DETERMINATION OF FUNGI RESISTANCE OF SEVERAL SAMPLES OF NATURAL LEATHER IN CONDITIONS OF ARMENIA

2021 ◽  
Vol 55 (2 (255)) ◽  
pp. 172-180
Author(s):  
Iren V. Shahazizyan ◽  
Inessa M. Eloyan ◽  
Ruzanna E. Matevosyan ◽  
Siranoush G. Nanagyulyan

In this work we studied the effect of microscopic soil fungi on some lather samples. The species composition of micromycetes inhabiting the samples under study was revealed, and an assessment of the fungal resistance of the materials under study was given. In the process of work, species of microscopic soil fungi were isolated and identified from the soil. In order to determine the degree of resistance to fungi for the infection of samples, a water-spore suspension was obtained. The leather materials were partially destroyed by microscopic fungi, and mold resistance ranged from 2 to 3 on a 5-point scale.

Author(s):  
G. P. Kononenko ◽  
◽  
E. A. Piryazeva ◽  
E. V. Zotova ◽  
Sh. I. Razokov ◽  
...  

The article presents the results of complex study of cotton-seed cake from the Republic of Tajikistan, including the determination of content of mycotoxins, the analysis of exposure to microscopic fungi and the assessment of the potential of their toxin formation. The ability to produce mycotoxins was established for 11 species of micromycetes belonging to the genus Aspergillus, Penicillium, Fusarium, Scop-ulariopsis, and Trichothecium. Alternariol, zearalenone, fu-monisins, aflatoxin B1, sterigmatocystin, ochratoxin A, citrinin and emodin were found in the composition of the cotton-seed cake. Prospects for further research and the practical significance of the results are discussed.


2021 ◽  
Vol 27 ◽  
Author(s):  
Awad Shala ◽  
Shweta Singh ◽  
Saif Hameed ◽  
.M.P. Khurana

: Candida albicans is one of the main agents responsible for opportunistic pathogenic infections. The progressive emergence of fungal resistance to conventional antibiotics and its side effects as well as treatment costs are considered as major limitations for antifungal drugs. It has drawn scientists' attention to search for potential substitution and therapeutic reliable alternatives for the antifungal compounds from sources like medicinal plants, which contain numerous bioactive compounds such as essential oils. Essential oils (EO) apart from having lower toxicity and better biodegradability are eco-friendly in nature as compared with conventional antibiotics. Furthermore, extracted essential oils have been reported to possess potent antimicrobial, anti-inflammatory and antioxidant properties that nominate them as natural promising candidates to combat numerous fungal ailments. Thus, determination of antifungal efficacy of essential oil-bearing plants on Candida spp. will provide miscellaneous knowledge for future clinical studies that are required for development of new formulations as alternative therapeutic agents to control the growth of Candida species. Therefore, this review summarizes the gist of major essential oils that have been investigated for their anti- Candida potential with some recommendations for further study.


2020 ◽  
pp. 12-16
Author(s):  
Yu. Klechkovskyi ◽  
K. Shmatkovska

Goal. To determine the species composition, dominant species, and territorial distribution of predatory and herbivorous ticks (phytophages) in the vineyards of southern Ukraine. Methods. Field — route surveys of vineyards to study the fauna, trophic links and colonization of vineyards with ticks. Laboratory — determination of the species composition of representatives of the acarofauna of vineyards. Results. During the study period, 17 species of ticks belonging to different trophic groups were identified, among which ticks dominate — phytophages of the Tetranychidae and Eriophyidae families. The most common among them were mites — phytophages, namely spider mites (Shhizotetranychus Oud., Tetranychus turkestanicus Ug.et Nich). Pests during the growing season go through four phases: prodromal, eruptive, gradation maximum and crisis. The graduation maximum phase, which is characterized by their greatest number, was noted during the period of intensive growth of berries and falls at the end of June — beginning of August (BBCH 71—79). The dynamics of the number of ticks by varieties is shown. The varieties most susceptible to spider mites were identified, the period of technical ripeness of which is medium, late or very late. Conclusions. Identified 17 species of ticks belonging to different trophic groups: phytophages, acariphages and mycophages. Based on observations of changes in the number of pests on vineyards during the growing season, four phases are distinguished. The graduation maximum phase occurs at the end of June — beginning of August (VVSN 71—79). It was established that all the studied grape varieties were populated by mites to varying degrees, the most susceptible were varieties of medium and late ripening.


Author(s):  
Miroslava Kačániová ◽  
Janka Sudzinová ◽  
Miriam Kádasi-Horáková ◽  
Magdaléna Valšíková ◽  
Stanislav Kráčmar

The plant-microbial interactive relations with respect to determination of the mycoflora of theCastanea sativaMill. nuts, crust, leaves and pollen and their effect on the host organism in four Slovak regions were studied. In the experiments were isolated 7 genera and 10 species of microscopic fungi from the nut, crust and leaves. It was found, that isolates from theCastanea sativaMill. pollen were represented by 8 genera and 11 species of microscopic fungi.Alternaria,Cladosporium,MucorandRhizopusappeared to be the most frequently occurring genera of nuts, leaves and crust.Acremonium,Alternaria,Cladosporium,Fusarium,PenicilliumandTrichodermaare the most frequently fungi of pollen. On the base of further taxonomic determination from the generaAspergilluswere isolated and identified representatives of speciesA. flavus,A. fumigatus,A. ochraceus,A. terreusandA. versicolor. From the generaFusariumwas isolatedF. oxysporumand fromPenicilliumgenera were isolatedP. crustosumandP. glabrum. It is necessary to underline that the isolated generaAspergillus,FusariumandPenicilliumare considered as the most important producers of mycotoxins.


2015 ◽  
Vol 41 (2) ◽  
pp. 41-46 ◽  
Author(s):  
Ewa Miaśkiewicz-Pęska ◽  
Mirosław Szyłak-Szydłowski

Abstract Microbiological and chemical analysis of air was carried out on the area of landfill of wastes other than inert or hazardous. The landfill covers 20 ha and 40 000 Mg of wastes is deposited annually. Municipal waste is not segregated at the landfill. The research was conducted in April, May and November 2012. Number of the psychrophilic and mesophilic bacteria and fungi was estimated by a culture-based method. Quantitative determination of sulfur compounds and meteorological and olfactrometric examinations were also carried out. Chemical analysis was conducted with a Photovac Voyager portable gas chromatograph. Air samples were collected at 5 points. The largest group of microbes were psychrophilic bacteria, especially in summer. The highest concentration of hydrogen sulfide and other odorants was found at leachate tank and landfill body. According to the Polish Standard for the assessment of atmospheric air pollution the air in the area of the landfill is classified as not contaminated and sporadically moderately contaminated. In spring and summer the number of microscopic fungi was increased also in control samples.


Helia ◽  
2019 ◽  
Vol 42 (71) ◽  
pp. 145-159
Author(s):  
V. A. Lyakh ◽  
N. I. Kostyuchenko ◽  
I. A. Shevchenko

Abstract The bacterial and micromycete complexes in the rhizosphere of sunflower plants non-infected and infected with broomrape (Orobanche cumana Wallr.) have been compared. The investigations were carried out in the conditions of a stationary infectious field which was annually enriched with infected plant residues and broomrape seeds collected in different regions of Ukraine. Soil is leached, low-humic chernozem with acidic pH. The soil samples selected at the end of vegetation from the rhizosphere of healthy and infected with broomrape plants of sunflower breeding samples. The total number of bacteria found in the rhizosphere of sunflower plants infected by the parasite did not differ significantly from the control and was 11.7 and 12.1 million CFU / g of soil, respectively. The numbers of ammonifiers as well as bacterial microflora, using for its life mineral nitrogen, and pedotrophs and oligotrophs in the compared soil samples did not differ significantly, and generally corresponded to this type of soil. Although in general, both tested samples of soil were characterized by a low content of bacteria of the genus Azotobacter, the number of representatives of this genus in the rhizosphere of parasite-infected plants was somewhat less than in control (35 % and 21 %, respectively). However, unlike most bacteria, the number of micromycetes detected on Czapek-Dox and starch-ammonia agar media, in the rhizosphere of plants infected by broomrape almost twice exceeded the number of these microorganisms in the rhizosphere of healthy plants. Analysis of the generic and species composition of microscopic fungi showed that in the rhizosphere of sunflower plants infected by the parasite a very specific mycocenosis was formed that differ from a mycocenosis of healthy plants. This mycocenosis was characterized by a much smaller number of genera and species of micromycetes. At the same time for the structure of the fungal cenosis of diseased plants there was a characteristic increase in the proportion of toxin-forming fungi of the Aspergillus and Penicillium genera with active conidiogenesis. The obtained data testify not only to the differences in the microbial complexes in the rhizosphere of sunflower plants non-infected and infected by broomrape, but also indicate the direction of action of this parasite.


Biologia ◽  
2015 ◽  
Vol 70 (7) ◽  
Author(s):  
Michał Hurej ◽  
Halina Kucharczyk ◽  
Jacek P. Twardowski ◽  
Andrzej Kotecki

AbstractThe aim of this study was to determine thrips abundance, species composition, sex ratio and seasonal dynamics of these insects on two morphological forms of Andean lupin (epigonal and semiepigonal). Thrips were studied in the lupine plants sown at two dates at the Experimental Research Station at Pawłowice, near Wrocław, Lower Silesia, Poland, during two growing seasons (2011-2012). The experiment was designed as a split-plot with four replicates for each morphological form and time of sowing. For the determination of thrips species’ composition, plants were collected from the central part of each plot when lupin was at the full blooming stage. In the laboratory, plants were shaken over a sheet of white paper. Once in each season thrips were also collected with a sweep net. In the laboratory larvae and adults were identified to species level and separated into males and females. During the whole lupin growing season, insects were also counted weekly on 10 consecutive plants in the middle row of each plot. Frankliniella intonsa, Thrips major and Aeolothrips intermedius were the dominant species in the full blooming stage of both semiepigonal and epigonal forms of Andean lupin. Thrips tabaci also occurred in greater numbers. There were no significant differences between numbers of thrips and number of species occurring on semiepigonal and epigonal plants sown at the same time. In the most numerous species, like F. intonsa and T. major, mainly females occurred in their population. In the population of A. intermedius males were abundant. Thrips occurring in Lupinus mutabilis, irrespective of the used form of lupin or sowing time, had one peak of abundance, i.e., the last ten days of June and the first ten days of July, when plants were at the blooming stage.


2014 ◽  
Vol 35 (1) ◽  
pp. 99-106
Author(s):  
Teresa Korniłłowicz-Kowalska ◽  
Alicja Szwed ◽  
Stefania Jezierska-Tys

Tobacco dust colonization by soil fungi involves the succession of physiologically differentiated groups. They are characterized by poorly diversified species composition and are dominated by potentially phytopathogenic forms.


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