis media (CSOM) is one of the common infective conditions which can cause many complications if not treated properly. Improper use of antibiotic among the common population has led to many drug resistant organisms; therefore it is necessary to study the antimicrobial susceptibility pattern before treating the patient with CSOM. Aim of the study was to identify the bacterial isolates causing CSOM in tubo tympanic disease and to study their antimicrobial susceptibility pattern.</p><p class="abstract"><strong>Methods:</strong> The study was carried out in ENT department in Vinayaka Mission Medical College, Karaikal from July 2018 till December 2018 for six months. 60 patients of CSOM (TTD) with ear discharge attending the ENT Out Patient Department were included in the study. Swabs were collected for culture and sensitivity. Bacterial isolates were identified using standard methods and antibiotic susceptibility testing were done.  </p><p class="abstract"><strong>Results:</strong> Pseudomonas species was the most predominant organism, which was seen in 19 patients (38%) followed by <em>Escherichia coli</em> (22%), <em>Staphylococcus aureus</em> (18%), <em>Klebsiella species</em> (10%).</p><p class="abstract"><strong>Conclusions:</strong> A continuous and periodic evaluation of microbiological pattern and their antibiotic sensitivity pattern prevalent in local area, helps in prescribe proper antibiotics for successful treatment of CSOM and thus minimizing its complications and emergence of resistant strains.</p><p class="abstract"> </p>


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