Assessment of Sanitation Practices for the Control of Listeria monocytogenes at Small and Very Small Ready-To-Eat Meat and Poultry Processors

2021 ◽  
Author(s):  
Sheena E Fate ◽  
Jeannine P Schweihofer ◽  
Tina Conklin
Keyword(s):  
Listeria Monocytogenes ◽  
Microbial Control ◽  
Control Measures ◽  
Personal Hygiene ◽  
Control Methods ◽  
Department Of Agriculture ◽  
Food Contact Surfaces ◽  
Content Domain ◽  
Single Temperature ◽  
Pathogen Control

Science-based guidance employed at eight small and very small state and federally inspected ready-to-eat (RTE) meat and poultry processors across Michigan was assessed. Data was collected to determine the current level of sanitary control methods used for reducing Listeria in the processing environment and compared interactions with the facility microbial results. A checklist was created to assess the current recommended sanitary control methods from the U.S. Department of Agriculture; U.S. Food and Drug Administration; and the Michigan Department of Agriculture and Rural Development. The checklist, comprised of 178 items divided into ten general content domains, was used to assess which of the recommended controls were being utilized in the facilities to prevent post lethality contamination of RTE products . Effectiveness of pre-operational and operational sanitation was assessed through sampling 12 non-food contact surfaces using an adenosine triphosphate (ATP) reader and amplified nucleic single temperature reaction test for Listeria spp., including Listeria monocytogenes at each facility. In total, 288 samples were taken collectively from the 8 facilities (96 ATP, 96 pre-operational  Listeria  spp.). Microbial outcomes did not differ ( P > 0.05 ) based on the overall number of recommended sanitary control methods utilized and type of facility inspection. There was a greater content domain compliance overall in operational sanitation ( P  = 0.0005), sanitation ( P  = 0.0030), facility ( P  = 0.0397) and personal hygiene ( P = 0.0033 ) than for segregation procedures regardless of the regulating body. Findings suggest that regardless of the regulating body, the quality of sanitary control measures utilized is more impactful for microbial control than simply the quantity implemented. Pathogen control may be obtained without implementing all of the sanitary control methods within the guidance documents.

scholarly journals Role of Cold Shock Proteins in Growth of Listeria monocytogenes under Cold and Osmotic Stress Conditions

2009 ◽  
Vol 75 (6) ◽  
pp. 1621-1627 ◽  
Author(s):  
Barbara Schmid ◽  
Jochen Klumpp ◽  
Eveline Raimann ◽  
Martin J. Loessner ◽  
Roger Stephan ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Osmotic Stress ◽  
Cold Shock ◽  
Microbial Control ◽  
Nacl Stress ◽  
Stress Conditions ◽  
Control Measures ◽  
Cold Shock Protein ◽  
Osmotic Stress Tolerance ◽  
Shock Proteins

ABSTRACT The gram-positive bacterium Listeria monocytogenes is a food-borne pathogen of both public health and food safety significance. It possesses three small, highly homologous protein members of the cold shock protein (Csp) family. We used gene expression analysis and a set of mutants with single, double, and triple deletions of the csp genes to evaluate the roles of CspA, CspB, and CspD in the cold and osmotic (NaCl) stress adaptation responses of L. monocytogenes. All three Csps are dispensable for growth at optimal temperature (37°C). These proteins are, however, required for efficient cold and osmotic stress tolerance of this bacterium. The hierarchies of their functional importance differ, depending on the environmental stress conditions: CspA>CspD>CspB in response to cold stress versus CspD>CspA/CspB in response to NaCl salt osmotic stress. The fact that Csps are promoting L. monocytogenes adaptation against both cold and NaCl stress has significant implications in view of practical food microbial control measures. The combined or sequential exposure of L. monocytogenes cells to these two stresses in food environments might inadvertently induce cross-protection responses.

scholarly journals Biological Control of a Phytosanitary Pest (Thaumatotibia leucotreta): A Case Study

2021 ◽  
Vol 18 (3) ◽  
pp. 1198
Author(s):  
Sean D. Moore
Keyword(s):  
Biological Control ◽  
Systems Approach ◽  
Microbial Control ◽  
Codling Moth ◽  
Environmental Safety ◽  
Control Measures ◽  
Sub Saharan Africa ◽  
The European Union ◽  
Thaumatotibia Leucotreta ◽  
Sub Saharan

Thaumatotibia leucotreta, known as the false codling moth, is a pest of citrus and other crops in sub-Saharan Africa. As it is endemic to this region and as South Africa exports most of its citrus around the world, T. leucotreta has phytosanitary status for most markets. This means that there is zero tolerance for any infestation with live larvae in the market. Consequently, control measures prior to exporting must be exemplary. Certain markets require a standalone postharvest disinfestation treatment for T. leucotreta. However, the European Union accepts a systems approach, consisting of three measures and numerous components within these measures. Although effective preharvest control measures are important under all circumstances, they are most critical where a standalone postharvest disinfestation treatment is not applied, such as within a systems approach. Conventional wisdom may lead a belief that effective chemical control tools are imperative to achieve this end. However, we demonstrate that it is possible to effectively control T. leucotreta to a level acceptable for a phytosanitary market, using only biological control tools. This includes parasitoids, predators, microbial control, semiochemicals, and sterile insects. Simultaneously, on-farm and environmental safety is improved and compliance with the increasing stringency of chemical residue requirements imposed by markets is achieved.

CONTROLLING SUFETULA SPP.: A COCONUT INSECT PEST ON PEAT SOILS

2007 ◽  
Vol 43 (3) ◽  
pp. 289-299 ◽  
Author(s):  
X. BONNEAU ◽  
M. HUSNI ◽  
L. BEAUDOIN-OLLIVIER ◽  
JOKO SUSILO
Keyword(s):  
Insect Pest ◽  
Bare Soil ◽  
Cultural Control ◽  
Control Measures ◽  
Control Methods ◽  
Peat Soils ◽  
Depressive Effect ◽  
Moss Cover ◽  
Adult Insect ◽  
The Impact

We demonstrated experimentally that Sufetula, a root-mining insect, has a depressive effect on coconut yields on peat soils. The impact of the pest resulted in a shortfall in earnings that warranted taking control measures. We considered control methods suitable for rehabilitating infested mature coconut plantings and for preserving young coconut plantings. Currently, cultural control is the only effective method. It involves eliminating all identified shelters for the adult insect, i.e. fern cover and heaps of coconut waste (dry fronds and husks). The aim is to achieve totally bare soil, with moss cover that does not attract the pest, or planted with an unattractive intercrop such as pineapple.

Establishment of incubation conditions to optimize the in vitro formation of mature Listeria monocytogenes biofilms on food-contact surfaces

Food Control ◽  
2018 ◽  
Vol 92 ◽  
pp. 240-248 ◽  
Author(s):  
C. Ripolles-Avila ◽  
A.S. Hascoët ◽  
A.E. Guerrero-Navarro ◽  
J.J. Rodríguez-Jerez
Keyword(s):  
Listeria Monocytogenes ◽  
Food Contact ◽  
Food Contact Surfaces ◽  
Contact Surfaces

Presence of Listeria monocytogenes in Ready-to-Eat (RTE) meat products sold at retail stores in Costa Rica and analysis of contributing factors

2021 ◽  
Author(s):  
Karol Calvo-Arrieta ◽  
Karol Matamoros-Montoya ◽  
María Laura Arias-Echandi ◽  
Alejandra Huete-Soto ◽  
Mauricio Alberto Redondo-Solano
Keyword(s):  
Listeria Monocytogenes ◽  
Costa Rica ◽  
Meat Products ◽  
Contributing Factors ◽  
Food Contact Surfaces ◽  
Handling Practices ◽  
The Moment ◽  
The Impact ◽  
Ammonium Compounds ◽  
Transfer Potential

Listeria monocytogenes is a pathogenic bacterium associated with RTE meat products sold at the retail level. The objective of this research was to determine the prevalence of Listeria monocytogenes in RTE meat products sold at retail in Costa Rica and to study the factors associated with the levels of contamination; analyzed factors include hygienic practices within stores (cutting techniques, microbial contamination of products) and the behavior of the isolates (persistence against antimicrobials and transfer potential). A total of 190 samples of RTE meat products were collected and analyzed for the presence of coliforms and Listeria species.  Isolates of L. monocytogenes were then evaluated in terms of resistance to disinfectants (quaternary ammonium compounds and chlorine) and their transfer potential from food contact surfaces (knife and cuttingboards). Overall Listeria spp. prevalence was 37,4% (71/190); L. innocua was present in 32,1% (61/190) of the products and L. monocytogenes was found in just 2,6% (5/190) of the samples. Most of the contaminated samples were cut with a knife at the moment of purchase (44,2%). When analyzing practices within the stores, it was observed that L. monocytogenes transfer from inoculated knife to “salchichón” was higher for samples cut right at the beginning of the experiment. Also, L. monocytogenes transfer from inoculated cuttingboards was independent of the number of slices but contamination from plastic was higher than wood. Regarding L. monocytogenes resistance to disinfectants, average reductions of 2,6 ± 1,1 log CFU/mL were detected after 6 minutes of exposure to 200 ppm of chlorine; however, chlorine resistance varied among the strains. Prevalence of L. monocytogenes in RTE meat products sold at retail could be associated with handling practices within the stores; further studies are necessary to estimate the impact of these practices on the overall risk for consumers.

The National Shipbuilding Research Program Welding Fume Study

2001 ◽  
Vol 17 (01) ◽  
pp. 35-39
Author(s):  
Daniel O. Chute ◽  
Bradley W. Christ
Keyword(s):  
Field Evaluation ◽  
Control Measures ◽  
Welding Fume ◽  
Control Methods ◽  
Exposure Control ◽  
Exposure Limits ◽  
Shipbuilding Industry ◽  
Worker Exposure ◽  
The Cost ◽  
The Impact

This paper presents the results of a study evaluating the impact of anticipated reductions in OSHA worker exposure limits for airborne contaminants, nickel (Ni), manganese (Mn), and hexavalent chromium (Cr6) on the shipbuilding industry. As part of the study field evaluation, air monitoring and data analysis were conducted to measure the effectiveness of a series of exposure control methods including fume extractor guns, ixed fume extraction systems, portable fume extraction systems, low fume welding wires, downdraft/backdraft tables, and fume iltration devices. Six shipyards participated in this study. The study concluded that the cost of compliance increases greatly with lower exposure limits. It was recommended that the shipbuilding industry continue to develop and evaluate feasible control measures in anticipation of revised standards.

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