scholarly journals Effects of sulfonamide antibiotics on digestion performance and microbial community during swine manure anaerobic digestion

2020 ◽  
Vol 26 (1) ◽  
Author(s):  
Shaona Wang ◽  
Kang Du ◽  
Rongfang Yuan ◽  
Huilun Chen ◽  
Fei Wang ◽  
...  

The effects of four types of sulfonamide antibiotics (SAs), including sulfaquinoxaline, sulfamethoxazole, sulfamethoxydiazine and sulfathiazole, on the digestion performance during anaerobic digestion process were studied using a lab-scale anaerobic sequencing batch reactor, and the changes of the community structure in the presence of SAs were investigated with the help of high throughput sequencing. The results indicated that when SAs were added, the hydrolytic acidification process was inhibited, and the accumulation of volatile fatty acids (VFAs) was induced, resulting in the suppression of methane production. However, the inhibition mechanism of different SAs was quite different. The inhibitory effect of high concentration of SAs on the hydrolysis of solid particulate matter into dissolved organic matter followed the order of sulfaquinoxaline > sulfamethoxydiazine > sulfathiazole > sulfamethoxazole. SAs have obvious inhibitory effects on acidification and methanation of dissolved organic matter, especially sulfathiazole. The richness and the community composition of the microorganism including bacteria and archaea in the digestion system were affected by SAs. Under the effect of SAs, the relative abundance of many microorganisms is negatively correlated with methane production, among which Methanobrevibacter, a kind of Archaea, had the greatest influence on methane production.

2000 ◽  
Vol 42 (10-11) ◽  
pp. 247-255 ◽  
Author(s):  
J. Paing ◽  
B. Picot ◽  
J. P. Sambuco ◽  
A. Rambaud

Sludge accumulation and the characteristics of anaerobic digestion in sludge had been investigated in a primary anaerobic lagoon. Methanogenic potential of sludge was evaluated by an anaerobic digestion test which measured the methane production rate. Sludge was sampled at several points in the lagoon to determine spatial variations and with a monthly frequency from the start-up of the lagoon to observe the development of anaerobic degradation. Maximum amounts of sludge accumulated near the inlet. The mean methane production of sludge was 2.9 ml gVS–1 d–1. Sludge near the outlet presented a greater methanogenic activity and a lesser concentration of volatile fatty acids than near the inlet. The different stages of anaerobic degradation were spatially separated, acidogenesis near the inlet and methanogenesis near the outlet. This staged distribution seemed to increase efficiency of anaerobic fermentation compared with septic tanks. Methane release at the surface of the lagoon was estimated to be very heterogeneous with a mean of 25 l m–2 d–1. The development of performance and sludge characteristics showed the rapid beginning of methanogenesis, three months after the start-up of the anaerobic lagoon. Considering the volume of accumulated sludge, it could however be expected that methanogenic activity would further increase.


Processes ◽  
2019 ◽  
Vol 7 (9) ◽  
pp. 590
Author(s):  
Tingting Zhong ◽  
Yali Liu ◽  
Xiaorong Kang

Batch tests were carried out to study the effect of simultaneous addition of ethylenediaminetetraacetic acid and Ni2+ (EDTA-Ni) on anaerobic digestion (AD) performances of kitchen wastes (KWs). The results indicated that the cumulative biogas yield and methane content were enhanced to 563.82 mL/gVS and 63.7% by adding EDTA-Ni, respectively, which were almost 1.15 and 1.07-fold of that in the R2 with Ni2+ addition alone. At the same time, an obvious decrease of propionic acid was observed after EDTA-Ni addition. The speciation analysis of Ni showed that the percentages of water-soluble and exchangeable Ni were increased to 38.8% and 36.3% due to EDTA-Ni addition, respectively. Also, the high-throughput sequencing analysis revealed that the EDTA-Ni promoted the growth and metabolism of Methanosarcina and Methanobacterium, which might be the major reason for propionic acid degradation and methane production.


2004 ◽  
Vol 50 (6) ◽  
pp. 109-114 ◽  
Author(s):  
D.V. Savant ◽  
D.R. Ranade

To operate anaerobic digesters successfully under acidic conditions, hydrogen utilizing methanogens which can grow efficiently at low pH and tolerate high volatile fatty acids (VFA) are desirable. An acid tolerant hydrogenotrophic methanogen viz. Methanobrevibacter acididurans isolated from slurry of an anaerobic digester running on alcohol distillery wastewater has been described earlier by this lab. This organism could grow optimally at pH 6.0. In the experiments reported herein, M. acididurans showed better methanogenesis under acidic conditions with high VFA, particularly acetate, than Methanobacterium bryantii, a common hydrogenotrophic inhabitant of anaerobic digesters. Addition of M. acididurans culture to digesting slurry of acidogenic as well as methanogenic digesters running on distillery wastewater showed increase in methane production and decrease in accumulation of volatile fatty acids. The results proved the feasibility of application of M. acididurans in anaerobic digesters.


2012 ◽  
Vol 531 ◽  
pp. 528-531 ◽  
Author(s):  
Na Wei

Anaerobic digestion is an economic and environmentally friendly technology for treating the biomass material-sewage sludge, but has some limitations, such as the low efficient biogass production. In this paper ultrasound was proposed as pre-treatment for effective sludge anaerobic digestion. Sludge anaerobic digestion experiments with ultrasonic pretreatment was investigated. It can be seen that this treatment effectively leaded to the increase of soluble chemical oxygen demand(SCOD) and volatile fatty acids(VFA)concentration. High concentration of VFA leaded to a increase in biogas production. Besides, the SV of sludge was reduced and the settling characteristics of sludge was improved after ultrasonic pretreatment. It can be concluded that sludge anaerobic digestion with ultrasonic pretreatment is an effective method for biomass material transformation.


2011 ◽  
Vol 64 (1) ◽  
pp. 70-76 ◽  
Author(s):  
D. Cysneiros ◽  
A. Thuillier ◽  
R. Villemont ◽  
A. Littlestone ◽  
T. Mahony ◽  
...  

Continuous Stirred Tank Reactors (CSTRs), operated in batch mode, were used to evaluate the feasibility of psychrophilic (low temperature) digestion of perennial rye grass in a long term experiment (150 days) for the first time. The reactors were operated in parallel at 3 different temperatures, 10, 15 and 37 °C. Hydrolysis, acidification and methanogenesis were assessed by VS degradation, by soluble chemical oxygen demand (SCOD) and volatile fatty acids (VFA) production, and by methane production, respectively. Hydrolysis was the rate-limiting step at all temperatures and the rates and extent of hydrolysis were considerably lower at 15 and 10 °C, than at 37 °C. The total VS degradation was 53%, 34% and 19% at 37, 15 and 10 °C, respectively. Acidification was not affected by temperature and VFA production and consumption was balanced in all cases, except at 10 °C. Methane yields were 0.215 m3 CH4 kg−1 VS−1 added, 0.160 m3 CH4 kg−1 VS−1 added and 0.125 m3 CH4 kg−1 VS−1 added at 37, 15 and 10 °C, respectively. Methanogenesis was not strongly affected at 15 °C but it became rate-limiting at 10 °C. Overall, the solid degradation and methane production performance under psychrophilic conditions was encouraging and greater than previously reported. Considering the non-acclimated, mesophilic nature of the inoculum, there are grounds to believe that low-temperature anaerobic digestion of grass could be feasible if coupled to efficient hydrolysis of the biomass.


2020 ◽  
pp. 0734242X2093961 ◽  
Author(s):  
Ning Du ◽  
Meng Li ◽  
Qian Zhang ◽  
Mihret Dananto Ulsido ◽  
Ruyi Xu ◽  
...  

The poorly controlled discharge of coffee husks in Ethiopia causes severe environmental pollution and is a waste of resources. The volatile solid and carbon content in coffee husks waste indicates that it is rich in organic matter and has huge potential to produce biogas. This study investigated the feasibility of coffee husks to produce biomass through anaerobic digestion, based on temperature, initial pH, inoculum/substrate (I/S) ratio and carbon/nitrogen (C/N) ratio. The study demonstrated that the maximum production of biogas and methane reached 3359.6 ml and 2127.30 ml, respectively, under the conditions of mesophilic temperature (35±1°C), an initial pH of 7, an I/S ratio of 0.75 and a C/N ratio of 30. Based on this result, the effects of trace elements (Fe2+, Ni2+, Co2+) on biogas production and methane content were also explored. Compared with the group with no addition of trace elements, the experiment adding trace elements had significant enhancement effects on the production of biogas and methane, in which Fe2+ played a leading role ( p<0.05). Fe2+ promoted the hydrolysis and acidification of coffee husks, resulting in the production of a series of intermediates such as volatile fatty acids and the other kinds of dissolved organic matter. Furthermore, the cooperation of Ni2+, Co2+ and Fe2+ enhanced the activity of the enzyme system in methanogens, promoting methane production. The results in this paper show that coffee husks have clear biogas potential through anaerobic digestion, and its effective utilization could fulfill the dual purpose of solid waste reclamation and local environmental protection in Ethiopia.


Processes ◽  
2019 ◽  
Vol 7 (7) ◽  
pp. 455 ◽  
Author(s):  
Rizwan Khan ◽  
Muhammad Ali Inam ◽  
Muhammad Akram ◽  
Ahmed Uddin ◽  
Sarfaraz Khan ◽  
...  

Engineered nanomaterials (ENMs), such as copper oxide nanoparticles (CuO NPs), are emerging as pollutants extensively used in many commercial and industrial applications, thus raising environmental concerns due to their release into water bodies. It is, therefore, essential to remove these pollutants from water bodies in order to minimize the potential threat to the aquatic environment and human health. The objective of this study was to investigate the removal of CuO NPs from waters by the coagulation process. This study also explored the efficiency of coagulation to remove hydrophobic/hydrophilic dissolved organic matter (DOM) and turbidity with varying polyaluminum chloride (PACl) doses. According to the results, a high concentration of DOM affects both the CuO NPs zeta potential and hydrodynamic diameter, thereby decreasing the agglomeration behavior. At effective coagulation zone (ECR), high removal of CuO NPs (>95%) was observed for all studied waters (hydrophobic and hydrophilic waters), above ECR excess charge induced by coagulant restabilized particles in solution. Furthermore, waters containing hydrophobic DOM and those with high UV254nm values needed more coagulant dose than hydrophilic waters to obtain similar CuO NP removals. The primary mechanism involved in CuO NPs removal might be charge neutralization. These findings suggest that PACl is an effective coagulant in the removal of CuO NPs; however, water characteristics are an influencing factor on the removal performance of ENMs during the coagulation process.


2011 ◽  
Vol 356-360 ◽  
pp. 2510-2514 ◽  
Author(s):  
Ming Fen Niu ◽  
Sai Yue Wang ◽  
Wen Di Xu ◽  
An Dong Ge ◽  
Hao Wang

In order to improve the rate of degradation of cellulose in corn straw, the study has an important significance that compost corn straw with inoculating high-efficient microbe agents. The experiment inoculated a cellulose-degrading strain F2 which was screened from compost into compost pretreatment, the VS of corn straw reduced from 93.14% to 71.69% after 15 days, the content of cellulose reduced from 34.12g·kg-1 to 25.66g·kg-1, the rate of degradation was 24.79% which was 10.60% higher than those without the strain. An anaerobic fermentation experiment was carried out with the two groups of composted corn straw and mixed pig feces with a certain ratio, and investigations of biogas production, pH, content of volatile fatty acids(VFA) and rate of methane production were conducted. The results were that the corn straw composted with the cellulose-degrading strain peaked 4 days earlier, the maximal daily biogas production was 1470mL, the cumulative biogas production reached 23641mL which was 16.87% higher and operated stably earlier. The study showed that the cellulose-degrading strain had a strong capacity to degrade cellulose in corn straw, and then improved the performance of anaerobic digestion.


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