scholarly journals Preliminary Quantification of Phytochemicals in Methanolic Extract of Rosmarinus officinalis L Using HPTLC

Author(s):  
Pathania Singh Mamta ◽  
Singh Hitender ◽  
Guleria Puspha ◽  
Sapna Sapna ◽  
Bhardwaj Payal ◽  
...  

Rosmarinus officinalis L. commonly known as Rosemary belong to the family Lamiaceae. It is an aromatic herb which is hardy, dense and evergreen in natural habitat. It is one of the medicinal plants which is cultivated around the world for its medicinal, therapeutic purpose and is used as condiment and food preservative. It is one of the oldest Mediterranean shrub having strong, pungent aroma having dark green and elongated leaves with white or purple flowers. It is a perennial herb reaching 100 to 210 cm in height having pointed and hairy, resinous dark green leaves with lower white surface. The present study review about macroscopical/botanical description of Rosmarinus officinalis L. and its methanolic extraction analysis for preliminary quantification of chemical constituents by visualizing TLC plate under different wavelength of UV light i.e., 255nm, 366nm and 566nm to get chromatograph by HPTLC.

Author(s):  
Afroz Patan

 Objective: The main objective of review is an effort to give detailed information on Valeriana jatamansi and its multiple medicinal properties. Methods: As per the literature, the plant V. jatamansi is a perennial herb and found at subtemperate Himalayan regions of the Kumaon and Garhwal. The plant most popularly known as “Valerian.” The plant belongs to the family Valerianaceae. The roots and rhizomes of the plants are traditionally used to treat ulcers, jaundice, dry cough, seminal weakness, skin diseases, leprosy, and for sleep enhancement due to the presence of a variety of different chemical constituents such as sesquiterpenes, coumarins, iridoids, lignanoids, alkaloids, and flavonoids. From literature, the reported methods performed for different pharmacological activities have been thoroughly discussed and complied. Results: Most of the studies compiled from literature shown that V. jatamansi possess antimicrobial, antioxidant, anti-inflammatory, cytotoxic, anxiolytic, and neuroprotective activities. Conclusion: The review reveals that continuous research on this plant is needed to know the exact molecular mechanism and further elaborative studies can lead to develop safe therapeutic use in modern medicine.


HortScience ◽  
1992 ◽  
Vol 27 (6) ◽  
pp. 618a-618
Author(s):  
Azza A. Tawfik ◽  
Paul E. Read ◽  
Susan L. Cuppett

Callus of Rosmarinus officinalis L. 'Lockwood de forest' was induced from stem segments (3 mm long) using different concentrations of thidiazuron (TDZ). The original stem segments used as explants were found to have a higher level of linalool than was found for leaf segments. Linalool is one of the monoterpenes identified in rosemary plants and it has a pleasant aroma. TDZ has a significant effect on callus formation and callus texture. The callus formed was light green to yellow and/or had some meristimatic dark green cells. TDZ had a significant linear effect on the callus fresh weight. The meristimatic green cells formed on all calli except those proliferated on the lowest concentration of TDZ (0.5 mg/l). No callus was induced from stem segments cultured on TDZ-free medium. The fresh calli from other treatments were soaked in hexane as a solvent for monoterpene analysis using GC/MS. No monoterpenes could be detected in the callus induced on the medium containing the lowest concentration of TDZ. Comparing to the stem segments taken from the parent plants only 4 of 10 monoterpenes identified were found in the callus: α-pinene, β-pinene, 1,8-cineole, and camphor.


Author(s):  
SARAH ALI HAMID

Objective: The purpose of this study was to identify and characterize chemical constituents of Rosmarinus officinalis L. essential oils from Diyala Province, an eastern side of Iraq. Methods: Following hydrodistillation extraction of the leaves, gas chromatography–mass spectrometry (MS) was performed. Eighteen peaks were identified and compared to known compounds and MS patterns. Results: The two major components identified were eucalyptol (1,8-cineole) and L-camphor, and they represent 59% and 29%, respectively. In addition, α-terpineol, bornyl acetate, borneol, linalool, and β-terpineol were detected in 3.75, 2.83, 1.96, 1.22, and 0.65%, respectively. Conclusion: The present study showed that rosemary essential oils from Diyala Province of Iraq consisted mostly of oxygenated monoterpenes (>96%) and hydrocarbon monoterpenes are less than 1%. The latter suggest that hydrocarbon monoterpenes may undergo transformation due to atmospheric as well as microbiological metabolism which more studies are needed to confirm such suggestion.


2020 ◽  
Vol 10 ◽  
Author(s):  
Rizwana Khatoon ◽  
Md. Aftab Alam ◽  
Pramod Kumar Sharma

Aim: The purpose of this review paper is to elaborate many nano-formulations of Rosemary which are used against many diseases and also explain the Pharmacological activity of phytocompounds of Rosemary and details about the applications and uses of Rosemary in many fields. Objectives: Rosemary (Rosmarinus officinalis L.) is a medicinal herbal plant, their leaves are used in Europe, America, and Asia as a food additive. It is very valuable, their parts contain many chemical constituents which are very effective against many diseases like diabetes, obesity, cancer, infections and also have antioxidant, anti-inflammation, memory-improvement, etc. properties. Results: In the literature reviewed, Rosmarinus officinalis (rosemary) has shown exciting potential both as a natural food preservative and as a therapeutic agent. Rosemary (Rosmarinus officinalis) essential oil has been extensively studied due to its chemical composition and biological activities. The extract of Rosemary also shows the medicinal properties like anticancer, antimicrobial, antidiabetic, anti-inflammatory etc. From many studies, it was also found that nano-formulation of Rosemary had been developed by using the Rosemary essential oil and its extract which are used in the diagnosis and treatment of many diseases. Conclusion: From the conventional times, therapeutic effects of Rosemary are seen, which is also used in Modern Medicine now days. It had been used as food additives in the conventional times. In renal colic and dysmenorrhea, it is used as an antispasmodic to relieve respiratory disorders and to boost hair development. CNS, cardiac and locomotive activity promotes inhalation and oral administration of Rosemary oil, suggesting the direct effect of one or more of its constituents. Rosemary have curative potential in the therapy and avoidance of bronchial asthma, peptic ulcer, spasmodic disorders, inflammatory illnesses, atherosclerosis, hepatotoxicity, cancer, ischemic heart illness, bad sperm motility, Alzheimer, and may act as antidepressant and anxiolytic properties.


2017 ◽  
Vol 14 (3) ◽  
pp. 1025-1031 ◽  
Author(s):  
Tawheed Amin ◽  
H. R. Naik ◽  
Syed Zameer Hussain

ABSTRACT: The Aim of the Present Study Was to Evaluate the Yield, Chemical Constituents and Determine the Chemotype of the Essential Oil Obtained from Different Rosemary Plants Growing in Different Accessions of Rosemary Fields. About four Plant Samples Were Analyzed for Essential Oil Yield and the Essential Oil Yield Varied from 0.88% To 1.2%. the Essential Oil Samples Were Further Analyzed by Gas Chromatography (GC) for the Purpose of Identification of Chemical Constituents Present in Them. It Was Contended from the Results That the Selected Plants Differed from Each Other in Terms of Chemical Constituents.Camphor Content Was Foundin Higher Amount in All the Foursamples, Thus it Could Be Inferred That the Plants Are Camphor Chemotype.


2020 ◽  
Vol 8 (9) ◽  
pp. 4508-4515
Author(s):  
Sangeetha G. Pillai ◽  
Yadevendra Yadav ◽  
Khem Chand Sharma

Choraka is a highly valued aromatic plant endemic to the Himalayan region. Angelica glauca is a perennial herb of Umbelliferae family. It is very commonly used as a spice and for treating stomach ailments. Its es-sential oil is highly valued and has broncho-relaxant, antimicrobial, antifungal, anxiolytic and antioxidant activity. Its chemical constituents are mainly (Z)-ligustilide, (Z)-butylidenephthalide, and (E)-butyl ideneph-thalide. It has been in use since the period of Arthashastra and references can also be seen in Brihatrayi and various Nighantus for Mukha and Nasa roga chikitsa. Ruthless uprooting of the plant from its natural habitat for its sale in the market has led to a considerable decline in its availability. This article deals with its distribution, its uses in Ayurveda with classical references, researches on its extracts, controversies and the threats faced by the plant.


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