scholarly journals Respiratory and non-respiratory manifestations in children admitted with COVID 19 in Rio de Janeiro city, Brazil

2021 ◽  
Author(s):  
AR Araujo da Silva ◽  
CGB Fonseca ◽  
JLPS Miranda ◽  
BV Travassos ◽  
CR Baião ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Length Of Stay ◽  
Respiratory Symptoms ◽  
C Reactive Protein ◽  
Chain Reaction ◽  
Serological Tests ◽  
Main Symptom ◽  
Reactive Protein ◽  
Polymerase Chain ◽  
Janeiro City

AbstractIntroductionCOVID 19 is still a challenge in pediatrics due to variety of symptoms and different presentationsAimTo describe clinical, laboratorial and treatment of confirmed COVID-19 pediatric admitted in hospitals.MethodsA retrospective study was conducted in children (0-18 years), admitted between March and November 15, 2020, with confirmed COVID-19 by reverse transcription polymerase chain reaction or serological tests. Clinical data about symptoms, laboratorial exams and treatments were analysed. Patients were evaluated according predominant (PRS) or non-predominant respiratory symptoms (non-PRS)ResultsSixty-four patients were evaluated, being the median age 5.6 years. Forty-seven (73.4%) children were admitted with PRS and 17 (26.4%) with non-PRS. The main symptoms in the PRS group were fever in 74.5% of children and cough in 66%; and fever in 76.5% and edema/cavitary effusion in 29.4% in the non-PRS group. The median of C-reactive protein (in mg/dl) was 2.5 in the PRS group and 6.1 in the non-PRS group. Antibiotics were used in 85.1% of the PRS group and 94.1% of non-group. Comorbidity was present in 30/47 (63.8%) of PRS group and 8/17 (47.1%) of non-PRS group (p=0.22). Length of stay until 7 days in patients with comorbidity was present in 27/64 (42.1%) and more than 7 days in 11/64 (17.1%) (p= 0.2)ConclusionNon-PRS represented more than one quarter of admitted patients. Fever was the main symptom detected, elevated CRP was frequent and antibiotics were commonly prescribed. Comorbidity was found in both groups and his presence was not associated with a longer length of stay.

scholarly journals Uji Diagnostik C-Reactive Protein pada Pneumonia Bakteri Komunitas Anak

Sari Pediatri ◽  
2016 ◽  
Vol 17 (5) ◽  
pp. 391
Author(s):  
Ikhsan Marzony ◽  
Finny Fitry Yani ◽  
Efrida Efrida
Keyword(s):  
Polymerase Chain Reaction ◽  
C Reactive Protein ◽  
Chain Reaction ◽  
Cross Sectional ◽  
Reactive Protein ◽  
Polymerase Chain

Latar belakang. Polymerase chain reaction (PCR) merupakan teknik untuk menentukan agen penyebab pneumonia dengan sensitivitas dan spesifisitas yang tinggi, tetapi harganya mahal dan tidak tersedia di semua tempat. C-reactive protein (CRP) juga dapat digunakan untuk memprediksi infeksi bakteri dan memiliki keunggulan yang lain, yakni harganya yang murah dan hampir tersedia di semua laboratorium.Tujuan. Mengetahui sensitivitas, spesifisitas, nilai prediksi positif, dan nilai prediksi negatif CRP pada pneumonia bakteri komunitas anak.Metode. Penelitian cross sectional pada 62 subjek di Bagian Ilmu Kesehatan Anak RS. Dr. M. Djamil Padang dari Desember 2013 sampai Oktober 2014. Subjek dipilih dengan teknik konsekutif. Dilakukan uji CRP dan teknik PCR sebagai baku emas. Duapuluh tiga sampel diekslusi karena menderita penyakit jantung bawaan (7), sepsis (6), telah mendapatkan antibiotik (4), dan orang tua menolak pemeriksaan (6) sehingga total sampel menjadi 39 anak.Hasil. Sebagian besar subjek penelitian adalah laki-laki (59%) dan kelompok umur terbanyak 2-11 bulan (48,7%). Sensitivitas CRP dengan cut off point 8 mg/L adalah 51,6%, spesifisitas 75%, nilai prediksi positif 88,8%, nilai prediksi negatif 28,6%. Pada cut off point 10 mg/L, sensitivitas CRP adalah 41,9%, spesifisitas 87,5%, nilai prediksi positif 92,9%, nilai prediksi negatif 28%.Kesimpulan. C-reactive protein tidak dapat digunakan sebagai uji tapis terhadap pneumonia bakteri komunitas anak.

scholarly journals DETERMINATION OF DIAGNOSTIC ACCURACY OF BIOCHEMICAL PARAMETERS (CRP, LDH & FERRITIN) IN THE DIAGNOSIS OF COVID-19 IN SUSPECTED COVID CASES

2021 ◽  
Vol 71 (5) ◽  
pp. 1722-26
Author(s):  
Tayyaba Ashiq ◽  
Abdus Sattar ◽  
Nasir Uddin ◽  
Qamar Bashir ◽  
Sajida Shaheen ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Lactate Dehydrogenase ◽  
Diagnostic Accuracy ◽  
Biochemical Parameters ◽  
C Reactive Protein ◽  
Chain Reaction ◽  
Reactive Protein ◽  
Poor Predictor ◽  
Lactate Dehydrogenase C ◽  
Polymerase Chain

Objective: To determine the diagnostic accuracy of the Lactate Dehydrogenase, C-Reactive Protein and Ferritin in suspected patients of COVID-19. Study Design: Cross-sectional validation study. Place and Duration of Study: Pathology department of Combined Military Hospital Lahore in the month of May 2020. Methodology: We included 101 adult (>18 years) symptomatic suspected COVID-19 patients of both genders. Children, pregnant women and asymptomatic patients were excluded from study. Age, gender and results of Reverse Transcriptase Polymerase Chain Reaction, Lactate Dehydrogenase, C-Reactive Protein, ferritin were recorded. Results: Lactate Dehydrodenase had highest sensitivity (75%) with positive predictive value of 71.6% and diagnostic accuracy of 65.3% among three biochemical parameters studied. Receiver Operator Characteristic curve was studied. Area under curve of Lactate Dehydrogenase (AUC=0.65) and Ferritin (AUC=0.59) reflected their ability to prognosticate the presence of COVID19 disease. However, C-Reactive Protein (AUC=0.42) appeared to be a poor predictor of the disease. Conclusion: Raised serum Lactate Dehydrogenase (>490 U/L) and Ferritin (>152 ng/L) levels can be used to predict the Reverse Transcriptase Polymerase Chain Reaction positivity for COVID-19 in the population of suspected patients of COVID19. However, C-Reactive Protein is a poor predictor of COVID-19.

scholarly journals Assessment of Serum L-Ficolin Level as Inflammatory Marker and Its Correlation with Serum C-Reactive Protein, D-Dimer and Ferritin in a Sample of Iraqi COVID-19 Patients

2021 ◽  
Vol 23 (08) ◽  
pp. 107-116
Author(s):  
Layth Taha Abdulhussein ◽  
◽  
Dr. Mohammed Abdullatif ◽  
Dr. Aref Sami Malik ◽  
◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Reverse Transcriptase ◽  
Disease Stage ◽  
Viral Gene ◽  
Upper Respiratory Tract ◽  
C Reactive Protein ◽  
Chain Reaction ◽  
Reactive Protein ◽  
Polymerase Chain ◽  
D Dimer

Subject: At the end of December 2019, a COVID-19 outbreak was reported for the first time in Wuhan, China. Which quickly expanded throughout China, then to 209 nations across America, Europe, Australia, and Asia. More than 50,000 people have died and over one million individuals have been afflicted worldwide, with the number fast rising. COVID-19 has been combated in various ways around the world. The gold standard for COVID-19 diagnosis is real-time fluorescence quantitative reverse transcriptase polymerase chain reaction (reverse transcriptase-polymerase chain reaction, RT-PCR) and viral gene sequencing. Currently, nasopharyngeal swabs from the upper respiratory tract are the most commonly employed nucleic acid detection samples. Objective of the Study: To estimate the level of ficolin in covid-19 patient in moderate and severe disease stage determination (series measurement and follow up) and find the diagnostic tool to early detect the virus to minimize the severity of the disease and further complication. Correlation of serum L-ficolin concentrations with Serum C-Reactive Protein, D-Dimer and Ferritin also to find Prognostic value of L-ficolin will be studied by corelating the treatment outcomes over one month with the three initial L-ficolin level. Materials and Methods: Sixty-two patients will be involved in the study from both sex male and female and different age group (18-80 yr.), the basal level of ficolin will be measure at time of admission of patient (time zero), then further serial measurement will be done on day 7 of admission for precise ficolin monitoring. Results: This study shows elevation of serum L-Ficolin level in survival and non-survival group. Conclusion: The results showed significant negative correlation between L-ficolin levels with the duration of illness (r = -0.377, p =0.020), however there were no significant correlation between L-ficolin levels with other patient‟s parameters.

scholarly journals Trends of COVID-19 pediatric admissions number during the first 24 weeks of COVID-19 vaccination in Rio de Janeiro, Brazil

2021 ◽  
Author(s):  
Andre Ricardo Araujo da Silva ◽  
Monica Del Monaco Esteves ◽  
Bernardo Rodrigues Rosa de Carvalho ◽  
Cristina Vieira Souza ◽  
Cristiane Henriques Teixeira
Keyword(s):  
Polymerase Chain Reaction ◽  
Retrospective Study ◽  
Rio De Janeiro ◽  
Chain Reaction ◽  
Serological Tests ◽  
Number Of Children ◽  
Pediatric Hospitals ◽  
Polymerase Chain ◽  
All Diseases ◽  
Janeiro City

Objective: To describe trends of COVID-19 pediatric admissions number during the first 24 weeks of COVID-19 vaccination. Design: A retrospective study was conducted in children (0-18 years), admitted in two pediatric hospitals of Rio de Janeiro city, between January 17 and July 3, 2021 with confirmed COVID-19 by reverse transcription polymerase chain reaction or serological tests. Trends of COVID-19 pediatric admissions number during the first 24 weeks of COVID-19 vaccination in Rio de Janeiro, Brazil and the pre-vaccine period were measured by linear regression. Participants: Children admitted in pediatric hospitals in Rio de Janeiro, city, Brazil Results: The number of total admitted patients (with all diseases) were 5340 during the pre-vaccine period, being 94 (1.8%) of them with confirmed COVID-19, and 4182 children admitted during the vaccine period, with 86 confirmed COVID-19 patients (2.1 %) (p=0.29). Media of cases admitted per/week were 2.02 in pre-vaccine period and 3.6 during the first 24 weeks of COVID vaccination (p=0.009). One death was reported in the pre-vaccine period and four in the vaccine period (p=0.14). Trends of increase in the number of admitted cases were verified both in the pre-vaccine period as in the vaccine period, being more expressive in the last one. Conclusion: There was trend of increase in number of children admitted with confirmed COVID-19 during the first 24 weeks of COVID-vaccination in Rio de Janeiro, city. Considering that few people were fully vaccinated, reducing of number of admitted children with confirmed COVID-19 was not verified.

scholarly journals Experience with the use of siltuximab in patients with SARS-CoV-2 infection

2021 ◽  
Author(s):  
Fernanda Meira ◽  
Laia Albiach ◽  
Cristina Carbonell ◽  
José-Ángel Martín-Oterino ◽  
Mercedes Martín-Ordiales ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Nasopharyngeal Swab ◽  
C Reactive Protein ◽  
Median Dose ◽  
First Line ◽  
Chain Reaction ◽  
Positive Polymerase Chain Reaction ◽  
Reactive Protein ◽  
Polymerase Chain ◽  
Hospital Clinic

Objectives. The study aims to describe characteristics and clinical outcome of patients with SARS-CoV-2 infection that received siltuximab according to a protocol that aimed to early block the activity of IL-6 to avoid the progression of the inflammatory flare. Patients and methods. Retrospective review of the first 31 patients with SARS-CoV-2 treated with siltuximab, in Hospital Clinic of Barcelona or Hospital Universitario Salamanca, from March to April 2020 with positive polymerase-chain reaction (PCR) from a nasopharyngeal swab. Results. The cohort included 31 cases that received siltuximab with a median (IQR) age of 62 (56-71) and 71% were males. The most frequent comorbidity was hypertension (48%). The median dose of siltuximab was 800 mg ranging between 785 and 900 mg. 7 patients received siltuximab as a salvage therapy after one dose of tocilizumab. At the end of the study, a total of 26 (83.9) patients had been discharged alive and the mortality rate was 16.1% but only 1 out of 24 that received siltuximab as a first line option (4%). Conclusions. Siltuximab is a well-tolerated alternative to tocilizumab when administered as a first line option in patients with COVID-19 pneumonia within the first 10 days from symptoms onset and high C-reactive protein.

scholarly journals Comparison of multiplex real-time polymerase chain reaction with serological tests and culture for diagnosing human brucellosis

2019 ◽  
Vol 12 (3) ◽  
pp. 337-342 ◽  
Author(s):  
Tuba Dal ◽  
Soner Sertan Kara ◽  
Aytekin Cikman ◽  
Cigdem Eda Balkan ◽  
Ziya Cibali Acıkgoz ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
Human Brucellosis ◽  
Chain Reaction ◽  
Serological Tests ◽  
Polymerase Chain

scholarly journals USE OF THE POLYMERASE CHAIN REACTION FOR THE DIAGNOSIS OF ASYMPTOMATIC Leishmania INFECTION IN A VISCERAL LEISHMANIASIS-ENDEMIC AREA

2013 ◽  
Vol 55 (2) ◽  
pp. 101-104 ◽  
Author(s):  
Luciana Almeida Silva ◽  
Héctor Dardo Romero ◽  
Aline Fagundes ◽  
Nédia Nehme ◽  
Otávio Fernandes ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Visceral Leishmaniasis ◽  
Endemic Area ◽  
Asymptomatic Infection ◽  
Control Measures ◽  
Skin Tests ◽  
Leishmania Infection ◽  
Chain Reaction ◽  
Serological Tests ◽  
Polymerase Chain

The diagnosis of asymptomatic infection with Leishmania (Leishmania) chagasi has become more important over recent years. Expansion of visceral leishmaniasis might be associated with other routes of transmission such as transfusion, congenital or even vector transmission, and subjects with asymptomatic infection are potential reservoirs. Moreover, the identification of infection may contribute to the management of patients with immunosuppressive conditions (HIV, transplants, use of immunomodulators) and to the assessment of the effectiveness of control measures. In this study, 149 subjects living in a visceral leishmaniasis endemic area were evaluated clinically and submitted to genus-specific polymerase chain reaction (PCR), serological testing, and the Montenegro skin test. Forty-nine (32.9%) of the subjects had a positive PCR result and none of them developed the disease within a follow-up period of three years. No association was observed between the results of PCR, serological and skin tests. A positive PCR result in subjects from the endemic area did not indicate a risk of progression to visceral leishmaniasis and was not associated with a positive result in the serological tests.

scholarly journals SARS–CoV-2 isolation from an appendix

2020 ◽  
Vol 2020 (8) ◽  
Author(s):  
Shahzaib Ahmad ◽  
Rahim Nadeem Ahmed ◽  
Poonam Jani ◽  
Mattee Ullah ◽  
Hossam Aboulgheit
Keyword(s):  
Polymerase Chain Reaction ◽  
Abdominal Pain ◽  
Respiratory Symptoms ◽  
Tissue Sample ◽  
Gastrointestinal Symptoms ◽  
Shortness Of Breath ◽  
Chain Reaction ◽  
Histological Features ◽  
First Case ◽  
Polymerase Chain

Abstract Efforts to recognize SARS–CoV-2 infection have focused on respiratory symptoms such as cough and shortness of breath. Although it is also well known that SARS–CoV-2 infection can cause gastrointestinal symptoms such as abdominal pain, nausea, vomiting and diarrhoea, there are emerging reports of SARS–CoV-2 infection causing surgical pathology. We present the first case report of SARS–CoV-2 infection directly causing acute appendicitis, first suspected due to highly atypical histological features and later confirmed as polymerase chain reaction positive appendicular tissue sample.

scholarly journals Impact of Respiratory Viral Panel Polymerase Chain Reaction Assay Turnaround Time on Length of Stay and Antibiotic Use in Patients With Respiratory Viral Illnesses

2017 ◽  
Vol 52 (9) ◽  
pp. 640-644 ◽  
Author(s):  
Sebastian Choi ◽  
Rubiya Kabir ◽  
Pranisha Gautam-Goyal ◽  
Prashant Malhotra
Keyword(s):  
Polymerase Chain Reaction ◽  
Length Of Stay ◽  
Retrospective Chart Review ◽  
Antibiotic Use ◽  
Turnaround Time ◽  
Chain Reaction ◽  
Time Period ◽  
Significant Difference ◽  
Before And After ◽  
Polymerase Chain

Background: Respiratory viral illnesses account for many hospitalizations and inappropriate antibiotic use. Respiratory viral panels by polymerase chain reaction (RVP-PCR) provide a reliable means of diagnosis. In 2015, the RVP-PCR assay at our institution was switched from respiratory viral panel (RVP) to rapid respiratory panel (rapid RP), which has a faster turnaround time (24 hours vs 12 hours, respectively). The purpose of this study was to evaluate the effect of RVP-PCR tests on duration of antibiotic use and length of stay (LOS) in hospitalized patients. Methods: We performed a retrospective chart review of patients who had a RVP-PCR ordered within a 1-year time period before and after the assay switch. Patients who were pregnant, had received antibiotics within 30 days prior to admission, were not discharged, or had not completed antibiotics by end of study period were excluded. Results: Data were obtained from a total of 140 patients (70 in each group). Of these, 25 (35.7%) in the RVP group and 28 (40.0%) in the rapid RP group had a positive result. The median LOS was 4.5 days (IQR, 3-9 days) in the RVP group and 5 days (IQR, 3-9 days) in the rapid RP group ( P = .78). The median duration of antibiotic use was 4 days (IQR, 2-7 days) in the RVP group and 5 days (IQR, 1-7 days) in the rapid RP group ( P = .8). Conclusion: Despite faster turnaround time, there was no significant difference in duration of antibiotic use, or LOS between the RVP and rapid RP groups.

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