An In vitro Evaluation of the Antimicrobial Activity of Nine Root Canal Sealers

2008 ◽  
Vol 9 (5) ◽  
pp. 60-67
Author(s):  
Leena Smadi ◽  
Azmi Mahafzah ◽  
Ameen Khraisat
Keyword(s):  
Antimicrobial Activity ◽  
Root Canal ◽  
Bacterial Strains ◽  
A Value ◽  
Agar Diffusion Test ◽  
Ah Plus ◽  
Microbial Strains ◽  
And Control ◽  
Root Canal Sealers

Abstract Aim The purpose of this in vitro study was to analyze the antimicrobial activity of root canal sealers by using the agar diffusion test (ADT). Methods and Materials Three categories of root canal sealers were included in the study: resin-based sealers (4), zinc oxide-based sealers (3), and calcium hydroxide-based sealers (2). The microbial strains used were: S. aureus (2 strains), C. albicans (2 strains), and E. faecalis (1 strain). Statistical analysis was conducted using a one-way analysis of variance (ANOVA). Tests of differences were analyzed using the Tukey's test with a value of p < 0.05 considered statistically significant. Results The antimicrobial activity of root canal sealers was ranked in descending order as follows: Sealite Regular, Cortisemol, Dentalis KEZ, AH26, Sealapex, Acroseal/Topseal, and Endorez/AH plus. Conclusion Root canal sealers showed different inhibitory effects depending on their types and the bacterial strains tested. Root canal sealers containing formaldehyde and eugenol proved to be effective against the microorganisms studied. Clinical Significance The incorporation of antimicrobial components into root canal sealers may become an essential factor in preventing the re-growth of residual bacteria and control of bacteria re-entry into the root canal system. Citation Smadi L, Mahafzah A, Khraisat A. An In vitro Evaluation of the Antimicrobial Activity of Nine Root Canal Sealers. J Contemp Dent Pract 2008 July; (9)5:060-067.

An Analysis of the Antimicrobial Activity of Ten Root Canal Sealers - A Duration Based in vitro Evaluation

2008 ◽  
Vol 33 (2) ◽  
pp. 117-122 ◽  
Author(s):  
Prasanna Neelakantan ◽  
Subbarao CV
Keyword(s):  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Enterococcus Faecalis ◽  
Root Canal ◽  
Antimicrobial Action ◽  
Agar Diffusion Test ◽  
Significant Difference ◽  
Ah Plus ◽  
Root Canal Sealers

Aim and Design: Sealers with prolonged antimicrobial activity are needed by the fact that microorganisms are commonly recovered from teeth with failed endodontic therapy. The aim of this study was to evaluate the duration of antimicrobial activity of ten root canal sealers (Apexit plus, Tubli Seal Xpress, Endoflas FS,Endomethasone, Endomethasone N, AH Plus, Epiphany, EndoRez, Ketac Endo, Roeko Seal) against Enterococcus faecalis and Candida albicans by the agar diffusion test. The zones of inhibition were examined immediately and after 24, 48, 72 hours, 5 and 7 days. Statistical analysis was done using Kruskal - Wallis test and Mann Whitney U test. Results and conclusions: Against Enterococcus faecalis, Endoflas FS showed the largest inhibitory zones immediately and 24 hours after manipulation, whereas, there was no significant difference between Endoflas FS and Endomethasone after 48 hours. Against Candida albicans, Endoflas FS performed better than the other sealers. All the sealers (except AH Plus, Epiphany and Roeko Seal) demonstrated higher antimicrobial action in the first 24 hours after manipulation The antimicrobial action of all the sealers (except AH Plus and Roeko Seal which showed no antimicrobial activity in any studied time and Epiphany which ceased to show any antimicrobial action after 24 hours) decreased significantly with time.

scholarly journals In vitro evaluation of the antimicrobial activity of five root canal sealers

2004 ◽  
Vol 15 (1) ◽  
pp. 30-35 ◽  
Author(s):  
Brenda Paula Figueiredo de Almeida Gomes ◽  
José Assis Pedroso ◽  
Rogério Castilho Jacinto ◽  
Morgana Eli Vianna ◽  
Caio Cezar Randi Ferraz ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antimicrobial Properties ◽  
Contact Method ◽  
Streptococcus Sanguis ◽  
Actinomyces Naeslundii ◽  
Agar Diffusion Test ◽  
Significant Difference ◽  
Ah Plus ◽  
Endodontic Sealers

The aim of the present study was to analyze the antimicrobial properties of five endodontic sealers: Endo Fill, Endomethasone, Endomethasone N, Sealer 26 and AH-Plus, against the following microorganisms: Candida albicans, Staphylococcus aureus, Enterococcus faecalis, Streptococcus sanguis and Actinomyces naeslundii. The sealers were tested immediately, 24 h, 48 h and 7 days after manipulation.The direct contact method through the observation of the microbial growth in liquid medium and the agar diffusion test were used to evaluate the antimicrobial properties of the sealers. The results, in both methodologies used, showed that immediately after manipulation, Endo-Fill and Endomethasone demonstrated the highest antimicrobial activity, with no statistically significant difference between them. Sealer 26 demonstrated the lowest antimicrobial activity. At all other times after manipulation, there were no statistically significant differences among all the sealers tested. In conclusion, none of the sealers totally inhibited the growth of the microorganisms. Furthermore, the antimicrobial activity of each sealer decreased with time and was dependent upon the microbial susceptibility to them.

scholarly journals Biocompatibility and Mineralization Activity of Three Calcium Silicate-Based Root Canal Sealers Compared to Conventional Resin-Based Sealer in Human Dental Pulp Stem Cells

Materials ◽  
2019 ◽  
Vol 12 (15) ◽  
pp. 2482 ◽  
Author(s):  
Deog-Gyu Seo ◽  
Donghee Lee ◽  
Yong-Min Kim ◽  
Dani Song ◽  
Sin-Young Kim
Keyword(s):  
Cell Migration ◽  
Root Canal ◽  
Calcium Silicate ◽  
Dental Pulp ◽  
Experimental Period ◽  
Control Groups ◽  
Human Dental Pulp ◽  
Ah Plus ◽  
And Control ◽  
Root Canal Sealers

The purpose of this study was to compare the cytotoxic effects and mineralization activity of three calcium silicate-based root canal sealers to those of a conventional resin-based sealer. Experiments were performed using human dental pulp stem cells grown in a monolayer culture. The root canal sealers tested in this study were EndoSequence BC Sealer (Brasseler), BioRoot RCS (Septodont), Endoseal MTA (Maruchi), and AH Plus (Dentsply DeTrey). Experimental disks 6 mm in diameter and 3 mm in height were made and stored in a 100% humidity chamber at 37 °C for 72 h to achieve setting. The cytotoxicity of various root canal sealers was evaluated using a methyl-thiazoldiphenyl-tetrazolium (MTT) assay. To evaluate cell migration ability, a scratch wound healing method was used, and images of the scratch area were taken using a phase-contrast microscope. Cell morphology was evaluated by a scanning electron microscope after direct exposure for 72 h to each sealer disk. In the cell viability assay, there were no significant differences between the EndoSequence BC, BioRoot RCS, Endoseal MTA, and control groups in any experimental period (p > 0.05). In the cell migration assay, there were no significant differences between the EndoSequence BC, Endoseal MTA, and control groups in any experimental period (p > 0.05). BioRoot RCS exhibited slower cell migration relative to EndoSequence BC and Endoseal MTA for up to 72 h (p < 0.05). Conversely, it showed a similar wound healing percentage at 96 h (p > 0.05). In an evaluation of cell morphology, cells in direct contact with EndoSequence BC, BioRoot RCS, and Endoseal MTA disks showed superior spreading compared to those in contact with the AH Plus disk. In an Alizarin red staining assay, EndoSequence BC, BioRoot RCS, and Endoseal MTA showed a significant increase in mineralized nodule formation compared to the AH Plus group (p < 0.05). In conclusion, all calcium silicate-based root canal sealers tested in this study showed good biological properties and mineralization activity compared to conventional resin-based sealer.

scholarly journals Constituents and antimicrobial activity of the essential oils of six Himalayan Nepeta species

2010 ◽  
Vol 75 (6) ◽  
pp. 739-747 ◽  
Author(s):  
Dinesh Bisht ◽  
Rajendra Padalia ◽  
Lalit Singh ◽  
Veena Pande ◽  
Priyanka Lal ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oil ◽  
Essential Oils ◽  
Antagonistic Activity ◽  
Significant Activity ◽  
Variable Degree ◽  
Bacterial Strains ◽  
Fungal Strains ◽  
Microbial Strains

The essential oils from six Himalayan Nepeta species, viz. Nepeta leucophylla Benth., Nepeta discolor Royle ex Benth., Nepeta govaniana Benth., Nepeta clarkei Hook f., Nepeta elliptica Royle ex Benth. and Nepeta erecta Benth., were tested for their in vitro antimicrobial activity against six pathogenic bacterial and two fungal strains. The results showed that Pseudomonas aeruginosa was the most sensitive strain tested to the essential oils of Nepeta species. The essential oils of N. elliptica and N. erecta exhibited the highest activity against P. aeruginosa, followed by the essential oils of N. leucophylla and N. clarkei. The essential oils from N. elliptica and N. erecta were also found to be very effective against Serratia marcescens; while the essential oil from N. leucophylla displayed significant activity against Proteus vulgaris and Staphylococcus aureus. Other bacterial strains displayed variable degree of susceptibility against one or more of the tested essential oils. The essential oil from N. leucophylla also showed the highest antifungal activity against both tested fungal strains, viz. Candida albicans and Trichophyton rubrum, followed by the essential oils from N. clarkei, N. govaniana and N. erecta. Iridodial derivatives, viz. iridodial ?-monoenol acetate (25.4 %), dihydroiridodial diacetate (18.2 %) and iridodial dienol diacetate (7.8 %) were identified as the major constituents of N. leucophylla, while the essential oils from N. elliptica and N. erecta were dominated by (7R)-trans, trans nepetalactone (83.4 %) and isoiridomyrmecin (66.7 %), respectively. The essential oil of N. discolor was characterized by 1,8-cineole (25.5 %) and ?-caryophyllene (18.6 %), while N. clarkei was dominated by ?-sesquiphellandrene (22.0 %) and germacrene D (13.0 %). Isoiridomyrmecin (35.2 %) and pregeijerene (20.7 %) were identified as the major constituents of N. govaniana. In general the Nepeta species containing constituents with an iridoid or lactone skeleton were found to have the greater antagonistic activity against most of the microbial strains as compared to those containing regular terpene constituents.

scholarly journals Właściwości alkalizujące wybranych krzemianowo-wapniowych uszczelniaczy kanałowych. Badanie in vitro

2018 ◽  
Vol 64 (4) ◽  
Author(s):  
Przemysław Reszka ◽  
Łukasz Kucharski ◽  
Adam Klimowicz ◽  
Mariusz Lipski
Keyword(s):  
Root Canal ◽  
Calcium Silicate ◽  
In Vitro Study ◽  
Root Canal Sealer ◽  
Ph Values ◽  
Water Ph ◽  
Ah Plus ◽  
Ph Level ◽  
Root Canal Sealers

ABSTRACTIntroduction: The aim of this in vitro study was to assess the pH values of selected calcium-silicate root canal sealers in comparison with AH Plus resin-based root canal sealer.Materials and methods: Six root canal sealers were included in this study: BioRoot RCS (Septodont, France), GuttaFlow bioseal (Coltėne/Whaledent, Switzerland), MTA Fillapex (Angelus, Brazil), Total Fill BC Sealer (FKG, Switzerland), Well-Root ST (Vericom, Korea) and AH Plus (Dentsply, DeTrey, Germany). Materials samples (n = 6) were placed in dishes and immersed in 10 mL of distilled water (pH = 6.8). After 1, 3, 24, 48, 72, 168, 336 and 504 hrs, water pH was determined with a pH meter calibrated with a solution of known pH. Data were statistically analyzed using Student-t test (p < 0.05).Results: There were differences in the water pH between the materials tested. The highest water pHs were exhibited by Total Fill BC Sealer, Well-Root ST and BioRoot RCS (pH ~12), followed by GuttaFlow bioseal (pH ~10.5) and MTA Fillapex (pH ~9). The lowest pH was shown by AH Plus (pH ~8).Conclusions: All calcium-silicate root canal sealers studied are able to elevate the pH level, which could have biological and antimicrobial effects. The AH Plus root canal sealer has poor alkalizing properties.

scholarly journals In vitro antimicrobial activity of root canal sealers and calcium hydroxide paste

2010 ◽  
Vol 1 (3) ◽  
pp. 164 ◽  
Author(s):  
AlessandroL Cavalcanti ◽  
FranciscoI.R Limeira ◽  
EvelineA.L.S Sales ◽  
AnaA.G Oliveira ◽  
DenedM.B Lima ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Calcium Hydroxide ◽  
Root Canal ◽  
In Vitro Antimicrobial Activity ◽  
Root Canal Sealers

scholarly journals Antimicrobial Activity of Two Root Canal Filling Pastes in Deciduous Teeth: an in Vitro Study

2015 ◽  
Vol 17 (1) ◽  
pp. 5
Author(s):  
Andréia Bolzan de Paula ◽  
Janaina De Cássia Orlandi Sardi ◽  
Mariana Minatel ◽  
Regina Maria Puppin-Rontani
Keyword(s):  
Antimicrobial Activity ◽  
Root Canal ◽  
Antimicrobial Property ◽  
Brain Heart Infusion ◽  
In Vitro Study ◽  
Biological Properties ◽  
Deciduous Teeth ◽  
Root Canal Filling ◽  
Agar Diffusion Test

<p>Endodontic treatment in deciduous teeth is very important so that we can preserve the primary dentition and thus promote appropriate craniofacial development. However, the use of filling pastes that have some important biological properties for the preservation of the tooth is required. The objective this study was to evaluate the in vitro antimicrobial activity of two root canal filling pastes in deciduous teeth, Vitapex® and Calcipex®, on microbial species commonly found in endodontic infections (Candida albicans, Enterococcus faecalis, Streptococcus mutans, Streptococcus sanguinis, Escherichia coli and Staphylococcus aureus), using the agar diffusion test. The experiment was carried out on BHI (Brain Heart Infusion) plates in four equidistant points, which were immediately filled with the sealers Vitapex® and Calcipex®. The<br />chlorhexidine gluconate 1% (CHX) and distilled water were used as positive and negative controls, respectively. After incubation of plates at 37 °C for 24 h, the diameter of the growth inhibition zones around the wells was measured (in millimeters) with a digital caliper with reflected light. The test was performed in triplicate and data were submitted to ANOVA and Tukey’s test (α = 0.05). Both pastes had antimicrobial activity, but Calcipex® was more efficient for most strains. The halo formed in the experiments with S. mutans and S. sanguinis was similar for both pastes. This study suggests that Calcipex® had the highest antimicrobial property with respect to the microorganisms studied.</p>

scholarly journals Influence of ultrasonics on the penetration depth of AH plus, acroseal, and EndoREZ root canal sealers: An in vitro study

2018 ◽  
Vol 21 (2) ◽  
pp. 221
Author(s):  
Abhishek Sankhala ◽  
PKrishna Prasad ◽  
Aastha Tiwari ◽  
Shrikant Parakh ◽  
GaganR Madan ◽  
...  
Keyword(s):  
Penetration Depth ◽  
Root Canal ◽  
In Vitro Study ◽  
Vitro Study ◽  
Ah Plus ◽  
Root Canal Sealers
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