Additive Approach for Inactivation of Escherichia coli O157:H7, Salmonella, and Shigella spp. on Contaminated Fresh Fruits and Vegetables Using Bacteriophage Cocktail and Produce Wash

2013 ◽  
Vol 76 (8) ◽  
pp. 1336-1341 ◽  
Author(s):  
JOSHUA P. MAGNONE ◽  
PATRICK J. MAREK ◽  
ALEXANDER SULAKVELIDZE ◽  
ANDRE G. SENECAL
Keyword(s):  
Escherichia Coli ◽  
Effective Treatment ◽  
Levulinic Acid ◽  
Fruits And Vegetables ◽  
Potable Water ◽  
Organic Content ◽  
Escherichia Coli O157 ◽  
Lytic Bacteriophage ◽  
Shigella Spp ◽  
Acid Produce

The incidence of foodborne outbreaks involving fresh produce is of worldwide concern. Lytic bacteriophage cocktails and a levulinic acid produce wash were investigated for their effectiveness against the foodborne pathogens Escherichia coli O157:H7, Shigella spp., and Salmonella on broccoli, cantaloupe, and strawberries. Inoculated samples were treated with bacteriophage cocktails (BC) before storage at 10°C for 24 h, a levulinic acid produce wash (PW) after storage at 10°C for 24 h, or a combination of the washes (BCPW) before and after storage. All three treatments were compared against a 200-ppm free available chlorine wash. Wash solutions were prepared using potable water and water with an increased organic content of 2.5 g/liter total dissolved solids and total organic carbon. BCPW was the most effective treatment, producing the highest log reductions in the pathogens. Produce treated with BCPW in potable water with a PW exposure time of 5 min resulted in the highest reduction of each pathogen for all samples tested. The type of produce and wash solution had significant effects on the efficacy of the individual treatments. The chlorine wash in water with higher organic content was the least effective treatment tested. An additive effect of BCPW was seen in water with higher organic content, resulting in greater than 4.0-log reductions in pathogens. Our findings indicate that the combination of antimicrobial BC with a commercial produce wash is a very effective method for treating produce contaminated with E. coli O157:H7, Shigella spp., and Salmonella even in the presence of high loads of organic matter.

Microbiological Quality of Retail Imported Unprepared Whole Lettuces: A PHLS Food Working Group Study

1999 ◽  
Vol 62 (4) ◽  
pp. 325-328 ◽  
Author(s):  
C. LITTLE ◽  
D. ROBERTS ◽  
E. YOUNGS ◽  
J. de LOUVOIS
Keyword(s):  
Escherichia Coli ◽  
Listeria Monocytogenes ◽  
Microbiological Quality ◽  
Escherichia Coli O157 ◽  
Salmonella Spp ◽  
Shigella Spp ◽  
Natural Microflora ◽  
Production Practices ◽  
Campylobacter Spp

A study of imported unprepared whole lettuces sampled from supermarkets, greengrocers, shops, and market stalls found that all were of acceptable microbiological quality. Twenty-seven out of 151 (18%) imported lettuce samples had Enterobacteriaceae levels of 104 CFU/g or more. However, these bacteria that constitute part of the natural microflora of unprepared vegetables may also be derived from the soil and/or by poor handling. The pathogens, Salmonella spp., Shigella spp., Campylobacter spp., Escherichia coli O157:H7, Vibrio cholerae, Listeria monocytogenes, and also Escherichia coli, an indicator of fecal contamination, were not detected in any imported lettuces, indicating that hygiene, harvesting, and production practices were good. Imported lettuces with Enterobacteriaceae levels of 104 CFU/g or more varied with type of retail premises and the temperature at which the lettuces were displayed. Samples from greengrocers, shops, and market stalls were more likely to contain Enterobacteriaceae at levels in excess of 104 CFU/g than those from supermarkets.

A capacitive DNA sensor for sensitive detection of Escherichia coli O157:H7 in potable water based on the z3276 genetic marker: fabrication and analytical performance

The Analyst ◽  
2020 ◽  
Vol 145 (6) ◽  
pp. 2267-2278 ◽  
Author(s):  
Rehan Deshmukh ◽  
Arun Kumar Prusty ◽  
Utpal Roy ◽  
Sunil Bhand
Keyword(s):  
Escherichia Coli ◽  
Genetic Marker ◽  
Potable Water ◽  
Analytical Performance ◽  
Sensitive Detection ◽  
Escherichia Coli O157 ◽  
Dna Sensor ◽  
Label Free ◽  
Dna Sensing ◽  
Water Based

We report a label-free biosensor for the detection of Escherichia coli O157:H7 ATCC 43895 in potable water using a newly designed DNA sensing probe targeting the z3276 genetic marker.

Evaluation of Chemical and Microbiological Contaminants in Fresh Fruits and Vegetables from Peasant Markets in Cundinamarca, Colombia

2020 ◽  
Vol 83 (10) ◽  
pp. 1726-1737
Author(s):  
MANUEL PATIÑO ◽  
MARÍA FERNANDA VALENCIA-GUERRERO ◽  
EDWIN SAMIR BARBOSA-ÁNGEL ◽  
MARÍA JOSÉ MARTÍNEZ-CORDÓN ◽  
PILAR DONADO-GODOY
Keyword(s):  
Escherichia Coli ◽  
Heavy Metals ◽  
Listeria Monocytogenes ◽  
Fruits And Vegetables ◽  
Integrated Management ◽  
Management Strategies ◽  
Escherichia Coli O157 ◽  
Pathogenic Microorganisms ◽  
Maximum Residue Limit ◽  
Integrated Crop Management

ABSTRACT In this study, chemical and microbiological contaminants were detected in fresh fruits and vegetables in peasant markets in the Department of Cundinamarca to assess food safety. At least one pesticide was detected in 63% of the samples evaluated (n = 100, including fruits and vegetables), and in 41% of these, pesticides exceeded the maximum residue limit. Within the pesticides found, 60% do not use a registry from the Instituto Colombiano Agropecuario to be applied on the crops where they were detected and are included in the toxicological categories “highly toxic” (category IB) and “moderately toxic” (category II). Heavy metals were found in 45% of the samples, with cadmium (38%) and lead (14%) being the most frequent. The pathogenic microorganisms Escherichia coli O157:H7, Listeria monocytogenes, Salmonella, and Campylobacter sp. were detected in 4, 2, 1, and 0% of the samples, respectively. Factors concerning integrated crop management, contaminated inputs with chemicals and microorganisms, and the lack of hygiene in the postharvest stage favor the presence of pesticide residues, heavy metals, and the appearance of pathogenic microorganisms. The results suggest the need to strengthen the implementation of integrated management strategies that guarantee the safety of fresh fruits and vegetables in the country. HIGHLIGHTS

scholarly journals Isolation and Identification of Escherichia coli O157:H7 Lytic Bacteriophage from Environment Sewage

2021 ◽  
Vol 2021 ◽  
pp. 1-11
Author(s):  
Tessa Sjahriani ◽  
Eddy Bagus Wasito ◽  
Wiwiek Tyasningsih
Keyword(s):  
Escherichia Coli ◽  
Host Specificity ◽  
Pathogenic Bacteria ◽  
Statistical Tests ◽  
Plaque Morphology ◽  
Escherichia Coli O157 ◽  
Lytic Bacteriophage ◽  
Foodborne Disease ◽  
Isolation And Identification ◽  
E Coli

Escherichia coli O157:H7 is one of the pathogenic bacteria causing foodborne disease. The use of lytic bacteriophages can be a good solution to overcome the disease. This study is aimed at isolating lytic bacteriophages from environmental sewage with E. coli O157:H7 bacterial cells. The sample used in this study was eight bacteriophages, and the technique used in identifying E. coli O157:H7 carriers of the stx1 and stx2 genes was PCR. The double layer plaque technique was used to classify bacteriophages. Plaque morphology, host specificity, and electron micrograph were used to identify the bacteriophages. The result obtained plaque morphology as a clear zone with the largest diameter size of 3.5 mm. Lytic bacteriophage could infect E. coli O157:H7 at the highest titer of 10 × 10 8   PFU / mL . Bacteriophages have been identified as Siphoviridae and Myoviridae. Phage 3, phage 4, and phage 8 could infect Atypical Diarrheagenic E. coli 1 (aDEC1) due to their host specificity. The Friedman statistical tests indicate that lytic bacteriophage can significantly lyse E. coli O157:H7 ( p = 0.012 ). The lysis of E. coli O157:H7 by phage 1, phage 2, phage 3, and phage 5 bacteriophages was statistically significant, according to Conover’s posthoc test ( p < 0.05 ). The conclusion obtained from this study is that lytic bacteriophages from environmental sewage could lyse E. coli O157:H7. Therefore, it could be an alternative biocontrol agent against E. coli O157:H7 that contaminates food causing foodborne disease.

Use of Bacteriophages to Control Escherichia coli O157:H7 in Domestic Ruminants, Meat Products, and Fruits and Vegetables

2017 ◽  
Vol 14 (9) ◽  
pp. 483-493 ◽  
Author(s):  
Lili Wang ◽  
Kunli Qu ◽  
Xiaoyu Li ◽  
Zhenhui Cao ◽  
Xitao Wang ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Fruits And Vegetables ◽  
Meat Products ◽  
Escherichia Coli O157 ◽  
Domestic Ruminants

Reduction of Escherichia coli O157:H7 on Produce by Use of Electrolyzed Water under Simulated Food Service Operation Conditions

2009 ◽  
Vol 72 (9) ◽  
pp. 1854-1861 ◽  
Author(s):  
PHILIPUS PANGLOLI ◽  
YEN-CON HUNG ◽  
LARRY R. BEUCHAT ◽  
C. HAROLD KING ◽  
ZHI-HUI ZHAO
Keyword(s):  
Escherichia Coli ◽  
Foodborne Pathogens ◽  
Fruits And Vegetables ◽  
Tap Water ◽  
Food Service ◽  
Escherichia Coli O157 ◽  
Electrolyzed Water ◽  
E Coli ◽  
Operation Conditions ◽  
Iceberg Lettuce

Treatment of fresh fruits and vegetables with electrolyzed water (EW) has been shown to kill or reduce foodborne pathogens. We evaluated the efficacy of EW in killing Escherichia coli O157:H7 on iceberg lettuce, cabbage, lemons, and tomatoes by using washing and/or chilling treatments simulating those followed in some food service kitchens. Greatest reduction levels on lettuce were achieved by sequentially washing with 14-A (amperage) acidic EW (AcEW) for 15 or 30 s followed by chilling in 16-A AcEW for 15 min. This procedure reduced the pathogen by 2.8 and 3.0 log CFU per leaf, respectively, whereas washing and chilling with tap water reduced the pathogen by 1.9 and 2.4 log CFU per leaf. Washing cabbage leaves for 15 or 30 s with tap water or 14-A AcEW reduced the pathogen by 2.0 and 3.0 log CFU per leaf and 2.5 to 3.0 log CFU per leaf, respectively. The pathogen was reduced by 4.7 log CFU per lemon by washing with 14-A AcEW and 4.1 and 4.5 log CFU per lemon by washing with tap water for 15 or 30 s. A reduction of 5.3 log CFU per lemon was achieved by washing with 14-A alkaline EW for 15 s prior to washing with 14-A AcEW for 15 s. Washing tomatoes with tap water or 14-A AcEW for 15 s reduced the pathogen by 6.4 and 7.9 log CFU per tomato, respectively. Application of AcEW using procedures mimicking food service operations should help minimize cross-contamination and reduce the risk of E. coli O157:H7 being present on produce at the time of consumption.

scholarly journals Hoàn thiện quy trình phát hiện đồng thời 14 vi khuẩn gây bệnh đường ruột bằng kĩ thuật PCR-Reverse Dot Blot (PCR-RDB)

2020 ◽  
Vol 13 (1) ◽  
pp. 66-80
Author(s):  
Hồ Thị Thanh Thủy ◽  
Nguyễn Văn Trường ◽  
Nguyễn Bảo Toàn ◽  
Lao Đức Thuận ◽  
Trương Kim Phượng ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Campylobacter Jejuni ◽  
Yersinia Enterocolitica ◽  
Vibrio Parahaemolyticus ◽  
Clostridium Botulinum ◽  
Escherichia Coli O157 ◽  
Dot Blot ◽  
Salmonella Spp ◽  
Shigella Spp ◽  
Reverse Dot Blot

Ngộ độc thực phẩm, với một trong những nguyên nhân chính do nhiễm khuẩn vẫn luôn là mối lo ngại, mang tính toàn cầu, được Tổ chức Sức khỏe Thế giới rất quan tâm. Việc xác định chính xác đối tượng vi khuẩn nhiễm vẫn luôn là một nhu cầu cấp thiết của các labo lâm sàng. Nghiên cứu trước đây của chúng tôi đã được công bố với việc thành công bước đầu trong việc xây dựng một quy trình dựa trên kĩ thuật PCR-Reverse Dot Blot (PCR-RDB) nhằm xác định đồng thời 12 vi khuẩn gây bệnh đường ruột ch yếu, bao gồm Bacillus cereus, Clostridium botulinum, Clostridium perfringen, Staphylococcus aureus, Listeria monocytogenes, Escherichia coli O157:H7, Salmonella spp., Shigella spp.., Vibrio cholerae, Vibrio parahaemolyticus, Yersinia enterocolitica và Brucella spp. Chúng tôi tiếp tục phát triển nghiên cứu này nhằm hoàn thiện quy trình PCR-RDB bằng việc thiết kế bổ sung trên màng các loại mẫu dò nhằm làm chứng dương, chứng âm, chứng màu và chứng kiểm tra tín hiệu nền. Bên cạnh đó, xét nhu cầu lâm sàng, việc bổ sung hai loại mẫu dò đề dò hai vi khuẩn Campylobacter jejuni và Yersinia enterocolitica O:8 gây bệnh đường ruột chủ yếu ở trẻ em, là cần thiết. Quy trình PCR-RDB được hoàn thiện trong nghiên cứu này vì vậy có khả năng phát hiện đồng thời 14 vi khuẩn gây bệnh đường ruột, một cách đặc hiệu, với độ nhạy đạt 10^2 bản sao/ml, đã được thử nghiệm trên 30 mẫu phân, ghi nhận kết quả hoàn toàn khớp với kít thương mại PowerCheckTM 20 Pathogen Multiplex Real-time PCR Kit (Korea).

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