Effect of Cleaners and Sanitizers on Listeria monocytogenes Attached to Product Contact Surfaces

1992 ◽  
Vol 55 (4) ◽  
pp. 246-251 ◽  
Author(s):  
E. P. KRYSINSKI ◽  
L. J. BROWN ◽  
T. J. MARCHISELLO
Keyword(s):  
Stainless Steel ◽  
Listeria Monocytogenes ◽  
Hazard Analysis ◽  
Control Point ◽  
Adherent Cells ◽  
Chemical Cleaning ◽  
Critical Control Point ◽  
Biofilm Removal ◽  
Good Manufacturing Practices ◽  
Conveyor Belts

A variety of chemical cleaning and sanitizing compounds were evaluated for their ability to remove and/or inactivate surface adherent Listeria monocytogenes. Adherent cells were obtained by incubating 1-cm2 chips of stainless steel or plastic conveyor belts with a multistrain cocktail of L. monocytogenes for 24 h at 25°C. Resistance of adherent cells to sanitizers was dependent upon the surface studied, being greatest on polyester/polyurethane followed by polyester and stainless steel. Biofilm removal with cleaners followed the same pattern as sanitizers with the polyester/polyurethane surface being most difficult to clean. Complete biofilm removal and/or inactivation was obtained in many cases where the surface was first cleaned prior to exposure to sanitizer. The data support conventional wisdom in that cleaning must precede sanitizing in order to remove and inactivate microorganisms. Listeria biofilms should be controllable by combining Good Manufacturing Practices with the discipline of a Hazard Analysis Critical Control Point Program.

scholarly journals Prevalensi dan Tingkat Kontaminasi Listeria monocytogenes di Tambak dan Unit Pengolahan Udang Vaname (Litopenaeus vannamei) untuk Pasar Ekspor

2021 ◽  
Vol 16 (2) ◽  
pp. 83
Author(s):  
Yusma Yennie ◽  
Gunawan Gunawan ◽  
Farida Ariyani
Keyword(s):  
Listeria Monocytogenes ◽  
Hazard Analysis ◽  
Control Point ◽  
Raw Material ◽  
Fish Processing ◽  
Shrimp Culture ◽  
Critical Control Point ◽  
Traceability System ◽  
Good Manufacturing Practices ◽  
Frozen Shrimp

Listeria monocytogenes adalah salah satu bakteri patogen yang dapat menyebabkan penyakit bawaan pangan. Penolakan ekspor produk udang beku Indonesia karena kontaminasi L. monocytogenes masih terjadi yang berdampak pada kerugian material bagi pelaku usaha. Tujuan penelitian ini adalah untuk mengetahui prevalensi dan tingkat kontaminasi L. monocytogenes pada produk udang beku untuk pasar ekspor. Sampel yang diambil merupakan udang segar dari tambak dan bahan baku dari bagian penerimaan di Unit Pengolahan Ikan (UPI) serta udang beku sebagai produk akhir UPI, dengan menerapkan sistem ketertelusuran. Lokasi penelitian adalah Sumatra Utara (Medan), DKI Jakarta, Jawa Timur (Surabaya dan Banyuwangi), dan Sulawesi Selatan (Makassar). Identifikasi dan enumerasi L. monocytogenes dilakukan dengan metode MPN-PCR dengan target gen hlyA (~456bp). Prevalensi L. monocytogenes pada udang vaname secara keseluruhan sebesar 6,7% (9/135 sampel), dengan prevalensi di masing-masing titik pengambilan sampel berturut-turut 6,1% di tambak, 9,6% di bahan baku, dan 4% di produk akhir, yang merupakan sampel udang dari batch yang sama. Tingkat kontaminasi L. monocytogenes pada sampel udang vaname berkisar 6,1-1.100 APM/g. Persyaratan L. monocytogenes pada bahan pangan adalah negatif/25g, sehingga sampel udang yang terkontaminasi L. monocytogenes tersebut tidak memenuhi persyaratan sebagai pangan yang aman untuk dikonsumsi berdasarkan regulasi yang berlaku di Indonesia maupun di negara lain. Kontaminasi L. monocytogenes pada udang beku kemungkinan berasal dari tambak ataupun lingkungan pengolahan. Penerapan Good Aquaculture Practices (GAP) di lingkungan tambak udang, serta Hazard Analysis Critical Control Point (HACCP) dan Good Manufacturing Practices (GMP) di UPI perlu dilakukan dengan benar sebagai upaya pengendalian kontaminasi L. monocytogenes. Selain itu, perlu dilakukan kajian lebih lanjut mengenai sumber dan titik kritis kontaminasi L. monocytogenes di sepanjang rantai pengolahan udang beku mulai dari tambak sampai produk akhir.ABSTRACTListeria monocytogenes is pathogenic bacteria that can cause foodborne illness. Rejection of frozen shrimp exports due to L. monocytogenes contamination still occurs and causes economical losses for the industries. The objective of this study was to determine the prevalence and the level of L. monocytogenes contamination in frozen shrimp for export markets. Samples collected were fresh shrimp from shrimp culture and raw material from the receiving point of fish processing plants (UPI), and frozen shrimp as the end product, by implementing a traceability system. Study locations were in North Sumatra (Medan), Special Capital Region of Jakarta, East Java (Surabaya dan Banyuwangi), and South Sulawesi (Makassar). Identification and enumeration of L. monocytogenes were carried out using the MPN-PCR method with the target gene hlyA (~456bp). The prevalence of L. monocytogenes in vanname shrimp was 6.7% (9 out of 135 samples), where 6.1%, 9.6%, and 4% of the prevalence were found in samples from shrimp culture, raw material, and end product, respectively. These samples were from the same batch. The contamination level ranged from 6.1 to 1,100 MPN/g. L. monocytogenes in food should be negative/25g, thus the contaminated samples do not meet requirements as safe for human consumption based on food regulation in Indonesia and other countries. Findings from this study suggested that shrimp culture or fish processing environment are potential sources of L. monocytogenes contamination in frozen shrimp. Therefore, the implementation of Good Aquaculture Practices (GAP) in shrimp culture environment, as well as Hazard Analysis Critical Control Point (HACCP) and Good Manufacturing Practices (GMP) in shrimp processing plant are necessary to control L. monocytogenes contamination. Further studies regarding the sources and critical points of L. monocytogenes contamination throughout the processing of frozen shrimp from shrimp culture to end product are also needed.

Food Safety Objectives for Listeria monocytogenes in Spanish Food Sampled in Cafeterias and Restaurants

2011 ◽  
Vol 74 (9) ◽  
pp. 1569-1573 ◽  
Author(s):  
E. DOMÉNECH ◽  
J. A. AMORÓS ◽  
I. ESCRICHE
Keyword(s):  
Listeria Monocytogenes ◽  
Food Safety ◽  
Safety Management ◽  
Hazard Analysis ◽  
Control Point ◽  
Consumer Health ◽  
Critical Control Point ◽  
Good Manufacturing Practices ◽  
Microbiological Criteria ◽  
Insight Into

To gain more insight into the context of food safety management by public administrations, food safety objectives must be studied. The Valencian administration quantified the prevalence of Listeria monocytogenes in cafeterias and restaurants in this region of Spain between 2002 and 2010. The results obtained from this survey are presented here for 2,262 samples of fish, salad, egg, cold meat, and mayonnaise dishes. Microbiological criteria defined for L. monocytogenes were used to differentiate acceptable and unacceptable samples; more than 99.9% of the samples were acceptable. These findings indicate that established food safety objectives are achievable, consumer health at the time of consumption can be safeguarded, and food safety management systems such as hazard analysis critical control point plans or good manufacturing practices implemented in food establishments are effective. Monitoring of foods and food safety is an important task that must continue to reduce the current L. monocytogenes prevalence of 0.1% in restaurant or cafeteria dishes, which could adversely affect consumer health.

Modeling the Effect of Storage Temperatures on the Growth of Listeria monocytogenes on Ready-to-Eat Ham and Sausage

2015 ◽  
Vol 78 (9) ◽  
pp. 1675-1681 ◽  
Author(s):  
KE LUO ◽  
SUNG-SAM HONG ◽  
DEOG-HWAN OH
Keyword(s):  
Listeria Monocytogenes ◽  
Hazard Analysis ◽  
Control Point ◽  
Meat Products ◽  
Quantitative Microbial Risk Assessment ◽  
Zone Method ◽  
Critical Control Point ◽  
Critical Control Points ◽  
Primary Model ◽  
Two Samples

The aim of this study was to model the growth kinetics of Listeria monocytogenes on ready-to-eat ham and sausage at different temperatures (4 to 35°C). The observed data fitted well with four primary models (Baranyi, modified Gompertz, logistic, and Huang) with high coefficients of determination (R2 > 0.98) at all measured temperatures. After the mean square error (0.009 to 0.051), bias factors (0.99 to 1.06), and accuracy factors (1.01 to 1.09) were obtained in all models, the square root and the natural logarithm model were employed to describe the relation between temperature and specific growth rate (SGR) and lag time (LT) derived from the primary models. These models were validated against the independent data observed from additional experiments using the acceptable prediction zone method and the proportion of the standard error of prediction. All secondary models based on each of the four primary models were acceptable to describe the growth of the pathogen in the two samples. The validation results indicate that the optimal primary model for estimating the SGR was the Baranyi model, and the optimal primary model for estimating LT was the logistic model in ready-to-eat (RTE) ham. The Baranyi model was also the optimal model to estimate the SGR and LT in RTE sausage. These results could be used to standardize predictive models, which are commonly used to identify critical control points in hazard analysis and critical control point systems or for the quantitative microbial risk assessment to improve the food safety of RTE meat products.

scholarly journals Assessment of the extent implementing the Good Manufacturing Practices (GMPs) and Hazard Analysis Critical Control Point System (HACCP) in Sudan sugar industry factories

2021 ◽  
pp. 368-373
Author(s):  
Babeker AM ◽  
Ahmed AI ◽  
Ahmed AR ◽  
Ebrahiem Mohammed Alhadi
Keyword(s):  
Food Safety ◽  
Sugar Industry ◽  
Hazard Analysis ◽  
Control Point ◽  
Point System ◽  
Critical Control Point ◽  
Critical Control Points ◽  
Good Manufacturing Practices ◽  
White Nile ◽  
Safety Systems

The present study was conducted in Sudan Sugar industry factories (Kenana, White Nile, Assalaya, Sennar, Guneid, and New Halfa) during the production season of 2017. The study aimed to evaluate the existing Assessment of the extent of implementing the Good Manufacturing Practices (GMPs) and Hazard Analysis Critical Control Point System (HACCP) by Sudanese sugar factories with reference to FSMS of the ISO 22000: 2005. The data were collected using ISO 22000 checklist and direct interviews. Likert Five-Point Scale was used in scoring the checklist statement and the arithmetic mean was obtained. The result showed that the Good Manufacturing Practices were varied between major deficiencies in White Nile, Assalaya, Sennar, and New Halfa due to score less than (3.6 points) and non-compliance in Kenana and Guneid due to score less than (2.6 points); the Sanitation Standard Operation Procedures (SSOP) assessed as major deficiencies in (Assalaya and Sennar) and minor deficiencies in (White Nile and New Halfa) due to score less than (5points). Moreover, the Hazard Analysis and Critical Control Points System revealed that it was varied between non-compliance in (Kenana, White Nile, and Guneid) and not applicable in (Assalaya, Sennar and New Halfa) due to score less than (1.8 points). Interestingly, the Sudanese sugar industries take all quality control characteristics from cane production to sugar production carefully, but unfortunately don’t care about any food safety systems. Lack of top management commitment and involvement and lack of government support represent the main constraints and Barriers of implementing food safety systems in Sudanese sugar companies. The study recommendations that the Sudanese Sugar Industry needs to establish proper quality assurance laboratories to help in monitoring the quality and safety of raw materials and end productions.

Validation of a 5-Log10 Reduction of Listeria monocytogenes following Simulated Commercial Processing of Lebanon Bologna in a Model System

2001 ◽  
Vol 64 (6) ◽  
pp. 873-876 ◽  
Author(s):  
NAVEEN CHIKTHIMMAH ◽  
ROBERT B. GUYER ◽  
STEPHEN J. KNABEL
Keyword(s):  
Listeria Monocytogenes ◽  
Hazard Analysis ◽  
Control Point ◽  
Meat Products ◽  
Model System ◽  
Product Recalls ◽  
Critical Control Point ◽  
Poultry Products ◽  
Commercial Process ◽  
Inspection Service

Recently, numerous product recalls and one devastating outbreak that claimed 21 lives were attributed to Listeria monocytogenes in ready-to-eat meat products. Consequently, the Food Safety and Inspection Service published a federal register notice requiring manufacturers of ready-to-eat meat and poultry products to reassess their hazard analysis and critical control point plans for these products as specified in 9 CFR 417.4(a). Lebanon bologna is a moist, fermented ready-to-eat sausage. Because of undesirable quality changes, Lebanon bologna is often not processed above 48.9°C (120°F). Therefore, the present research was conducted to validate the destruction of L. monocytogenes in Lebanon bologna batter in a model system. During production, fermentation of Lebanon bologna to pH 4.7 alone significantly reduced L. monocytogenes by 2.3 log10 CFU/g of the sausage mix (P < 0.01). Heating the fermented mix to 48.9°C in 10.5 h destroyed at least 7.0 log10 CFU of L. monocytogenes per g of sausage mix. A combination of low pH (5.0 or lower) and high heating temperatures (≥43.3°C, 115°F) destroyed more than 5 log10 CFU of L. monocytogenes per g of sausage mix during the processing of Lebanon bologna. In conclusion, an existing commercial process, which was validated for destruction of Escherichia coli O157:H7, was also effective for the destruction of more than 5 log10 CFU of L. monocytogenes.

Destruction of Listeria monocytogenes Biofilms on Stainless Steel Using Monolaurin and Heat1

1995 ◽  
Vol 58 (3) ◽  
pp. 251-255 ◽  
Author(s):  
DEOG-HWAN OH ◽  
DOUGLAS L. MARSHALL
Keyword(s):  
Stainless Steel ◽  
Listeria Monocytogenes ◽  
Food Industry ◽  
Adherent Cells ◽  
Planktonic Cells ◽  
Antimicrobial Effects ◽  
Biofilm Removal ◽  
Nutrient Environment ◽  
Physical Treatments

Individual and combined antimicrobial effects of monolaurin and heat on planktonic, 1-day adherent, or 7-day adherent cells of Listeria monocytogenes were determined to evaluate biofilm removal from stainless steel. Planktonic cells were more sensitive to heat and monolaurin than were cells attached to stainless steel. Young (1-day) biofilm cells were more sensitive to each treatment than were old (7-day) biofilm cells. Adherent cells were destroyed by 50 μg/ml monolaurin combined with heating at 65°C for 5 min. Cells in a rich nutrient environment were more resistant to treatment than were cells in a depleted nutrient environment. Results demonstrate the usefulness of combining chemical and physical treatments to control L. monocytogenes biofilm problems in the food industry.

scholarly journals PENERAPAN GOOD LOGISTIC PRACTICES UNTUK PRODUK PERIKANAN

2017 ◽  
Vol 3 (2) ◽  
pp. 129
Author(s):  
Winiati Pudji Rahayu ◽  
Wibisono Adhi
Keyword(s):  
Fishery Management ◽  
Hazard Analysis ◽  
Control Point ◽  
Quality And Safety ◽  
Chain Management ◽  
Safety Control ◽  
Resources Management ◽  
Critical Control Point ◽  
Good Manufacturing Practices ◽  
Product Quality And Safety

The utilization of Indonesia’s fisheries potential should be supported by the development of fisheries industry, more specifically by the improvement on fisheries supply chain management. It is needed to preserve the quality and safety of fisheries product from sea to consumers’ table and the sustainable fisheries supply. The fisheries product is perishable therefore the appropriate handling and full controlled process are needed to maintain product quality and safety. In fisheries industry, quality and safety can be maintained by applying good logistic practices (GLoP). GLoP is best practices ofcold chain management supported by quality and safety control system such as good manufacturing practices (GMP), standard sanitation operating procedure (SSOP) and hazard analysis critical control point (HACCP). On the other hand, the sustainability of fisheries supply canbe achieved by applying full controlled resources management. The commitment and cooperation of all parties on fisheries industry, as well as the standard implementation of fishery management is needed to improve quality and quantity of Indonesia’s fisheries products.

scholarly journals Pengaruh Penerapan Good Manufacturing Practices (GMP) Dan Penyusunan Sanitasi Standar Operasional Prosedur (SSOP) terhadap Proses Pengolahan Cumi Beku yang Dimoderasi oleh Sistem Hazard Analysis Critical Control Point (HACCP) pada PT. Sanjaya Internasional Fishery

Jurnal EBI ◽  
2021 ◽  
Vol 3 (1) ◽  
Author(s):  
Ari Soeti Yani ◽  
Riana Wulan Safitri
Keyword(s):  
Hazard Analysis ◽  
Control Point ◽  
Operational Procedure ◽  
Critical Control Point ◽  
Good Manufacturing Practices ◽  
Haccp System

This Study aims to determine the effect of the application of Good Manucturing Practices (GMP) and the preparation of Sanitation Standard Operational Procedure (SSOP) on the processing of frozen squid, and determine the effect of the Hazard Analysis Critical Control Point (HACCP) system as a moderating variable. The results of the research that love been carried out show the influence of the implementation of Good Manufacturing Practices (GMP), preparation of Sanitasi Standard Operational Procedure (SSOP) and Hazard Analysis Critical Control Point (HACCP) on the processing frozen squid. However, moderating the Hazard Analysis Critical Control Point (HACCP) is not able to moderate the Good Manufacturing Practices (GMP) and Sanitation Standard Operational Procedure of the frozen squid processing. From this research, it is explained one of the effects is that there are still irregularities in craying out the application of Good Manufacturing Practices (GMP), Sanitation Standard Operational Procedure (SSOP) and Hazard Analysis Critical Control Point (HAACP) to the frozen squid processing.

Effect of Inhibitory Liquid Smoke Fractions on Listeria monocytogenes during Long-Term Storage of Frankfurters

2007 ◽  
Vol 70 (2) ◽  
pp. 386-391 ◽  
Author(s):  
SARITHA GEDELA ◽  
J. ROY ESCOUBAS ◽  
PETER M. MURIANA
Keyword(s):  
Listeria Monocytogenes ◽  
Hazard Analysis ◽  
Health Hazard ◽  
Control Point ◽  
Effective Control ◽  
Potential Health ◽  
Critical Control Point ◽  
Liquid Smoke ◽  
Long Term Storage ◽  
Commercial Device

Listeria monocytogenes is a potential health hazard that sometimes finds harborage in facilities that manufacture ready-to-eat meats, including frankfurters. Our objectives were to examine the effect of select liquid smoke extracts on control of L. monocytogenes on frankfurters. Frankfurters were either obtained locally at retail (containing lactate-diacetate) or manufactured for us in-house or by a local processor (without added lactate-diacetate). In challenge studies of retail franks containing lactate-diacetate, low levels of L. monocytogenes were able to increase by 2 to 8 log on 5 of 10 brands tested when held at 1.6°C (35°F). Treatments with liquid smoke extracts were able to reduce and control growth of L. monocytogenes on the most permissive franks for 10 weeks when treated for as long as 90 s to as little as 5 s versus untreated controls. Effective control of L. monocytogenes was also obtained when dipped for as short as 1 s or when dropped through an atomized mist produced by a pressurized spray canister. Frankfurters manufactured without lactate-diacetate by a large commercial manufacturer of franks were sprayed with liquid smoke by using a commercial device as they exited the peeler. When inoculated at three different levels (101,102, and 103 CFU) with a four-strain cocktail of L. monocytogenes and stored at 6°C (43°F), the smoke-treated samples again demonstrated effective control of L. monocytogenes relative to untreated control samples. Frankfurters produced in-house without lactate-diacetate and treated while still in the casing also showed suppression of Listeria compared with controls. The data show that surface application of liquid smoke extracts by dipping or spraying may inhibit the growth of L. monocytogenes on frankfurters during shelf life and should facilitate a claim as an alternative 2, and possibly alternative 1, process for (U.S. Food and Drug Administration) hazard analysis and critical control point purposes.

scholarly journals Good Manufacturing Practices, Hazard Analysis and Critical Control Point plan proposal for distilleries of cachaça

2018 ◽  
Vol 75 (5) ◽  
pp. 432-443 ◽  
Author(s):  
Aline Marques Bortoletto ◽  
Giovanni Casagrande Silvello ◽  
André Ricardo Alcarde
Keyword(s):  
Hazard Analysis ◽  
Control Point ◽  
Critical Control Point ◽  
Good Manufacturing Practices
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