Oxidative Status of Saliva and Plasma in Diabetic Children

ABSTRACT Aim The objective of this study was to determine the oxidative status of saliva and plasma in diabetic children, by analyzing advanced oxidation protein products (AOPPs) and total antioxidant capacity (TAC). Materials and methods Study included 60 patients with diabetes mellitus type I (DMT1) aged 12.45 ± 2.65 years, and 40 healthy age-matched controls. The AOPP and TAC of the plasma and saliva samples were determined using a commercial QuantiChrom™ Antioxidant Assay Kit (DTAC-100) for TAC determination, and Immunodiagnostic AG [enzyme-linked immunosorbent assay kit for AOPP]. Results Values of salivary and plasma AOPP were lower in diabetic patients than in healthy controls, while value of TAC was clinically and significantly higher in plasma of controls, and clinically higher in saliva of healthy control group, compared with diabetic patients. Average value of hemoglobin A1c (HbA1c) was 7.58 ± 0.85%. Conclusion Results of this study showed that diabetes mellitus as a condition, with well-controlled HbA1c, has no influence on AOPP levels in saliva and plasma, while TAC levels of saliva and plasma are lower in diabetic patients, which means that DMT1 has an influence on the TAC. How to cite this article Porovic S, Juric H, Dinarevic SM. Oxidative Status of Saliva and Plasma in Diabetic Children. Donald School J Ultrasound Obstet Gynecol 2017;11(2):169-173.

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Clinical efficacy of oxidative stress correction by actovegin in diabetic polyneuropathy at type 2 diabetic patients with arterial hypertension

Diabetes Mellitus ◽

10.14341/2072-0351-5680 ◽

2010 ◽

Vol 13 (2) ◽

pp. 84-89

Author(s):

Ivan Petrovich Gorshkov ◽

Vladimir Ivanovich Zoloedov ◽

Anna Petrovna Volynkina

Keyword(s):

Oxidative Stress ◽

Arterial Hypertension ◽

Diabetic Polyneuropathy ◽

Oxidative Capacity ◽

Control Group ◽

Diabetic Patients ◽

Type 2 Diabetic Patients ◽

Patients With Diabetes ◽

Total Antioxidant

Aim. To study Actovegin efficacy in oxidative stress (OS) correction at diabetic polyneuropathy (DPN) in patients with diabetes mellitus type 2 (DM2)and arterial hypertension (AH).Materials and Methods. 51 patients (24 women and 27 men) aged 53.4?0.7 with the average duration of DM2 5.6?0.2 years, DPN - 4.9?0.2years and AH - 6.0?0.2 years were examined. Daily albuminuria, glomerular filtration rate (GRF) were evaluated, standard methods for diagnosisof DPN were used. 26 patients took Actovegin therapy during 6-8 weeks, the rest 25 patients were in the control group. Parameters of the OS werestudied. Results. The increase of total oxidative capacity, the decrease of total antioxidant capacity and the rise of levels of antibodies to oxidated LDL wererevealed in patients with DM2, DPN and AH. Antioxidant and anti-hypoxic effects of 400 mg/day of Actovegin were established in this group of patients.Conclusions. Actovegin impacts oxidative stress parameters and improves the clinical manifestation of diabetic polyneuropathy.

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Adenosine deaminase activity and zinc levels in the serum of patients with diabetes mellitus

Turkish Journal of Biochemistry ◽

10.1515/tjb-2016-0137 ◽

2016 ◽

Vol 41 (6) ◽

Author(s):

Rana Turkal ◽

Göksel Bahadır ◽

Yasemin Erdoğan Döventaş ◽

Goncagül Haklar ◽

Önder Şirikçi ◽

...

Keyword(s):

Diabetes Mellitus ◽

Adenosine Deaminase ◽

Type Ii Diabetes Mellitus ◽

Diabetic Patients ◽

Type I ◽

Adenosine Deaminase Activity ◽

Patients With Diabetes ◽

Patient Groups ◽

Zinc Levels ◽

Two Parameters

AbstractObjective:Adenosine deaminase (ADA) specifically catalyzes the deamination of adenosine which has been proved to play an important role in modulation of insulin action on glucose metabolism. Zinc is an essential micronutrient that is directly involved in the physiology of insulin and may be an important agent to activate the ADA. We aimed to evaluate serum ADA activity, zinc levels and the relationship between these two parameters in diabetes mellitus.Methods:We investigated serum ADA activity and zinc levels in type I (n = 100) and type II diabetes mellitus patients (n = 151).Results:ADA activities of diabetic patients were significantly elevated, whereas zinc levels were significantly lower than those of healthy controls (p < 0.001). Compared with the well-controlled diabetic patient groups (HbAConclusion:Elevated ADA activity in diabetic patients with poor glycemic control may be a useful marker for therapy modulation.

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The association of serum and vitreous adropin concentrations with diabetic retinopathy

Annals of Clinical Biochemistry International Journal of Laboratory Medicine ◽

10.1177/0004563218820359 ◽

2019 ◽

Vol 56 (2) ◽

pp. 253-258 ◽

Cited By ~ 3

Author(s):

Shipeng Li ◽

Jianling Sun ◽

Wenchao Hu ◽

Yan Liu ◽

Dan Lin ◽

...

Keyword(s):

Diabetes Mellitus ◽

Type 2 Diabetes ◽

Type 2 Diabetes Mellitus ◽

Diabetic Retinopathy ◽

Proliferative Diabetic Retinopathy ◽

Assay Method ◽

Enzyme Linked Immunosorbent Assay ◽

Control Group ◽

Diabetic Patients

Objective Adropin, a newly identified regulatory protein encoded by Enho gene, is correlated with insulin sensitivity and diabetes. The aim of this study is to determine whether serum and vitreous adropin concentrations are correlated with the presence of diabetic retinopathy. Methods A population of 165 patients with type 2 diabetes mellitus (52 without diabetic retinopathy, 69 with non-proliferative diabetic retinopathy and 44 patients with proliferative diabetic retinopathy) was enrolled in this study. The control group enrolled 68 healthy subjects who had underwent vitrectomy for retinal detachment. Serum and vitreous adropin concentrations were examined using enzyme-linked immunosorbent assay method. Results Control subjects had significantly higher serum and vitreous adropin concentrations compared with diabetic patients. Serum and vitreous adropin concentrations in proliferative diabetic retinopathy patients were significantly reduced compared with those in non-proliferative diabetic retinopathy patients and type 2 diabetes mellitus patients without diabetic retinopathy. In addition, there were lower serum and vitreous adropin concentrations in non-proliferative diabetic retinopathy patients compared with type 2 diabetes mellitus patients without diabetic retinopathy. Logistic regression analysis revealed that serum and vitreous adropin were associated with a decreased risk of type 2 diabetes mellitus and diabetic retinopathy. Conclusion Serum and vitreous adropin concentrations are negatively associated with the presence of diabetic retinopathy.

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In-shoe Plantar Pressure Distribution in Nonneuropathic Type 2 Diabetic Patients in Singapore

Journal of the American Podiatric Medical Association ◽

10.7547/1010509 ◽

2011 ◽

Vol 101 (6) ◽

pp. 509-516 ◽

Cited By ~ 1

Author(s):

Jasper W. K. Tong ◽

U. Rajendra Acharya ◽

Kuang C. Chua ◽

Peck H. Tan

Keyword(s):

Diabetes Mellitus ◽

Control Group ◽

Diabetic Patients ◽

Type 2 Diabetic Patients ◽

Maximum Peak ◽

Plantar Pressures ◽

Plantar Pressure Distribution ◽

Pressure Time ◽

Patients With Diabetes

Background: We sought to establish the in-shoe plantar pressure distribution during normal level walking in type 2 diabetic patients of Chinese, Indian, and Malay descent without clinical evidence of peripheral neuropathy. Methods: Thirty-five patients with type 2 diabetes mellitus without loss of tactile sensation and foot deformities and 38 nondiabetic individuals in a control group had in-shoe plantar pressures collected. Maximum peak pressure and peak pressure-time integral of each foot were analyzed as separate variables and were masked into 13 areas. Differences in pressure variables were assessed by analysis of covariance, adjusting for relevant covariates at the 95% confidence interval. Results: No significant differences were noted in maximum peak pressures after adjusting for sex, race, age, height, and body mass. However, patients with diabetes mellitus had significantly higher mean ± SD pressure-time integrals at the right whole foot (309.50 ± 144.17 kPa versus 224.06 ± 141.70 kPa, P < .05) and first metatarsal (198.65 ± 138.27 kPa versus 121.54 ± 135.91 kPa, P < .05) masked areas than did those in the control group after adjustment. Conclusions: Patients without clinical observable signs of foot deformity (implying absence of motor neuropathy) and sensory neuropathy had similar in-shoe maximum peak pressures as controls. This finding supported the notion that either component of neuropathy needs to be present before plantar pressures are elevated. Patients with diabetes mellitus demonstrated greater pressure-time integrals, implying that this variable might be the first clinical sign observable even before peripheral neuropathy could be tested. (J Am Podiatr Med Assoc 101(6): 509–516, 2011)

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Evaluation of α-klotho level in insulin dependent diabetes mellitus (IDDM) children

Journal of Pediatric Endocrinology and Metabolism ◽

10.1515/jpem-2019-0591 ◽

2020 ◽

Vol 33 (6) ◽

pp. 761-765

Author(s):

Fariba Tarhani ◽

Ghobad Heidari ◽

Alireza Nezami

Keyword(s):

Diabetes Mellitus ◽

Type I Diabetes ◽

Sandwich Elisa ◽

Serum Levels ◽

Dependent Diabetes Mellitus ◽

Control Group ◽

Case Group ◽

Diabetic Patients ◽

Type I ◽

And Control

AbstractObjectivesReduced levels of α-Klotho is associated with the pathogenesis of various diseases including diabetes. In type I diabetes, decrease in Klotho leads to apoptosis of β-cells of pancreases. The aim of this study was to evaluate the levels of α-Klotho in type I diabetic pediatric patients.MethodsIn this cross-sectional single centered study, 46 patients presenting type I diabetes mellitus (case group) and 78 control group under the age of 12, referred to our clinic were included in our study. Serum levels of soluble Klotho were measured by sandwich ELISA in case and control groups. Statistical analysis was conducted for the data recorded via questionnaire.ResultsMean age of the patients in the case and control group was 7.65 ± 3.09 and 7 ± 2.37, respectively. Type I diabetes patients had a significant reduction in the levels of serum Klotho, as compared to controls (p<0.001). However, gender and age-based comparison between patient and control group was not significant.ConclusionsThis study reports a significant decrease in the serum levels of α-Klotho in type 1 diabetic patients. Low levels of Klotho can be associated with diabetic nephropathy and other comorbidities in these patients.

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Proteomics Analysis of Gastric Cancer Patients with Diabetes Mellitus

Journal of Clinical Medicine ◽

10.3390/jcm10030407 ◽

2021 ◽

Vol 10 (3) ◽

pp. 407

Author(s):

Hugo Osório ◽

Cátia Silva ◽

Marta Ferreira ◽

Irene Gullo ◽

Valdemar Máximo ◽

...

Keyword(s):

Diabetes Mellitus ◽

Gastric Cancer ◽

Molecular Mechanisms ◽

Solid Phase ◽

Receptor Protein ◽

Cell Junction ◽

Control Group ◽

Diabetic Patients ◽

Proteomics Analysis ◽

Patients With Diabetes

Proteomics is a powerful approach to study the molecular mechanisms of cancer. In this study, we aim to characterize the proteomic profile of gastric cancer (GC) in patients with diabetes mellitus (DM) type 2. Forty GC tissue samples including 19 cases from diabetic patients and 21 cases from individuals without diabetes (control group) were selected for the proteomics analysis. Gastric tissues were processed following the single-pot, solid-phase-enhanced sample preparation approach—SP3 and enzymatic digestion with trypsin. The resulting peptides were analyzed by LC-MS Liquid Chromatography—Mass Spectrometry (LC-MS). The comparison of protein expression levels between GC samples from diabetic and non-diabetic patients was performed by label-free quantification (LFQ). A total of 6599 protein groups were identified in the 40 samples. Thirty-seven proteins were differentially expressed among the two groups, with 16 upregulated and 21 downregulated in the diabetic cohort. Statistical overrepresentation tests were considered for different annotation sets including the Gene Ontology(GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), Reactome, and Disease functional databases. Upregulated proteins in the GC samples from diabetic patients were particularly enriched in respiratory electron transport and alcohol metabolic biological processes, while downregulated proteins were associated with epithelial cancers, intestinal diseases, and cell–cell junction cellular components. Taken together, these results support the data already obtained by previous studies that associate diabetes with metabolic disorders and diabetes-associated diseases, such as Alzheimer’s and Parkinson’s, and also provide valuable insights into seven GC-associated protein targets, claudin-3, polymeric immunoglobulin receptor protein, cadherin-17, villin-1, transglutaminase-2, desmoglein-2, and mucin-13, which warrant further investigation.

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Systemic Erythropoietin Concentration and its Correlation with Stage of Diabetic Retinopathy

10.21203/rs.2.12950/v1 ◽

2019 ◽

Author(s):

Sofija Davidović ◽

Babić Nikola ◽

Jovanović Sandra ◽

Barišić Sava ◽

Grković Desanka ◽

...

Keyword(s):

Diabetes Mellitus ◽

Diabetic Retinopathy ◽

Serum Concentration ◽

Proliferative Diabetic Retinopathy ◽

Elevated Serum ◽

Average Concentration ◽

Control Group ◽

Diabetic Patients ◽

Study Results ◽

Patients With Diabetes

Abstract Summary: Background: Erythropoietin (Epo) is one of systemic angiogenic factors, and its role in ocular angiogenesis and in diabetic retinopathy (DR) is not yet fully understood. Latest research data reveal possible correlation of higher EPO concentrations of erythropoietin in blood and in the eye, with more severe of stages of DR. The main aim of this work was to examine the possible influence of serum concentrations of erythropoietin on the development and stages of diabetic retinopathy in patients with diabetes mellitus type 2. Methods: The research involved 90 patients examined at University Eye Clinic in Clinical Center of Vojvodina in Novi Sad, Serbia. First group comprised of 60 patients with diabetes mellitus lasting 10 years or more, with diabetic retinopathy. Second, control group, consisted of 30 healthy individuals. In the first group of 60 diabetic patients, 30 of them had non-proliferative diabetic retinopathy (NPDR), and 30 had proliferative diabetic retinopathy (PDR). Laboratory EPO serum levels were determined, and they were correlated to the stage of DR. Concentration of EPO was assessed by ELISA method at the end of the study. Results: The highest average concentration of EPO in serum (9.95 mIU/ml) was determined in group of diabetics with PDR. The lowest average concentration of EPO in serum (6.90 mIU/ml) was found in control group. The average concentration of Epo in serum in group of diabetics with NPDR was 7.00 mIU/ml. EPO concentration in serum was elevated in group of PDR, and it was directly proportional to the level of clinical stadium of PDR, being significantly higher in moderate and severe subgroup of PDR comparing to control healthy subjects, NPDR and mild PDR (h=9.858, p=0.007). Conclusions: Significantly elevated serum concentration of EPO in advanced stages of DR, and positive correlation between EPO serum concentration and clinical stadium of PDR, suggest that erythropoietin presents one of the important growth factors from blood, which plays role in retinal ischemia and angiogenesis in diabetic retinopathy, especially in the proliferative stage of this disease. Keywords: diabetic retinopathy; erythropoietin; glycated hemoglobin; non-proliferative diabetic retinopathy; proliferative diabetic retinopathy.

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Triglycerides Predict Plasma Fibronectin in Children with Type I Diabetes Mellitus Rather than Diabetes

Acta Medica (Hradec Kralove Czech Republic) ◽

10.14712/18059694.2019.54 ◽

2002 ◽

Vol 45 (1) ◽

pp. 33-37 ◽

Cited By ~ 1

Author(s):

Ayşe Binnur Erbağcı ◽

Mehmet Tarakçıoğlu ◽

Ahmet Erbağcı ◽

Mehmet Gözübüyük ◽

Necat Yılmaz ◽

...

Keyword(s):

Diabetes Mellitus ◽

Type I Diabetes ◽

Plasma Cholesterol ◽

Endothelial Activation ◽

Type I Diabetes Mellitus ◽

Control Group ◽

Type I ◽

Plasma Fibronectin ◽

Diabetic Children ◽

Adhesive Proteins

Fibronectins are adhesive proteins considered as markers of endothelial activation. Plasma fibronectin levels in diabetes mellitus (DM) have been found to be associated with atherosclerotic risk factors. This study was carried out to investigate plasma fibronectin and its relation with serum lipids, apolipoproteins AI, B100 and lp(a) in diabetic children. 35 children (19F/16M) with type I DM and 30 non-diabetic age and gender-matched controls were enrolled. Apolipoprotein and fibronectin concentrations were determined with nephelometric methods. Plasma fibronectin levels of the children with type I DM and the control group are not statistically different. HbA1c and triglycerides concentration are found to be significant predictors of plasma fibronectin in diabetic children, while effect of plasma cholesterol, apolipoprotein AI, B100 and lp(a) are insignificant. Diabetic children with triglycerides 1.13 mmol/l have elevated plasma fibronectin (median, 25th–75th percentiles; 29.6, 8.3–40.8 mg/dL) compared to the diabetic ≥19.9, 8.6–30.7 mg/dL, p<0,05) and non-diabetic children (16.6, 12.7–32.4 mg/dL, p<0.01) with triglycerides<1.13mmol/L. On the other hand plasma fibronectin concentrations of diabetic and non-diabetic children with high triglycerides are not significantly different. In conclusion our data does not support the concept that plasma fibronectin is elevated in type I diabetes mellitus at least in children, but high plasma triglycerides secondary to diabetes or not is associated with higher FNp concentrations which may have implications on atherogenesis. Plasma cholesterol, apolipoproteins AI, B100 and lp (a) are not significant determinants of FNp in type I diabetic children.

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“Keratrop” eye drops efficacy in dry eye syndrome treatment in diabetic patients

The Eye ◽

10.33791/2222-4408-2020-4-36-41 ◽

2020 ◽

Vol 22 (4(132)) ◽

pp. 36-41

Author(s):

F. A. Bakhritdinova ◽

F. A. Haydarova ◽

K. I. Narzikulova ◽

I. F. Nabieva

Keyword(s):

Diabetes Mellitus ◽

Dry Eye ◽

Tear Film ◽

Dry Eye Syndrome ◽

Control Group ◽

Diabetic Patients ◽

Eye Drops ◽

Artificial Tears ◽

Antidiabetic Treatment ◽

Patients With Diabetes

Significance. Prevalence of diabetes mellitus (DM) is increasing worldwide. People with diabetes are at higher risk to dry eye syndrome (DES). The increasing dependence of society on computers, air conditioning, and visual workload, etc. results in an increase in the manifestations of DES in diabetic patients. A significant part of the socially active population with diabetes requires prescription of drugs aimed at correcting disorders associated with both hyperglycemia and dry eyes. Conservative treatment of DES in case of diabetes includes prescription of artificial tears, metabolic, immunocorrecting, hormonal, antiallergic therapies as well as treatment of meibomian gland dysfunction (MGD). The tear substitutes, however, cannot always satisfy the needs of doctors and patients due to the imperfections of their formulas. Therefore, more and more new drugs are introduced to the market that require a comprehensive assessment.Purpose. To evaluate the clinical efficacy of Keratrop eye drops – a new artificial tear formulation – in dry eye syndrome treatment in patients with diabetes mellitus (DM).Methods. The study included 65 patients (130 eyes) with dry eye syndrome stages I and II associated with mild and moderate diabetes. These were adult patients of both sexes who had not previously received artificial tears as treatment and were undergoing either inpatient or domiciliary care. All studied patients provided a written informed consent and were divided into 2 groups by random sampling. The main group (35 patients) was receiving Keratrop instillations 2 times a day during 14 days, in the course of antidiabetic treatment. Instillations of “artificial tear” were not prescribed to patients of the control group (30 patients), and they only received antidiabetic treatment prescribed by the endocrinologist. Before and after treatment, all patients underwent standard ophthalmic examinations, meibography imaging, tear film assessment and filled in OSDI questionnaires.Results. The study showed that during treatment, in patients of the experimental group – with both I and II dry eye syndrome stages – a significant decrease in subjective and objective manifestations was observed, along with an improvement in the tear film stability. In the control group, positive dynamics was observed only in patients with the first stage of dry eye syndrome.Conclusion. Dry eye syndrome treatment in patients with diabetes should be comprehensive and must include both basic antidiabetic therapy and instillation of tear substitutes.

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Association of Glutathione–S-Transferase (GSTP1) Genetic Polymorphism in Iraqi Patients with Diabetes Mellitus Type2

Baghdad Science Journal ◽

10.21123/bsj.13.1.36-43 ◽

2016 ◽

Vol 13 (1) ◽

pp. 36-43

Author(s):

Baghdad Science Journal

Keyword(s):

Diabetes Mellitus ◽

Active Role ◽

Control Group ◽

Diabetic Patients ◽

Glutathione S Transferase ◽

The People ◽

Patients With Diabetes ◽

Glutathione S Transferases ◽

Polymerase Chain ◽

Control Study

Glutathione S-transferases (GSTs) are enzymes that included, in a more range of detoxifying reactions by conjugation of glutathione, to electrophilic material. Polymorphisms n the genes that responsible of GSTs affect, the function of the GSTs. GSTs play an active role in protection of cell against oxidative stress mechanism. Polymorphisms of GSTP1 at codon 105 amino acids forms GSTP1 important site for bind of hydrophobic electrophiles and the substitution of Ile/Val affect substrate specially catalytic activity of the enzyme and may correlate with reach to different diseases in human like diabetes mellitus type2 disease. Correlation between these polymorphisms and changes in the parameters file of diabetic patients has also been found, therefore, the results variation considerably among the studies; therefore, these control study was designed to leading to detecting know, as there are no studies on this performed in the people of Iraq. The polymerase chain reaction-restriction fragment length polymorphism was used to study GSTP1genetic polymorphism in 60 T2DM patients and 50 healthy individuals. Our results showed that presence of the GSTP1 heterozygous mutant allele Ile/Val was more common in subjects with T2DM than in the control group (40.00% and 32.00%, respectively; p = 0.01), as well as the found of the homozygous mutant of GSTP1 allele Val/Val was common in T2DM patient and not found in the control group (3.33% and 0.00%, respectively; p = 0.001).GSTP1 genotypes do not have an effect on blood lipids after infection with diabetes mellitus. Agarose gels used to determined genotypes according to the bands were that appeared in electrophoresis of gel.

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