Assessment of Serum L-Ficolin Level as Inflammatory Marker and Its Correlation with Serum C-Reactive Protein, D-Dimer and Ferritin in a Sample of Iraqi COVID-19 Patients

Subject: At the end of December 2019, a COVID-19 outbreak was reported for the first time in Wuhan, China. Which quickly expanded throughout China, then to 209 nations across America, Europe, Australia, and Asia. More than 50,000 people have died and over one million individuals have been afflicted worldwide, with the number fast rising. COVID-19 has been combated in various ways around the world. The gold standard for COVID-19 diagnosis is real-time fluorescence quantitative reverse transcriptase polymerase chain reaction (reverse transcriptase-polymerase chain reaction, RT-PCR) and viral gene sequencing. Currently, nasopharyngeal swabs from the upper respiratory tract are the most commonly employed nucleic acid detection samples. Objective of the Study: To estimate the level of ficolin in covid-19 patient in moderate and severe disease stage determination (series measurement and follow up) and find the diagnostic tool to early detect the virus to minimize the severity of the disease and further complication. Correlation of serum L-ficolin concentrations with Serum C-Reactive Protein, D-Dimer and Ferritin also to find Prognostic value of L-ficolin will be studied by corelating the treatment outcomes over one month with the three initial L-ficolin level. Materials and Methods: Sixty-two patients will be involved in the study from both sex male and female and different age group (18-80 yr.), the basal level of ficolin will be measure at time of admission of patient (time zero), then further serial measurement will be done on day 7 of admission for precise ficolin monitoring. Results: This study shows elevation of serum L-Ficolin level in survival and non-survival group. Conclusion: The results showed significant negative correlation between L-ficolin levels with the duration of illness (r = -0.377, p =0.020), however there were no significant correlation between L-ficolin levels with other patient‟s parameters.

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Detection of Occult Melanoma Cells in Paraffin-Embedded Histologically Negative Sentinel Lymph Nodes Using a Reverse Transcriptase Polymerase Chain Reaction Assay

Journal of Clinical Oncology ◽

10.1200/jco.2001.19.5.1437 ◽

2001 ◽

Vol 19 (5) ◽

pp. 1437-1443 ◽

Cited By ~ 50

Author(s):

Giuseppe Palmieri ◽

Paolo A. Ascierto ◽

Antonio Cossu ◽

Nicola Mozzillo ◽

Maria L. Motti ◽

...

Keyword(s):

Polymerase Chain Reaction ◽

Reverse Transcriptase ◽

Prognostic Significance ◽

Primary Melanoma ◽

Disease Stage ◽

Occult Metastasis ◽

Rt Pcr ◽

Chain Reaction ◽

Pcr Assay ◽

Polymerase Chain

PURPOSE: Detection of occult metastasis before the development of clinical disease could allow more accurate staging, appropriate follow-up procedures, and adjuvant therapies in patients with malignant melanoma (MM). The sentinel lymph node (SLN) has been proposed as a reliable predictor of metastatic disease in the lymphatic basin draining the primary melanoma. In this study, we screened both paraffin-embedded SLNs and peripheral-blood (PB) samples from MM patients at various stage of disease using a multimarker reverse transcriptase polymerase chain reaction (RT-PCR) assay. The prognostic significance of the presence of PCR-positive markers was also evaluated. PATIENTS AND METHODS: Total RNA was obtained from paraffin-embedded SLN sections and PB samples of 75 MM patients. RT-PCR was performed using tyrosinase and MelanA/MART1 as melanoma-associated markers. Radiolabeled PCR products were analyzed on denaturing polyacrylamide gels. RESULTS: Good sensitivity of the RT-PCR assay on archival tissues was demonstrated after comparison of RT-PCR results on frozen and paraffin-embedded SLNs from 16 MM patients. Significant correlation between the disease stage and marker expression in both PB and SLN samples was observed; the highest value was for patients who were positive for both markers in SLN (P = .006). Progression of disease was significantly associated with the total number of PCR-positive markers in both PB (P = .034) and SLN (P = .001) samples. CONCLUSION: Although sensitivity is lowered by the use of paraffin-embedded specimens, our data indicate that RT-PCR analysis of serial sections from archival SLNs may be helpful in improving detection of occult micrometastases, thus improving staging of patients with melanoma.

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Uji Diagnostik C-Reactive Protein pada Pneumonia Bakteri Komunitas Anak

Sari Pediatri ◽

10.14238/sp17.5.2016.391-395 ◽

2016 ◽

Vol 17 (5) ◽

pp. 391

Author(s):

Ikhsan Marzony ◽

Finny Fitry Yani ◽

Efrida Efrida

Keyword(s):

Polymerase Chain Reaction ◽

C Reactive Protein ◽

Chain Reaction ◽

Cross Sectional ◽

Reactive Protein ◽

Polymerase Chain

Latar belakang. Polymerase chain reaction (PCR) merupakan teknik untuk menentukan agen penyebab pneumonia dengan sensitivitas dan spesifisitas yang tinggi, tetapi harganya mahal dan tidak tersedia di semua tempat. C-reactive protein (CRP) juga dapat digunakan untuk memprediksi infeksi bakteri dan memiliki keunggulan yang lain, yakni harganya yang murah dan hampir tersedia di semua laboratorium.Tujuan. Mengetahui sensitivitas, spesifisitas, nilai prediksi positif, dan nilai prediksi negatif CRP pada pneumonia bakteri komunitas anak.Metode. Penelitian cross sectional pada 62 subjek di Bagian Ilmu Kesehatan Anak RS. Dr. M. Djamil Padang dari Desember 2013 sampai Oktober 2014. Subjek dipilih dengan teknik konsekutif. Dilakukan uji CRP dan teknik PCR sebagai baku emas. Duapuluh tiga sampel diekslusi karena menderita penyakit jantung bawaan (7), sepsis (6), telah mendapatkan antibiotik (4), dan orang tua menolak pemeriksaan (6) sehingga total sampel menjadi 39 anak.Hasil. Sebagian besar subjek penelitian adalah laki-laki (59%) dan kelompok umur terbanyak 2-11 bulan (48,7%). Sensitivitas CRP dengan cut off point 8 mg/L adalah 51,6%, spesifisitas 75%, nilai prediksi positif 88,8%, nilai prediksi negatif 28,6%. Pada cut off point 10 mg/L, sensitivitas CRP adalah 41,9%, spesifisitas 87,5%, nilai prediksi positif 92,9%, nilai prediksi negatif 28%.Kesimpulan. C-reactive protein tidak dapat digunakan sebagai uji tapis terhadap pneumonia bakteri komunitas anak.

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DETERMINATION OF DIAGNOSTIC ACCURACY OF BIOCHEMICAL PARAMETERS (CRP, LDH & FERRITIN) IN THE DIAGNOSIS OF COVID-19 IN SUSPECTED COVID CASES

Pakistan Armed Forces Medical Journal ◽

10.51253/pafmj.v71i5.5749 ◽

2021 ◽

Vol 71 (5) ◽

pp. 1722-26

Author(s):

Tayyaba Ashiq ◽

Abdus Sattar ◽

Nasir Uddin ◽

Qamar Bashir ◽

Sajida Shaheen ◽

...

Keyword(s):

Polymerase Chain Reaction ◽

Lactate Dehydrogenase ◽

Diagnostic Accuracy ◽

Biochemical Parameters ◽

C Reactive Protein ◽

Chain Reaction ◽

Reactive Protein ◽

Poor Predictor ◽

Lactate Dehydrogenase C ◽

Polymerase Chain

Objective: To determine the diagnostic accuracy of the Lactate Dehydrogenase, C-Reactive Protein and Ferritin in suspected patients of COVID-19. Study Design: Cross-sectional validation study. Place and Duration of Study: Pathology department of Combined Military Hospital Lahore in the month of May 2020. Methodology: We included 101 adult (>18 years) symptomatic suspected COVID-19 patients of both genders. Children, pregnant women and asymptomatic patients were excluded from study. Age, gender and results of Reverse Transcriptase Polymerase Chain Reaction, Lactate Dehydrogenase, C-Reactive Protein, ferritin were recorded. Results: Lactate Dehydrodenase had highest sensitivity (75%) with positive predictive value of 71.6% and diagnostic accuracy of 65.3% among three biochemical parameters studied. Receiver Operator Characteristic curve was studied. Area under curve of Lactate Dehydrogenase (AUC=0.65) and Ferritin (AUC=0.59) reflected their ability to prognosticate the presence of COVID19 disease. However, C-Reactive Protein (AUC=0.42) appeared to be a poor predictor of the disease. Conclusion: Raised serum Lactate Dehydrogenase (>490 U/L) and Ferritin (>152 ng/L) levels can be used to predict the Reverse Transcriptase Polymerase Chain Reaction positivity for COVID-19 in the population of suspected patients of COVID19. However, C-Reactive Protein is a poor predictor of COVID-19.

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Experience with the use of siltuximab in patients with SARS-CoV-2 infection

Revista Española de Quimioterapia ◽

10.37201/req/045.2021 ◽

2021 ◽

Author(s):

Fernanda Meira ◽

Laia Albiach ◽

Cristina Carbonell ◽

José-Ángel Martín-Oterino ◽

Mercedes Martín-Ordiales ◽

...

Keyword(s):

Polymerase Chain Reaction ◽

Nasopharyngeal Swab ◽

C Reactive Protein ◽

Median Dose ◽

First Line ◽

Chain Reaction ◽

Positive Polymerase Chain Reaction ◽

Reactive Protein ◽

Polymerase Chain ◽

Hospital Clinic

Objectives. The study aims to describe characteristics and clinical outcome of patients with SARS-CoV-2 infection that received siltuximab according to a protocol that aimed to early block the activity of IL-6 to avoid the progression of the inflammatory flare. Patients and methods. Retrospective review of the first 31 patients with SARS-CoV-2 treated with siltuximab, in Hospital Clinic of Barcelona or Hospital Universitario Salamanca, from March to April 2020 with positive polymerase-chain reaction (PCR) from a nasopharyngeal swab. Results. The cohort included 31 cases that received siltuximab with a median (IQR) age of 62 (56-71) and 71% were males. The most frequent comorbidity was hypertension (48%). The median dose of siltuximab was 800 mg ranging between 785 and 900 mg. 7 patients received siltuximab as a salvage therapy after one dose of tocilizumab. At the end of the study, a total of 26 (83.9) patients had been discharged alive and the mortality rate was 16.1% but only 1 out of 24 that received siltuximab as a first line option (4%). Conclusions. Siltuximab is a well-tolerated alternative to tocilizumab when administered as a first line option in patients with COVID-19 pneumonia within the first 10 days from symptoms onset and high C-reactive protein.

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Respiratory and non-respiratory manifestations in children admitted with COVID 19 in Rio de Janeiro city, Brazil

10.1101/2020.12.29.20248994 ◽

2021 ◽

Author(s):

AR Araujo da Silva ◽

CGB Fonseca ◽

JLPS Miranda ◽

BV Travassos ◽

CR Baião ◽

...

Keyword(s):

Polymerase Chain Reaction ◽

Length Of Stay ◽

Respiratory Symptoms ◽

C Reactive Protein ◽

Chain Reaction ◽

Serological Tests ◽

Main Symptom ◽

Reactive Protein ◽

Polymerase Chain ◽

Janeiro City

AbstractIntroductionCOVID 19 is still a challenge in pediatrics due to variety of symptoms and different presentationsAimTo describe clinical, laboratorial and treatment of confirmed COVID-19 pediatric admitted in hospitals.MethodsA retrospective study was conducted in children (0-18 years), admitted between March and November 15, 2020, with confirmed COVID-19 by reverse transcription polymerase chain reaction or serological tests. Clinical data about symptoms, laboratorial exams and treatments were analysed. Patients were evaluated according predominant (PRS) or non-predominant respiratory symptoms (non-PRS)ResultsSixty-four patients were evaluated, being the median age 5.6 years. Forty-seven (73.4%) children were admitted with PRS and 17 (26.4%) with non-PRS. The main symptoms in the PRS group were fever in 74.5% of children and cough in 66%; and fever in 76.5% and edema/cavitary effusion in 29.4% in the non-PRS group. The median of C-reactive protein (in mg/dl) was 2.5 in the PRS group and 6.1 in the non-PRS group. Antibiotics were used in 85.1% of the PRS group and 94.1% of non-group. Comorbidity was present in 30/47 (63.8%) of PRS group and 8/17 (47.1%) of non-PRS group (p=0.22). Length of stay until 7 days in patients with comorbidity was present in 27/64 (42.1%) and more than 7 days in 11/64 (17.1%) (p= 0.2)ConclusionNon-PRS represented more than one quarter of admitted patients. Fever was the main symptom detected, elevated CRP was frequent and antibiotics were commonly prescribed. Comorbidity was found in both groups and his presence was not associated with a longer length of stay.

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