scholarly journals Female and Male Wistar Rats (Rattus norvegicus) Discriminate Diets According to Energetic Quantity

2020 ◽  
Vol 33 ◽  
Author(s):  
Vinícius Warisaia ◽  
Vítor Pansarim ◽  
Davi C. Aragon ◽  
Fernando S. Zucoloto ◽  
Andréia Schmidt
Keyword(s):  
Rattus Norvegicus ◽  
Food Choice ◽  
Wistar Rats ◽  
Home Cage ◽  
Response Cost ◽  
Significant Preference ◽  
Male Wistar Rats ◽  
Flavor Test

The food choice of animals is influenced by several factors including the quantity and nutrients available. It is not known, however, whether faced with alternatives that present the same amount of food, with similar flavor and obtained with the same response cost, rats would discriminate between diets with different energetic quantities. The aim was to verify whether female and male Wistar rats (Rattus norvegicus) discriminate between three types of food that differ in their energetic content (whether or not they prefer one) and whether the flavor could affect the choice between two diets with equal energetic quantities. Twelve Wistar rats (six of each sex) underwent tests of choice between pairs of diets of different energetic values. After the tests, the animals had at their disposal, in the home cage, two diets with the same energetic content, which differed in flavor (one contained sucrose) - Flavor test. The consumption of each diet was measured for five consecutive days. All the subjects demonstrated a preference for the more energetic alternative, regardless of the combination of diets presented. In the Flavor test the animals did not show significant preference for any diet, i.e., the consumption of both the S and N diets were statistically equal for all subjects. It was concluded that the animals, regardless of sex, discriminated between the diets with different energetic values and that the flavor did not seem to be a determinant variable in the food choice.

scholarly journals Effect of Caffeic Acid Phenethyl Ester Provision on Fibroblast Growth Factor-2, Matrix Metalloproteinase-9 Expression, Osteoclast and Osteoblast Numbers during Experimental Tooth Movement in Wistar Rats (Rattus norvegicus)

2021 ◽  
Author(s):  
Ida Bagus Narmada ◽  
Paristyawati Dwi Putri ◽  
Lucky Lucynda ◽  
Ari Triwardhani ◽  
I Gusti Aju Wahju Ardani ◽  
...  
Keyword(s):  
Growth Factor ◽  
Matrix Metalloproteinase ◽  
Rattus Norvegicus ◽  
Wistar Rats ◽  
Tooth Movement ◽  
Caffeic Acid Phenethyl Ester ◽  
Fibroblast Growth Factor 2 ◽  
Fibroblast Growth ◽  
Male Wistar Rats ◽  
Metalloproteinase 9

Abstract Objectives To investigate the effect of caffeic acid phenethyl ester (CAPE) provision on matrix metalloproteinase-9 (MMP-9), fibroblast growth factor-2 (FGF-2) expression, osteoclast and osteoblast numbers during experimental orthodontic tooth movement (OTM) in male Wistar rats (Rattus norvegicus). Materials and Methods Forty-eight healthy male Wistar rats (R. norvegicus), 16 to 20 weeks old with 200 to 250 g body weight (bw) were divided into several groups as follows: K1: OTM for 3 days; K2: OTM for 7 days; K3: OTM for 14 days; KP1: OTM and CAPE for 3 days; KP2: OTM and CAPE for 7 days; and KP3: OTM and CAPE for 14 days. A nickel titanium closed coil spring 8.0 mm long with 10 g/mm2 was installed between the upper left first molar and upper central incisor to move molar mesially. CAPE provision with a dose of 20 mg/kg bw of animal studies was done per orally. Immunohistochemistry was done to examine MMP-9 expression and osteoclast number in compression side as well as FGF-2 expression and osteoblast number in tensile side of the OTM. Statistical Analysis One-way analysis of variance test and Tukey’s honest significant difference test were performed to determine the difference between the groups (p < 0.05). Results MMP-9 expression and osteoclast numbers in the compression side were significantly different between the groups. Similarly, FGF-2 expression and osteoclast numbers in the tensile side were significantly different between the groups. Conclusions CAPE provision during OTM increases the number of osteoblasts and the FGF-2 expression significantly in the tensile side. Osteoclast numbers and MMP-9 expression significantly decrease in the compression side.

scholarly journals Ekstrak Etanol Daun Jati Belanda (Guazuma ulmifolia Lamk) Memperbaiki Profil Lipid Tikus (Rattus norvegicus) Wistar Jantan Dislipidemia

2020 ◽  
Vol 1 (1) ◽  
pp. 25-30
Author(s):  
Melita Hidajat ◽  
I Gusti Made Aman ◽  
Hendro Sukoco ◽  
Ferbian Milas Siswanto
Keyword(s):  
Treatment Group ◽  
Lipid Profile ◽  
Total Cholesterol ◽  
Rattus Norvegicus ◽  
Wistar Rats ◽  
Control Group ◽  
Wistar Strain ◽  
Before And After ◽  
Male Wistar Rats ◽  
Guazuma Ulmifolia

The purpose of this study was to prove that the administration of Jati (Guazuma ulmifolia Lamk) leaves extract improves the lipid profile of dyslipidemic male Wistar rats. Subjects were 20 rats (Rattus norvegicus), male, Wistar strain, dyslipidemia (total cholesterol ≥ 200 mg dl-1), aged 2 months old, weighing 180-200 grams. The control group (10 rats) were given a placebo of 3 ml aquadest (P0) and the treatment group was given extracts of the Jati (Guazuma ulmifolia Lamk) leaves extract of 25 mg kg-1 BW (P1). Before and after treatment for 14 days, total cholesterol, triglyceride, LDL, and HDL levels were examined. The results showed that in the P0 group there were no changes in total cholesterol, triglyceride, LDL, and HDL levels (p>0.05), whereas the P1 group experienced a decrease in total cholesterol, triglyceride and LDL levels (p<0.05) and an increase in HDL levels (p<0.05). The results of this study indicated that the Jati leaves extract was effective to improve the lipid profile of dyslipidemic rats. It was necessary to compare the effectiveness of Jati leaves extract with synthetic dyslipidemia drugs used in the community such as statin.

Chronic administration of gabapentin and a gabapentin-carbamazepine combination reversibly suppress testicular function in male Wistar rats ( Rattus norvegicus )

2017 ◽  
Vol 24 (2) ◽  
pp. 63-69 ◽  
Author(s):  
Opeyemi Samson Osuntokun ◽  
Gbola Olayiwola ◽  
Ayowole Oladele ◽  
Idowu Ola ◽  
Oludele AyokaAbiodun
Keyword(s):  
Rattus Norvegicus ◽  
Wistar Rats ◽  
Chronic Administration ◽  
Testicular Function ◽  
Male Wistar Rats

scholarly journals The Effect of Soy Milk on Mounting Latency, Mounting Frequency, and Reproductive Development in Male Wistar Rats (Rattus Norvegicus)

2021 ◽  
Vol 9 (B) ◽  
pp. 670-678
Author(s):  
Nurdiana Nurdiana ◽  
Pradnyawati Chania ◽  
Rifzi Nurvitasari ◽  
Azmiatun Nisa ◽  
Styan Wahyu Diana ◽  
...  
Keyword(s):  
Rattus Norvegicus ◽  
Wistar Rats ◽  
Reproductive Development ◽  
Normal Diet ◽  
Male Rats ◽  
Male Rat ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Soy Milk ◽  
Male Wistar Rats

AIM: This research aims to examine the effects of soy milk on mounting latency (ML), mounting frequency (MF), estrogen levels, androgen-binding protein (ABP) expression, and spermatogenesis in male rats (Rattus norvegicus). METHODS: Twenty-four male wistar rats (Rattus norvegicus) aged 4 weeks were divided into four groups. Control group (given a normal diet), P1; P2; P3 (given the normal diet and soy milk powder at doses of 7.1; 14.2; 21.3 g/KgBW/day, respectively) for 6 weeks. Observation of ML and MF were performed at 9 weeks 5 days of age, and rat surgery was performed at 10 weeks of age. Analysis of estrogen hormone levels was conducted by enzyme-linked immunosorbent assay (ELISA), ABP staining was using immunohistochemistry method, testicular spermatogenesis was observed using histopathological methods, and observation of spermatozoa was performed under the microscope.  RESULTS: The results showed no significant reduction of ML and MF, estrogen levels, and ABP expression (p ≤ 0.256; 0.865; 0.959, respectively) in male rat, but there was a significant decrease in the number, morphology, motility of spermatozoa, and testicular histophatology, (p ≤ 0.000, 0.003, 0.008, 0.000, respectively). CONCLUSION: The administrassion of soy milk in various doses (7.1;14.2;21.3 g/KgBW/day) in male Wistar rats (Rattus norvegicus) had showed significantly difference on histopathological evaluation using Johnson’s scoring system, sperm quantity and quality, while on mounting latency and frequency, estrogen levels, and ABP expressions did not show significantly difference between groups. That describe of isoflavone in soy milk can affect several aspects related to male endocrine and reproductive development.

scholarly journals PENGARUH JAMU DENGAN Tribulus terrestris TERHADAP KUALITAS SPERMA TIKUS WISTAR JANTAN (Rattus norvegicus)

2015 ◽  
Vol 3 (2) ◽  
Author(s):  
Delano Pelealu ◽  
Lydia Tendean ◽  
Benny Wantouw
Keyword(s):  
Experimental Study ◽  
Rattus Norvegicus ◽  
Wistar Rats ◽  
Sperm Morphology ◽  
Sperm Quality ◽  
Control Group ◽  
Tribulus Terrestris ◽  
Normal Morphology ◽  
Normal Sperm ◽  
Male Wistar Rats

Abstract: It is known that Tribulus terrestris can improve sperm quality. One of the Indonesian traditional medicine, jamu, contains this Tribulus terrestris. This study aimed to investigate the effect of jamu containing Tribulus terrestris on sperm quality which consisted of concentration, motility, and morphology of spermatozoa of male wistar rats (Rattus norvegicus). This was an experimental study. Nine male wistar rats were randomly divided into 3 groups of 3 rats each: Group P0 (control), group P1 (given 5 mg of Tribulus terrestris), and group P2 (given 10 mg of Tribulus terrestris). This study was conducted for 52 days. The results showed that the spermatozoa concentration of P1 was 49x106 sperm/ml suspension and of P2 53x106 sperm/ml suspension; both were lower than of P0 (59x106 sperm/ml suspension). The sperm motility category (A+B) of P1 was 33% and of P2 (37%); both were lower than of P0 (45%). The motility category A was not found in P1 and P2. The percentage of spermatozoa with normal morphology of P1 was 45% and the abnormal ones was 55%, meanwhile in P2 there was 49% of normal sperm morphology and 51% of abnormal morphology. Conclusion: Jamu containing Tribulus terrestris had no effect in improvement of the sperm quality.Keywords: jamu, Tribulus terrestris, sperm qualityAbstrak: Tribulus terrestris dikenal sebagai bahan yang dapat memperbaiki kualitas sperma. Salah satu jenis jamu yang diproduksi di Indonesia mengandung Tribulus terrestris Penelitian ini bertujuan untuk mengetahui pengaruh jamu dengan Tribulus terrestris terhadap konsentrasi, motilitas, dan morfologi spermatozoa tikus wistar jantan (Rattus norvegicus). Penelitian ini menggunakan metode eksperimental. Sampel 9 ekor tikus wistar jantan (Rattus norvegicus) dibagi menjadi 3 kelompok yakni, kelompok P0 (kontrol); kelompok P1 (pemberian Tribulus terrestris 5 mg); dan kelompok P2 (pemberian Tribulus terrestris 10 mg). Penelitian dilakukan selama 52 hari. Hasil penelitian menunjukkan bahwa konsentrasi spermatozoa P1 49x106 sperma/ml suspensi dan P2 53x106 sperma/ml suspensi lebih rendah dibandingkan P0 59x106 sperma/ml suspensi. Motilitas spermatozoa kategori A+B pada P1 (33%) dan P2 (37%) lebih rendah dibandingkan pada P0 (45%). Tidak ditemukan motilitas kategori A pada P1 dan P2. Morfologi spermatozoa normal pada P1 sebesar 45% dan abnormal 55%. P2 memiliki morfologi spermatozoa normal sebesar 49% dan abnormal 51%. Simpulan: Pemberian jamu dengan Tribulus terrestris tidak meningkatkan kualitas sperma yang mencakup konsentrasi, motilitas, dan morfologi spermatozoa.Kata kunci: jamu, Tribulus terrestris, kualitas sperma

scholarly journals Ulva lactuca Linnaeus Potentially Promotes Reproductive Indices and Depressive-like Behavior of Hypertriglyceridemia Male Wistar Rats (Rattus norvegicus Berkenhout, 1769)

2020 ◽  
Vol 5 (3) ◽  
pp. 228
Author(s):  
Anggoro Chandra Yulistiyanto ◽  
Mayumi Hersasanti ◽  
Rahadian Yudo Hartantyo ◽  
Laksmindra Fitria ◽  
Abdul Razaq Chasani ◽  
...  
Keyword(s):  
Body Weight ◽  
Rattus Norvegicus ◽  
Wistar Rats ◽  
Gonadosomatic Index ◽  
Ulva Lactuca ◽  
The Body ◽  
Physiological Functions ◽  
Testosterone Concentration ◽  
Natural Treatment ◽  
Male Wistar Rats

Excessive consumption of fatty foods can lead to hyperlipidemia, which is often coupled with hypertriglyceridemia (HTG), a condition where blood plasma triglyceride (TG) levels elevated beyond normal levels. This condition may disturb physiological functions of the body, such as reproductive functions, and other physiological imbalances leading to chronic stress and depression. Ulva lactuca is a potential natural treatment for HTG, as it contains various nutrients to aid physiological functions. This seaweed also has high levels of Cd, which can increase depression. Therefore, research on the potential benefits of U. lactuca should be followed by an investigation of its health risks. This research aimed to examine the effects of HTG and treatment with U. lactuca on reproduction and depressive-like behavior of male Wistar rats (Rattus norvegicus Berkenhout, 1769). The data collected in this research include body weight, serum TG concentration, gonadosomatic index (GSI), serum testosterone concentration using competitive ELISA, and depressive-like behaviors assessed using the Forced Swim Test (FST) and Open Field Test (OFT). Data were analyzed using One-Way ANOVA followed by DMRT, independent- and paired-samples t-test, and Kruskal-Wallis H test with a significance value of α=0.05. Body weight, serum TG and testosterone concentration, GSI, and depressive-like behaviors were increased by the HTG condition. Ulva lactuca at the1500 mg/kg BW/day did not significantly affect body weight, testosterone concentration, and depressive-like behaviors of HTG rats. Meanwhile, this treatment significantly increased the GSI and depressive-like behaviors of healthy rats. These results suggest that Ulva lactuca treatment not only enhances gonad growth and development but also increases depressive-like behaviors.

scholarly journals Pemberian ekstrak daun sirih (Piper betle) secara topikal meningkatkan ketebalan epidermis, jumlah fibroblas, dan jumlah kolagen dalam proses penyembuhan luka pada tikus jantan galur Wistar (Rattus norvegicus)

2017 ◽  
Vol 5 (1) ◽  
Author(s):  
Eva F. Palumpun ◽  
Anak A.G.P. Wiraguna ◽  
Wimpie Pangkahila
Keyword(s):  
Rattus Norvegicus ◽  
Wistar Rats ◽  
Healing Process ◽  
Wound Healing Process ◽  
Piper Betle ◽  
Epidermal Thickness ◽  
Betel Leaf ◽  
Oral Amoxicillin ◽  
Male Wistar Rats ◽  
Sirius Red

Abstract: This study was aimed to prove that topical betel (Piper betle) leaf extract adinistration could increase epidermal thickness, fibroblasts, and collagen amount in wound healing process of male Wistar rats (Rattus norvegicus). Subjects were 36 Wistar rats (Rattus norvegicus) with inclusion criteria, as follows: healthy, aged 3-4 months, weighing 200-250 g, divided into two groups with 18 rats each. The first group, the control group (P0), was treated with oral amoxicillin 3x10mg/day for 3 days and one drop (50 μl) of 10% povidine iodine topically 2x/day for 14 days, and the second group, the treatment group (P1), treated with oral amoxicillin 3x10 mg/day for 3 days and one drop (50 μl) of 10% betel leaf (Piper betle) extracttopically 2x/day for 14 days. Samples of skin tissue were processed for histological slides by using hematoxylin-eosin staining to check the epidermal thickness and fibroblast, meanwhile Picro sirius red staining to check the collagen amount. Microscopic examinations showed that the average epidermal thickness in P0 group was 24.72±14.91 μm, whereas in the P1 group was 56.75±23.04 μm (P <0.01). The number of fibroblasts in P0 group was 75,45±32,52 cells/visual field meanwhile of P1 group was 95,67±22,51 cells/visual field (P < 0.05). The average of collagen amount in P0 group was 65.27±7.13% while in P1 group was 83.09±2.59% (P <0.01). Conclusion: Topical administration of 10% betel (Piper betle) leaf extract could increase epidermal thickness, fibroblasts, and collagen in wound healing process of male Wistar rats (Rattus norvegicus).Keywords: betel leaf, epidermis, fibroblast, collagen, wound Abstrak: Tujuan penelitian ini ialah untuk membuktikan bahwa pemberian ekstrak daun sirih (Piper betle) meningkatkan ketebalan epidermis, jumlah fibroblas, dan jumlah kolagen dalam proses penyembuhan luka pada tikus jantan galur Wistar (Rattus norvegicus). Subjek penelitian ialah 36 ekor tikus jantan galur Wistar (Rattus norvegicus) dewasa dan sehat, berumur 3-4 bulan, dengan berat badan 200-250 gr, yang terbagi menjadi 2 (dua) kelompok masing-masing berjumlah 18 ekor tikus. Kelompok pertama ialah kelompok kontrol (P0) diberikan amoksisilin oral 3 x 10mg/hari selama 3 hari serta povidine iodine 10% topikal 1 tetes (50 μl) 2x/hari selama 14 hari (P0). Kelompok kedua ialah kelompok perlakuan (P1) diberi amoksisilin oral 3 x 10 mg/hari selama 3 hari serta ekstrak daun sirih (Piper betle) konsentasi 10% secara topikal 1 tetes (50 μl), 2x/hari selama 14 hari. Jaringan kulit diambil dan dibuat preparat dengan pewarnaan hematoksilin-eosin untuk pemeriksaan ketebalan epidermis dan jumlah fibroblas, serta pewarnaan Picro sirius red untuk pemeriksaan jumlah kolagen. Hasil pemeriksaan mikroskopik menunjukkan rerata tebal epidermis pada kelompok P0 24,72±14,91 μm dan pada kelompok P1 56,75±23,04 μm (P <0,01). Rerata jumlah fibroblas pada kelompok P0 75,45±32,52 sel/lapang pandang dan pada kelompok P1 95,67±22,51 sel/lapang pandang (P <0,05). Rerata jumlah kolagen pada kelompok P0 65,27±7,13% dan pada kelompok P1, 83,09±2,59% (P <0,01). Simpulan: Pemberian ekstrak daun sirih (Piper betle) konsentrasi 10% secara topikal dapat meningkatkan ketebalan epidermis, jumlah fibroblas, dan jumlah kolagen pada luka tikus jantan galur Wistar (Rattus norvegicus). Kata kunci: daun sirih, epdermis, fibroblas, kolagen, luka

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