scholarly journals The Potential Applications of Bacillus sp. and Pseudomonas sp. Strains with Antimicrobial Activity against Phytopathogens, in Waste Oils and the Bioremediation of Hydrocarbons

Catalysts ◽  
2019 ◽  
Vol 9 (11) ◽  
pp. 959 ◽  
Author(s):  
Mariana-Gratiela Soare (Vladu) ◽  
Elena Simina Lakatos ◽  
Nicoleta Ene ◽  
Nereida Malo (Dalanaj) ◽  
Ovidiu Popa ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Xanthomonas Campestris ◽  
Selection Process ◽  
Erwinia Carotovora ◽  
Luria Bertani ◽  
Bacterial Strains ◽  
Plant Materials ◽  
Waste Oils ◽  
Potential Applications ◽  
Inhibition Zones

Biodegradation is one of the primary mechanisms for the elimination of petroleum and other hydrocarbon pollutants from the environment. This study presents the results obtained with two newly isolated microorganisms and their potential applications in bioremediation, agriculture, and industrial fields. Twenty-five strains of microorganisms were isolated from plant materials and were subject to a selection process on the basis of antimicrobial activity. Two bacterial strains, respectively Bacillus mycoides (Bm) and Pseudomonas putida (B1), were selected for further experiments, based on the largest inhibition zones against the phytopathogens Erwinia carotovora and Xanthomonas campestris. The production of biosurfactants and enzymes was evaluated in specific media. In order to assess the production of biosurfactants, submerged bioprocesses were carried out on Yeast Malt Peptone Glucose (YMPG), M44, Luria-Bertani (LB), and King B media (KB); the supernatants were used to form emulsions with heptane, octane, and sunflower oil, and the emulsifying indices were determined.

Influence of Soft Rot Bacteria on Growth of Listeria monocytogenes on Potato Tuber Slices†

1999 ◽  
Vol 62 (4) ◽  
pp. 343-348 ◽  
Author(s):  
CHING-HSING LIAO ◽  
GERALD M. SAPERS
Keyword(s):  
Listeria Monocytogenes ◽  
Potato Tuber ◽  
Xanthomonas Campestris ◽  
Culture Media ◽  
Erwinia Carotovora ◽  
Soft Rot ◽  
Representative Species ◽  
Iron Deficient ◽  
Inhibition Zones ◽  
Tuber Extract

Growth of Listeria monocytogenes on potato tuber slices and its interaction with four representative species of soft rot bacteria (Pseudomonas fluorescens, P. viridiflava, Erwinia carotovora subsp. carotovora, and Xanthomonas campestris) were investigated. When potato tuber slices were inoculated with one of two L. monocytogenes strains (Scott A and ATCC 15313), an increase in numbers of 3 to 4 logs per gram of tissue was observed with samples that were stored at 20°C for 6 days. However, an increase of about 2 logs was observed with samples that were stored at 8°C for 12 days. When potato slices were simultaneously inoculated with L. monocytogenes and one of the four soft rot bacteria, the growth of L. monocytogenes was inhibited in the presence of P. fluorescens or P. viridiflava but was not significantly affected in the presence of E. carotovora or X. campestris. The antagonism of the two pseudomonads to L. monocytogenes was also observed in potato tuber extract and in culture media. Formation of inhibition zones was observed only in iron-deficient media but not in the medium supplemented with FeCl3. In addition, production of fluorescent siderophore (pyoverdin) by these two pseudomonads was demonstrated. L. monocytogenes was unable to colonize macerated plant tissue induced by soft-rotting bacteria 2 days before inoculation of the pathogen. These results indicate that growth of L. monocytogenes on potato tuber slices is differentially affected by soft rot bacteria and that antagonism of fluorescent pseudomonads to L. monocytogenes is possibly caused by the production of iron-chelating siderophore by these pseudomonads.

scholarly journals Novel Substituted Indazoles towards Potential Antimicrobial Agents

2021 ◽  
Vol 37 (2) ◽  
pp. 508-512
Author(s):  
Jaganmohana Rao Saketi ◽  
S N Murthy Boddapati ◽  
Raghuram M ◽  
Geetha Bhavani Koduru ◽  
Haribabu Bollikolla
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Xanthomonas Campestris ◽  
Antimicrobial Agents ◽  
Evaluation Studies ◽  
Antimicrobial Activities ◽  
Biological Evaluation ◽  
Fungal Strain ◽  
Bacterial Strains

The in vitroantimicrobial properties of a series of N-methyl-3-aryl indazoles (5a-5j) were screened. In this present work, we describe our efforts towards the development of potent antimicrobial activity of synthesized indazole derivatives. The antimicrobial activities of the prepared compounds were investigated against four bacterial strains: Xanthomonas campestris, Escherichia coli, Bacillus cereus, Bacillus megaterium, and a fungal strain Candida albicans. The biological evaluation studies of these indazole derivatives revealed that some of these tested compounds have shown moderate to goodin vitroantimicrobial activities.

scholarly journals Phenolic Composition, Antioxidant and Antimicrobial Activity of Cotoneaster Medik. Species from Bosnia and Herzegovina

2020 ◽  
Keyword(s):  
Antimicrobial Activity ◽  
Leaf Extract ◽  
Acetylcholinesterase Inhibition ◽  
Bacterial Strains ◽  
Methanolic Extracts ◽  
Microbial Strains ◽  
Inhibition Zones ◽  
Antioxidant And Antimicrobial Activity ◽  
Antibacterial And Antifungal ◽  
Ornamental Species

Although the genus Cotoneaster Medik. includes mainly ornamental species, there are some data regarding its biological activity. The purpose of this study was to analyze the content of phenolic compounds, acetylcholinesterase inhibition, antioxidant and antimicrobial activity of methanolic extracts of leaf and bark of C. integerrimus Medik., C. tomentosus (Aiton) Lindl. and C. horizontalis Decne. The C. tomentosus leaf extract exhibited the highest content of total phenols (135.86 mg GAE/g) and flavonoids (18.17 mgQE/g), and also the most potent antioxidant activity against nonbiogenic free radicals, while the highest inhibition of acetylcholinesterase had the leaf extract of C. horizontalis (IC50 0.34 mg/mL). All extracts showed a significant level of antibacterial and antifungal activity against tested microbial strains. The largest inhibition zones were observed against Candida albicans treated with C. integerrimus leaf extract (30.50±0.50 mm). Furthermore, C. integerrimus extract was the most effective in the majority of bacterial strains tested. The results indicated that methanolic extracts of the investigated Cotoneaster species have promising bioactive and therapeutic potentials

scholarly journals STUDY OF BIOCHEMICAL COMPOUNDS AND ANTIMICROBIAL ACTIVITY OF PLEUROTUS INDICA MUSHROOM

2015 ◽  
Vol 2 (2) ◽  
pp. 100-103
Author(s):  
S Pria ◽  
O. N. Shanmugapriya
Keyword(s):  
Antimicrobial Activity ◽  
Phenolic Compounds ◽  
Value Added ◽  
Antibacterial Activities ◽  
Bacterial Strains ◽  
Total Carotenoids ◽  
Nutritional Values ◽  
Biochemical Compounds ◽  
Inhibition Zones ◽  
Cultivated Mushrooms

In this study, the nutritional values of dietary mushrooms-Pleurotus indica that are very popular among the cultivated mushrooms in India have been determined. P.indica is widely cultivated as it is rich in nutrients and its component. P.indica is useful in biomass production in commercial level as it contains proteins and minerals. In this study the value added biochemical compounds in P.indica were analyzed from cultures. The amount of total carotenoids, protein, vitamin, pigments and phenolic compounds were examined. P.indica extracts possessed great potential antibacterial activities against four bacterial strains with inhibition zones ranged 8-15mm and MICs values ranges from 25-40É¥g/mL. Thus, it could be suggested that the P.indica is useful biosystem for production of biochemical compounds possess an antimicrobial principles.

scholarly journals PHYTOCHEMICAL SCREENING OF ARTOCARPUS HIRSUTUS AND ITS ANTIMICROBIAL POTENTIAL

2017 ◽  
Vol 10 (6) ◽  
pp. 298 ◽  
Author(s):  
Shanmugapriya K ◽  
Akanya S ◽  
Anderson Clinton B ◽  
Berril Nanzy S ◽  
Prakash Pr ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Phytochemical Screening ◽  
Bacterial Strains ◽  
Zone Of Inhibition ◽  
Fruit Extract ◽  
Aspergillus Tamarii ◽  
Plant Materials ◽  
Bioactive Phytochemicals ◽  
Phytochemical Components

Objective: In the present study, the effort was aimed to evaluate the phytochemical components, antioxidant activity, and antimicrobial activity of Artocarpus hirsutus fruit and leaf. Methods: Each plant materials were extracted with two suitable solvents such as acetone and ethanol. Then, the extracts were tested for the presence of active phytoconstituents and antimicrobial activity. Then, it was subjected to antimicrobial activity against fungal and bacterial strains. Results: The present study suggested that the selected plant extracts of A. hirsutus exhibit major phytoconstituents, such as alkaloids, glycosides, terpenoids, phenols, and saponins. Furthermore, the results showed that acetone fruit extract of A. hirsutus and ethanolic fruit extract of A. hirsutus exhibited significant antimicrobial activity with a maximum zone of inhibition against Staphylococcus aureus and Aspergillus tamarii. Conclusion: The present study also exhibited remarkable antimicrobial activity against all the test microorganisms due to the presence of major bioactive phytochemicals

scholarly journals Effects of Natural Antimicrobials on Xanthomonas Strains Growth

Horticulturae ◽  
2021 ◽  
Vol 8 (1) ◽  
pp. 7
Author(s):  
Irena Mačionienė ◽  
Dovilė Čepukoit ◽  
Joana Šalomskienė ◽  
Darius Černauskas ◽  
Daiva Burokienė ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oils ◽  
Lactobacillus Helveticus ◽  
Mentha Piperita ◽  
Diffusion Method ◽  
Thymus Vulgaris ◽  
Aqueous Extracts ◽  
Bacterial Strains ◽  
Natural Antimicrobials ◽  
Inhibition Zones

The aim of this work was to investigate the most promising natural antimicrobials effective for the growth suppression of Xanthomonas spp. bacteria. The research objects were Xanthomonas spp. strains isolated from tubers and stem of plants growing in Lithuania: Xanthomonas translucens NRCIB X6, X. arboricola NRCIB X7, NRCIB X8, NRCIB X9, and NRCIB X10; the supernatants of lactic acid bacteria Lactococcus lactis strains 140/2, 57, and 768/5, Lactobacillus helveticus strains 14, 148/3, R, and 3, Lb. reuteri 3 and 7, Streptococcus thermophilus 43, Enterococcus faecium 59-30 and 41-2; endophytic bacterial strains Bacillus, Pseudomonas, and Paenibacillus spp.; and essential oils of lavender (Lavandula angustifolia), grapefruit (Citrus paradisi), pine (Pinus sylvestris), thyme (Thymus vulgaris), rosemary (Rosmarinus officinalis), peppermint (Mentha piperita), lemon (Citrus limetta), aqueous extracts of blueberries (Vaccinium myrtillus), and cranberries (Vaccinium vitis-idaea). The antimicrobial activity of tested substances was determined by agar diffusion method. Supernatants of Lb. reuteri strain 7 and Lb. helveticus strains 14, R, 3, and 148/3 were found to have a high antimicrobial activity against Xanthomonas spp. bacteria strains when compared to the positive control—1.0% copper sulfate (diameter of inhibition zones was 28.8 ± 0.7 mm). The diameter of inhibition zones of supernatants ranged from 23.3 ± 0.6 mm to 32.0 ± 0.1 mm. Thyme (2.0%) and lavender (2.0%) essential oils inhibited the growth of Xanthomonas spp. strains. The diameter of the inhibition zones was from 14.7 ± 0.8 mm to 22.8 ± 0.9 mm. The aqueous extracts of blueberries had a weak antimicrobial activity. The diameter of inhibition zones ranged from 11.0 ± 0.2 mm to 13.0 ± 0.2 mm.

scholarly journals Antimicrobial Activity of Chloroform and Methanol Extract of Lennea coromandelica Merr

2015 ◽  
Vol 45 ◽  
pp. 67-74 ◽  
Author(s):  
P. Vedhanarayanan ◽  
T. Vaithiyanathan ◽  
P. Sundaramoorthy
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Diffusion Method ◽  
Bacterial Strains ◽  
Methanol Extracts ◽  
Gram Positive Bacteria ◽  
Zones Of Inhibition ◽  
Inhibition Zones ◽  
Bacterial Concentrations

The antimicrobial activity of chloroform and methanol extracts of Lennea coromandelica were screened for their was studied against gram positive bacteria strains Staphylococcus aureus,Streptococcus pyogenes and Bacillus subtilis gram negative bacteria strains Pseudomonas aeruginosa, Klebsiella pneumoniae and Escherichia coli and fungal strains such as Candida albicans, C. parapsilosis, C. krusei and C. glabrata using disc diffusion method, determination of Minimum Inhibitory Concentrations (MIC), Minimum Bacterial Concentrations (MBC) and Minimum Fungicidal Concentrations (MFC). The antimicrobial activity for different concentrations like 250 μg, 500 μg and 1000 μg of different solvent extracts of Lannea cormandelica. bacterial strains and recorded in highest mean zones of inhibition ranged from 19.6 mm and Candidal strains and the exhibited the highest mean zones of inhibition ranged from 10.6 mm. Methanol extracts showed the best results as inhibition zones against tested organisms. Results showed also that, the greatest effect was towards Staphylococcus aureus and the lowest was against Candida krusei. The present study reported the great effect of Lannea cormandelica extracts against some of most important pathogens.

SYNTHESIS, CHARACTERIZATION AND BIOLOGICAL EVALUATION OF 3-FORMYL INDOLE BASED SCHIFF BASES

2018 ◽  
Vol 7 (6) ◽  
Author(s):  
Singh Gurvinder ◽  
Singh Prabhsimran ◽  
Dhawan R. K.
Keyword(s):  
Antimicrobial Activity ◽  
Spectral Analysis ◽  
Schiff Bases ◽  
Antimicrobial Agents ◽  
Biological Evaluation ◽  
Fungal Strain ◽  
Bacterial Strains ◽  
Standard Drug ◽  
Gram Negative ◽  
E Coli

In order to develop new antimicrobial agents, a series of 3-formyl indole based Schiff bases were synthesized by reacting 3-formyl indole(indole-3-carboxaldehyde) with substituted aniline taking ethanol as solvent. The reaction was carried in the presence of small amount of p-toluene sulphonic acid as catalyst.All the synthesized compounds were characterized by IR, 1H-NMR spectral analysis. All the synthesized compounds were evaluated for antimicrobial activity against two gram positive bacterial strains (B. subtilisand S. aureus) and two gram negative bacterial strains (P. aeruginosaand E. coli) and one fungal strain (C. albicans). All the synthesized compounds were found to have moderate to good antimicrobial activity. The  standard drug amoxicillin, fluconazole were used for antimicrobial activity. Among the synthesized compounds, the maximum antimicrobial activity was shown by compounds GS04, GS07, GS08 and GS10.

Antimicrobial Activity of Iron-Halide Complexes Against Gram-Positive and Gram-Negative Bacteria

2020 ◽  
Author(s):  
Nusrat Abedin ◽  
Abdullah Hamed A Alshehri ◽  
Ali M A Almughrbi ◽  
Olivia Moore ◽  
Sheikh Alyza ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antimicrobial Resistance ◽  
Extracellular Dna ◽  
Antibiofilm Activity ◽  
Gram Negative Bacteria ◽  
Bacterial Strains ◽  
Gram Positive ◽  
Gram Negative ◽  
Antimicrobial Substances ◽  
Mammalian Cell Lines

Antimicrobial resistance (AMR) has become one of the more serious threats to the global health. The emergence of bacteria resistant to antimicrobial substances decreases the potencies of current antibiotics. Consequently, there is an urgent and growing need for the developing of new classes of antibiotics. Three prepared novel iron complexes have a broad-spectrum antimicrobial activity with minimum bactericidal concentration (MBC) values ranging from 3.5 to 10 mM and 3.5 to 40 mM against Gram-positive and Gram-negative bacteria with antimicrobial resistance phenotype, respectively. Time-kill studies and quantification of the extracellular DNA confirmed the bacteriolytic mode of action of the iron-halide compounds. Additionally, the novel complexes showed significant antibiofilm activity against the tested pathogenic bacterial strains at concentrations lower than the MBC. The cytotoxic effect of the complexes on different mammalian cell lines show sub-cytotoxic values at concentrations lower than the minimum bactericidal concentrations.

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