Influence of Soft Rot Bacteria on Growth of Listeria monocytogenes on Potato Tuber Slices†

Growth of Listeria monocytogenes on potato tuber slices and its interaction with four representative species of soft rot bacteria (Pseudomonas fluorescens, P. viridiflava, Erwinia carotovora subsp. carotovora, and Xanthomonas campestris) were investigated. When potato tuber slices were inoculated with one of two L. monocytogenes strains (Scott A and ATCC 15313), an increase in numbers of 3 to 4 logs per gram of tissue was observed with samples that were stored at 20°C for 6 days. However, an increase of about 2 logs was observed with samples that were stored at 8°C for 12 days. When potato slices were simultaneously inoculated with L. monocytogenes and one of the four soft rot bacteria, the growth of L. monocytogenes was inhibited in the presence of P. fluorescens or P. viridiflava but was not significantly affected in the presence of E. carotovora or X. campestris. The antagonism of the two pseudomonads to L. monocytogenes was also observed in potato tuber extract and in culture media. Formation of inhibition zones was observed only in iron-deficient media but not in the medium supplemented with FeCl3. In addition, production of fluorescent siderophore (pyoverdin) by these two pseudomonads was demonstrated. L. monocytogenes was unable to colonize macerated plant tissue induced by soft-rotting bacteria 2 days before inoculation of the pathogen. These results indicate that growth of L. monocytogenes on potato tuber slices is differentially affected by soft rot bacteria and that antagonism of fluorescent pseudomonads to L. monocytogenes is possibly caused by the production of iron-chelating siderophore by these pseudomonads.

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Control of Foodborne Pathogens and Soft-Rot Bacteria on Bell Pepper by Three Strains of Bacterial Antagonists†

Journal of Food Protection ◽

10.4315/0362-028x-72.1.85 ◽

2009 ◽

Vol 72 (1) ◽

pp. 85-92 ◽

Cited By ~ 14

Author(s):

CHING-HSING LIAO

Keyword(s):

Foodborne Pathogens ◽

Erwinia Carotovora ◽

Soft Rot ◽

Bell Pepper ◽

Human Pathogens ◽

Iron Deficient ◽

Spoilage Bacteria ◽

Agar Media ◽

Inhibition Zones ◽

Baby Carrots

Forty-two representative strains of native bacteria associated with fresh peeled baby carrots were isolated and characterized. Two of these strains, identified as Pseudomonas fluorescens AG3A (Pf AG3A) and Bacillus YD1, were evaluated in conjunction with another known antagonist, P. fluorescens 2-79 (Pf 2-79), for their potential as biocontrol agents of human pathogens (Listeria monocytogenes, Yersinia enterocolitica, Salmonella enterica, and Escherichia coli O157:H7) and soft-rot bacteria (Erwinia carotovora subsp. carotovora, Pseudomonas marginalis, and Pseudomonas viridiflava). When grown on iron-deficient agar media, all three antagonists produced inhibition zones up to 25 mm in diameter against the growth of human pathogens and soft-rot bacteria. However, when grown on iron-rich agar media, only Pf 2-79 and Bacillus YD1 exhibited antimicrobial activity. Treatment of bell pepper disks with Pf 2-79 or Bacillus YD1 reduced the growth of pathogen by 1.4 to 4.1 log units, depending upon the ratio of the number of antagonist cells to pathogen cells (1:1, 10:1, 100:1, or 1,000:1). The greatest reduction was observed when 10- to 100-fold higher number of antagonists than pathogens was applied. Pf AG3A and Bacillus YD1 reduced the growth of pathogens on pepper disks at 20°C but not at 10°C. However, Pf 2-79 reduced the growth of L. monocytogenes and Y. enterocolitica by up to 4 log units at either 20 or 10°C. Treatment of pepper disks with Pf 2-79 also reduced the incidence of soft rot induced by soft-rot bacteria by 40 to 70%. Pf 2-79 is the most effective of the three antagonists tested for control of spoilage bacteria and human pathogens on bell pepper.

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Kemampuan Bakteri Endofit Akar dan Ubi Kentang untuk Menekan Penyakit Busuk Lunak (Erwinia carotovora pv. carotovora) pada Ubi

Agrikultura ◽

10.24198/agrikultura.v27i3.10880 ◽

2016 ◽

Vol 27 (3) ◽

Author(s):

Noor Istifadah ◽

Muhamad Salman Umar ◽

Sudarjat Sudarjat ◽

Luciana Djaya

Keyword(s):

Biological Control ◽

Potato Tuber ◽

Endophytic Bacteria ◽

Erwinia Carotovora ◽

Soft Rot ◽

Soft Rot Disease ◽

Post Harvest ◽

Rot Disease

ABSTRACTThe abilities of endophytic bacteria from potato roots and tubers to suppress soft rot disease (Erwinia carotovora pv. carotovora) in potato tuberSoft rot disease caused by Erwinia carotovora pv. carotovora is one of limiting factors in cultivation and post harvest of potato. The eco-friendly control measure that can be developed for controlling the diseases is biological control. Microbes that are potential as biological control agents include endophytic bacteria. This paper discussed the results of study examining the potential of endophytic bacteria isolated from roots and tubers of potato to inhibit the growth of E. carotovora pv. carotovora in vitro and suppress soft rot disease in potato tuber. The results showed that among 24 isolates examined, four isolates of endophytic bacteria (one isolate from potato tuber and three isolates from potato roots) inhibited the growth of E. carotovora pv. carotovora in vitro with inhibition zone 3.5-6.8 mm. In the in vivo test, the isolates inhibited the soft rot disease in potato tuber by 71.5-86.4%. The isolate that tended to show relatively better inhibition in vitro and in vivo was isolate from potato tuber which is CK U3 (Lysinibacillus sp.)Keywords: Biological control, Endophytic bacteria, Post-harvest, Potato, Soft rot diseaseABSTRAKPenyakit busuk lunak yang disebabkan bakteri Erwinia carotovora pv. carotovora, merupakan salah satu kendala dalam budidaya dan pascapanen kentang. Cara pengendalian ramah lingkungan yang dapat dikembangkan untuk menekan penyakit tersebut adalah pengendalian biologi. Kelompok mikroba yang berpotensi sebagi agens pengendali biologi adalah bakteri endofit. Artikel ini mendiskusikan potensi isolat bakteri endofit yang berasal dari ubi dan akar kentang untuk menghambat pertumbuhan bakteri E. carotovora pv. carotovora secara in vitro dan menekan perkembangan penyakit busuk lunak pada ubi kentang. Hasil percobaan menunjukkan bahwa diantara 24 isolat bakteri yang diuji, terdapat empat isolat bakteri endofit (satu isolat dari ubi kentang dan tiga isolat dari akar kentang) yang dapat menghambat pertumbuhan bakteri E. carotovora pv. carotovora secara in vitro dengan zona penghambatan sebesar 3,5-6,8 mm. Pada pengujian secara in vivo, isolat-isolat tersebut dapat menekan perkembangan penyakit busuk lunak pada ubi kentang sebesar 71,5-86,4%. Isolat yang cenderung menunjukkan penghambatan relatif lebih baik secara in vitro dan in vivo adalah isolat bakteri endofit asal ubi kentang yaitu isolat CK U3 (Lysinibacillus sp.).Kata Kunci: Pengendalian biologi, Bakteri endofit, Pascapanen, Kentang, Penyakit busuk basah

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Efficacy of Mahogany Bark Aqueous Extracts and Exposure to Solar Heat for Treatment of Potato Tuber Soft Rot Caused by Erwinia Carotovora Ssp. Carotovora

Journal of Plant Protection Research ◽

10.2478/v10045-010-0066-5 ◽

2010 ◽

Vol 50 (3) ◽

pp. 393-397

Author(s):

Bulus Bdliya ◽

Peter Abraham

Keyword(s):

Potato Tuber ◽

Plant Extract ◽

Erwinia Carotovora ◽

Soft Rot ◽

Bark Extract ◽

Control Group ◽

Aqueous Extracts ◽

Solar Heat ◽

Severity Of The Disease ◽

Tuber Treatment

Efficacy of Mahogany Bark Aqueous Extracts and Exposure to Solar Heat for Treatment of Potato Tuber Soft Rot Caused byErwinia CarotovoraSsp.CarotovoraThe efficacy of combining tuber treatment with mahogany bark aqueous extracts and exposure to solar heat for the control of potato tuber soft rot was investigated. Artificially inoculated potato tubers were treated with mahogany bark aqueous extracts and exposed to solar heat for zero, one, two and three hours. The results showed that tuber treatment with the plant extract followed by exposure to solar heat significantly reduced the incidence and severity of tuber soft rot compared to the control group. However, the highest reduction in the incidence and severity of the disease was recorded on tubers treated with the plant extract and incubated immediately after treatment (no exposure to solar heat). This suggests that the plant extract is more effective at lower than higher temperatures. Potato tuber losses due to soft rot could therefore be managed by tuber treatment with mahogany bark extract and no exposure to solar heat.

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The Potential Applications of Bacillus sp. and Pseudomonas sp. Strains with Antimicrobial Activity against Phytopathogens, in Waste Oils and the Bioremediation of Hydrocarbons

Catalysts ◽

10.3390/catal9110959 ◽

2019 ◽

Vol 9 (11) ◽

pp. 959 ◽

Cited By ~ 2

Author(s):

Mariana-Gratiela Soare (Vladu) ◽

Elena Simina Lakatos ◽

Nicoleta Ene ◽

Nereida Malo (Dalanaj) ◽

Ovidiu Popa ◽

...

Keyword(s):

Antimicrobial Activity ◽

Xanthomonas Campestris ◽

Selection Process ◽

Erwinia Carotovora ◽

Luria Bertani ◽

Bacterial Strains ◽

Plant Materials ◽

Waste Oils ◽

Potential Applications ◽

Inhibition Zones

Biodegradation is one of the primary mechanisms for the elimination of petroleum and other hydrocarbon pollutants from the environment. This study presents the results obtained with two newly isolated microorganisms and their potential applications in bioremediation, agriculture, and industrial fields. Twenty-five strains of microorganisms were isolated from plant materials and were subject to a selection process on the basis of antimicrobial activity. Two bacterial strains, respectively Bacillus mycoides (Bm) and Pseudomonas putida (B1), were selected for further experiments, based on the largest inhibition zones against the phytopathogens Erwinia carotovora and Xanthomonas campestris. The production of biosurfactants and enzymes was evaluated in specific media. In order to assess the production of biosurfactants, submerged bioprocesses were carried out on Yeast Malt Peptone Glucose (YMPG), M44, Luria-Bertani (LB), and King B media (KB); the supernatants were used to form emulsions with heptane, octane, and sunflower oil, and the emulsifying indices were determined.

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Xanthomonas campestris pv. campestris (black rot).

10.1079/cpc.56919.20210100335 ◽

2021 ◽

Author(s):

Carmen Nieves Mortensen

Keyword(s):

New Zealand ◽

Xanthomonas Campestris ◽

Central And Eastern Europe ◽

Erwinia Carotovora ◽

Soft Rot ◽

Crop Damage ◽

Northern Europe ◽

Black Rot ◽

Opportunistic Pathogens ◽

Xanthomonas Campestris Pv Campestris

Abstract Black rot is considered the most important worldwide disease of crucifers. The disease is known to exist in the cool coastal climates of northern Europe and North America but was seldom a problem there until the 1990s. Its potential for crop damage is also considered low in New Zealand, and parts of Australia. In many regions of Central and Eastern Europe, Central Asia (Kazakhstan), China, tropical and subtropical regions of Asia, Africa and South America where brassicas are common and cultivated without crop rotation, black rot is always present. Seed production in those regions is commonly associated with high levels of seedborne Xcc (Williams, 1980). Even minor, visually undetectable development of black rot may considerably increase damage to plants by soft rot caused by Erwinia carotovora, Pseudomonas spp. and other opportunistic pathogens (Djalilov et al., 1989).

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The Role of Polyphenol Oxidase and Peroxidase in Potato Tuber Resistance to Soft Rot Caused by Erwinia carotovora

Journal of Phytopathology ◽

10.1111/j.1439-0434.1992.tb01314.x ◽

1992 ◽

Vol 136 (4) ◽

pp. 319-328 ◽

Cited By ~ 14

Author(s):

E. Łojkowska ◽

M. Hołubowska

Keyword(s):

Potato Tuber ◽

Polyphenol Oxidase ◽

Erwinia Carotovora ◽

Soft Rot ◽

Tuber Resistance

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Screening of Antagonistic Bacterium to Control Konjac Soft Rot

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.726-731.4427 ◽

2013 ◽

Vol 726-731 ◽

pp. 4427-4430

Author(s):

Guo Hua Chen

Keyword(s):

Bacterial Strain ◽

Bacterial Pathogen ◽

Erwinia Carotovora ◽

Soft Rot ◽

Field Trials ◽

Antagonistic Effect ◽

Soil Samples ◽

Plate Method ◽

Antagonistic Bacterium

Konjac soft rot is a bottleneck limiting konjac yield caused by bacterial strain of Erwinia carotovora var. carotovora. In order to control konjac soft rot, soil samples were collected, and each sample was spread on surface of a plate seeded with E. carotovora var. carotovora in advance. Strains expressing antagonistic activities were selected and then isolated with streak plate method. One bacterial strain (named Z10) was obtained from soil by this method. In field trials, strain Z10 still showed antagonistic effect against the bacterial pathogen.

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