Salmonella Contamination in Broiler Synovial Fluid: Are We Missing a Potential Reservoir?

ABSTRACT The objective of this study was to assess the presence and characteristics of Salmonella enterica found in the synovial fluid of broiler carcasses. The synovial fluid of three individual joints from 500 broiler carcasses was individually sampled from five broiler processing facilities located in the Southeast and West regions of the United States (1,500 total samples). The external surface of broiler carcass was decontaminated before sampling of the shoulder, coxofemoral, and tibiofemoral joints. Individual samples were enriched, composited, and subjected to rapid PCR-based detection of Salmonella. Individual samples from any positive composites were also enriched before determination of Salmonella presence in the same manner. Positive individual samples were subjected to secondary enrichment before plating onto selective agar for isolation of Salmonella. Salmonella isolates were serotyped before determination of antimicrobial susceptibility. Overall, 1.00% (5 of 500 broiler carcasses) of composite samples and 0.47% (7 of 1,500 samples) of individual samples were positive for Salmonella. Five of the seven isolates were susceptible to all drugs tested and determined to be Salmonella Enteritidis. The remaining two isolates, identified as Salmonella Typhimurium, were resistant to streptomycin. To our knowledge, no previous assessments of Salmonella in the synovial fluid of broilers has been reported; however, results of the present study suggested that the synovial fluid may be a reservoir for Salmonella in broilers. Although the prevalence of Salmonella is low, this information provides valuable insight into potential poultry contamination pathways and warrants further exploration.

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State-by-State Use of AMA Guides

Guides Newsletter ◽

10.1001/amaguidesnewsletters.2019.sepoct01 ◽

2019 ◽

Vol 24 (5) ◽

pp. 3-7, 16

Keyword(s):

Expert Testimony ◽

Narrow Range ◽

The United States ◽

Workers Compensation ◽

Federal Employees ◽

Permanent Disability ◽

Sixth Edition ◽

History Of ◽

Canadian Provinces

Abstract This article presents a history of the origins and development of the AMA Guides to the Evaluation of Permanent Impairment (AMA Guides), from the publication of an article titled “A Guide to the Evaluation of Permanent Impairment of the Extremities and Back” (1958) until a compendium of thirteen guides was published in book form in 1971. The most recent, sixth edition, appeared in 2008. Over time, the AMA Guides has been widely used by US states for workers’ compensation and also by the Federal Employees Compensation Act, the Longshore and Harbor Workers’ Compensation Act, as well as by Canadian provinces and other jurisdictions around the world. In the United States, almost twenty states have developed some form of their own impairment rating system, but some have a narrow range and scope and advise evaluators to consult the AMA Guides for a final determination of permanent disability. An evaluator's impairment evaluation report should clearly document the rater's review of prior medical and treatment records, clinical evaluation, analysis of the findings, and a discussion of how the final impairment rating was calculated. The resulting report is the rating physician's expert testimony to help adjudicate the claim. A table shows the edition of the AMA Guides used in each state and the enabling statute/code, with comments.

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Current perspectives in antithrombotic therapy

Hämostaseologie ◽

10.1055/s-0037-1619515 ◽

2001 ◽

Vol 21 (03) ◽

pp. 82-96 ◽

Cited By ~ 1

Author(s):

D. Hoppensteadt ◽

O. Iqbal ◽

R. L. Bick ◽

J. Fareed

Keyword(s):

Blood Coagulation ◽

Antithrombotic Therapy ◽

Thrombotic Event ◽

The United States ◽

Vascular Thrombosis ◽

Common Cause ◽

Coagulation Protein ◽

Coronary Events ◽

Platelet Defects

SummaryThrombotic disorders are the most common cause of death in the United States. About two million individuals die each year from an arterial or venous thrombosis or related disorders. About 80% to 90% of all cases of thrombosis can now be defined with respect to cause. Of these, over 50% occur in patients who harbor a congenital or acquired blood coagulation protein or platelet defect which caused the thrombotic event. It is obviously of major importance to define those individuals harboring such a defect as this allows: 1) appropriate antithrombotic therapy to decrease risks of recurrence; 2) determination of the length of time the patient must remain on therapy for secondary prevention; and 3) allow for testing of family members of those harboring a blood coagulation protein or platelet defect which is hereditary (about 50% of all coagulation and platelet defects mentioned above). Aside from mortality, significant additional morbidity occurs from both arterial or venous thrombotic events, including, but not limited to paralysis (non-fatal thrombotic stroke), cardiac disability (repeated coronary events), loss of vision (retinal vascular thrombosis), fetal waste syndrome (placental vascular thrombosis), stasis ulcers and other manifestations of post-phlebitic syndrome, etc.

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Impact of Rapid PCR Influenza Testing on the Rate of Inpatient Admissions During Influenza Season at a Tertiary-Care Center

Infection Control and Hospital Epidemiology ◽

10.1017/ice.2020.830 ◽

2020 ◽

Vol 41 (S1) ◽

pp. s263-s264

Author(s):

Jordan Polistico ◽

Avnish Sandhu ◽

Teena Chopra ◽

Erin Goldman ◽

Jennifer LeRose ◽

...

Keyword(s):

Influenza Season ◽

Care Center ◽

Tertiary Care ◽

The United States ◽

Turnaround Time ◽

Care Hospital ◽

Tertiary Care Center ◽

Pediatric Hospital ◽

Rapid Pcr ◽

The Impact

Background: Influenza causes a high burden of disease in the United States, with an estimate of 960,000 hospitalizations in the 2017–2018 flu season. Traditional flu diagnostic polymerase chain reaction (PCR) tests have a longer (24 hours or more) turnaround time that may lead to an increase in unnecessary inpatient admissions during peak influenza season. A new point-of-care rapid PCR assays, Xpert Flu, is an FDA-approved PCR test that has a significant decrease in turnaround time (2 hours). The present study sought to understand the impact of implementing a new Xpert Flu test on the rate of inpatient admissions. Methods: A retrospective study was conducted to compare rates of inpatient admissions in patients tested with traditional flu PCR during the 2017–2018 flu season and the rapid flu PCR during the 2018–2019 flu season in a tertiary-care center in greater Detroit area. The center has 1 pediatric hospital (hospital A) and 3 adult hospitals (hospital B, C, D). Patients with influenza-like illness who presented to all 4 hospitals during 2 consecutive influenza seasons were analyzed. Results: In total, 20,923 patients were tested with either the rapid flu PCR or the traditional flu PCR. Among these, 14,124 patients (67.2%) were discharged from the emergency department and 6,844 (32.7%) were admitted. There was a significant decrease in inpatient admissions in the traditional flu PCR group compared to the rapid flu PCR group across all hospitals (49.56% vs 26.6% respectively; P < .001). As expected, a significant proportion of influenza testing was performed in the pediatric hospital, 10,513 (50.2%). A greater reduction (30% decrease in the rapid flu PCR group compared to the traditional flu PCR group) was observed in inpatient admissions in the pediatric hospital (Table 1) Conclusions: Rapid molecular influenza testing can significantly decrease inpatient admissions in a busy tertiary-care hospital, which can indirectly lead to improved patient quality with easy bed availability and less time spent in a private room with droplet precautions. Last but not the least, this testing method can certainly lead to lower healthcare costs.Funding: NoneDisclosures: None

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Performance Testing of Coal Fired Power Plants

ASME 2007 Power Conference ◽

10.1115/power2007-22132 ◽

2007 ◽

Author(s):

Shane E. Powers ◽

William C. Wood

Keyword(s):

Power Plant ◽

Power Plants ◽

Performance Test ◽

Model Development ◽

Performance Testing ◽

The United States ◽

Plant Performance ◽

Cycle Performance ◽

Test Program

With the renewed interest in the construction of coal-fired power plants in the United States, there has also been an increased interest in the methodology used to calculate/determine the overall performance of a coal fired power plant. This methodology is detailed in the ASME PTC 46 (1996) Code, which provides an excellent framework for determining the power output and heat rate of coal fired power plants. Unfortunately, the power industry has been slow to adopt this methodology, in part because of the lack of some details in the Code regarding the planning needed to design a performance test program for the determination of coal fired power plant performance. This paper will expand on the ASME PTC 46 (1996) Code by discussing key concepts that need to be addressed when planning an overall plant performance test of a coal fired power plant. The most difficult aspect of calculating coal fired power plant performance is integrating the calculation of boiler performance with the calculation of turbine cycle performance and other balance of plant aspects. If proper planning of the performance test is not performed, the integration of boiler and turbine data will result in a test result that does not accurately reflect the true performance of the overall plant. This planning must start very early in the development of the test program, and be implemented in all stages of the test program design. This paper will address the necessary planning of the test program, including: • Determination of Actual Plant Performance. • Selection of a Test Goal. • Development of the Basic Correction Algorithm. • Designing a Plant Model. • Development of Correction Curves. • Operation of the Power Plant during the Test. All nomenclature in this paper utilizes the ASME PTC 46 definitions for the calculation and correction of plant performance.

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Determination of the Sensitivity of a RapidEscherichia coli O157:H7 Assay for Testing 375-Gram Composite Samples

Applied and Environmental Microbiology ◽

10.1128/aem.66.9.4149-4151.2000 ◽

2000 ◽

Vol 66 (9) ◽

pp. 4149-4151 ◽

Cited By ~ 16

Author(s):

Wan-Ling Tsai ◽

Cynthia E. Miller ◽

Edward R. Richter

Keyword(s):

Escherichia Coli ◽

Sample Size ◽

Ground Beef ◽

Immunomagnetic Separation ◽

Enzyme Linked Immunosorbent Assay ◽

Elisa System ◽

Composite Ground ◽

Single Size ◽

Composite Samples

ABSTRACT Both 25-g single-size ground beef samples and 375-g composite ground beef samples were tested by a method combining an immunomagnetic separation (IMS) technique with a sandwich enzyme-linked immunosorbent assay (ELISA) system (IMS-ELISA). The results demonstrated that IMS-ELISA could detect the target, Escherichia coliO157:H7, at the level of 10−1 CFU/g of sample in either the 25- or 375-g sample size.

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IMPROVEMENT OF THE CAMERAL CHECKS PROCESS IN RUSSIA BASED ON FOREIGN EXPERIENCE

Russian science: actual researches and developments,Part 1 ◽

10.46554/russian.science-2020.03-1-835/838 ◽

2020 ◽

Author(s):

Darya Sergeevna Kareva ◽

◽

Sevinj Mahmud kyzy Ismailova ◽

Elena Evgenievna Dozhdva ◽

◽

...

Keyword(s):

United States ◽

The United States ◽

Foreign Experience ◽

Correct Determination

The article substantiates the need to reform cameral tax control in Russia based on the experience of the United States. The necessity of introducing into the procedure of cameral control the mechanism of requesting all documents confirming the correct determination of tax obligations is determined. The aim of improving the process of desk audits of control is that the new procedure for conducting audits will reduce the likelihood of tax violations.

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Determination of the minimum suppressive concentration of photosensitizers of the second generation in relation to sanitaryindicative microorganisms

Veterinariya, Zootekhniya i Biotekhnologiya ◽

10.36871/vet.zoo.bio.202111005 ◽

2021 ◽

Vol 1 (11) ◽

pp. 42-46

Author(s):

Vadim Yu. Shlenskiy ◽

◽

Tatiana I. Pashnik ◽

Svetlana Yu. Pigina ◽

◽

...

Keyword(s):

Staphylococcus Aureus ◽

Antimicrobial Activity ◽

Salmonella Enteritidis ◽

Second Generation ◽

Research Work ◽

Protoporphyrin Ix ◽

Bactericidal Effect ◽

Disodium Salt ◽

Bacterial Cultures

In the studies described in the article, the presence of antimicrobial activity in photosensitizers in experiments on bacterial cultures was confirmed. In the experiments, photosensitizers of the second generation were used: «Photoditazine», the active substance of which is dimeglumin chloride E6, and «Dimegin», which includes a derivative of protoporphyrin IX-2,4-di(1-methoxyethyl)- deuteroporphyrin IX disodium salt. In the described research work, the minimum concentrations of the studied drugs were determined by the method of sequential tenfold dilutions to obtain a bactericidal effect on strains of sanitary-indicative microorganisms Enterococcus faecalis, Staphylococcus aureus, Salmonella enteritidis.

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