scholarly journals Effect of AZADIRACHTA INDICA (Neem) leave extract on Some Selected Bacteria

2021 ◽  
Vol 3 (Number 2) ◽  
pp. 3-6
Author(s):  
Tuhin Jahan ◽  
Zinnat Ara Begum ◽  
Sayeeda Sultana ◽  
Rokhsana Dil Afroz ◽  
Mosa Shaheli Binty Hossain
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Azadirachta Indica ◽  
Average Diameter ◽  
Salmonella Typhi ◽  
Diffusion Method ◽  
Dilution Method ◽  
Zone Of Inhibition ◽  
Agar Disc

Azadirachta indica (Neem) is an herbal plant widely distributed in our subcontinent during all seasons. This study was carried out to detect the effect of Azadirachta indica (Neem) leave extract on some selected bacteria. The prospective study was carried out during the period of July 2005 to June 2006. Micro-organisms which are commonly responsible for infection in our country such as Staphylococcus aureus, Salmonella typhi, Escherichia coli and Pseudomonas aeruginosa were considered. Extract of Neem leave that is Aqueous Neem leave extract (ANLE) was prepared by filtration and evaporation. Effect against bacteria was examined by detection of minimum inhibitory concentration (MIC) by using ‘Broth dilution method’ and by detection of bacterial susceptibility by ‘Agar disc diffusion method’ and compare with cefepime. For ANLE the MICs against Staphylococcus aureus, Salmonella typhi, Escherichia Coli and Pseudomonas aeruginosa was 800 μg/ml, 1200 μg/ml, 1400 μg/ml and 1400 μg/ml respectively. With those MICs the average diameter of zone of inhibition against staphylococcus aureus with ANLE and cefepime was 20mm and 30mm respectively. Salmonella typhi exhibit sensitivity with zone of inhibition of 19mm, 26.5mm against ANLE and cefepime. Escherichia coli were sensitive against ANLE and cefepime with 17.5mm, 23.5mm zone of inhibition respectively. For Pseudomonas aeruginosa zone of inhibition was 16mm, and 21mm by ANLE, and cefepime respectively. Aqueous Leave extract of Azadirachta indica (Neem) have antibacterial activity against ‘staphylococcus aureus, salmonella typhi, Escherichia coli and Pseudomonas aeruginosa’ compared with Cefepime. Among all test bacteria staphylococcus aureus has lowest MICs.

scholarly journals Antimicrobial Efficacy of Mineral Trioxide Aggregate against Therapy Resistant Endodontic Microorganisms

2016 ◽  
Vol 33 (3) ◽  
pp. 140-145
Author(s):  
Mst Laila Akter Banu ◽  
AKM Bashar ◽  
Md Mujibur Rahman Howlader ◽  
Md Shamsul Alam ◽  
Md Ashraf Hussain
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Bacillus Subtilis ◽  
Candida Albicans ◽  
Calcium Hydroxide ◽  
Enterococcus Faecalis ◽  
Antimicrobial Efficacy ◽  
Diffusion Method ◽  
Zone Of Inhibition

Microorganisms, usually from the dental caries, are the main sources of diseases in dental pulp (root canals) and periapical region. Facultative bacteria and fungi have been identified in therapy resistant persistent endodontic infection. The objectives of this study was to evaluate the antimicrobial efficacy of Mineral Tri Oxide Aggregate (MTA) against therapy resistant endodontic microorganisms. The efficacy of MTA was also compared with that of calcium hydroxide. Six standard bacterial stains were used: Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, bacillus subtilis, Candida albicans and Enterococcus faecalis. The agar diffusion method on Muller- Hilton media was employed. The plates containing media were inoculated with the specified bacterial suspensions. Two standard holes were prepared on each microorganism inoculated plate with a copper puncher and one hole was completely filled with MTA & the other with Ca (OH)2 . The plates were then kept at environmental temperature for one hour to ensure prediffusion and then incubated at 370C for 24 hours. After 24 hours, the diameters of inhibition zones were measured. Tests were replicated for thirty times for each sample and mean values were taken. Zone of inhibition as measured for MTA and Ca (OH)2 were statistically analyzed with Student’s t-Test and Post Hoc Games Howell Test and were presented as mean ± SD to compare of efficacy of MTA and calcium hydroxide on different microorganisms. Both MTA and Ca(OH)2 were found to produce zone of inhibition against Staphylococcus aureus (ATCC 25923), Pseudomonas aeruginosa (ATCC 27853), , Bacillus subtilis (BTCC 17 ), and Candida albicans (BTCC 493). MTA showed highest activity against S. aureus and lowest activity against P. aeruginosa which was similar to the activity range of Ca (OH)2 against the mentioned organisms. But both of them failed to produce any activity against E. coli and. E. faecalis. MTA was found to produce a lower efficacy than Ca (OH)2 while comparing the zone of inhibition between them and statistically it was significant. Mineral Tri Oxide Aggregate (MTA) showed antimicrobial efficacy against some therapy resistant microorganisms but it did not show antimicrobial efficacy against Escherichia coli and Enterococcus faecalis. MTA was found to produce a lower antimicrobial efficacy than Ca (OH)2.J Bangladesh Coll Phys Surg 2015; 33(3): 140-145

scholarly journals SYNTHESIS AND ANTIMICROBIAL PROFILE OF SOME NEWER HETEROCYCLES BEARING THIAZOLE MOIETY

2012 ◽  
Vol 20 (20) ◽  
pp. 61-67
Author(s):  
RAJUL GUPTA ◽  
Neeraj Kumar FULORIA ◽  
Shivkanya FULORIA
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Candida Albicans ◽  
Acetic Anhydride ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
Zone Of Inhibition ◽  
Disk Diffusion Method ◽  
Antibacterial And Antifungal

Various substituted acetophenones on treatment. with iodine and thiourea yielded 2-amino-4-( substituted-phenyl)-thiazole, which on further treatment with acetic anhydride generated N-(4-(substituted phenyl)thiazol-2-ylacetamide(1-5). All the synthesized compounds were characterized by their respective FTIR, 1H NMR, and mass data. Synthesized compounds (l, 2, 3, 4, 5) when subjected to investigation for their antimicrobial activities i.e. antibacterial and antifungal studies against Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Candida albicans, Aspergillus flavus, and Aspergillus fumigatus by disk diffusion method, revealed that compound 2 deemed to be most potent with the largest zone of inhibition.

scholarly journals Synthesis and Evaluation of Some New Thiazolidin-4-One Derivatives as Potential Antimicrobial Agents

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
B. A. Baviskar ◽  
S. S. Khadabadi ◽  
S. L. Deore
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Antimicrobial Agents ◽  
Salmonella Typhi ◽  
Zone Of Inhibition ◽  
Structure Activity

A new series ofN-{4-methyl-5-[4-(4-oxo-2-phenyl(1,3-thiazolidin-3-yl)]-5-sulfanyl(1,2,4-triazol-3-yl)-1,3-thiazol-2-yl }acetamide (7a-l) was synthesized in order to determine their antimicrobial activity and feasible structure–activity relationships. The compounds were synthesized in good yield and the structures of all newly synthesized compounds were established on the basis of their IR,1HNMR, and elemental analysis. The synthesized compounds were testedin vitroantibacterial activity againstStaphylococcus aureus,Escherichia coli,Pseudomonas aeruginosaandSalmonella typhiand antifungal activity againstAspergillus niger,Candida albicansby measuring the zone of inhibition in mm.

scholarly journals Antimicrobial Activity and Phytochemical Screening of Euphorbia helioscopia

2020 ◽  
Vol 38 ◽  
Author(s):  
K. WAHEED ◽  
S.K. MUHAMMAD ◽  
A. SHOMAILA ◽  
Z. MUHAMMAD ◽  
U. IZHAR ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antimicrobial Activity ◽  
Bacillus Subtilis ◽  
Water Extract ◽  
Salmonella Typhi ◽  
Fungal Strain ◽  
Phytochemical Screening ◽  
Zone Of Inhibition

ABSTRACT: Medicinal Plants have been used throughout the world by human beings as a drug and remedies for various diseases since time immemorial. A study was planned to count into the antimicrobial activity and phytochemical screening of Euphorbia helioscopia. The plants were gathered and tested against some standard strains and some human pathogenic microorganisms i.e Escherichia coli, Bacillus Subtilis, Staphylococcus aureus, Klebsiella pneumoniae, Salmonella typhi, Pseudomonas aeruginosa and three fungal strain Trichoderma, R hizopus nigricans, Aspergillus niger. The concentrations of extracting samples (500 and 1,000 mg mL-1) were used against pathogens. Ciprofloxacin was used as positive control in case of bacterial strains and Colfrimazol was used against the fungal strain while dimethyl sulfoxide as negative control. The outcomes indicated that the positive wells potency of Water extract had a 36 mm diameter of zone of inhibition against Escherichia coli and ethanol extract at 1,000 mg mL-1 had maximum (34 mm) zone of inhibition against Bacillus subtilus (36 mm) zone of inhibition against Klebsiella pneumonia and 33 mm of zone of inhibition against Trichoderma harzianum. Likewise, water extract at a concentration of 1,000 mg mL-1 resulted highest value of zone of inhibition (36 mm) against Staphylococcus aureus, a zone of inhibition ( mm) against Salmonella typhi, 36 mm zone of inhibition against Pseudomonas aeruginosa, (32 mm) zone of inhibition against Rhizopus nigricans, a 34 mm zone of inhibition against Acremonium and (34 mm) zone of inhibition against Aspergillus niger. The most susceptible bacteria were K. pneumonia and Bacillus subtilis, while E. coli was the most resistant bacteria and showed zone of inhibition. The ethanolic extract had tannins, lipid, total proteins, carbohydrates, flavonoids, Alkaloid and polyphenolics.

scholarly journals MOMORDICA CHARANTIA L. (bitter gourd) AS A CANDIDATE FOR THE CONTROL OF BACTERIAL AND FUNGAL GROWTH

2019 ◽  
Vol 56 (04) ◽  
pp. 1031-1036
Author(s):  
M. Shahid Mahmood
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Cost Effective ◽  
Salmonella Typhi ◽  
Bitter Gourd ◽  
Penicillium Expansum ◽  
Diffusion Method ◽  
Zones Of Inhibition ◽  
Agar Well Diffusion Method

The plants use for medicinal purposes have been experienced by an extensive proportion of population of population for many centuries. The present study was designed to formulate cost effective dietary interventions to prevent and treat certain microbial diseases. The current experiment was conducted for evaluation of antifungal and antibacterial effects of bitter gourd (Momordica charantia L.), which is locally named as Karella. All parts of bitter gourd were used including seeds, pulp and skin. Its methanolic extracts were taken to check its antibacterial efficacy against Staphylococcus aureus, Salmonella typhi, Pseudomonas aeruginosa, Escherichia coli and methicillin resistance Staphylococcus aureus (MRSA) by paper disc diffusion test. Antifungal efficacy against selected species of Penicillium and Aspergillus was done by Agar Well-Diffusion Method. Broth Dilution method and Agar Well-Diffusion Method were used to determine the minimum inhibitory concentration (MIC). Zones of inhibition diameters were 11mm, 7mm, 11mm and 9mm for Staphylococcus aureus, Salmonella typhi, Pseudomonas aeruginosa and Escherichia coli, respectively. There was no zone of inhibition against MRSA. The zones of inhibition for bitter gourd seed were 12mm, 8mm, 12mm and 11mm for Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa and Salmonella typhi, respectively. The MIC of bitter gourd pulp against Aspergillus niger and Penicillium expansum was 400mg/ml and 600mg/ml, respectively. The MIC of bitter gourd seed against Aspergillus niger and Penicillium expansum was 300mg/ml and 400mg/ml, respectively. Taking together, bitter ground was found a cost effective antibacterial and antifungal agent, it may be used an effective alternate remedial option

scholarly journals Preparation and Antibacterial Properties of Substituted 1,2,4-Triazoles

2015 ◽  
Vol 2015 ◽  
pp. 1-5 ◽  
Author(s):  
Ionuţ Ledeţi ◽  
Vasile Bercean ◽  
Anda Alexa ◽  
Codruţa Şoica ◽  
Lenuţa-Maria Şuta ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Biological Activities ◽  
Antibacterial Properties ◽  
Pharmaceutical Formulations ◽  
Diffusion Method ◽  
Dilution Method ◽  
Bacterial Strains ◽  
Disk Diffusion Method

Background. Both 1,2,3- and 1,2,4-triazoles are nowadays incorporated in numerous antibacterial pharmaceutical formulations.Aim. Our study aimed to prepare three substituted 1,2,4-triazoles and to evaluate their antibacterial properties.Materials and Methods. One disubstituted and two trisubstituted 1,2,4-triazoles were prepared and characterised by physical and spectroscopic properties (melting point, FTIR, NMR, and GC-MS). The antibacterial properties were studied against three bacterial strains:Staphylococcus aureus(ATCC 25923),Escherichia coli(ATCC 25922), andPseudomonas aeruginosa(ATCC 27853), by the agar disk diffusion method and the dilution method with MIC (minimal inhibitory concentration) determination.Results. The spectroscopic characterization of compounds and the working protocol for the synthesis of the triazolic derivatives are described. The compounds were obtained with 15–43% yields and with high purities, confirmed by the NMR analysis. The evaluation of biological activities showed that the compounds act as antibacterial agents againstStaphylococcus aureus(ATCC 25923), while being inactive againstEscherichia coli(ATCC 25922) andPseudomonas aeruginosa(ATCC 27853).Conclusions. Our results indicate that compounds containing 1,2,4-triazolic moiety have great potential in developing a wide variety of new antibacterial formulations.

scholarly journals Antibacterial Property of Extract of Erveniastrum nepalense (Edible Lichen) Collected from Hilly Regions of Eastern Nepal

2019 ◽  
Vol 6 ◽  
pp. 51-58
Author(s):  
Saraswati Yonghang ◽  
Suman Rai ◽  
Shiv Nandan Sah
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Bacillus Subtilis ◽  
Minimum Inhibitory Concentration ◽  
Inhibitory Concentration ◽  
Antibacterial Property ◽  
Diffusion Method ◽  
Zone Of Inhibition ◽  
Klebsiella Spp

Objectives: To evaluate the antibacterial property of Everniastrum nepalense(edible lichen)collected from eastern part of Nepal and determine Minimum Inhibitory Concentration (MIC) of lichen extract. Methods: Everniastrum nepalense was collected from local Rai and Limbu community in a plastic bag from four hilly districts (Pachthar, Taplejung, Dhankuta and Bhojpur) of Eastern, NepaMoisture content in lichen samples were removed by air drying and grinded to powdery form. The methanolic extracts of lichens of different places were prepared by solvent extraction process using Soxhlet apparatus and tested against human pathogenic bacteria by disc diffusion method. Then, Minimum Inhibitory Concentration (MIC) of lichen extracts was determined using well diffusion method. Results: The lichens extract against the test bacterial isolates were performed by agar well diffusion method. Overall, it was observed that all these lichen extracts from Eastern Nepal had inhibitory effect on both gram-positive and gram-negative bacteria. Out of seven bacterial samples used, all bacterial samples were inhibited by the lichen extracts except Escherichia coli. The effect of lichen extract collected from Bhojpur district had maximum zone of inhibition against Staphylococcus aureus, Salmonella Typhi and Klebsiella spp were found to be 13 mm, 14 mm and 14 mm respectively. Similarly, zone of inhibition against Bacillus subtilis was found to be 17 mm from samples of Dhankuta, which was highest value than other 3 districts viz; Bhojpur, Taplejung and Pachthar District. Likewise, Pseudomonas aeruginosa showed highest inhibition value i.e. 18 mm on Pachthar sample whereas, Shigella showed 15 mm on Dhankuta sample. This study revealed that inhibitory capacity of edible lichen (Everniastrum nepalense) against test bacteria was not the same. It varied from place to place. Conclusion: This study has concluded that Everniastrum nepalensehas potential antibacterial property against Bacillus subtilis, Staphylococcus aureus, Escherichia coli, Salmonella spp, Shigella spp, Klebsiella spp and Pseudomonas aeruginosa except E. coli along with nutritional value and can be used as safe alternative and economic herbal medicine to treat infectious diseases.

scholarly journals ISOLASI DAN UJI ANTIBAKTERI DARI ISOLAT BAKTERI YANG BERASOSIASI DENGAN SPONS Callyspongia aerizusa SERTA IDENTIFIKASI SECARA BIOKIMIA

PHARMACON ◽  
2019 ◽  
Vol 8 (2) ◽  
pp. 380
Author(s):  
Angelika Liempepas ◽  
Widya A. Lolo ◽  
Paulina V. Y. Yamlean
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Streptococcus Pyogenes ◽  
Staphylococcus Epidermidis ◽  
Health Sector ◽  
Salmonella Typhi ◽  
Diffusion Method ◽  
Agar Disc ◽  
And Staphylococcus Aureus

ABSTRACT               Sponge Callyspongia aerizusa contain potential bioactive compound that can be utilized in the health sector. Extract of sea sponge Callyspongia aerizusa, can hamper the growth of Salmonella typhi bacteria, Streptococcus pyogenes, Shigella and Staphylococcus epidermidis. The aim of this study was to test the antibacterial activity against Escherichia coli and Staphylococcus aureus bacteria and identify the type of symbionic bacteria of Callyspongia aerizusa sponge based on their physiological and biochemical characteristics. The method of testing the antibacterial activity was agar diffusion method (Kirby and Baurer diffusion disc). There were three bacterial isolates namely T1, T2, and T3 isolates. The result showed that T1, T2, and T3bacterial isolates had antibacterial activity against Escherichia coli and Staphylococcus aureus test bacteria. Based on the biochemical test, T2bacterial isolates were identified as Bronchothrix bacteria and T1and T3 bacterial identified as Desulfotomaculum. Keywords: Callyspongia aerizusa, Antibacterial activity, symbiont bacteria, Biochemical Identification   ABSTRAKSpons Callyspongia aerizusa memiliki kandungan senyawa bioaktif potensial yang dapat dimanfaatkan dibidang kesehatan. Ekstrak spons laut Callyspongia aerizusa dapat menghambat pertumbuhan bakteri Salmonella typhi, Streptococcus pyogenes, Shigella dan Staphylococcus epidermidis. Penelitian ini bertujuan untuk menguji aktivitas antibakteri dari bakteri simbion spons Callyspongia aerizusa terhadap bakteri Escherichia coli dan Staphylococcuc aureus dan mengidentifikasi jenis bakteri simbion spons Callyspongia aerizusa berdasarkan karakteristik fisiologis dan biokimianya. Metode pengujian aktivitas antibakteri yang digunakan yaitu metode difusi agar (disc diffusion Kirby and Baurer). Terdapat tiga isolat bakteri yaitu isolat T1, T2, dan T3. Hasil penelitian menunjukan bahwa isolat bakteri T1, T2, dan T3 memiliki aktivitas antibakteri terhadap bakteri uji Escherichia coli dan Staphylococcuc aureus. Berdasarkan uji biokima, isolat bakteri T2 diduga sebagai bakteri Brochothrix dan  isolat bakteri T1 dan T3 diduga sebagai bakteri Desulfotomaculum.Kata kunci: Callyspongia aerizusa, Aktivitas antibakteri, Bakteri simbion, Identifikasi Biokimia

scholarly journals Phytochemical Screening and Antibacteria/ Antifungi Activities of Root and Shoot Extracts of Euphorbia hirta (Asthma Weed)

2020 ◽  
pp. 1-10
Author(s):  
L. A. Adjeroh ◽  
M. O. Nwachukwu ◽  
P. N. Abara ◽  
J. C. Nnokwe ◽  
J. N. Azorji ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Streptococcus Pyogenes ◽  
Aqueous Extract ◽  
Salmonella Typhi ◽  
Diffusion Method ◽  
Root Extract ◽  
Phytochemical Screening ◽  
Zone Of Inhibition ◽  
Euphorbia Hirta

Medicinal plants, also called herbal medicine, have been used in traditional medicine practices since prehistoric times. The phytochemical screening of root and shoot extracts of Euphorbia hirta plant commonly known as asthma weed was evaluated using soxhlet and aqueous extract as a solvent to determine the active components. Maceration method was used in extracting the active properties/component. Phytochemical screening of root and shoot extracts revealed presences of saponins, anthranoid anthroqunione, phenol, alkaloid, tannins, phylobatannins and cardiac glycoside. Antibacterial screening of clinical isolates of Staphylococcus aureus, Salmonella typhi, Escherichia coli and Streptococcus pyogenes, using disk diffusion method, showed that in both the aqueous root and shoot extract Streptococcus pyogenes has the highest zone of inhibition of 120 mg with 12mm while least is Escherichia coli that had no inhibition at all. The aqueous extract the root and shoot were more active than the soxhlet solution. Using the aqueous shoot extracts, Streptococcus at 120 mg with 12mm zone of inhibition of Staphylococcus at 90 mg with 9mm. While in the aqueous root extract, Staphylococcus aureus at 100 mg with 10mm, Streptococcus pyogenes at 90 mg with 9m and Salmonella typhi at 80mm with 8mm. Antifungal screening with clinical isolate of candida albicans had highest zone of inhibition 130 mg with 13mm at root aqueous extract while penicillium spp, Aspergillus, spp and Microsporium spp showed no zones of inhibition at both root and shoot extracts. The results obtained suggested that Euphorbia hirta plant can be used in the treatment of ailments caused by the test microorganisms, with particular attention being paid to its aqueous extract.

scholarly journals Evaluation of Bactericidal Activity of Garcinia Gummi-Gutta and Gymnema Sylvestre- A Plant of Ethano Medicinal Importance

2019 ◽  
Vol 8 (2S11) ◽  
pp. 722-725
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Wide Spectrum ◽  
Salmonella Typhi ◽  
Gymnema Sylvestre ◽  
Zone Of Inhibition ◽  
Gram Positive ◽  
Gram Negative ◽  
Dried Fruit

Antibiotic defiance in bacterial is a satisfactory obstacle in the latest world. Day-to-day novel dopes are being intact to defeat this drawback. The goal of this is trained used to be to check antimicrobial resistance of the Garcinia gummi-gutta and Gymnema Sylvestre. The dried fruit of G. Gummi-gutta and G.Sylvestre crops are located in a southern state of India. The extract of dried fruit of G. Gummi-gutta and G.Sylvestre leaf was once ready through using specific solvent of Ethyl Acetate. These ready extracts have been used for evaluation of antibacterial ability in opposition to three Gram-positive bacterial stains (Escherichia coli, Salmonella typhi, Pseudomonas aeruginosa) and two gram-negative bacterial stains (Streptococcus, Staphylococcus aureus). The antimicrobial ability was appraised by Disc diffusion assay of G. Gummi-gutta and G.Sylvestre. The zone of inhibition used to be determined at the concentration of (500µg/ml) in G.Gummi-gutta Streptococcus& Pseudomonas aeruginosa (14mm), Staphylococcus aureus& Salmonella typhi (9mm), Escherichia coli (8mm). The zone of inhibition was once determined at the concentration of (500µg/ml) in G.Sylvestre.The Streptococcus(11mm), Staphylococcus aureus& Escherichia coli (9mm), Salmonella typhi(10mm), Pseudomonas aeruginosa (8mm) respectively. Due to the fact, the extract confirmed a wide spectrum of the antimicrobial undertaking of each Gram positive and gram negative bacterial isolates. Comparative reports of the G. Gummi-gutta and G.Sylvestre.The nice one among G.Sylvestre. Can be utilized as a bio preservative or therapeutic agent to prevent a few illnesses.The dried leaves of G.Sylvestre would characterize a novel antimicrobial obtain with steady, adoptive energetic compounds that may prove a scientific base for the consumer in recent treatment.

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