scholarly journals Sensory atributes, nutricional values and innocuousness of hamburgers made with cherry pulp, soy and pork meet

2020 ◽  
Vol 38 (1) ◽  
pp. 128-150
Author(s):  
Nelson Loyola ◽  
Carlos Acuña ◽  
Mariela Arriola
Keyword(s):  
Lactic Acid ◽  
Soy Protein ◽  
Meat Products ◽  
Control Treatment ◽  
E Coli ◽  
Overall Acceptability ◽  
Sensorial Attributes ◽  
Sensorial Evaluation ◽  
Microbiological Aspects ◽  
Made In

The addition of pulps and extracts from some fruits has proved to be good to replace fat in food, aside from the addition oCof a higher nutritional value to chopped-meat products, such as hamburgers. In this work, hamburgers made with pork meet with the addition of cherry pulp and soy protein were assessed; physical, chemical and microbiological aspects were analyzed as well as their sensorial qualities. Three treatments with different percentages such as; pork meet 63.4 %, cherry pulp 8 %, soy protein 4.3 % and biosalt 1.1 % were made. Moisture was determined by the method (AOAC 950.46). In addition, pH and the percentage of lactic acid present in hamburgers was measured, presence of E. coli, as well as the sensorial evaluation according to their sensorial attributes and their overall acceptability. The content of moisture was not significantly different among the treatments (p≤ 0.05). Percentage of fat changed 30.5 % (T1) and 34.4 % (T3), being observed significant differences between both treatments (p≤ 0.05). The pH varied between 32 % (T1), 5.5 % (T2) and 23.7 % (T3) as compared with the hamburgers made in the control treatment T0, being significantly different from those made in treatments T1 and T2. The content of lactic acid showed an increase of 71.4 % (T1), 69.2 % (T2) and 36.4 % (T3), being significantly different among them. Significant differences (p≤ 0.05) in the perception of the attributes color and texture on the assessed hamburgers were detected, which did not happen with the attributes flavor and aroma. The microbiological evaluation, it was observed no presence of E.coli. The hypothesis of this study was not completely fulfilled because the addition of cherry pulp to make hamburgers effectively reduced the fat content, but the sensorial chemical aspects, acceptability and innocuousness did not show any improvement as compared to the control treatment.

scholarly journals Effect of the Addition of Soy Protein (Glycine Max Merryl) on the Composition of Cooked Ham

2021 ◽  
Author(s):  
E. Romero Machado ◽  
T. Zambrano Núñez ◽  
P. Pino Falconí
Keyword(s):  
Glycine Max ◽  
Soy Protein ◽  
Sensory Analysis ◽  
Laboratory Tests ◽  
Experimental Approach ◽  
Meat Products ◽  
Control Treatment ◽  
Microbiological Analysis ◽  
Randomized Design ◽  
Cooked Ham

The present study aimed to assess the bromatological composition when soy protein was added in the preparation of cooked ham. An experimental approach was used. There were three treatments in which 2.50% (T1), 5.00% (T2) and 7.50% (T3) soy protein was added; these were compared to a control treatment (T0). A randomized design was applied and means were analyzed according to Tukey at 5%. The following laboratory tests were carried out in the ham finished product: bromatological analysis, microbiological analysis and sensory analysis. The results obtained were as follows: fiber: 2.27%; humidity: 63.53%; fat: 7.47%; protein: 20.23%; ash: 1.83%; and carbohydrates: 4.68%. Significant differences were found between the addition of 7.50% of soy protein compared to the 0% control treatment. It can be concluded from the results that the addition of soy protein to ham increases the content of fiber, fat, protein, ash and carbohydrates, and the best treatment without affecting the characteristics of the finished product is the addition of 7.50% soy protein. Keywords: meat products, ham, soy protein. RESUMEN El presente estudio tiene como objetivo adicionar proteína de soya en la elaboración de jamón cocido y valorar la composición bromatológica. El método utilizado en la investigación es experimental manejando tres tratamientos en los cuales se añadió proteína de soya en 2,50 (T1), 5,00 (T2) y 7,50 (T3)%, que se los comparó a un tratamiento control (T0), se aplicó un diseño completamente al azar y separación de medias según Tukey al 5%, en el jamón como producto terminado, se realizaron las siguientes pruebas de laboratorio; análisis bromatológico, análisis sensorial.  Los resultados obtenidos de fibra (2,27%), humedad (63,53%), grasa (7,47%), proteína (20,23%), ceniza (1,83%) y carbohidratos (4,68%), se encontró diferencias significativas entre la adición de 7,50% de proteína de soya frente al tratamiento control 0%. Con los resultados se puede aludir que las formulaciones de jamón con adición de proteína de soya, aumenta los contenido de fibra, grasa, proteína, ceniza y carbohidratos  en relación a un tratamiento control, se puede establecer  que el mejor tratamiento es con la adición del 7,50% de proteína de soya sin que afecte las características del producto terminado Palabras claves: productos cárnicos, jamón, proteína de soya.

Presence of Bacterial Pathogens and Levels of Indicator Bacteria Associated with Duck Carcasses in a Commercial Processing Facility

2020 ◽  
Vol 83 (4) ◽  
pp. 605-608
Author(s):  
M. E. BERRANG ◽  
R. J. MEINERSMANN ◽  
S. W. KNAPP
Keyword(s):  
Meat Products ◽  
Aerobic Bacteria ◽  
E Coli ◽  
Before And After ◽  
Processing Line ◽  
Commercial Processing ◽  
Processing Techniques ◽  
Sampling Times ◽  
Live Bird ◽  
Microbiological Aspects

ABSTRACT Little information has been published on the microbiological aspects of U.S. commercial duck processing. The objective of this study was to measure prevalence and/or levels of bacteria in duck samples representing the live bird and partially or fully processed oven-ready duck meat. At 12 monthly sampling times, samples were collected at six sites along the processing line in a commercial duck slaughter plant. Crop and cecum samples were collected at the point of evisceration. Whole carcass rinse samples were collected before and after carcass immersion chilling plus application of an antimicrobial spray. Leg quarters were collected from the cut-up line before and after application of an antimicrobial dip treatment. All samples (five from each site per monthly replication) were directly plated and/or enriched for Salmonella and Campylobacter. For the last 10 replications, carcass and leg quarter rinse samples were also evaluated for enumeration of total aerobic bacteria, Escherichia coli, and coliforms. Most cecum, crop, and prechill carcass rinse samples were positive for Campylobacter (80, 72, and 67%, respectively). Carcass chilling and chlorinated spray significantly lowered Campylobacter prevalence (P < 0.01), and even fewer leg quarters were positive for Campylobacter (P < 0.01). Passage through a chlorinated dip did not further reduce Campylobacter prevalence on leg quarters. Salmonella was infrequently found in any of the samples examined (≤10%). Total aerobic bacteria, coliforms, and E. coli levels were reduced (P < 0.01) on whole carcasses by chilling but were not different after cut-up or leg quarter dip treatment. Overall, current commercial duck processing techniques as applied in the tested plant were effective for reducing the prevalence and levels of Campylobacter on duck meat products. HIGHLIGHTS

scholarly journals Acidifier alami air perasaan jeruk nipis (Citrus aurantiun) sebagai pengganti antibiotik growth promotor ayam broiler

2018 ◽  
Vol 16 (2) ◽  
pp. 27
Author(s):  
K Hidayat ◽  
S Wibowo ◽  
L A Sari ◽  
A Darmawan
Keyword(s):  
Drinking Water ◽  
Lactic Acid ◽  
Broiler Chickens ◽  
Lemon Juice ◽  
Feed Consumption ◽  
Control Treatment ◽  
Growth Promoter ◽  
Feed Conversion ◽  
Lime Juice ◽  
E Coli

ABSTRAK<br /><br />Penelitian ini bertujuan untuk mengevaluasi pemberian air jeruk nipis dalam air minum sebagai pengganti antibiotic growth promotor terhadap performa dan populasi mikroba usus halus ayam broiler. Ayam broiler yang digunakan dalam penelitian ini yaitu 90 ekor ayam broiler dipelihara dari umur 1 hari hingga umur 28 hari. Penelitian ini menggunakan rancangan acak lengkap dengan 3 perlakuan dan 3 ulangan. Perlakuan ini yaitu P0= Ransum + air minum tanpa perasan air jeruk nipis (kontrol), P1= Ransum + air minum dengan perasan air jeruk nipis hingga pH air minum menjadi 5, P2= Ransum + air minum dengan perasan air jeruk nipis hingga pH air minum menjadi 3. Peubah yang diamati dalam penelitian ini yaitu konsumsi pakan, pertambahan bobot badan, konversi pakan, populasi bakteri E. coli, populasi bakteri asam laktat, dan mortalitas. Hasil penelitian ini menunjukkan bahwa pemberian air perasan jeruk nipis dalam air minum tidak memberikan efek berbeda nyata pada pertambahan bobot badan, konsumsi ransum, konversi ransum, populasi E. coli, akan tetapi perlakuan memberikan efek berbeda nyata (P&lt;0.05) pada populasi bakteri asam laktat yang terdapat pada digesta usus halus ayam broiler. Pemberian perasaan air jeruk nipis dapat meningkatkan efisiensi pakan sebesar 3,3 %.<br /><br />Kata kunci : Ayam broiler, acidifier, jeruk nipis, performa, E. coli, bakteri asam laktat<br /><br />ABSTRACT<br /><br />The purpose of this study was to evaluate the effect of lime juice in drinking water as antibiotic growth promoter substitute on broiler performance and intestine microbial population. This study used 90 broiler chickens that were reared up to 28 days. This experiment was designed as a completely randomized design with 3 treatments and 3 replications; P0= Diet + drinking water without lime juice (control treatment), P1= Diet + drinking water with lemon juice (pH=5), P2= Diet + drinking water with lime juice (pH =3). The results of this study showed that all treatments did not affect weight gain, feed consumption, feed conversion, E. coli population. The treatment of drinking water with lime juice at pH =3 significantly increased (P&lt;0.05) lactic acid population. Utilization of lime juice in drinking water at pH 5 increase 3,3% of feed efficiency and reduce the mortality rate.<br /><br />Key words: lime juice, broiler perfomance, lactic acid bacteria

The effect of a biological silage additive and type of supplement on the performance of beef cattle

1990 ◽  
Vol 1990 ◽  
pp. 138-138
Author(s):  
R Laird ◽  
A Gill ◽  
J T McAuslan ◽  
C Thomas ◽  
I D Wright
Keyword(s):  
Trace Elements ◽  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
The Other ◽  
Control Treatment ◽  
Pediococcus Acidilactici ◽  
Mineral Salts ◽  
Feeding Value ◽  
Additive Control ◽  
Made In

The biological silage additive, Safe-Sile, is a commercial product prepared by Microferm Ltd. and the objective of the experiment was to appraise its efficacy in the conservation of silage and its subsequent feeding value.Safe-sile contains lactic acid bacteria (2.5 x 109 cfu/g consisting of proportionately 0.75 Lactobacillus plantarum and 0.25 Pediococcus acidilactici) together with enzymes (hemicellulase, amyloglucosidase and cellulase) and a mixture of bacteriophages (2.5 x 109 pfu/g). These active ingredients are carried in dextrose together with mineral salts, trace elements and vitamins.Two silages were made in September 1988 from third-cut wilted grass, one silage receiving no additive (control treatment) and the other receiving Safe-Sile at the rate of 1 kg per 15 tonnes of grass approximately. The fields harvested for this experiment were perennial ryegrass/white clover swards and the silages were made after a field wilting period of 24 hours under good silage-making conditions.

Validation of Lactic Acid Bacteria, Lactic Acid, and Acidified Sodium Chlorite as Decontaminating Interventions To Control Escherichia coli O157:H7 and Salmonella Typhimurium DT 104 in Mechanically Tenderized and Brine-Enhanced (Nonintact) Beef at the Purveyor

2010 ◽  
Vol 73 (12) ◽  
pp. 2169-2179 ◽  
Author(s):  
ALEJANDRO ECHEVERRY ◽  
J. CHANCE BROOKS ◽  
MARKUS F. MILLER ◽  
JESSE A. COLLINS ◽  
GUY H. LONERAGAN ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Salmonella Typhimurium ◽  
Control Point ◽  
Meat Products ◽  
Sodium Chlorite ◽  
Escherichia Coli O157 ◽  
E Coli ◽  
Critical Control Point

After three different outbreaks were linked to the consumption of nonintact meat products contaminated with Escherichia coli O157:H7, the U.S. Food Safety and Inspection Service published notice requiring establishments producing mechanically tenderized and moisture-enhanced beef products to reassess their respective hazard analysis and critical control point systems, due to potential risk to the consumers. The objective of this study was to validate the use of lactic acid bacteria (LAB), acidified sodium chlorite (ASC), and lactic acid (LA) sprays when applied under a simulated purveyor setting as effective interventions to control and reduce E. coli O157:H7 and Salmonella Typhimurium DT 104 in inoculated U.S. Department of Agriculture (USDA) Choice strip loins (longissimus lumborum muscles) pieces intended for either mechanical blade tenderization or injection enhancement with a brine solution after an aging period of 14 or 21 days at 4.4°C under vacuum. After the mechanical process, translocation of E. coli O157:H7 and Salmonella Typhimurium DT 104 from the surface into the internal muscles occurred at levels between 1.00 and 5.72 log CFU/g, compared with controls. LAB and LA reduced internal E. coli O157:H7 loads up to 3.0 log, while ASC reduced the pathogen 1.4 to 2.3 log more than the control (P &lt; 0.05), respectively. Salmonella Typhimurium DT 104 was also reduced internally 1.3 to 2.8, 1.0 to 2.3, and 1.4 to 1.8 log after application of LAB, LA, and ASC, respectively. The application of antimicrobials by purveyors prior to mechanical tenderization or enhancement of steaks should increase the safety of these types of products.

Effectiveness of Spraying with Tween 20 and Lactic Acid in Decontaminating Inoculated Escherichia coli O157:H7 and Indigenous Escherichia coli Biotype I on Beef†‡

2002 ◽  
Vol 65 (1) ◽  
pp. 26-32 ◽  
Author(s):  
MEHMET CALICIOGLU ◽  
CHARLES W. KASPAR ◽  
DENNIS R. BUEGE ◽  
JOHN B. LUCHANSKY
Keyword(s):  
Escherichia Coli ◽  
Lactic Acid ◽  
Control Treatment ◽  
Separate Experiment ◽  
Tween 20 ◽  
Escherichia Coli O157 ◽  
E Coli ◽  
Significant Difference ◽  
Peptone Water ◽  
Manure Slurry

Beef carcass quarters and fat-covered subprimal cuts were suspended vertically and inoculated with a bovine manure slurry containing a five-strain mixture of Escherichia coli O157:H7 to deliver about 4 to 5 log10 CFU/cm2. To identify treatments that would improve the effectiveness of spraying with lactic acid (LA), the inoculated quarters and cuts were treated as follows: experiment A, (i) not treated (control), (ii) sprayed with 2% (vol/vol) LA, (iii) tempered at 21°C for 4 h, and (iv) tempered and then sprayed with LA; experiment B, (v) sprayed with water, (vi) sprayed with LA, (vii) sprayed with LA containing 0.5% (vol/vol) sodium benzoate (SB), and (viii) sprayed with LA containing SB and 5% (vol/vol) Tween 20 (TW20); and experiment C, (ix) sprayed with water (no prespray), (x) presprayed with TW20 and then sprayed with LA, and (xi) presprayed with TW20 and then sprayed with LA containing SB. In experiment A, spraying carcasses with LA significantly (P &lt; 0.05) reduced the numbers of E. coli Biotype I and serotype O157:H7 after 1 and 3 days of storage, respectively. The tempering process employed did not affect the effectiveness of the LA spray on either type of E. coli. In experiment B, there was no significant difference in the reduction of E. coli O157:H7 on subprimal cuts sprayed with water and that on cuts sprayed with LA alone or with LA in combination with SB and TW20 after 1 or 3 days of storage (total reductions ranged from about 1.6 to 2.8 log10 CFU/cm2). In experiment C, prespraying subprimal cuts with TW20 significantly (P &lt; 0.05) increased the effectiveness of LA (reductions of 2.8 and 3.2 log10 CFU/cm2, respectively) and that of LA with SB (reductions of 2.6 and 3.3 log10 CFU/cm2, respectively) compared with spraying with water alone (reductions of ca. 1.0 and 2.0 log10 CFU/cm2, respectively) after 1 and 3 days of storage, respectively. In a separate experiment, the incorporation of TW20 (0.1 or 0.25%) into buffered peptone water prior to the maceration of excised carcass surface samples resulted in the recovery of significantly larger numbers (ca. 5.1 to 5.2 log10 CFU/cm2) of E. coli O157:H7 cells than did the control treatment without added TW20 (ca. 3.8 to 4.6 log10 CFU/cm2). These results demonstrate that the treatment of beef carcasses with LA reduces the number of viable E. coli O157:H7 cells and that this inactivation or removal by LA is enhanced by prespraying of the carcass with a 5% solution of TW20.

scholarly journals Effect of Lactic Acid Bacteria Addition on the Microbiological Safety of Pasta-Filata Types of Cheeses

2020 ◽  
Vol 11 ◽  
Author(s):  
Alžbeta Medved’ová ◽  
Martina Koňuchová ◽  
Karolína Kvočiková ◽  
Ivana Hatalová ◽  
L’ubomír Valík
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Starter Culture ◽  
Raw Milk ◽  
Inhibitory Effect ◽  
E Coli ◽  
Overall Acceptability ◽  
Commission Regulation ◽  
Negative Effect ◽  
Pasta Filata

In this work, the effects of different combinations of lactic acid bacteria (LAB) on the growth of coagulase-positive staphylococci (CPS) and Escherichia coli were evaluated during ripening of 23 curd cheeses, and their subsequent behavior during the manufacture and storage of pasta-filata cheeses was characterized. Three groups of cheeses were prepared in total: first, control cheeses from raw milk without LAB addition; further pasteurized milk cheeses with LAB, CPS and E. coli intentional inoculation; and finally, raw milk cheeses with LAB added. The aim was to compare the effect of LAB from starter culture, and also in combination with native LAB, and to evaluate the LAB effect as a group, and further to suggest the culture with the best inhibitory potential. Based on the results, counts of CPS increased over 24 h in control curd cheese by 1.76 ± 0.56 log CFU/g. On the other hand, in raw milk cheeses with the addition of starter culture, the increase in CPS counts by 0.76 ± 0.87 log CFU/g was noticed. Counts of E. coli increased during the first 24 h of curd manufacture by 3.56 ± 0.41 log CFU/g in cheeses without LAB addition. Contrary to this, using of LAB cultures resulted in an increase in E. coli counts by only 1.40 ± 1.07 log CFU/g. After steaming at 63.6 ± 1.9°C for 7.2 ± 2.1 min (temperature of heated acidified curd was 54.9 ± 1.7°C), CPS decreased by 0.58 ± 1.12 log CFU/g, and E. coli decreased by 1.23 ± 0.97 log CFU/g in all cheeses, regardless of LAB addition. Finally, during storage of cheeses at 6 ± 0.5°C for 28 days, the levels of E. coli in control cheeses and in raw milk LAB-enriched cheeses reached levels of 2.07 ± 2.28 log CFU/g and 1.20 ± 0.85 log CFU/g, respectively. In addition, the counts of CPS at the end of storage met the criteria of EU Commission Regulation (EC) No. 1441/2007 (2007) (less than 4 log CFU/g) in all manufactured cheeses with added LAB culture, while in the control raw milk cheeses, a level of 3.80 ± 1.22 log CFU/g was observed. Regarding the culture used, the best microbiological inhibitory effect in 28-day-old cheeses was reached by the combination of Fresco culture with Lacticaseibacillus rhamnosus GG, and the best sensory properties were judged to be those for cheeses manufactured with Culture A. A moderate negative effect of storage on overall sensory acceptance was noted, according to the final evaluation of overall acceptability of pasta-filata cheeses. The most satisfactory overall acceptability after 28 days of storage at 6°C was reached for cheese with the addition of culture A.

scholarly journals Physical-Chemical and Microbiological Analysis of the Steaming Brine Used in the Fabrication of Pressed Cheeses

1970 ◽  
Vol 66 (2) ◽  
Author(s):  
Dorin ŢIBULCĂ ◽  
Aurora ŢIBULCĂ ◽  
Mirela JIMBOREAN ◽  
Cornel LASLO ◽  
Delia TRUŢĂ
Keyword(s):  
Salt Concentration ◽  
Coliform Bacteria ◽  
Microbiological Analysis ◽  
Salmonella Spp ◽  
E Coli ◽  
Milk Processing ◽  
Physical Chemical ◽  
Microbiological Aspects ◽  
Made In

The investigations had as aim to study the physical-chemical aspects (salt concentration and temperature) and microbiological aspects (TNG, coliform bacteria, E. coli, Stqfilococcus aureus c.p. and Salmonella spp.) of the steaming brine in the fabrication of the pressed cheeses Dalia, Rucăr, Penteleu. The investigations were made in five milk processing units in cheeses with steamed paste.

scholarly journals Microbiological hazards at several stages of production and distribution of cooked sausages

2018 ◽  
Vol 53 (3) ◽  
pp. 201
Author(s):  
D. SERGELIDIS (Δ. ΣΕΡΓΚΕΛΙΔΗΣ) ◽  
A. ABRAHIM (Α. AMIN) ◽  
A. SARIMVEI (Α. ΣΑΡΗΜΒΕΗ) ◽  
C. GENIGEORGIS (Κ. ΓΕΝΗΠΩΡΓΗΣ)
Keyword(s):  
Lactic Acid ◽  
Thermal Treatment ◽  
Lactic Acid Bacteria ◽  
Pathogenic Bacteria ◽  
Meat Products ◽  
Salmonella Spp ◽  
E Coli ◽  
Spoilage Bacteria ◽  
Cooked Meat ◽  
Cooked Meat Products

Fifty one (51) samples of several types of cooked sausage paste, prepared by two meat factories in N. Greece were examined. TPC of these samples ranged between 5,3-6,3 Log10CFU/g. Coliforms were regularly present reaching populations of 93->2.400 MPN/g and lactic acid bacteria ranged between 5-6,3 Log10CFU/g. L. monocytogenes was detected in 56 and 38,4% of the samples collected in each factory. E. coli was detected in 20 and 16,6%, and Salmonella spp in 12 and 16,6% respectively. Neither pathogens nor coliforms were detected in 51 samples of cooked sausages originated from the same pastes examined before. No recovery of any injured cells of the pathogenic bacteria and coliforms was observed after their storage at 4°C for 20 days. TPC of the cooked sausage samples, after thermal treatment, ranged between 3-4,7 Log10CFU/g and consisted mainly of lactic acid bacteria (range <2-4,5 Log10CFU/g) and sporeformers (range 3-4,5 Log10CFU/g). After 20 days storage at 4°C the TPC and lactid acid bacteria counts of the cooked sausages, increased by <1 Log. We also examined 16 surface and center samples of cooked sausages and meat products without casings, consisting of big meat pieces (bacon, smoked ham,etc). Surface TPC ranged between 5-5,3 Log10CFU/g and from the center of the meats they ranged between 2-3,5 Log1 0CFU/g. Coliforms, E. coli, L. monocytogenes and Salmonella spp were not detected. Lactic acid bacteria were the main flora. Furthermore we examined surface samples of cooked meat products, without casings, during several stages following thermal treatment and up to storage for 24 h at 4° C, without any protective package. TPC immediately after thermal treatment were <2 Log^CFU/g, after cooling with water increased they increased at 3 Log10CFU/g and remained the same during the following 24 h storage at 4°C. Coliforms were detected in the stored products. Their populations exceeded 2.400 MPN/g on the surface of the samples after storage for a few days at 4°C. It is assumed that the flora on the surface of these products originated from the environment and the cooling water. Finally we examined 69 samples from surfaces of the slicing and packaging equipment of cooked meat products in 3 meat factories and 28 samples from 12 super markets. L. monocytogenes was detected in 6 and 14,2% of the samples that originated from the slicing blades in the factories and super markets respectively. The results of this study underline the importance of GMP for the prevention of contamination of cooked sausages with pathogens and the control of the growth of the spoilage bacteria population which minimize the self life of these products. This is especially true after thermal treatment during peeling and slicing.

scholarly journals Multiresistant Bacteria Isolated from Intestinal Faeces of Farm Animals in Austria

Antibiotics ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 466
Author(s):  
Herbert Galler ◽  
Josefa Luxner ◽  
Christian Petternel ◽  
Franz F. Reinthaler ◽  
Juliana Habib ◽  
...  
Keyword(s):  
Meat Products ◽  
Animal Husbandry ◽  
Multidrug Resistant ◽  
Farm Animals ◽  
Resistant Bacteria ◽  
Antibiotic Resistant Bacteria ◽  
Antibiotic Resistant ◽  
E Coli ◽  
Multiresistant Bacteria ◽  
Vancomycin Resistant

In recent years, antibiotic-resistant bacteria with an impact on human health, such as extended spectrum β-lactamase (ESBL)-containing Enterobacteriaceae, methicillin-resistant Staphylococcus aureus (MRSA) and vancomycin-resistant Enterococci (VRE), have become more common in food. This is due to the use of antibiotics in animal husbandry, which leads to the promotion of antibiotic resistance and thus also makes food a source of such resistant bacteria. Most studies dealing with this issue usually focus on the animals or processed food products to examine the antibiotic resistant bacteria. This study investigated the intestine as another main habitat besides the skin for multiresistant bacteria. For this purpose, faeces samples were taken directly from the intestines of swine (n = 71) and broiler (n = 100) during the slaughter process and analysed. All samples were from animals fed in Austria and slaughtered in Austrian slaughterhouses for food production. The samples were examined for the presence of ESBL-producing Enterobacteriaceae, MRSA, MRCoNS and VRE. The resistance genes of the isolated bacteria were detected and sequenced by PCR. Phenotypic ESBL-producing Escherichia coli could be isolated in 10% of broiler casings (10 out of 100) and 43.6% of swine casings (31 out of 71). In line with previous studies, the results of this study showed that CTX-M-1 was the dominant ESBL produced by E. coli from swine (n = 25, 83.3%) and SHV-12 from broilers (n = 13, 81.3%). Overall, the frequency of positive samples with multidrug-resistant bacteria was lower than in most comparable studies focusing on meat products.

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