Effect of Honey on Orthodontic Tooth Movement and Osteoclastic Activity in Psychologically Stressed Animals

Introduction: Orthodontics is a clinical specialty in dentistry related to the correction of dentofacial deformities. Psychological stress factors delay Orthodontic tooth movement (OTM). Honey can be considered a useful and harmless natural product to reduce stress levels, hence improves the efficacy of OTM. Aims & Objectives: To compare the differences in the rate of tooth movement and osteoclastic activity between control, psychologically stressed and honey treated psychologically stressed groups after 1 week of orthodontic force application in an animal model. Place and duration of study: This experimental study was conducted at the animal research laboratory and Histopathology Department of Post Graduate Medical Institute (PGMI), Lahore, Pakistan, from April 2019 to June 2020. Material & Methods: Thirty-six Sprague Dawley rats were randomly divided into A, B and C groups. Psychological Stress was induced in group A (PS group) while Honey was given orally as a therapeutic agent along with induction of psychological stress in group B (PSH group), and group C was the Control Group. Statistical analyses were performed using SPSS version 24 software. The quantitative variables were the orthodontic tooth movement, the osteoclast count, and the expression of RANKL. One-way ANOVA was applied to calculate the mean difference and Post hoc Tukey test applied for multiple comparisons among the groups. A p-value ? 0.05 was considered statistically significant in all 3 groups. Results: There was a significant difference (p-value <0.05) between control and experimental groups in the orthodontic tooth movement and levels of RANKL, however, there was no significant difference between PS and PSH groups. Conclusion: Psychological stress delays orthodontic tooth movement by causing a reduction in its rate and osteoclastic activity and honey has no significant correlation with lowering stress levels, hence does not improve orthodontic tooth movement efficiency.

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Combined Effect of Nicotine and Caffeine on Orthodontic Tooth Movement in Rats

Journal of Islamabad Medical & Dental College ◽

10.35787/jimdc.v9i2.462 ◽

2020 ◽

Vol 9 (2) ◽

pp. 109-114

Author(s):

NAEEM MURTAZA ◽

WAHEED UL HAMID ◽

AHMAD SHAMIM ◽

SHABBIR HUSSAIN ◽

Muhammad Azeem ◽

...

Keyword(s):

Tooth Movement ◽

Orthodontic Tooth Movement ◽

Combined Effect ◽

Medical Institute ◽

Control Group ◽

P Value ◽

Sprague Dawley ◽

Sprague Dawley Rats ◽

The Mean ◽

Animal House

Background: The individual effects of nicotine and caffeine have been reported in previous studies but their combined effect on tooth movement needs to be elucidated. The objective of this study was to evaluate the combined effect of nicotine and caffeine on the magnitude of orthodontic tooth movement (OTM) in rats. Material and Methods: This experimental study was conducted on Sprague-Dawley rats (Animal House and Pathology Laboratory; Post Graduate Medical Institute, Lahore) in the department of Orthodontics, de’Montmorency College of Dentistry, Lahore from 8th July 2014 to 8th January 2015. Forty male Sprague-Dawley rats were divided into four equal groups: Control group (CR), nicotine group (NT), caffeine group (CF) and combined nicotine and caffeine group (CNC). Closed coil nickel titanium (NiTi) spring was placed between incisor and maxillary molar. Nicotine group (NT) was treated by intraperitoneal injections of nicotine. Caffeine was given to caffeine group and Combined nicotine and caffeine group (CNC) was treated in the same way as individual nicotine and caffeine groups daily for 14 days. All the rats were sacrificed on 15th day. Magnitude of the orthodontic tooth movement was measured using digital Vernier caliper. Means and standard deviation were calculated for orthodontic tooth movement. One-way ANOVA was used to determine the mean difference in OTM. Post hoc Tukey test was used for multiple comparisons among the groups. Results: The mean orthodontic tooth movement (OTM) was 0.32 mm ± 0.05 in control group, 0.56 mm ± 0.04 in nicotine group, 0.52 mm ± 0.034 in caffeine group and 0.8 mm ± 0.06 in combined NC group, respectively. The difference between mean OTM among the groups was statistically significant (P-value <0.001). The mean OTM in CNC group was significantly higher as compared to other groups (CR, NT, CF, NT) (P-value <0.001). Conclusions: In rats, the combined use of nicotine and caffeine results in greater orthodontic tooth movement as compared to their individual use.

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The effect of autologous leukocyte platelet rich fibrin on the rate of orthodontic tooth movement: A prospective randomized clinical trial

European Journal of Dentistry ◽

10.4103/ejd.ejd_424_17 ◽

2018 ◽

Vol 12 (03) ◽

pp. 350-357 ◽

Cited By ~ 4

Author(s):

Azita Tehranchi ◽

Hossein Behnia ◽

Fereydoun Pourdanesh ◽

Parsa Behnia ◽

Nelson Pinto ◽

...

Keyword(s):

Clinical Trial ◽

Tooth Movement ◽

Random Effect ◽

Random Effect Model ◽

Orthodontic Tooth Movement ◽

Control Group ◽

Extraction Socket ◽

Effect Model ◽

Significant Difference ◽

And Control

ABSTRACT Objective: The aim of this study was to evaluate the effect of LPRF, placed in extraction sockets, on orthodontic tooth movement (OTM). Materials and Methods: Thirty extraction sockets from eight patients (five males, three females, with a mean age of 17.37 years; range 12–25 years) requiring extraction of first premolars based on their orthodontic treatment plan participated in this split-mouth clinical trial. In one randomly selected quadrant of each jaw, the extraction socket was preserved as the experimental group by immediate placement of LPRF in the extraction socket. The other quadrant served as the control group for secondary healing. Immediately, the teeth adjacent to the defects were pulled together by a NiTi closed-coil spring with constant force. A piece of 0.016 × 0.022-inch stainless steel wire was used as the main arch wire. The amount of OTM was measured on the study casts at eight time points with 2-week intervals for 3 months. Analysis of random effect model was performed for the purpose of comparison between the experimental and control groups. Results: According to the random effect model, a statistically significant difference was found between the experimental and control group in rate of OTM (P = 0.006). Conclusion: According to the results, application of LPRF, as an interdisciplinary approach combining orthodontics and surgery, may accelerate OTM, particularly in extraction cases.

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Comparison of Rate of Canine Retraction and Secondary Outcomes Associated With Conventional Fixed Orthodontic Treatment and Minimally Invasive Techniques (MOPs): A Randomized Control Trial

The Journal of Indian Orthodontic Society ◽

10.1177/0301574220963502 ◽

2020 ◽

pp. 030157422096350

Author(s):

Ajit Vikram Parihar ◽

Shivam Verma ◽

TP Chaturvedi ◽

Naresh Kumar ◽

A Kavin Prasanth ◽

...

Keyword(s):

Orthodontic Treatment ◽

Tooth Movement ◽

Root Resorption ◽

Control Group ◽

P Value ◽

Pain Level ◽

Canine Retraction ◽

Significant Difference ◽

Secondary Outcomes ◽

Anchorage Loss

Introduction: The objective of the study was to assess the rate of canine retraction and secondary outcomes associated with conventional fixed orthodontic treatment (CFO) and CFO with micro-osteoperforation (CFO + MOP), that is, anchorage loss, root resorption, vitality of tooth, pain and discomfort level during the procedure. Methods: A total of 16 patients with Class II Division 1 malocclusion who required upper first premolar extraction with lower non-extraction/single incisor extraction were divided into the test group (MOP) and positive control group (CFO + MOP) for a split-mouth study. Both maxillary canines were retracted with nickel–titanium (NiTi) closed coil springs. Patients were reviewed after 24 hours, 7 days, 28 days, and 4, 8, and 16 weeks to assess the rate of tooth movement, anchorage loss, root resorption, vitality of tooth, pain and discomfort level. Results: There was a statistically significant difference in the rate of tooth movement between the CFO and CFO + MOP groups after the first 4 weeks ( P-value = .026), whereas no statistically significant difference was observed at 8, 12, and 16 weeks ( P-value = .33, .99, and .08, respectively). In the CFO group, there was no statistically significant difference in tooth movement between different time intervals ( P-value > .05). There was no significant difference in root resorption between the groups. The pain level was higher in the MOP group in the first 24 hours ( P-value < .05) after the procedure. Later on, the difference in pain level between the groups was not significant ( P-value > .05). The vitality of retracted canines in both groups was healthy. Conclusion: The study recommends that the CFO + MOP procedure has substantial potential to be used as an adjunct to the routine mechanotherapy for faster tooth movement, as it may reduce the treatment time by half in the first 4 weeks after the MOP procedure. There are no potential differences in anchorage loss, tipping, vitality, and apical and lateral root resorption between the CFO and CFO + MOP groups. This trial was registered at Clinical Trial Registry, India.

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Pain Experience with Micro-osteoperforations during Initial Orthodontic Alignment: A Randomized Clinical Trial.

Journal Of Oral Research ◽

10.17126/joralres.2020.072 ◽

2020 ◽

Vol 9 (4) ◽

pp. 309-318

Author(s):

Azaitun Akma Shahrin ◽

◽

Sarah Haniza Abdul Ghani ◽

Noraina Hafizan Norman ◽

◽

...

Keyword(s):

Clinical Trial ◽

Repeated Measures ◽

Pain Perception ◽

Tooth Movement ◽

Intervention Group ◽

Orthodontic Tooth Movement ◽

Control Group ◽

Initial Alignment ◽

Significant Difference ◽

Fixed Appliances

Objetive: The objective of this clinical trial was to investigate the perception of pain during initial maxillary alignment with an adjunctive procedure of micro-osteoperforations (MOPs) compared to conventional orthodontics. Material and methods: This study design was a single-centre, two-arm parallel prospective randomised clinical trial. Thirty consecutive adult subjects (25 females and 5 males; mean age ± SD, 22.66 ± 3.27 years) with 5-8mm moderate upper labial segment crowding were randomly allocated using block randomisation into intervention and control group. All subjects had first premolar extractions, bonded conventional fixed appliances and 0.014-inch nickel-titanium archwire was placed for initial alignment. The intervention group received a 3-mm deep MOPs procedure under local anaesthesia using a Propel device (PROPEL Ortho Singapore) on the labiogingival aspect between the maxillary incisors. Both groups received a set of 100 mm visual analogue scale to complete over the first week, recording pain at 24 hours, 3 days and 1 week. Data were analysed using repeated-measures analysis of variance (ANOVA). Results: There was a statistically significant difference observed in perceived pain levels between MOPs and the control group on day 1, day 3 and day 7 postoperatively. Pain perception was significantly lower in the intervention group at all time points. Conclusion: Accelerating orthodontic tooth movement with MOPs did not accentuate pain perceived during initial maxillary alignment with fixed appliances.

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Effects of salbutamol, montelukast and prednisone on orthodontic tooth movement in rats

Brazilian Dental Science ◽

10.14295/bds.2021.v24i2.2394 ◽

2021 ◽

Vol 24 (2) ◽

Author(s):

Victor Chumpitaz-Cerrate ◽

Lesly Chávez-Rimache ◽

César Franco-Quino ◽

Eliberto Ruiz-Ramirez ◽

Elías Aguirre-Siancas ◽

...

Keyword(s):

Clinical Evaluation ◽

Tooth Movement ◽

Orthodontic Tooth Movement ◽

Study Groups ◽

Preclinical Study ◽

Control Group ◽

Histopathological Analysis ◽

Significant Difference ◽

Interincisal Distance

Objective: To evaluate the effect of salbutamol, montelukast, and prednisone on orthodontic tooth movement in rats. Material and Methods: In vivo experimental preclinical study. The sample consisted of 48 rats randomly distributed in four study groups. Group A was given saline solution; to group B, salbutamol 4 mg/Kg; to group C, montelukast 2.5 mg/Kg and to group D, prednisone 2.5 mg/Kg. All were fitted with orthodontic devices and the medications were administered intraperitoneally every 12 hours for 5 days. The clinical evaluation (variation in the interincisal distance) was performed at one, three, five, and seven days and the histopathological analysis (cell count) at five and seven days. Results: In the clinical evaluation of the variation in the interincisal distance, a significant difference was found in all the evaluations (p <0.05). It was found that the salbutamol group presented higher variation values in the interincisal distance on all the days evaluated. In the histopathological analysis at five and seven days, it was found that the osteoblast and osteocyte count was significantly higher in the salbutamol group compared to the other groups (p ><0.05). However, in the subgroup analysis, it was found that there was no significant difference in the osteoblast and osteocyte count between the prednisone, montelukast, and control group (p>0.05). Conclusion: The administration of salbutamol increased the magnitude of orthodontic tooth movement; nonetheless, the administration of montelukast and prednisone did not modify the magnitude of orthodontic tooth movement in rats. Keywords Albuterol; Montelukast; Prednisone; Rats; Tooth movement.

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Effects of 405-, 532-, 650-, and 940-nm wavelengths of low-level laser therapies on orthodontic tooth movement in rats

Progress in Orthodontics ◽

10.1186/s40510-020-00343-3 ◽

2020 ◽

Vol 21 (1) ◽

Author(s):

Hasibe Baser Keklikci ◽

Ahmet Yagci ◽

Arzu Hanim Yay ◽

Ozge Goktepe

Keyword(s):

Tooth Movement ◽

Orthodontic Tooth Movement ◽

Positive Control ◽

Control Group ◽

Bone Area ◽

Low Level ◽

Low Level Laser ◽

Level Laser ◽

Significant Difference ◽

532 Nm

Abstract Background Investigating the effects of 405-nm, 532-nm, 650-nm, and 950-nm wavelengths of LLLTs (low-level laser therapies) on the orthodontic tooth movement in rats by using histological and immunohistochemical methods. Forty-five Wistar albino rats were randomly divided into 5 groups: control group (positive control: the left maxillary 1st molar side; negative control: the right maxillary 1st molar side), 405 nm LLLT group (Realpoo), 532 nm LLLT group (Realpoo), 650 nm LLLT group (Realpoo), and 940 nm LLLT group (Biolase). The left maxillary 1st molar teeth of all rats were applied mesially 50-g force. Starting from the 1st day, 48 h intervals, LLLT was applied in continuous wave mode and in contact with the tissue. The application area was approximately 1 cm2. The lasers were performed for 3 min on each surface (buccal, palatal, mesial), totally 9 min (total dose 54 J/cm2). The amount of the molar mesialization, the bone area between the roots, PDL (periodontal ligament) measurements, TRAP (tartrate-resistant acid phosphatase), and ALP (alkaline phosphatase) immunoreactivity intensity were calculated. Results The amount of the molar mesialization was significantly higher in the 650 nm LLLT group (mean 0.878 ± 0.201 mm; 95% CI (confidence interval) 0.724 and 1.032) than in the groups of positive control (mean 0.467 ± 0.357 mm; 95% CI 0.192 and 0.741) and 405 nm LLLT (mean 0.644 ± 0.261 mm; 95% CI 0.443 and 0.845) (p < 0.001). There were significant differences in the PDL-mesial (p = 0.042) and PDL-distal (p = 0.007) regions between the groups. The immunoreactivity intensity for TRAP-mesial was significantly higher in the positive control group (mean 109,420.33 ± 8769.17; 95% CI 100,217.65 and 118,623.02) than in the 405 nm (mean 91,678.83 ± 7313.39; 95% CI 84,003.9 and 99,353.77) and the 650 nm LLLT (mean 87,169.17 ± 4934.65; 95% CI 81,990.56 and 92,347.77) groups (p = 0.002). There was no statistically significant difference between the groups on immunoreactivity intensity with ALP staining. Conclusions The results of this study show that LLLT with 650-nm wavelength increases orthodontic tooth movement more than 405-nm, 532-nm, and 940-nm LLLTs. The 940-nm and 650-nm LLLTs also increase the bone area between the roots by more than 405-nm and 532-nm wavelengths.

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The effect of red wine extract, resveratrol, on the degree and rate of orthodontic tooth movement in guinea pigs

APOS Trends in Orthodontics ◽

10.4103/2321-1407.163416 ◽

2015 ◽

Vol 5 ◽

pp. 181-189

Author(s):

Isidro Alex C. Urriquia ◽

Lotus D. Llavore

Keyword(s):

Guinea Pigs ◽

Low Dose ◽

Tooth Movement ◽

Orthodontic Tooth Movement ◽

Control Group ◽

High Dose ◽

Male Adult ◽

Significant Difference ◽

The Mean ◽

And Control

ObjectiveAn animal trial, its protocol approved by the Institutional Animal Care and Use Committee of the U.P. National Institutes of Health (IACUC Protocol No. 2010-008), was employed to investigate the effects of resveratrol on the degree and rate of orthodontic tooth movement in guinea pigs.Materials and MethodsEighteen male adult guinea pigs were randomly allocated into 3 groups: low dose, high dose, and control groups. A 0.016″ titanium molybdenum alloy wire formed into a helical torsion spring with a coil, with the loops cemented onto the maxillary incisors of the animals, served as the orthodontic appliance. Daily oral administration of resveratrol was provided to the low dose (0.047 mg/kg) and high dose (0.47 mg/kg) groups, while water was provided to the control group. Measurements were taken everyday at the interproximal area at the level of the incisal edge using a measuring caliper.ResultsThe results of the ANOVA showed no statistically significant differences in the mean measurements of tooth separation among the three groups from day 2 (P=0.966) to day 8 (P=0.056). However, starting from day 9 (P=0.049) until day 18 (P=0.000), there was a significant difference in the mean tooth separation among the test groups.ConclusionUsing the LSD, it was noted that the low dose and the high dose groups have similar degrees of mean tooth separation, with the control group being significantly different from the two.

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Vibratory stimulation in non-growing patients and its effect on the orthodontic tooth movement

International Journal of Scientific Reports ◽

10.18203/issn.2454-2156.intjscirep20214935 ◽

2021 ◽

Vol 8 (1) ◽

pp. 33

Author(s):

Tanushree Sharma ◽

Ankita Gupta ◽

Aseem Sharma ◽

Pulkit Vaid ◽

Kamlesh Singh

Keyword(s):

Tooth Movement ◽

Orthodontic Tooth Movement ◽

Control Group ◽

P Value ◽

Vibratory Stimulation ◽

Movement Rate ◽

Canine Retraction ◽

And Control ◽

Tooth Movement Rate ◽

The Impact

Background: Objectives of the study were to determine the impact of vibratory stimulation in non-growing patients on the orthodontic tooth movement rate and to differentiate the orthodontic tooth movement rate in both experimental and control sides.Methods: 30 non-growing patients were selected for a split-mouth study with fixed appliance therapy orthodontic treatment undergoing bilateral first premolar extractions in maxillary arch. Type 1 active tiebacks were used to perform single canine retraction in all the patients. Vibratory stimulation was provided for 15 minutes daily with the help of Oral-B battery powered toothbrushes of 125 Hz frequency. Measurement was taken with calibrated digital vernier caliper clinically and OPG were taken at regular time intervals using grid method for the tooth movement calculation.Results: P value obtained by statistical calculation shows non-significant results in both experimental and control sides in non-growing patients.Conclusions: Cyclic loading in non-growing patients with fixed orthodontic appliance shows no change in orthodontic tooth movement in experimental and control group.

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Effects of Integrated Indirect Forest Experience on Emotion, Fatigue, Stress, and Immune Function in Hemodialysis Patients

International Journal of Environmental Research and Public Health ◽

10.3390/ijerph18041701 ◽

2021 ◽

Vol 18 (4) ◽

pp. 1701

Author(s):

Hyoyoung Kang ◽

Youngran Chae

Keyword(s):

Renal Failure ◽

Chronic Renal Failure ◽

Immune Function ◽

Psychological Stress ◽

Physiological Stress ◽

Negative Emotion ◽

Hemodialysis Patients ◽

Control Group ◽

Significant Difference ◽

Stress Levels

Background: Most hemodialysis patients may experience physiological and psychological stress. Exposure to nature has been reported to reduce psychological and physiological stress levels and improve immune function. This study aimed to investigate psychological and physiological effects of integrated indirect forest experience on chronic renal failure patients undergoing hemodialysis. Methods: As a quasi-experiment, this study employed a nonequivalent control group, repeated measurements, and a non-synchronized design. In total, 54 participants were included: 26 and 28 patients in the experimental and control groups, respectively. During hemodialysis, five types of forest therapy stimuli (visual, auditory, olfactory, tactile, and motor) were applied 3 times per week for 4 weeks during 15 min sessions. Results: Positive, but not negative, emotion measures differed between the groups after the intervention. Fatigue and physiological stress levels were significantly reduced in the experimental group, whereas no significant difference was found between the groups with respect to measures of psychological stress. Activation of both the parasympathetic and sympathetic nervous systems was similar in both groups, as was the number of natural killer cells. Conclusion: Integrated indirect forest experience may help increase positive emotions and reduce fatigue and stress levels during hemodialysis in patients with chronic renal failure.

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The Effect of Low-Level Laser Therapy on the Acceleration of Orthodontic Tooth Movement

Journal of lasers in medical sciences ◽

10.34172/jlms.2020.34 ◽

2020 ◽

Vol 11 (2) ◽

pp. 204-211 ◽

Cited By ~ 1

Author(s):

Maryam Baghizadeh Fini ◽

Pooya Olyaee ◽

Ahmadreza Homayouni

Keyword(s):

Clinical Trials ◽

Laser Therapy ◽

Tooth Movement ◽

Orthodontic Tooth Movement ◽

Low Level Laser Therapy ◽

Control Group ◽

Low Level ◽

Low Level Laser ◽

Level Laser ◽

Significant Difference

In clinical practice, low-level laser therapy (LLLT) is widely used. The main aim of this review is to assess the effectiveness of LLLT in accelerating tooth movement in human subjects. The PRISMA checklist was utilized as a guideline to carry out this systematic review. The electronic databases were searched from Google Scholar (2014-2018) and PubMed, and comprehensive research on this topic was also manually conducted. Therefore, 77 articles randomized clinical trials (RCTs) or controlled clinical trials (CCTs) were selected. After screening studies, consequently, eleven trials met the inclusion criteria. Eight out of 11 studies showed LLLT has a significant impact on the acceleration of orthodontic tooth movement, and there was no statistically significant difference in the rate of tooth movement between the laser group and the control group in the two remaining studies. Furthermore, five out of 11 articles showed that LLLT has no adverse effects. Although we have some degree of understanding from a cellular point of view to LLLT effects, we still do not know whether these cellular level changes have any effect on the clinical acceleration of orthodontic tooth movement. The results are inconclusive and cannot be generalized to the public community; therefore, well-structured studies are required.

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