Occurrence and Dispersal of Indicator Bacteria on Cucumbers Grown Horizontally or Vertically on Various Mulch Types

2016 ◽  
Vol 79 (10) ◽  
pp. 1663-1672 ◽  
Author(s):  
SHIRLEY A. MICALLEF ◽  
MARY THERESA CALLAHAN ◽  
SIVARANJANI PAGADALA

ABSTRACT No data exist on the impact of cultivation practices on food safety risks associated with cucumber. Cucumbers are typically grown horizontally over a mulch cover, with fruit touching the ground, but this vining plant grows well in vertical systems. To assess whether production system affects bacterial dispersal onto plants, field trials were conducted over 2 years. Cucumber cultivar ‘Marketmore 76’ was grown horizontally on plastic, straw, or bare ground or vertically on trellises installed on bare ground in soil previously amended with raw dairy manure. Fruit, flower, leaf, and soil samples were collected to quantify Escherichia coli, thermotolerant coliforms, and enterococci by direct plating. E. coli isolates were characterized by BOX-PCR to evaluate relatedness among strains. Although thermotolerant coliforms and enterococci were significantly less abundant on fruit in year 1 (P < 0.05), this result was not seen in year 2 when more rain was recorded. Instead, fruit from straw-mulched beds had higher levels of enterococci compared with fruit grown on bare ground (P < 0.05). Leaves on bare ground occasionally had more bacteria than did leaves on plastic mulch beds (P < 0.05). Production system did not impact flower-associated bacterial levels. E. coli isolates (n =127) were genotyped, generating 21 distinct fingerprints. Vertical production did not appear to be a barrier for E. coli dispersal to the crop, as suggested by numerous related isolates from soil and flowers on bare ground, straw-mulched, and trellised beds (subcluster B1). None of the isolates from soil and flowers in this subcluster were related to isolates recovered from fruit, showing that flower colonization does not necessarily lead to fruit colonization. One cluster of isolates contained those from flowers and fruits but not soil, indicating a source other than manure-amended soil. Straw may be a source of E. coli; a number of closely related E. coli isolates were retrieved from soil and fruits from straw-mulched beds. Our approach revealed E. coli dispersal patterns and could be used to assess bacterial transmission in other production systems.

Author(s):  
Greta G. Gramig ◽  
Samantha K. Hogstad ◽  
Patrick M. Carr

Abstract During 2015 and 2016, studies were conducted at Absaraka and Dickinson, North Dakota to evaluate the impacts of hemp (applied at 1156 m3 ha−1) and commercial paper mulch, as well as soil-applied biochar (applied at 11.25 m3 ha−1), on weed suppression and strawberry growth during the establishment year, and on weed suppression and strawberry yield during the production year, in a matted row production (MRP) system. During 2015, biochar influenced dry weed biomass only within the hemp mulch, with slightly more weed biomass associated with biochar application compared to zero biochar (3.1 vs 0.4 g m−2), suggesting that biochar may have increased weed germination and/or emergence from beneath hemp mulch. Biochar application also slightly increased soil pH, from 6.9 in non-amended soil to 7.0 in amended soil. Strawberry runner number during 2015 was greater in association with hemp or paper mulch compared to zero mulch (4.5 and 4.9 vs 2.4 runners plant −1, respectively). This result mirrored a similar differential in per berry mass across sites (7.6 and 7.4 vs 6.2 g berry −1 for hemp mulch, paper mulch and zero mulch, respectively). These results may be related to hemp and paper mulch reducing maximum soil temperatures during summer 2015. During the establishment year, both hemp and paper mulch suppressed weeds well compared to zero mulch, although at Absaraka hemp mulch provided slightly better weed suppression than paper mulch. During the production year, both mulches continued to suppress weeds compared to zero mulch at Dickinson. However, at Absaraka, only hemp mulch provided weed suppression compared to zero mulch, possibly because of faster paper degradation caused by greater numbers of large precipitation events and greater relative humidity at Absaraka compared to Dickinson. Weeds were removed from plots during 2015 to allow separation of weed suppression from other possible mulch impacts; therefore, yield data do not reveal striking differences among mulch treatments. Because previous research has demonstrated the impact of weed management during the establishment of strawberries in a matted row system, we concluded that hemp mulch may provide more durable weed suppression compared to paper mulch, which would increase strawberry yield protection in an MRP system. Material cost may be an issue for implementing hemp mulch, as hemp hurd cost was 25 times paper mulch at the application rates used in this study. However, hemp mulch could still be a beneficial option, especially for organic strawberry growers desiring a renewable and environmentally sound replacement for plastic mulch who are able to find affordable local sources of this material.


Author(s):  
Emre Bilgin Sarı ◽  
Sabri Erdem

Seru production system is a flexible, cost-effective, workforce competence-oriented manufacturing management system that provides the opportunity to respond quickly to customer demand. As in parallel to technology and physical improvements, customer demands are also effective for development of production systems. The impact of change in demand has been seen on changeover from job shop to mass production, flexible, and lean manufacturing systems. Seru production system is more appropriate for targeting work both cost-effectively like mass production and maximum diversification like job shop production. This chapter clarifies the Seru production system and explain its use and benefits in the clothing industry. In the application, a shirt production is illustrated according to the principles of mass production, lean production, and Seru production. Thus, different types of production systems have been benchmarked. There will be potential study areas for proving the efficiency of Seru soon.


2020 ◽  
Vol 12 ◽  
pp. 167-177 ◽  
Author(s):  
M Slater ◽  
E Fricke ◽  
M Weiss ◽  
A Rebelein ◽  
M Bögner ◽  
...  

Research into the effects of soundscapes on aquaculture species in key production systems is sparse, despite potential impacts of sound on animal welfare and commercial yields. In the following study, 2 high-value global aquaculture species, whiteleg shrimp Litopenaeus vannamei and Atlantic salmon Salmo salar, were exposed to aquaculture production system soundscapes. For shrimp, sound recordings of a commercial recirculating aquaculture system (RAS) were played back at a sound pressure level (SPL) of 128 dB re 1 µPa, and for salmon, recordings from a commercial sea pen production system were played back at an SPL of 127 dB re 1 µPa for an 8 wk period. Effects of exposure on growth, survival, and indications of metabolic stress were measured as parameters of interest for aquaculture production. Mean growth performance and survival rates did not differ significantly between sound and control treatments for either species. Blood and haemolymph parameters from both species indicated no measurable change in metabolic status or stress levels. Slight, but non-significant, increases in total haemocyte count and, in particular, hyaline cell count were recorded in shrimp exposed to sound. Slight, but non-significant reductions in overall weight gain were recorded in sound-exposed salmon. Overall, the results indicate that sound exposure in current production systems does not negatively affect the early grow-out stage of these key species, either due to rapid habituation or higher hearing thresholds of hatchery-produced individuals, and that no measurable stress response occurs in sound-exposed animals. In future studies, response of the studied species to acute sound exposure and the response of earlier, and potentially more sensitive, life-stages will need to be determined to ensure optimal welfare and production performance.


2019 ◽  
Vol 3 (2) ◽  
Author(s):  
L. G. Johnson ◽  
J. K. Grubbs ◽  
K. R. Underwood ◽  
M. J. Webb ◽  
A. D. Blair

ObjectivesBeef production systems utilize implants and β-agonists to improve beef cattle feed efficiency and promote muscle growth. Warner-Bratzler shear force values can be greater in strip loin steaks from cattle treated with implants or β-agonists. Calpain-1 degrades myofibrillar proteins post-mortem, thus altering calpain-1 activation or autolysis which can influence meat tenderness and proteolysis. The objective of this study was to determine the impact beef production system technologies on calpain-1 autolysis and troponin-T degradation as an indicator of tenderness formation and postmortem proteolysis.Materials and MethodsFrom a larger study, beef striploins (n = 16, n = 4/treatment) from cattle finished utilizing four different production systems were collected for analysis: 1) no antibiotics (NA; receiving no technology); 2) non-hormone treated cattle (NHTC; fed 300 mg monensin and 90 mg tylosin during the finishing phase); 3) implant (IMPL; same technologies as NHTC and administered a series of three implants including a low- potency calf implant [36 mg zeranol], a moderate-potency initial feedyard implant [80 mg trenbolone acetate and 16 mg estradiol], and a high potency finishing implant [200 mg of trenbolone acetate and 20 mg estradiol]; and 4) all previous technologies plus fed a β-agonist (IMBA; same technologies as IMPL and fed 200 mg ractopamine hydrochloride per steer per d). Striploins were vacuum packaged, aged for 7 d, and frozen. Western Blots were conducted for calpain-1 autolysis and troponin-T degradation (30 kDa). Abundance of calpain-1 bands and troponin-T degradation product was normalized by a reference on each gel. Treatments were evaluated in PROC MIXED of SAS 9.2 where least squares means and SEM were computed and separated using least significant differences (PDIFF) when tests for fixed effects were significant at P < 0.05 and trending P ≤ 0.10.ResultsCalpain-1 autolysis differed (P < 0.05) in the IMPL group compared to the NHTC group for both active, 78 kDa band, and the fully autolyzed, 76 kDa band. The IMPL group had a greater percentage (P = 0.0048) of active calpain-1 and a lower percentage (P = 0.0048) of fully autolyzed calpain-1 compared to the NHTC group. Also, a trend was detected when comparing both the active, 78 kDa band, and fully autolyzed, 76 kDa band, in the IMBA and IMPL group where the IMPL group had a greater percentage (P = 0.0727) of active calpain-1 and a lower percentage (P = 0.0727) of fully autolyzed calpain-1. Production system did not influence (P > 0.05) 30 kDa troponin-T product abundance.ConclusionThese data indicate level of technology may play a role in the activation and autolysis of calpain-1 from the 80 kDa inactive form to the 78 kDa active product and finally to the 76 kDa autolyzed product. Calpain-1 autolysis was not measured; however, these data suggest calpain-1 autolysis in the IMPL group may be limited compared with NHTC and IMBA groups. Consequently, calpain-1 may remain in the 78 kDa active form in the implanted cattle, actively degrading myofibrillar proteins. However, production system did not influence troponin-T 30 kDa degradation products. Further analysis of the rate of calpain-1 autolysis and troponin-T degradation at different days of postmortem aging could provide further evidence that different beef production technologies impact calpain-1 autolysis and postmortem proteolysis.


HortScience ◽  
2004 ◽  
Vol 39 (4) ◽  
pp. 893B-893
Author(s):  
Daniel Warnock* ◽  
Megan Fifarek ◽  
Heather Lash

The development of the Renaissance series of cut poinsettias, Euphorbia pulcherrima, presents unique opportunities and challenges to cut flower producers. This series has curled bracts, long stem length, excellent vase life and is highly marketable. Literature indicates that this crop is suited for pot or bed production, but does not compare methods. This study assessed the impact of production system on final stem quality. Uniform rooted cuttings of `Renaissance Red' obtained from a commercial supplier were transplanted into 30.5-cm pots or a 1.2-× 2.4-m bed containing a soilless media to obtain 2 plants per 0.9 m2. A total of 56 cuttings were used for each production system and grown using standard production techniques. Transplanting occurred on 11 Aug. 11 2003 with plants reaching market stage, two cyathia shedding pollen, about 17 weeks later. To minimize border effects, plants in the outside rows of each production system were discarded. Thus, stem length, stem diameter, bract diameter, floral development, and number of axillary shoots were determined for 30 interior plants in each system. The production systems differentially impacted stem length and number of axillary shoots. Mean stem length in the bed system (89.7 cm) was greater than that observed in the pot system (71.4 cm). Plants in the bed system had significantly fewer axillary branches per plant (0.5) than plants in the pots (2.0). Stem diameters were similar for both systems (11.5 mm and 10.9 mm for bench and pot, respectively) as were bract diameters (14.3 cm and 13.4 cm for pot and bench, respectively). Both systems produced marketable stems; however, stems produced in the bed system had longer stems, fewer axillary branches, and were more uniform than those in the pot system.


Author(s):  
Liaqat Ali ◽  
Shan Jin ◽  
Yong Bai

Abstract In past years, offshore oil and gas accidents have often occurred. Environmental hazards have the capability of turning into very difficult to manage in addition with the modern technology limits and lack of a fail-safe operation that can identify, control and terminate the accidents. However, the offshore crude oil also natural gas search and development is expanding to deep-water and moving promptly to the subsea production systems. (SPS). Though, the complicate subsea equipment material besides frequency offshore disasters stimulated the consideration onto the risk analysis of subsea systems. Detection of the impact of deep-water oil and gas reserves in the subsea production system. However, loss of SPSs can contribute to massive industrial failure, severe natural pollution, and indeed serious disasters. Therefore, the reliability analysis and safety of SPS have turned into a dominant consideration. This study addresses on the hazards and risk conditions which must be concentrated in the subsea machinery associated within surface equipments. Furthermore, the risks identification also the risk investigation onto subsea “Xmas tree” system is brought out. An over-all risk avert procedure of subsea “Xmas tree” system is represented, also the reliability evaluation method. Moreover, several recommendations on subsea production maintenance and detection are given in this research. This paper is reviewing the following section, subsea production system, hazards or risk identification, environmental issues, hydrate problems, corrosion problems, safety issues, risk assessment on subsea “Xmas tree”, reliability issues of a subsea system.


2017 ◽  
Vol 27 (1) ◽  
pp. 37-44 ◽  
Author(s):  
Christian A. Wyenandt ◽  
Wesley L. Kline ◽  
Daniel L. Ward ◽  
Nancy L. Brill

From 2006 to 2008, four different production systems and five bell pepper cultivars (Capsicum annuum) with either no resistance (Alliance and Camelot), tolerance (Revolution), or resistance (Paladin and Aristotle) to the crown rot phase of phytophthora blight (Phytophthora capsici) were evaluated for the development of skin separation or “silvering” in fruit at a research facility and four commercial vegetable farms in southern New Jersey. Cultivar, production system, and year, each had a significant effect on the total percentage of fruit with skin separation and marketable yield. The percentage of bell pepper fruit with skin separation was higher in both phytophthora-resistant cultivars compared with the phytophthora-susceptible cultivars across all four production systems. Marketable yield was highest when bell peppers were grown in double rows on raised beds with black plastic mulch and drip irrigation compared with bell peppers grown on single rows on raised beds with black plastic mulch and drip irrigation and bell peppers grown on single rows on raised, bare ground beds with buried drip irrigation. Marketable yields were lowest when bell peppers were grown in single rows on high, ridged beds with overhead irrigation. Results of this study suggest that the development of skin separation or “silvering” in fruit is more closely associated with genotype than type of production system.


2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Oscar Mencía-Ares ◽  
Héctor Argüello ◽  
Héctor Puente ◽  
Manuel Gómez-García ◽  
Edgar G. Manzanilla ◽  
...  

Abstract Background Antimicrobial resistance (AMR) is a global public health threat consequence of antimicrobial use (AMU) in human and animal medicine. In food-producing animals factors such as management, husbandry or biosecurity may impact AMU. Organic and extensive Iberian swine productions are based on a more sustainable and eco-friendly management system, providing an excellent opportunity to evaluate how sustained differences in AMU impact the AMR in indicator bacteria. Here, we evaluate the usefulness of commensal Escherichia coli and Enterococcus spp. isolates as AMR bioindicators when comparing 37 Spanish pig farms from both intensive and organic-extensive production systems, considering the effect of AMU and biosecurity measures, the last only on intensive farms. Results The production system was the main factor contributing to explain the AMR differences in E. coli and Enterococcus spp. In both bacteria, the pansusceptible phenotype was more common (p < 0.001) on organic-extensive farms when compared to intensive herds. The microbiological resistance in commensal E. coli was, for most of the antimicrobials evaluated, significantly higher (p < 0.05) on intensive farms. In enterococci, the lincosamides usage revealed the association between AMR and AMU, with an increase in the AMR for erythromycin (p < 0.01), quinupristin-dalfopristin (p < 0.01) and the multidrug-resistant (MDR) phenotype (p < 0.05). The biosecurity measures implemented on intensive farms influenced the AMR of these bioindicators, with a slightly lower resistance to sulfamethoxazole (p < 0.01) and the MDR phenotype (p < 0.05) in E. coli isolated from farms with better cleaning and disinfection protocols. On these intensive farms, we also observed that larger herds had a higher biosecurity when compared to smaller farms (p < 0.01), with no significant associations between AMU and the biosecurity scores. Conclusions Overall, this study evidences that the production system and, to a lesser extent, the biosecurity measures, contribute to the AMR development in commensal E. coli and Enterococcus spp., with antimicrobial usage as the main differential factor, and demonstrates the potential value of these bacteria as bioindicators on pig farms in AMR surveillance programs.


2002 ◽  
Vol 92 (12) ◽  
pp. 1367-1372 ◽  
Author(s):  
D. O. Chellemi

Nonchemical methods including host resistance, organic amendments, crop rotation, soil solarization, and cultural practices have been used to control soilborne pests in fresh market vegetable production systems. Their suitability as alternatives to methyl bromide will depend on the approach to pest management used by the grower. Traditionally, methyl bromide is used in production systems that rely on the single application of a broad-spectrum biocide to disinfest soils prior to planting. Non-chemical methods are not suitable for a single tactic approach to pest management because they do not provide the same broad spectrum of activity or consistency as fumigation with methyl bromide. Nonchemical methods are compatible with an integrated pest management (IPM) approach, where multiple tactics are used to maintain damage from pests below an economic threshold while minimizing the impact to beneficial organisms. However, adoption of IPM is hindered by the paucity of economically feasible sampling programs and thresholds for soilborne pests and by a reluctance of growers to commit additional resources to the collection and management of biological information. A novel approach to the management of soilborne pests is to design the crop production system to avoid pest outbreaks. Using this “proactive” approach, a tomato production system was developed using strip-tillage into existing bahia-grass pasture. By minimizing inputs and disruption to the pasture, growers were able to reap the rotational benefits of bahiagrass without cultivating the rotational crop. While minimizing the need for interventive procedures, a proactive approach is difficult to integrate into existing crop production systems and will require several years of testing and validation.


2012 ◽  
Vol 55 (1) ◽  
pp. 13-24 ◽  
Author(s):  
K. Brügemann ◽  
E. Gernand ◽  
U. König von Borstel ◽  
S. König

Abstract. The aim of this study was to assess the impact of heat stress in dairy cows on test-day records for production traits and somatic cell score (SCS) in the state of Lower Saxony, Germany. Three different production systems were defined: A production system characterized by intensive crop production (=indoor housing), a pasture based production system, and a maritime region. Heat stress was assessed by two temperature-humidity indices (THI) modelled as random regression coefficients in an analysis of variance: One (THIBo) was defined as an average of hourly THI, calculated from hourly recorded temperatures and humidities, the other (THIRa) was based on daily maximal temperature and daily minimal humidity. In all production systems, THIBo=60 and THIRa=70 were identified as general thresholds denoting a substantial decline in test-day milk yield. For daily fat and protein percentage, no universally valid thresholds were identified. In contrast for SCS, especially in the maritime region, heat stress as well as cold stress thresholds were found. Regression analysis was used to study the change in test day milk yield in response to THI of those THI ranges with an obvious decline in milk yield. Regression coefficients were −0.08 kg/THIBo and −0.16 kg/THIRa for the crop production system, −0.17 kg/THIBo and −0.23 kg/THIRa for the pasture based system, and −0.26 kg/THIBo and −0.47kg/THIRa for the maritime region. Based on statistical information criteria, identified thresholds for THIBo should be given preference over THIRa when applying genetic studies on heat stress in German Holstein cows.


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